pbs medium  (Beyotime)

 
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  • 97
    Name:
    Cell Culture Freezing Medium
    Description:

    Catalog Number:
    C0210
    Price:
    75.0
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    Structured Review

    Beyotime pbs medium
    Percentage of BrdU positive eosinophils of <t>BALF,</t> blood and bone marrow from wild type and IL-25 KO mouse models that were sensitized and challenged with OVA or <t>PBS</t> (control) (*p

    https://www.bioz.com/result/pbs medium/product/Beyotime
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbs medium - by Bioz Stars, 2021-04
    97/100 stars

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    1) Product Images from "Interleukin-25 and eosinophils progenitor cell mobilization in allergic asthma"

    Article Title: Interleukin-25 and eosinophils progenitor cell mobilization in allergic asthma

    Journal: Clinical and Translational Allergy

    doi: 10.1186/s13601-018-0190-2

    Percentage of BrdU positive eosinophils of BALF, blood and bone marrow from wild type and IL-25 KO mouse models that were sensitized and challenged with OVA or PBS (control) (*p
    Figure Legend Snippet: Percentage of BrdU positive eosinophils of BALF, blood and bone marrow from wild type and IL-25 KO mouse models that were sensitized and challenged with OVA or PBS (control) (*p

    Techniques Used:

    Eosinophil percentage of BALF, blood and bone marrow from wild type and IL-25 KO mouse models that were sensitized and challenged with OVA or PBS (control) (*p
    Figure Legend Snippet: Eosinophil percentage of BALF, blood and bone marrow from wild type and IL-25 KO mouse models that were sensitized and challenged with OVA or PBS (control) (*p

    Techniques Used:

    Related Articles

    Nitric Oxide Assay:

    Article Title: Iptakalim ameliorates hypoxia-impaired human endothelial colony-forming cells proliferation, migration, and angiogenesis via Akt/eNOS pathways
    Article Snippet: .. Herein, the levels of NO2− /NO3− in the culture medium were determined by a commercially available Nitric Oxide Assay Kit (Beyotime Institute of Biotechnology, Nanjing, China) according to the manufacturer’s instructions. .. The absorbency was measured at 540 nm with a microplate reader (Thermo Scientific, CA, USA).

    Stable Transfection:

    Article Title: BxPC-3-Derived Small Extracellular Vesicles Induce FOXP3+ Treg through ATM-AMPK-Sirtuins-Mediated FOXOs Nuclear Translocations.
    Article Snippet: Generation of tdTomato/EGFP-BxPC-3 cellssEVs-CD63-toTomato virus and sEVs-TGF-β1-EGFP virus were obtained from S Shanghai Medical Biotechnology Co., Ltd. For labeling CD63 (or TGF-β1) with fluorescence, BxPC-3 cells were transfected with a CMV promoter-driving tdTomato-tagged CD63 gene (or EGFP-tagged TGF-β1 gene) using lentivirus vector system. .. Culture medium added with 1µg/mL puromycin (Beyotime) was using to purify stably transfected BxPC-3 cells. .. Purification and Verification of sEVssEVs were purified from serum-free supernatants of human HPSC, BxPC-3, PANC-1, tdTomato-BxPC- 3, Ctrl/CD63-BxPC-3, or TGF-β1/CD63-BxPC-3 cells.

    Transfection:

    Article Title: BxPC-3-Derived Small Extracellular Vesicles Induce FOXP3+ Treg through ATM-AMPK-Sirtuins-Mediated FOXOs Nuclear Translocations.
    Article Snippet: Generation of tdTomato/EGFP-BxPC-3 cellssEVs-CD63-toTomato virus and sEVs-TGF-β1-EGFP virus were obtained from S Shanghai Medical Biotechnology Co., Ltd. For labeling CD63 (or TGF-β1) with fluorescence, BxPC-3 cells were transfected with a CMV promoter-driving tdTomato-tagged CD63 gene (or EGFP-tagged TGF-β1 gene) using lentivirus vector system. .. Culture medium added with 1µg/mL puromycin (Beyotime) was using to purify stably transfected BxPC-3 cells. .. Purification and Verification of sEVssEVs were purified from serum-free supernatants of human HPSC, BxPC-3, PANC-1, tdTomato-BxPC- 3, Ctrl/CD63-BxPC-3, or TGF-β1/CD63-BxPC-3 cells.

