pbs containing protease inhibitor cocktail  (Roche)

 
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    Roche pbs containing protease inhibitor cocktail
    ST398 CA-MRSA virulence in a mouse model. The seven ST398 CA-MRSA isolates were compared with closely related ST398 MSSA, all ST398 LA-MSSA obtained for this study, and representative Chinese ST5 and ST239 HA-MRSA strains by in vivo analysis of virulence. In addition, two USA300 CA-MRSA strains (SF8300, LAC) and the genome-sequenced LA-MRSA S0385 standard strain were used in the comparisons. Isolate selection for the MSSA and HA-MRSA groups was performed, as in a previous study [ 26 ], by selecting isolates whose average PSM production was similar to that of the entire group and thus reflects the average virulence potential. Notably, for the in vivo infection studies, the ST398 CA-MRSA, MSSA, and ST5/ST239 HA-MRSA isolates were obtained from the corresponding type of human infection (respiratory and skin infection, respectively). See Additional file 1 : Table S2 for isolates that were compared and their characteristics. a Mouse skin infection model. Mice received 10 7 live S. aureus or <t>PBS</t> alone in the right flank by subcutaneous injection. Skin abscesses (length × width) were measured 48 h after infection. b Mouse lung infection model. We pipetted 4 × 10 9 CFU/40 μl S. aureus into the nares of the anesthetized mice. All mice were euthanized 48 h after inoculation and CFU in the lung tissue were determined. a , b Three mice were infected with each strain; the shown symbols represent the average value obtained from three mice. c – f Inflammatory <t>cytokine</t> expression in ST398 CA-MRSA isolates in comparison. The inflammatory cytokines TNF-α and IL-6 were determined in skin ( c , d ) or lung tissue ( e , f ). a – f Statistical analysis is by one-way ANOVA with Dunnett’s post test versus the ST398 CA-MRSA group. * p
    Pbs Containing Protease Inhibitor Cocktail, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Detection and analysis of methicillin-resistant human-adapted sequence type 398 allows insight into community-associated methicillin-resistant Staphylococcus aureus evolution"

    Article Title: Detection and analysis of methicillin-resistant human-adapted sequence type 398 allows insight into community-associated methicillin-resistant Staphylococcus aureus evolution

    Journal: Genome Medicine

    doi: 10.1186/s13073-018-0514-9

    ST398 CA-MRSA virulence in a mouse model. The seven ST398 CA-MRSA isolates were compared with closely related ST398 MSSA, all ST398 LA-MSSA obtained for this study, and representative Chinese ST5 and ST239 HA-MRSA strains by in vivo analysis of virulence. In addition, two USA300 CA-MRSA strains (SF8300, LAC) and the genome-sequenced LA-MRSA S0385 standard strain were used in the comparisons. Isolate selection for the MSSA and HA-MRSA groups was performed, as in a previous study [ 26 ], by selecting isolates whose average PSM production was similar to that of the entire group and thus reflects the average virulence potential. Notably, for the in vivo infection studies, the ST398 CA-MRSA, MSSA, and ST5/ST239 HA-MRSA isolates were obtained from the corresponding type of human infection (respiratory and skin infection, respectively). See Additional file 1 : Table S2 for isolates that were compared and their characteristics. a Mouse skin infection model. Mice received 10 7 live S. aureus or PBS alone in the right flank by subcutaneous injection. Skin abscesses (length × width) were measured 48 h after infection. b Mouse lung infection model. We pipetted 4 × 10 9 CFU/40 μl S. aureus into the nares of the anesthetized mice. All mice were euthanized 48 h after inoculation and CFU in the lung tissue were determined. a , b Three mice were infected with each strain; the shown symbols represent the average value obtained from three mice. c – f Inflammatory cytokine expression in ST398 CA-MRSA isolates in comparison. The inflammatory cytokines TNF-α and IL-6 were determined in skin ( c , d ) or lung tissue ( e , f ). a – f Statistical analysis is by one-way ANOVA with Dunnett’s post test versus the ST398 CA-MRSA group. * p
    Figure Legend Snippet: ST398 CA-MRSA virulence in a mouse model. The seven ST398 CA-MRSA isolates were compared with closely related ST398 MSSA, all ST398 LA-MSSA obtained for this study, and representative Chinese ST5 and ST239 HA-MRSA strains by in vivo analysis of virulence. In addition, two USA300 CA-MRSA strains (SF8300, LAC) and the genome-sequenced LA-MRSA S0385 standard strain were used in the comparisons. Isolate selection for the MSSA and HA-MRSA groups was performed, as in a previous study [ 26 ], by selecting isolates whose average PSM production was similar to that of the entire group and thus reflects the average virulence potential. Notably, for the in vivo infection studies, the ST398 CA-MRSA, MSSA, and ST5/ST239 HA-MRSA isolates were obtained from the corresponding type of human infection (respiratory and skin infection, respectively). See Additional file 1 : Table S2 for isolates that were compared and their characteristics. a Mouse skin infection model. Mice received 10 7 live S. aureus or PBS alone in the right flank by subcutaneous injection. Skin abscesses (length × width) were measured 48 h after infection. b Mouse lung infection model. We pipetted 4 × 10 9 CFU/40 μl S. aureus into the nares of the anesthetized mice. All mice were euthanized 48 h after inoculation and CFU in the lung tissue were determined. a , b Three mice were infected with each strain; the shown symbols represent the average value obtained from three mice. c – f Inflammatory cytokine expression in ST398 CA-MRSA isolates in comparison. The inflammatory cytokines TNF-α and IL-6 were determined in skin ( c , d ) or lung tissue ( e , f ). a – f Statistical analysis is by one-way ANOVA with Dunnett’s post test versus the ST398 CA-MRSA group. * p

