pbs cacl2  (Millipore)


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    Name:
    Phosphate Buffered Saline
    Description:
    Phosphate Buffered Saline PBS is routinely used as a wash buffer in Western blot and immunoprecipitation IP procedures as well as during sample preparation of cell lysates for use in general molecular biology applications
    Catalog Number:
    p7059
    Price:
    None
    Applications:
    Suitable for use in wash buffers for Western blots and IP, as well as cell lysate sample preparation.
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    Structured Review

    Millipore pbs cacl2
    Phosphate Buffered Saline PBS is routinely used as a wash buffer in Western blot and immunoprecipitation IP procedures as well as during sample preparation of cell lysates for use in general molecular biology applications
    https://www.bioz.com/result/pbs cacl2/product/Millipore
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    pbs cacl2 - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Transfection:

    Article Title: SIRT1 ameliorates oxidative stress induced neural cell death and is down-regulated in Parkinson’s disease
    Article Snippet: .. Transfected cells were treated with either diquat (Sigma-Aldrich, UK) dissolved in PBS (phosphate buffered saline; Sigma-Aldrich) or rotenone (Sigma-Aldrich) dissolved in DMSO (dimethyl sulphoxide, Sigma-Aldrich) at a final concentration of 0.2% PBS/DMSO and incubated overnight for 20 h. Cell viability was determined by Alamar Blue reduction assay [ ]. .. Western blotting Following Alamar Blue fluorescence, cell lysates were prepared by scraping the viable cells in native lysis buffer (1% 10× tris buffered saline (TBS), 0.27 M Sucrose, 1% Triton X-100, 1× protease inhibitor cocktail).

    Concentration Assay:

    Article Title: SIRT1 ameliorates oxidative stress induced neural cell death and is down-regulated in Parkinson’s disease
    Article Snippet: .. Transfected cells were treated with either diquat (Sigma-Aldrich, UK) dissolved in PBS (phosphate buffered saline; Sigma-Aldrich) or rotenone (Sigma-Aldrich) dissolved in DMSO (dimethyl sulphoxide, Sigma-Aldrich) at a final concentration of 0.2% PBS/DMSO and incubated overnight for 20 h. Cell viability was determined by Alamar Blue reduction assay [ ]. .. Western blotting Following Alamar Blue fluorescence, cell lysates were prepared by scraping the viable cells in native lysis buffer (1% 10× tris buffered saline (TBS), 0.27 M Sucrose, 1% Triton X-100, 1× protease inhibitor cocktail).

    Cytometry:

    Article Title: The ability of antigen, but not interleukin-2, to promote n-butyrate-induced T helper 1 cell anergy is associated with increased expression and altered association patterns of cyclin-dependent kinase inhibitors
    Article Snippet: .. The fixed Th1 cells were then washed in phosphate-buffered saline (PBS), resuspended in 1 ml of PBS containing RNAse (1 mg/ml; Sigma) and propidium iodide (50 µg/ml; Sigma), incubated for 20 min in the dark at room temperature and analysed by flow cytometry using a FACSCalibur (Becton-Dickinson, Mountain View, CA). .. The data was analysed using modfit (Verity Software House, Topsham, ME).

    Labeling:

    Article Title: Omental Tissue-Mediated Tumorigenesis of Gastric Cancer Peritoneal Metastases
    Article Snippet: .. Exosomes were diluted in PBS and labeled with PKH-67 green fluorescent cell linker cell membrane labeling dye (4 μl) (Sigma-Aldrich) was added to 1 ml of Diluent C before being added to the exosomes. ..

    Activation Assay:

    Article Title: MFG-E8-derived Peptide MSP68 is a Cytoskeletal Immunomodulator of Neutrophils that Inhibits Rac1
    Article Snippet: .. BMDNs were pre-treated with either PBS as vehicle or MSP68 at a dose of 10 mM for 30 min, followed by the stimulation with the N-formylmethionine-leucine-phenylalanine ( f -MLP) (Tocris BioSci, Bristol, UK ) at 10 mM for 5 min. After activation with f -MLP, cells were fixed with 3.7% methanol free-formaldehyde in PBS (Sigma, St. Louis, MO) for 10 min at room temperature. .. Slides were then spun at 100 g for 5 min and supernatants were aspirated with fine glass pipette tip.

    Incubation:

    Article Title: SIRT1 ameliorates oxidative stress induced neural cell death and is down-regulated in Parkinson’s disease
    Article Snippet: .. Transfected cells were treated with either diquat (Sigma-Aldrich, UK) dissolved in PBS (phosphate buffered saline; Sigma-Aldrich) or rotenone (Sigma-Aldrich) dissolved in DMSO (dimethyl sulphoxide, Sigma-Aldrich) at a final concentration of 0.2% PBS/DMSO and incubated overnight for 20 h. Cell viability was determined by Alamar Blue reduction assay [ ]. .. Western blotting Following Alamar Blue fluorescence, cell lysates were prepared by scraping the viable cells in native lysis buffer (1% 10× tris buffered saline (TBS), 0.27 M Sucrose, 1% Triton X-100, 1× protease inhibitor cocktail).

    Article Title: The ability of antigen, but not interleukin-2, to promote n-butyrate-induced T helper 1 cell anergy is associated with increased expression and altered association patterns of cyclin-dependent kinase inhibitors
    Article Snippet: .. The fixed Th1 cells were then washed in phosphate-buffered saline (PBS), resuspended in 1 ml of PBS containing RNAse (1 mg/ml; Sigma) and propidium iodide (50 µg/ml; Sigma), incubated for 20 min in the dark at room temperature and analysed by flow cytometry using a FACSCalibur (Becton-Dickinson, Mountain View, CA). .. The data was analysed using modfit (Verity Software House, Topsham, ME).

    Article Title: Generation and Immunogenicity of a Recombinant Pseudorabies Virus Co-Expressing Classical Swine Fever Virus E2 Protein and Porcine Circovirus Type 2 Capsid Protein Based on Fosmid Library Platform
    Article Snippet: .. The cells were then air-dried and incubated with 100 µL (1:300 in PBS) of anti-E2 monoclonal antibody (MAb) HQ06 [ ] and anti-Cap MAb 36A9 (Ingenasa, Madrid, Spain) [ ] for 2 h at 37°C, subsequently washed thrice with phosphate-buffered saline (PBS), and incubated with fluorescein isothiocyanate (FITC)-conjugated anti-mouse IgG (Sigma-Aldrich, USA) for 1 h at 37°C. .. After washing the cells three times with PBS, the plates were visualized under a fluorescence microscope (Nikon TE200; Japan).

    Recombinant:

    Article Title: Alpha Interferon Induces Long-Lasting Refractoriness of JAK-STAT Signaling in the Mouse Liver through Induction of USP18/UBP43
    Article Snippet: .. The s.c. injections with phosphate-buffered saline (PBS) or mIFN-α were performed between 8:00 a.m. and 5:00 p.m. Recombinant mIFN-α was purchased from CalBiochem (Juro Supply GmbH, Switzerland). ..

    Article Title: Assessing single-stranded oligonucleotide drug-induced effects in vitro reveals key risk factors for thrombocytopenia
    Article Snippet: .. Briefly, microtiter plates (Maxisorp 96well, Nunc #442404) were coated with human recombinant PF4 (10 μg/ml, ProSpec-Tany TechnoGene, Rehovot, Israel) in phosphate buffered saline (PBS) for 2h followed by the addition of either vehicle, heparin (0.002–200 μg/ml, Sigma-Aldrich, Darmstadt, Germany) or ONs (0.001–3 μM). .. After an overnight incubation, the plates were washed 3x with 0.1% Tween-20 and blocked with 3% BSA in PBS for 2 h. After washing with 0.1% Tween-20 plates were incubated with KKO antibody (0.1 μg/ml in 0.005% Tween 20, ThermoFisher Scientific, Waltham, MA, USA) for 1h.

    Flow Cytometry:

    Article Title: The ability of antigen, but not interleukin-2, to promote n-butyrate-induced T helper 1 cell anergy is associated with increased expression and altered association patterns of cyclin-dependent kinase inhibitors
    Article Snippet: .. The fixed Th1 cells were then washed in phosphate-buffered saline (PBS), resuspended in 1 ml of PBS containing RNAse (1 mg/ml; Sigma) and propidium iodide (50 µg/ml; Sigma), incubated for 20 min in the dark at room temperature and analysed by flow cytometry using a FACSCalibur (Becton-Dickinson, Mountain View, CA). .. The data was analysed using modfit (Verity Software House, Topsham, ME).

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