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EphA3 co-stains with macrophage/leukocyte markers A. Immunofluorescent staining of CD68, GFAP and Von Willebrand factor in a GBM specimen. B. Immunofluorescent staining of EphA3 (red) and CD31, GFAP, CD68, CD163, and CD206 on consecutive frozen sections of BTCOE4443 human GBM specimen. Nuclei are stained with DAPI (blue). Selected areas were magnified (last column on the right). C. Confocal immunofluorescent staining of EphA3 and CD68 in a GBM specimen using stacked 2D images. D, E. -stacked 2D images, and F. Confocal microscopy of co-staining of EphA3 and CD45 in GBM specimens. G. <t>CD4+</t> T cells do not express the EphA3 receptor. Western blot analysis of peripheral blood mononuclear cells <t>(PBMC),</t> CD4+ and CD4+ activated T cells were probed for EphA3 immunoreactivity.
Pbmc, supplied by ZenBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Simultaneous targeting of Eph receptors in glioblastoma"

Article Title: Simultaneous targeting of Eph receptors in glioblastoma

Journal: Oncotarget

doi: 10.18632/oncotarget.10978

EphA3 co-stains with macrophage/leukocyte markers A. Immunofluorescent staining of CD68, GFAP and Von Willebrand factor in a GBM specimen. B. Immunofluorescent staining of EphA3 (red) and CD31, GFAP, CD68, CD163, and CD206 on consecutive frozen sections of BTCOE4443 human GBM specimen. Nuclei are stained with DAPI (blue). Selected areas were magnified (last column on the right). C. Confocal immunofluorescent staining of EphA3 and CD68 in a GBM specimen using stacked 2D images. D, E. -stacked 2D images, and F. Confocal microscopy of co-staining of EphA3 and CD45 in GBM specimens. G. CD4+ T cells do not express the EphA3 receptor. Western blot analysis of peripheral blood mononuclear cells (PBMC), CD4+ and CD4+ activated T cells were probed for EphA3 immunoreactivity.
Figure Legend Snippet: EphA3 co-stains with macrophage/leukocyte markers A. Immunofluorescent staining of CD68, GFAP and Von Willebrand factor in a GBM specimen. B. Immunofluorescent staining of EphA3 (red) and CD31, GFAP, CD68, CD163, and CD206 on consecutive frozen sections of BTCOE4443 human GBM specimen. Nuclei are stained with DAPI (blue). Selected areas were magnified (last column on the right). C. Confocal immunofluorescent staining of EphA3 and CD68 in a GBM specimen using stacked 2D images. D, E. -stacked 2D images, and F. Confocal microscopy of co-staining of EphA3 and CD45 in GBM specimens. G. CD4+ T cells do not express the EphA3 receptor. Western blot analysis of peripheral blood mononuclear cells (PBMC), CD4+ and CD4+ activated T cells were probed for EphA3 immunoreactivity.

Techniques Used: Staining, Confocal Microscopy, Western Blot

Related Articles

Isolation:

Article Title: Simultaneous targeting of Eph receptors in glioblastoma
Article Snippet: .. Isolation and activation of CD4+ T cells Total CD4+ T Cells were isolated from PBMC (ZenBio, Durham, NC) using the Dynabeads Untouched human CD4+ T-Cell Kit (Invitrogen) according to the manufacturer's instructions. .. The cells were plates at a density of 4 × 104 /ml in RPMI-1640 containing 10% FBS, 0.05 mM 2-mercaptoethanol and 30U/ml IL-2 (Peprotech, Rocky Hill, NJ).

Article Title: HIV-1 Nef counteracts autophagy restriction by enhancing the association between BECN1 and its inhibitor BCL2 in a PRKN-dependent manner.
Article Snippet: .. One million naïve CD4+ T cells isolated from PBMCs (Zenbio, Inc., SERPBCD4 TH-N-F) were activated using Dynabeads coated with anti-CD3 and anti-CD28 antibodies (ThermoFisher Scientific, 111.31D). .. Cells were also expanded using IL2 (interleukin 2; 30 IU/mL; NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH; 136) for 3 d. Next, cells were infected with 100 ng of p24 equivalents of HIV-1 NL4-3 or NL4-3 Δnef for 3 h at 37°C.