    Article Title: Downregulation of circular RNA hsa_circ_0000735 boosts prostate cancer sensitivity to docetaxel via sponging miR-7
    Article Snippet: 18S ribosomal RNA (rRNA) or U6 snRNA were used as the internal reference for cytoplasm and nucleus, respectively. .. Cell Counting Kit-8 (CCK-8) assay For cytotoxic and half-maximal inhibitory concentration (IC50) value assessment, the transfected PC-3/DTX and DU145/DTX cells (2.0 × 103 ) were cultured in cell medium with different concentrations of DTX (5, 10, 20, 40, 80, or 160 nM) for 48 h. For cell viability analysis, the transfected PC-3/DTX and DU145/DTX cells (2.0 × 103) were seeded into 96-well plates with cell medium (10 nM DTX) for 24 h, 48 h, or 72 h. Thereafter, the CCK-8 solution (10 μL, Beyotime, Shanghai, China) was added to each well and incubated for 2 h. The absorbance at 450 nm was measured with a microplate reader (Bio-Rad, Hercules, CA, USA). .. Cell colony formation assay The transfected PC-3/DTX and DU145/DTX cells (1.0 × 102 cells/well) were seeded into 6-well plates and then treated with 10 nM DTX when the cell was attached to the wall.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Knockdown of circ-FANCA alleviates LPS-induced HK2 cell injury via targeting miR-93-5p/OXSR1 axis in septic acute kidney injury
    Article Snippet: Cell cycle distribution at different phases (G0/G1, S and G2/M) was assessed using flow cytometer (BD Biosciences). .. Enzyme-linked immunosorbent assay (ELISA)The release of inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor α (TNF-α) in culture medium was determined using the corresponding ELISA kits against TNF-α and IL-1β (Beyotime) according to the user’s manual. ..

    LDH Cytotoxicity Assay:

    Article Title: Enhanced liver but not muscle OXPHOS in diabetes and reduced glucose output by complex I inhibition, et al. Enhanced liver but not muscle OXPHOS in diabetes and reduced glucose output by complex I inhibition
    Article Snippet: After the cells were treated for 6 hours, 50 μL culture medium was taken to measure the glucose concentration. .. LDH contents in the medium and cells were detected simultaneously using the culture medium with a LDH‐Cytotoxicity Assay Kit (Beyotime) as instructed. .. 2.13 Fatty acid treatment OA was dissolved in H2 O via being incubated at 75°C thermostatic water bath; then, OA was bound with fatty acid‐free BSA to gain 40 mmol/L OA (with 10% BSA) stock solution.

    Staining:

    Article Title: Similar Repair Effects of Human Placenta, Bone Marrow Mesenchymal Stem Cells, and Their Exosomes for Damaged SVOG Ovarian Granulosa Cells
    Article Snippet: Three parallel wells were set to each group, and the subsequent experiments were performed after the cells in each group were further cultured for 48 h in a DMEM/F-12 complete medium (Figures and ). .. Detection of Cell Senescence by β -Galactosidase Staining The cell culture medium in the 6-well culture plates was aspirated, and the cells were washed once with PBS, before 1 mL of β-galactosidase staining and fixation solution (Beyotime, Shanghai, China) was added to each well to fix the cells at room temperature for 15 min. ..

    Cell Culture:

    Article Title: Similar Repair Effects of Human Placenta, Bone Marrow Mesenchymal Stem Cells, and Their Exosomes for Damaged SVOG Ovarian Granulosa Cells
    Article Snippet: Three parallel wells were set to each group, and the subsequent experiments were performed after the cells in each group were further cultured for 48 h in a DMEM/F-12 complete medium (Figures and ). .. Detection of Cell Senescence by β -Galactosidase Staining The cell culture medium in the 6-well culture plates was aspirated, and the cells were washed once with PBS, before 1 mL of β-galactosidase staining and fixation solution (Beyotime, Shanghai, China) was added to each well to fix the cells at room temperature for 15 min. ..

