Structured Review

Addgene inc pbmn megfp c1
Pbmn Megfp C1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbmn megfp c1/product/Addgene inc
Average 79 stars, based on 1 article reviews
Price from $9.99 to $1999.99
pbmn megfp c1 - by Bioz Stars, 2020-01
79/100 stars

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Related Articles

Clone Assay:

Article Title: The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy
Article Snippet: pMXs-puro-GFP-WIPI1 and pMXs-puro-GFP-DFCP1 were a kind gift from Dr. N. Mizushima (University of Toyko, Japan) and pMXs-IP-GFP-ULK1 was purchased from Addgene (#38193). .. To generate pBMN-mEGFP-C1, mEGFP-C1 (Addgene #36412) was PCR amplified (together with the multiple cloning site) and cloned into pBMN-Z at BamHI/SalI sites using the Gibson Cloning kit (New England BioLabs) according to manufacturer's instructions. .. The BamHI and SalI sites used to insert mEGFP-C1 were not regenerated.

Amplification:

Article Title: The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy
Article Snippet: pMXs-puro-GFP-WIPI1 and pMXs-puro-GFP-DFCP1 were a kind gift from Dr. N. Mizushima (University of Toyko, Japan) and pMXs-IP-GFP-ULK1 was purchased from Addgene (#38193). .. To generate pBMN-mEGFP-C1, mEGFP-C1 (Addgene #36412) was PCR amplified (together with the multiple cloning site) and cloned into pBMN-Z at BamHI/SalI sites using the Gibson Cloning kit (New England BioLabs) according to manufacturer's instructions. .. The BamHI and SalI sites used to insert mEGFP-C1 were not regenerated.

Stable Transfection:

Article Title: The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy
Article Snippet: Paragraph title: Cloning and generation of stable cell lines ... To generate pBMN-mEGFP-C1, mEGFP-C1 (Addgene #36412) was PCR amplified (together with the multiple cloning site) and cloned into pBMN-Z at BamHI/SalI sites using the Gibson Cloning kit (New England BioLabs) according to manufacturer's instructions.

Ligation:

Article Title: The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy
Article Snippet: To generate pBMN-mEGFP-C1, mEGFP-C1 (Addgene #36412) was PCR amplified (together with the multiple cloning site) and cloned into pBMN-Z at BamHI/SalI sites using the Gibson Cloning kit (New England BioLabs) according to manufacturer's instructions. .. To generate pBMN-mEGFP-C1, mEGFP-C1 (Addgene #36412) was PCR amplified (together with the multiple cloning site) and cloned into pBMN-Z at BamHI/SalI sites using the Gibson Cloning kit (New England BioLabs) according to manufacturer's instructions.

Generated:

Article Title: The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy
Article Snippet: To generate pBMN-mEGFP-C1, mEGFP-C1 (Addgene #36412) was PCR amplified (together with the multiple cloning site) and cloned into pBMN-Z at BamHI/SalI sites using the Gibson Cloning kit (New England BioLabs) according to manufacturer's instructions. .. To generate pBMN-mEGFP-C1, mEGFP-C1 (Addgene #36412) was PCR amplified (together with the multiple cloning site) and cloned into pBMN-Z at BamHI/SalI sites using the Gibson Cloning kit (New England BioLabs) according to manufacturer's instructions.

Polymerase Chain Reaction:

Article Title: The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy
Article Snippet: pMXs-puro-GFP-WIPI1 and pMXs-puro-GFP-DFCP1 were a kind gift from Dr. N. Mizushima (University of Toyko, Japan) and pMXs-IP-GFP-ULK1 was purchased from Addgene (#38193). .. To generate pBMN-mEGFP-C1, mEGFP-C1 (Addgene #36412) was PCR amplified (together with the multiple cloning site) and cloned into pBMN-Z at BamHI/SalI sites using the Gibson Cloning kit (New England BioLabs) according to manufacturer's instructions. .. The BamHI and SalI sites used to insert mEGFP-C1 were not regenerated.

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    Addgene inc pbmn megfp c1
    Pbmn Megfp C1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbmn megfp c1/product/Addgene inc
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbmn megfp c1 - by Bioz Stars, 2020-01
    79/100 stars
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