Cell Culture:

Article Title: Lactobacillus fermentum species ameliorate dextran sulfate sodium-induced colitis by regulating the immune response and altering gut microbiota
Article Snippet: .. PBMCs from healthy donors (ZenBio, Inc., Research Triangle Park, NC, USA) were cultured in RPMI 1640 medium (Gibco, Paisley, UK) containing 1% penicillin/streptomycin (Gibco), 1% gentamycin (Gibco), and 10% fetal bovine serum (Gibco). .. The viable cultured PBMCs were evaluated by trypan blue staining and counted using the CKX31 inverted microscope (Olympus Corp., Tokyo, Japan).

Activation Assay:

Article Title: Simultaneous targeting of Eph receptors in glioblastoma
Article Snippet: .. Isolation and activation of CD4+ T cells Total CD4+ T Cells were isolated from PBMC (ZenBio, Durham, NC) using the Dynabeads Untouched human CD4+ T-Cell Kit (Invitrogen) according to the manufacturer's instructions. .. The cells were plates at a density of 4 × 104 /ml in RPMI-1640 containing 10% FBS, 0.05 mM 2-mercaptoethanol and 30U/ml IL-2 (Peprotech, Rocky Hill, NJ).

other:

Article Title: Inactivation of the miR-183/96/182 Cluster Decreases the Severity of Pseudomonas aeruginosa-Induced Keratitis
Article Snippet: Human PMNs and PBMCs were purchased from Zen-Bio, Inc. (Research Triangle Park, NC, USA).

Reverse Transcription Polymerase Chain Reaction:

Article Title: Galectin-9 controls the therapeutic activity of 4-1BB–targeting antibodies
Article Snippet: .. For human 4-1BB.Fc, 4-1BB cDNA was obtained by RT-PCR using total RNA of activated PBMCs (cryopreserved from Zen-Bio). cDNA for full-length extracellular 4-1BB (CRD1-4 and transmembrane regions, amino acids 24–186) and each truncated form of 4-1BB (CRD1–3, 24–118; and CRD4 and transmembrane, 119–186) was ligated into the PS1121 huIgG1 Fc vector. .. For 4-1BBL.Fc, mouse 4-1BBL cDNA was obtained from total RNA of LPS-activated splenic CD11c+ DCs by RT-PCR.

Plasmid Preparation:

Article Title: Galectin-9 controls the therapeutic activity of 4-1BB–targeting antibodies
Article Snippet: .. For human 4-1BB.Fc, 4-1BB cDNA was obtained by RT-PCR using total RNA of activated PBMCs (cryopreserved from Zen-Bio). cDNA for full-length extracellular 4-1BB (CRD1-4 and transmembrane regions, amino acids 24–186) and each truncated form of 4-1BB (CRD1–3, 24–118; and CRD4 and transmembrane, 119–186) was ligated into the PS1121 huIgG1 Fc vector. .. For 4-1BBL.Fc, mouse 4-1BBL cDNA was obtained from total RNA of LPS-activated splenic CD11c+ DCs by RT-PCR.