    Article Title: Downregulation of circular RNA hsa_circ_0000735 boosts prostate cancer sensitivity to docetaxel via sponging miR-7
    Article Snippet: 18S ribosomal RNA (rRNA) or U6 snRNA were used as the internal reference for cytoplasm and nucleus, respectively. .. Cell Counting Kit-8 (CCK-8) assay For cytotoxic and half-maximal inhibitory concentration (IC50) value assessment, the transfected PC-3/DTX and DU145/DTX cells (2.0 × 103 ) were cultured in cell medium with different concentrations of DTX (5, 10, 20, 40, 80, or 160 nM) for 48 h. For cell viability analysis, the transfected PC-3/DTX and DU145/DTX cells (2.0 × 103) were seeded into 96-well plates with cell medium (10 nM DTX) for 24 h, 48 h, or 72 h. Thereafter, the CCK-8 solution (10 μL, Beyotime, Shanghai, China) was added to each well and incubated for 2 h. The absorbance at 450 nm was measured with a microplate reader (Bio-Rad, Hercules, CA, USA). .. Cell colony formation assay The transfected PC-3/DTX and DU145/DTX cells (1.0 × 102 cells/well) were seeded into 6-well plates and then treated with 10 nM DTX when the cell was attached to the wall.

    Article Title: Inhibition of miR-450b-5p ameliorates hepatic ischemia/reperfusion injury via targeting CRYAB
    Article Snippet: All cells were incubated using Dulbecco’s Modified Eagle Medium—Nutrient Mixture F-12 (Gibco, Grand Island, USA), with a 10% concentration of fetal bovine serum (Gibco, Australia). .. Cell culture medium contains 100 U/ml penicillin and 0.1 mg/ml streptomycin (Beyotime, Shanghai, China). .. Conventional cell incubator (Thermo, MA, USA) was set into 5% CO2 at the temperature of 37 °C.

    CCK-8 Assay:

    Article Title: Downregulation of circular RNA hsa_circ_0000735 boosts prostate cancer sensitivity to docetaxel via sponging miR-7
    Article Snippet: 18S ribosomal RNA (rRNA) or U6 snRNA were used as the internal reference for cytoplasm and nucleus, respectively. .. Cell Counting Kit-8 (CCK-8) assay For cytotoxic and half-maximal inhibitory concentration (IC50) value assessment, the transfected PC-3/DTX and DU145/DTX cells (2.0 × 103 ) were cultured in cell medium with different concentrations of DTX (5, 10, 20, 40, 80, or 160 nM) for 48 h. For cell viability analysis, the transfected PC-3/DTX and DU145/DTX cells (2.0 × 103) were seeded into 96-well plates with cell medium (10 nM DTX) for 24 h, 48 h, or 72 h. Thereafter, the CCK-8 solution (10 μL, Beyotime, Shanghai, China) was added to each well and incubated for 2 h. The absorbance at 450 nm was measured with a microplate reader (Bio-Rad, Hercules, CA, USA). .. Cell colony formation assay The transfected PC-3/DTX and DU145/DTX cells (1.0 × 102 cells/well) were seeded into 6-well plates and then treated with 10 nM DTX when the cell was attached to the wall.

    Concentration Assay:

    Article Title: Downregulation of circular RNA hsa_circ_0000735 boosts prostate cancer sensitivity to docetaxel via sponging miR-7
    Article Snippet: 18S ribosomal RNA (rRNA) or U6 snRNA were used as the internal reference for cytoplasm and nucleus, respectively. .. Cell Counting Kit-8 (CCK-8) assay For cytotoxic and half-maximal inhibitory concentration (IC50) value assessment, the transfected PC-3/DTX and DU145/DTX cells (2.0 × 103 ) were cultured in cell medium with different concentrations of DTX (5, 10, 20, 40, 80, or 160 nM) for 48 h. For cell viability analysis, the transfected PC-3/DTX and DU145/DTX cells (2.0 × 103) were seeded into 96-well plates with cell medium (10 nM DTX) for 24 h, 48 h, or 72 h. Thereafter, the CCK-8 solution (10 μL, Beyotime, Shanghai, China) was added to each well and incubated for 2 h. The absorbance at 450 nm was measured with a microplate reader (Bio-Rad, Hercules, CA, USA). .. Cell colony formation assay The transfected PC-3/DTX and DU145/DTX cells (1.0 × 102 cells/well) were seeded into 6-well plates and then treated with 10 nM DTX when the cell was attached to the wall.