    Techniques Used: In Vivo, Selection, Infection, Mouse Assay, Injection, Expressing

    2) Product Images from "Detection and analysis of methicillin-resistant human-adapted sequence type 398 allows insight into community-associated methicillin-resistant Staphylococcus aureus evolution"

    Article Title: Detection and analysis of methicillin-resistant human-adapted sequence type 398 allows insight into community-associated methicillin-resistant Staphylococcus aureus evolution

    Journal: Genome Medicine

    doi: 10.1186/s13073-018-0514-9

    : Table S2 for isolates that were compared and their characteristics. a Mouse skin infection model. Mice received 10 7 live S. aureus or PBS alone in the right flank by subcutaneous injection. Skin abscesses (length × width) were measured 48 h after infection. b Mouse lung infection model. We pipetted 4 × 10 9 CFU/40 μl S. aureus into the nares of the anesthetized mice. All mice were euthanized 48 h after inoculation and CFU in the lung tissue were determined. a , b Three mice were infected with each strain; the shown symbols represent the average value obtained from three mice. c – f Inflammatory cytokine expression in ST398 CA-MRSA isolates in comparison. The inflammatory cytokines TNF-α and IL-6 were determined in skin ( c , d ) or lung tissue ( e , f ). a – f Statistical analysis is by one-way ANOVA with Dunnett’s post test versus the ST398 CA-MRSA group. * p
    Figure Legend Snippet: : Table S2 for isolates that were compared and their characteristics. a Mouse skin infection model. Mice received 10 7 live S. aureus or PBS alone in the right flank by subcutaneous injection. Skin abscesses (length × width) were measured 48 h after infection. b Mouse lung infection model. We pipetted 4 × 10 9 CFU/40 μl S. aureus into the nares of the anesthetized mice. All mice were euthanized 48 h after inoculation and CFU in the lung tissue were determined. a , b Three mice were infected with each strain; the shown symbols represent the average value obtained from three mice. c – f Inflammatory cytokine expression in ST398 CA-MRSA isolates in comparison. The inflammatory cytokines TNF-α and IL-6 were determined in skin ( c , d ) or lung tissue ( e , f ). a – f Statistical analysis is by one-way ANOVA with Dunnett’s post test versus the ST398 CA-MRSA group. * p

    Techniques Used: Infection, Mouse Assay, Injection, Expressing

    Related Articles

    Isolation:

    Article Title: Endogenous Parkin Preserves Dopaminergic Substantia Nigral Neurons following Mitochondrial DNA Mutagenic Stress
    Article Snippet: .. Homogenates were prepared from isolated striatum homogenized with a hand-held rotor (VWR) in PBS-containing protease inhibitor cocktail (Roche). ..

    Infection:

    Article Title: Detection and analysis of methicillin-resistant human-adapted sequence type 398 allows insight into community-associated methicillin-resistant Staphylococcus aureus evolution
    Article Snippet: Paragraph title: Mouse skin abscess and lung infection models ... Length (L) and width (W) values were used to calculate the area of lesions with the formula L × W. Mouse skin tissue of the same size was taken from the abscess and PBS control groups and homogenated with glass beads with PBS-containing protease inhibitor cocktail (Roche) for cytokine detection.

    Purification:

    Article Title: A protein complex containing Epo1p anchors the cortical endoplasmic reticulum to the yeast bud tip
    Article Snippet: Cells were pelleted, washed once with PBS, resuspended in PBS-containing protease inhibitor cocktail (Roche), lysed by lysozyme treatment (1 mg/ml; 30 min on ice), and subjected to sonication with a Bandelin Sonopuls HD 2070 (Reichmann Industrieservice). .. MBP fusion proteins were purified by immobilization on amylose beads for 1 h at 4°C followed by elution with 0.1 ml buffer containing 20 mM Tris-Cl, 0.2 M NaCl, 1 mM EDTA, and 10 mM maltose.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Detection and analysis of methicillin-resistant human-adapted sequence type 398 allows insight into community-associated methicillin-resistant Staphylococcus aureus evolution
    Article Snippet: Length (L) and width (W) values were used to calculate the area of lesions with the formula L × W. Mouse skin tissue of the same size was taken from the abscess and PBS control groups and homogenated with glass beads with PBS-containing protease inhibitor cocktail (Roche) for cytokine detection. .. Quantikine ELISA mouse cytokine detection kits (R & D Systems, Minneapolis, MN, USA) were used to concentrate mouse tumor necrosis factor (TNF)-α and interleukin (IL)-6 from the skin homogenate.

    Activity Assay:

    Article Title: Endogenous Parkin Preserves Dopaminergic Substantia Nigral Neurons following Mitochondrial DNA Mutagenic Stress
    Article Snippet: Complex I, IV, and citrate synthase activity were measured as previously described ( ). .. Homogenates were prepared from isolated striatum homogenized with a hand-held rotor (VWR) in PBS-containing protease inhibitor cocktail (Roche).

    Expressing:

    Article Title: A protein complex containing Epo1p anchors the cortical endoplasmic reticulum to the yeast bud tip
    Article Snippet: Paragraph title: Expression and preparation of fusion proteins in E. coli ... Cells were pelleted, washed once with PBS, resuspended in PBS-containing protease inhibitor cocktail (Roche), lysed by lysozyme treatment (1 mg/ml; 30 min on ice), and subjected to sonication with a Bandelin Sonopuls HD 2070 (Reichmann Industrieservice).

    Protease Inhibitor:

    Article Title: A protein complex containing Epo1p anchors the cortical endoplasmic reticulum to the yeast bud tip
    Article Snippet: .. Cells were pelleted, washed once with PBS, resuspended in PBS-containing protease inhibitor cocktail (Roche), lysed by lysozyme treatment (1 mg/ml; 30 min on ice), and subjected to sonication with a Bandelin Sonopuls HD 2070 (Reichmann Industrieservice). .. MBP fusion proteins were purified by immobilization on amylose beads for 1 h at 4°C followed by elution with 0.1 ml buffer containing 20 mM Tris-Cl, 0.2 M NaCl, 1 mM EDTA, and 10 mM maltose.

    Article Title: Endogenous Parkin Preserves Dopaminergic Substantia Nigral Neurons following Mitochondrial DNA Mutagenic Stress
    Article Snippet: .. Homogenates were prepared from isolated striatum homogenized with a hand-held rotor (VWR) in PBS-containing protease inhibitor cocktail (Roche). ..

    Article Title: Detection and analysis of methicillin-resistant human-adapted sequence type 398 allows insight into community-associated methicillin-resistant Staphylococcus aureus evolution
    Article Snippet: .. Length (L) and width (W) values were used to calculate the area of lesions with the formula L × W. Mouse skin tissue of the same size was taken from the abscess and PBS control groups and homogenated with glass beads with PBS-containing protease inhibitor cocktail (Roche) for cytokine detection. .. Quantikine ELISA mouse cytokine detection kits (R & D Systems, Minneapolis, MN, USA) were used to concentrate mouse tumor necrosis factor (TNF)-α and interleukin (IL)-6 from the skin homogenate.

    Sonication:

    Article Title: A protein complex containing Epo1p anchors the cortical endoplasmic reticulum to the yeast bud tip
    Article Snippet: .. Cells were pelleted, washed once with PBS, resuspended in PBS-containing protease inhibitor cocktail (Roche), lysed by lysozyme treatment (1 mg/ml; 30 min on ice), and subjected to sonication with a Bandelin Sonopuls HD 2070 (Reichmann Industrieservice). .. MBP fusion proteins were purified by immobilization on amylose beads for 1 h at 4°C followed by elution with 0.1 ml buffer containing 20 mM Tris-Cl, 0.2 M NaCl, 1 mM EDTA, and 10 mM maltose.

    Injection:

    Article Title: Detection and analysis of methicillin-resistant human-adapted sequence type 398 allows insight into community-associated methicillin-resistant Staphylococcus aureus evolution
    Article Snippet: For the abscess model, mice were anesthetized with isoflurane and inoculated with 50 μl PBS containing 107 live S. aureus or PBS alone in the right flank by subcutaneous injection. .. Length (L) and width (W) values were used to calculate the area of lesions with the formula L × W. Mouse skin tissue of the same size was taken from the abscess and PBS control groups and homogenated with glass beads with PBS-containing protease inhibitor cocktail (Roche) for cytokine detection.

    Mouse Assay:

    Article Title: Detection and analysis of methicillin-resistant human-adapted sequence type 398 allows insight into community-associated methicillin-resistant Staphylococcus aureus evolution
    Article Snippet: For the abscess model, mice were anesthetized with isoflurane and inoculated with 50 μl PBS containing 107 live S. aureus or PBS alone in the right flank by subcutaneous injection. .. Length (L) and width (W) values were used to calculate the area of lesions with the formula L × W. Mouse skin tissue of the same size was taken from the abscess and PBS control groups and homogenated with glass beads with PBS-containing protease inhibitor cocktail (Roche) for cytokine detection.

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    Roche cold pbs wash
    Cold Pbs Wash, supplied by Roche, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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