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    ZenBio bp lps treatment fresh healthy human pbmcs
    Cytokine secretion by human <t>PBMCs</t> in response to treatment with B . pseudomallei <t>LPS.</t> Levels of cytokines secreted by human PBMCs were measured at 2 and 24 h post LPS treatment for Donor 1 and 24 h post treatment from Donor 2. The cytokines analyzed are indicated above each plot with the sample origins indicated on the X-axis. Data for each sample set are divided by a dashed line. Mock treated negative control replicates are indicated by black circles, data obtained for Type A (1026b) LPS treatment are indicated by blue squares, and data obtained for Type B (576a) LPS treatment are indicated by green triangles. Salmon-colored triangles indicate data from positive control S . minnesota S-LPS treated samples. A), GM-CSF; B), IFN-γ; C), IL-1β; D), IL-2; E), IL-4; F), IL-5; G), IL-6; H), IL-8; I), IL-10; J), TNF-α. Scales are either linear or log depending on the cytokine for clarity. The means of all replicates and standard error of the means are indicated by error bars and lines. Differences between 576a compared to 1026b and Salmonella compared to 1026b treated were significant as determined by multiple one-way ANOVAs as shown above the datasets. ns = not significant, * = p
    Bp Lps Treatment Fresh Healthy Human Pbmcs, supplied by ZenBio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ZenBio human pbmc gene expression analysis
    Cytokine secretion by human <t>PBMCs</t> in response to treatment with B . pseudomallei <t>LPS.</t> Levels of cytokines secreted by human PBMCs were measured at 2 and 24 h post LPS treatment for Donor 1 and 24 h post treatment from Donor 2. The cytokines analyzed are indicated above each plot with the sample origins indicated on the X-axis. Data for each sample set are divided by a dashed line. Mock treated negative control replicates are indicated by black circles, data obtained for Type A (1026b) LPS treatment are indicated by blue squares, and data obtained for Type B (576a) LPS treatment are indicated by green triangles. Salmon-colored triangles indicate data from positive control S . minnesota S-LPS treated samples. A), GM-CSF; B), IFN-γ; C), IL-1β; D), IL-2; E), IL-4; F), IL-5; G), IL-6; H), IL-8; I), IL-10; J), TNF-α. Scales are either linear or log depending on the cytokine for clarity. The means of all replicates and standard error of the means are indicated by error bars and lines. Differences between 576a compared to 1026b and Salmonella compared to 1026b treated were significant as determined by multiple one-way ANOVAs as shown above the datasets. ns = not significant, * = p
    Human Pbmc Gene Expression Analysis, supplied by ZenBio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human pbmc gene expression analysis/product/ZenBio
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human pbmc gene expression analysis - by Bioz Stars, 2020-09
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    pbmc  (ZenBio)
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    ZenBio pbmc
    EphA3 co-stains with macrophage/leukocyte markers A. Immunofluorescent staining of CD68, GFAP and Von Willebrand factor in a GBM specimen. B. Immunofluorescent staining of EphA3 (red) and CD31, GFAP, CD68, CD163, and CD206 on consecutive frozen sections of BTCOE4443 human GBM specimen. Nuclei are stained with DAPI (blue). Selected areas were magnified (last column on the right). C. Confocal immunofluorescent staining of EphA3 and CD68 in a GBM specimen using stacked 2D images. D, E. -stacked 2D images, and F. Confocal microscopy of co-staining of EphA3 and CD45 in GBM specimens. G. <t>CD4+</t> T cells do not express the EphA3 receptor. Western blot analysis of peripheral blood mononuclear cells <t>(PBMC),</t> CD4+ and CD4+ activated T cells were probed for EphA3 immunoreactivity.
    Pbmc, supplied by ZenBio, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbmc/product/ZenBio
    Average 90 stars, based on 2 article reviews
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    pbmc - by Bioz Stars, 2020-09
    90/100 stars
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    Cytokine secretion by human PBMCs in response to treatment with B . pseudomallei LPS. Levels of cytokines secreted by human PBMCs were measured at 2 and 24 h post LPS treatment for Donor 1 and 24 h post treatment from Donor 2. The cytokines analyzed are indicated above each plot with the sample origins indicated on the X-axis. Data for each sample set are divided by a dashed line. Mock treated negative control replicates are indicated by black circles, data obtained for Type A (1026b) LPS treatment are indicated by blue squares, and data obtained for Type B (576a) LPS treatment are indicated by green triangles. Salmon-colored triangles indicate data from positive control S . minnesota S-LPS treated samples. A), GM-CSF; B), IFN-γ; C), IL-1β; D), IL-2; E), IL-4; F), IL-5; G), IL-6; H), IL-8; I), IL-10; J), TNF-α. Scales are either linear or log depending on the cytokine for clarity. The means of all replicates and standard error of the means are indicated by error bars and lines. Differences between 576a compared to 1026b and Salmonella compared to 1026b treated were significant as determined by multiple one-way ANOVAs as shown above the datasets. ns = not significant, * = p

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Structural diversity of Burkholderia pseudomallei lipopolysaccharides affects innate immune signaling

    doi: 10.1371/journal.pntd.0005571

    Figure Lengend Snippet: Cytokine secretion by human PBMCs in response to treatment with B . pseudomallei LPS. Levels of cytokines secreted by human PBMCs were measured at 2 and 24 h post LPS treatment for Donor 1 and 24 h post treatment from Donor 2. The cytokines analyzed are indicated above each plot with the sample origins indicated on the X-axis. Data for each sample set are divided by a dashed line. Mock treated negative control replicates are indicated by black circles, data obtained for Type A (1026b) LPS treatment are indicated by blue squares, and data obtained for Type B (576a) LPS treatment are indicated by green triangles. Salmon-colored triangles indicate data from positive control S . minnesota S-LPS treated samples. A), GM-CSF; B), IFN-γ; C), IL-1β; D), IL-2; E), IL-4; F), IL-5; G), IL-6; H), IL-8; I), IL-10; J), TNF-α. Scales are either linear or log depending on the cytokine for clarity. The means of all replicates and standard error of the means are indicated by error bars and lines. Differences between 576a compared to 1026b and Salmonella compared to 1026b treated were significant as determined by multiple one-way ANOVAs as shown above the datasets. ns = not significant, * = p

    Article Snippet: Human PBMC gene expression analysis in response to Bp LPS treatment Fresh healthy human PBMCs were purchased from ZenBio (North Carolina, USA).

    Techniques: Negative Control, Positive Control

    Dissimilar induction of innate and adaptive immunity by Type A and B LPS in human PBMCs. RT 2 qPCR arrays were used to look at differences between Type A and B LPS in their ability to induce innate and adaptive immunity in healthy human derived PBMCs. Heat maps of expression levels of innate and adaptive immune targets from human PBMCs of two donors after 24 h treatment in mock treated (-), Type A LPS (from 1026b) treated (A), and Type B LPS (from 576a) treated (B) PBMCs. Red is the highest fold change in expression and green is the least. Log 2 gene expression scatterplots are shown below the heat-maps.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Structural diversity of Burkholderia pseudomallei lipopolysaccharides affects innate immune signaling

    doi: 10.1371/journal.pntd.0005571

    Figure Lengend Snippet: Dissimilar induction of innate and adaptive immunity by Type A and B LPS in human PBMCs. RT 2 qPCR arrays were used to look at differences between Type A and B LPS in their ability to induce innate and adaptive immunity in healthy human derived PBMCs. Heat maps of expression levels of innate and adaptive immune targets from human PBMCs of two donors after 24 h treatment in mock treated (-), Type A LPS (from 1026b) treated (A), and Type B LPS (from 576a) treated (B) PBMCs. Red is the highest fold change in expression and green is the least. Log 2 gene expression scatterplots are shown below the heat-maps.

    Article Snippet: Human PBMC gene expression analysis in response to Bp LPS treatment Fresh healthy human PBMCs were purchased from ZenBio (North Carolina, USA).

    Techniques: Real-time Polymerase Chain Reaction, Derivative Assay, Expressing

    Cytokine secretion by human PBMCs in response to treatment with B . pseudomallei LPS. Levels of cytokines secreted by human PBMCs were measured at 2 and 24 h post LPS treatment for Donor 1 and 24 h post treatment from Donor 2. The cytokines analyzed are indicated above each plot with the sample origins indicated on the X-axis. Data for each sample set are divided by a dashed line. Mock treated negative control replicates are indicated by black circles, data obtained for Type A (1026b) LPS treatment are indicated by blue squares, and data obtained for Type B (576a) LPS treatment are indicated by green triangles. Salmon-colored triangles indicate data from positive control S . minnesota S-LPS treated samples. A), GM-CSF; B), IFN-γ; C), IL-1β; D), IL-2; E), IL-4; F), IL-5; G), IL-6; H), IL-8; I), IL-10; J), TNF-α. Scales are either linear or log depending on the cytokine for clarity. The means of all replicates and standard error of the means are indicated by error bars and lines. Differences between 576a compared to 1026b and Salmonella compared to 1026b treated were significant as determined by multiple one-way ANOVAs as shown above the datasets. ns = not significant, * = p

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Structural diversity of Burkholderia pseudomallei lipopolysaccharides affects innate immune signaling

    doi: 10.1371/journal.pntd.0005571

    Figure Lengend Snippet: Cytokine secretion by human PBMCs in response to treatment with B . pseudomallei LPS. Levels of cytokines secreted by human PBMCs were measured at 2 and 24 h post LPS treatment for Donor 1 and 24 h post treatment from Donor 2. The cytokines analyzed are indicated above each plot with the sample origins indicated on the X-axis. Data for each sample set are divided by a dashed line. Mock treated negative control replicates are indicated by black circles, data obtained for Type A (1026b) LPS treatment are indicated by blue squares, and data obtained for Type B (576a) LPS treatment are indicated by green triangles. Salmon-colored triangles indicate data from positive control S . minnesota S-LPS treated samples. A), GM-CSF; B), IFN-γ; C), IL-1β; D), IL-2; E), IL-4; F), IL-5; G), IL-6; H), IL-8; I), IL-10; J), TNF-α. Scales are either linear or log depending on the cytokine for clarity. The means of all replicates and standard error of the means are indicated by error bars and lines. Differences between 576a compared to 1026b and Salmonella compared to 1026b treated were significant as determined by multiple one-way ANOVAs as shown above the datasets. ns = not significant, * = p

    Article Snippet: Human PBMC gene expression analysis in response to Bp LPS treatment Fresh healthy human PBMCs were purchased from ZenBio (North Carolina, USA).

    Techniques: Negative Control, Positive Control

    Dissimilar induction of innate and adaptive immunity by Type A and B LPS in human PBMCs. RT 2 qPCR arrays were used to look at differences between Type A and B LPS in their ability to induce innate and adaptive immunity in healthy human derived PBMCs. Heat maps of expression levels of innate and adaptive immune targets from human PBMCs of two donors after 24 h treatment in mock treated (-), Type A LPS (from 1026b) treated (A), and Type B LPS (from 576a) treated (B) PBMCs. Red is the highest fold change in expression and green is the least. Log 2 gene expression scatterplots are shown below the heat-maps.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Structural diversity of Burkholderia pseudomallei lipopolysaccharides affects innate immune signaling

    doi: 10.1371/journal.pntd.0005571

    Figure Lengend Snippet: Dissimilar induction of innate and adaptive immunity by Type A and B LPS in human PBMCs. RT 2 qPCR arrays were used to look at differences between Type A and B LPS in their ability to induce innate and adaptive immunity in healthy human derived PBMCs. Heat maps of expression levels of innate and adaptive immune targets from human PBMCs of two donors after 24 h treatment in mock treated (-), Type A LPS (from 1026b) treated (A), and Type B LPS (from 576a) treated (B) PBMCs. Red is the highest fold change in expression and green is the least. Log 2 gene expression scatterplots are shown below the heat-maps.

    Article Snippet: Human PBMC gene expression analysis in response to Bp LPS treatment Fresh healthy human PBMCs were purchased from ZenBio (North Carolina, USA).

    Techniques: Real-time Polymerase Chain Reaction, Derivative Assay, Expressing

    EphA3 co-stains with macrophage/leukocyte markers A. Immunofluorescent staining of CD68, GFAP and Von Willebrand factor in a GBM specimen. B. Immunofluorescent staining of EphA3 (red) and CD31, GFAP, CD68, CD163, and CD206 on consecutive frozen sections of BTCOE4443 human GBM specimen. Nuclei are stained with DAPI (blue). Selected areas were magnified (last column on the right). C. Confocal immunofluorescent staining of EphA3 and CD68 in a GBM specimen using stacked 2D images. D, E. -stacked 2D images, and F. Confocal microscopy of co-staining of EphA3 and CD45 in GBM specimens. G. CD4+ T cells do not express the EphA3 receptor. Western blot analysis of peripheral blood mononuclear cells (PBMC), CD4+ and CD4+ activated T cells were probed for EphA3 immunoreactivity.

    Journal: Oncotarget

    Article Title: Simultaneous targeting of Eph receptors in glioblastoma

    doi: 10.18632/oncotarget.10978

    Figure Lengend Snippet: EphA3 co-stains with macrophage/leukocyte markers A. Immunofluorescent staining of CD68, GFAP and Von Willebrand factor in a GBM specimen. B. Immunofluorescent staining of EphA3 (red) and CD31, GFAP, CD68, CD163, and CD206 on consecutive frozen sections of BTCOE4443 human GBM specimen. Nuclei are stained with DAPI (blue). Selected areas were magnified (last column on the right). C. Confocal immunofluorescent staining of EphA3 and CD68 in a GBM specimen using stacked 2D images. D, E. -stacked 2D images, and F. Confocal microscopy of co-staining of EphA3 and CD45 in GBM specimens. G. CD4+ T cells do not express the EphA3 receptor. Western blot analysis of peripheral blood mononuclear cells (PBMC), CD4+ and CD4+ activated T cells were probed for EphA3 immunoreactivity.

    Article Snippet: Isolation and activation of CD4+ T cells Total CD4+ T Cells were isolated from PBMC (ZenBio, Durham, NC) using the Dynabeads Untouched human CD4+ T-Cell Kit (Invitrogen) according to the manufacturer's instructions.

    Techniques: Staining, Confocal Microscopy, Western Blot

    Levels of cytokine formation induced by the different biotinylated α -(1 → 3)-glucan oligosaccharides. PBMCs of four donors were coincubated with biotinylated α -(1 → 3)-glucan oligosaccharides immobilized on streptavidin microtiter plates or with free natural high-molecular-weight water-insoluble α -(1 → 3)-glucan (10 μ g/mL) in suspension on nonstreptavidin plates. The coincubation was performed in RPMI supplemented with 10% normal human serum at 37 °C for 24 h. Thereafter, the supernatant was collected for quantification of the cytokines (A) TNF- α , (B) IL-6, (C) IL-1 β and (D) IL-1Ra by ELISA. The dots represent the mean cytokine concentration of triplicate evaluations of samples from each donor, and the lines represent the median of the four mean values. The cytokine concentrations induced by the oligosaccharides and the native α -(1 → 3)-glucan were compared to that of the control by nonparametric one-way ANOVA (Kruskal−Wallis test) with Dunn’s multiple comparison (**** p

    Journal: The Journal of organic chemistry

    Article Title: Chemical Synthesis and Application of Biotinylated Oligo-α-(1 → 3)-d-Glucosides To Study the Antibody and Cytokine Response against the Cell Wall α-(1 → 3)-d-Glucan of Aspergillus fumigatus

    doi: 10.1021/acs.joc.8b01142

    Figure Lengend Snippet: Levels of cytokine formation induced by the different biotinylated α -(1 → 3)-glucan oligosaccharides. PBMCs of four donors were coincubated with biotinylated α -(1 → 3)-glucan oligosaccharides immobilized on streptavidin microtiter plates or with free natural high-molecular-weight water-insoluble α -(1 → 3)-glucan (10 μ g/mL) in suspension on nonstreptavidin plates. The coincubation was performed in RPMI supplemented with 10% normal human serum at 37 °C for 24 h. Thereafter, the supernatant was collected for quantification of the cytokines (A) TNF- α , (B) IL-6, (C) IL-1 β and (D) IL-1Ra by ELISA. The dots represent the mean cytokine concentration of triplicate evaluations of samples from each donor, and the lines represent the median of the four mean values. The cytokine concentrations induced by the oligosaccharides and the native α -(1 → 3)-glucan were compared to that of the control by nonparametric one-way ANOVA (Kruskal−Wallis test) with Dunn’s multiple comparison (**** p

    Article Snippet: Finally, the PBMCs were resuspended in 20 mL of RPMI-1690 medium supplemented with 10% normal human serum (ZenBio Inc., USA).

    Techniques: Molecular Weight, Enzyme-linked Immunosorbent Assay, Concentration Assay