    Incubation:

    Article Title: Downregulation of circular RNA hsa_circ_0000735 boosts prostate cancer sensitivity to docetaxel via sponging miR-7
    Article Snippet: 18S ribosomal RNA (rRNA) or U6 snRNA were used as the internal reference for cytoplasm and nucleus, respectively. .. Cell Counting Kit-8 (CCK-8) assay For cytotoxic and half-maximal inhibitory concentration (IC50) value assessment, the transfected PC-3/DTX and DU145/DTX cells (2.0 × 103 ) were cultured in cell medium with different concentrations of DTX (5, 10, 20, 40, 80, or 160 nM) for 48 h. For cell viability analysis, the transfected PC-3/DTX and DU145/DTX cells (2.0 × 103) were seeded into 96-well plates with cell medium (10 nM DTX) for 24 h, 48 h, or 72 h. Thereafter, the CCK-8 solution (10 μL, Beyotime, Shanghai, China) was added to each well and incubated for 2 h. The absorbance at 450 nm was measured with a microplate reader (Bio-Rad, Hercules, CA, USA). .. Cell colony formation assay The transfected PC-3/DTX and DU145/DTX cells (1.0 × 102 cells/well) were seeded into 6-well plates and then treated with 10 nM DTX when the cell was attached to the wall.

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    Beyotime pbs medium
    Percentage of BrdU positive eosinophils of <t>BALF,</t> blood and bone marrow from wild type and IL-25 KO mouse models that were sensitized and challenged with OVA or <t>PBS</t> (control) (*p
    Pbs Medium, supplied by Beyotime, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs medium/product/Beyotime
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbs medium - by Bioz Stars, 2021-04
    97/100 stars
      Buy from Supplier

    Image Search Results


    Percentage of BrdU positive eosinophils of BALF, blood and bone marrow from wild type and IL-25 KO mouse models that were sensitized and challenged with OVA or PBS (control) (*p

    Journal: Clinical and Translational Allergy

    Article Title: Interleukin-25 and eosinophils progenitor cell mobilization in allergic asthma

    doi: 10.1186/s13601-018-0190-2

    Figure Lengend Snippet: Percentage of BrdU positive eosinophils of BALF, blood and bone marrow from wild type and IL-25 KO mouse models that were sensitized and challenged with OVA or PBS (control) (*p

    Article Snippet: Measurement of eosinophil number in BALF Cells were seeded in PBS medium (Beyotime Institute of Biotechnology, Haimen, China) at 1 × 105 cells/mL and stained with Fast Wright and Giemsa Stain kit (Nanjing Jiancheng Technology Co., Ltd., Nanjing, China), according to manufacturer’s protocol.

    Techniques:

    Eosinophil percentage of BALF, blood and bone marrow from wild type and IL-25 KO mouse models that were sensitized and challenged with OVA or PBS (control) (*p

    Journal: Clinical and Translational Allergy

    Article Title: Interleukin-25 and eosinophils progenitor cell mobilization in allergic asthma

    doi: 10.1186/s13601-018-0190-2

    Figure Lengend Snippet: Eosinophil percentage of BALF, blood and bone marrow from wild type and IL-25 KO mouse models that were sensitized and challenged with OVA or PBS (control) (*p

    Article Snippet: Measurement of eosinophil number in BALF Cells were seeded in PBS medium (Beyotime Institute of Biotechnology, Haimen, China) at 1 × 105 cells/mL and stained with Fast Wright and Giemsa Stain kit (Nanjing Jiancheng Technology Co., Ltd., Nanjing, China), according to manufacturer’s protocol.

    Techniques: