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u6 expression vectors  (Addgene inc)


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    Structured Review

    Addgene inc u6 expression vectors
    U6 Expression Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbluescript+vector/SpCas9_sgRNA_expression_in_pBluescript+(Plasmid+%23122089)/pmc12957561-33-23-26
    Average 93 stars, based on 12 article reviews
    u6 expression vectors - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Methylmercury Exposure during Early Xenopus laevis Development Affects Cell Proliferation and Death but not Neural Progenitor Specification
    Article Snippet: Delta was obtained in a pCMV-Sport6 vector from the IMAGE consortium (ID: 6636225; GenBank: {"type":"entrez-nucleotide","attrs":{"text":"BC070634","term_id":"47682942","term_text":"BC070634"}} BC070634 ), and antisense probe was produced by linearizing with EcoR1 and transcribing with T7 RNA polymerase. .. Engrailed-2 (En2) was obtained in the pBluescript vector from Addgene (plasmid 16950; Hemmati-Brivanlou et al. 1991 ; GenBbank: {"type":"entrez-nucleotide","attrs":{"text":"NM_001101791","term_id":"156119528","term_text":"NM_001101791"}} NM_001101791 ), and antisense probe was made by linearizing with XbaI and transcribing with T3 RNA polymerase. ..

    Article Title: The Wnt Adaptor Protein ATP6AP2 Regulates Multiple Stages of Adult Hippocampal Neurogenesis
    Article Snippet: .. The Celsr1 , 2 , and 3 cDNAs were subcloned into the pBluescript vector (Addgene) using previously described primers ( Tissir et al., 2002 ). .. RNA sense and antisense probes were synthesized using T3 or T7 RNA polymerase (Promega).

    Article Title: The Wnt Adaptor Protein ATP6AP2 Regulates Multiple Stages of Adult Hippocampal Neurogenesis
    Article Snippet: .. The Celsr1, 2, and 3 cDNAs were subcloned into the pBluescript vector (Addgene) using previously described primers (Tissir et al., 2002). .. RNA sense and antisense probes were synthesized using T3 or T7 RNA polymerase (Promega).

    Article Title: Decreased tumorigenesis in mice with a Kras point mutation at C118
    Article Snippet: .. Resultant products were cloned into the HindIII and SacI sites of pBluescript vector (from Addgene), after which 12 positive clones from each ligation reaction were sequenced using T7 promoter (Eton Bioscience) to determine whether the clone corresponded to the native or C118S allele of Kras , and whether the clone had an oncogenic mutation (at position G12, G13 and Q61). ..

    Expressing:

    Article Title: HUWE1 Amplifies Ubiquitin Modifications to Broadly Stimulate Clearance of Proteins and Aggregates
    Article Snippet: .. Plasmids expressing RFP-HUWE1/HUWE1 CA /HUWE1 UBDmu were constructed in a pBluescript vector (addgene: 72835) containing a tetracycline-inducible promoter (TetOn). ..

    Construct:

    Article Title: HUWE1 Amplifies Ubiquitin Modifications to Broadly Stimulate Clearance of Proteins and Aggregates
    Article Snippet: .. Plasmids expressing RFP-HUWE1/HUWE1 CA /HUWE1 UBDmu were constructed in a pBluescript vector (addgene: 72835) containing a tetracycline-inducible promoter (TetOn). ..

    Amplification:

    Article Title: JOINTLESS Maintains Inflorescence Meristem Identity in Tomato
    Article Snippet: Hybridization was subsequently performed as described in and manufacturer’s protocol (‘DIG Application Manual for Nonradioactive In Situ Hybridization’, 4 th Edition, Roche). .. For probe synthesis, SP (Solyc06g074350), BRC1a (Solyc03g119770) and BRC1b (Solyc06g069240) coding sequences were amplified by PCR (primers in ) using cDNA from young inflorescences (AC accession) as the template and the resulting products were cloned into pBlueScript vector (Addgene, Watertown, Massachusetts, USA). ..

    Polymerase Chain Reaction:

    Article Title: JOINTLESS Maintains Inflorescence Meristem Identity in Tomato
    Article Snippet: Hybridization was subsequently performed as described in and manufacturer’s protocol (‘DIG Application Manual for Nonradioactive In Situ Hybridization’, 4 th Edition, Roche). .. For probe synthesis, SP (Solyc06g074350), BRC1a (Solyc03g119770) and BRC1b (Solyc06g069240) coding sequences were amplified by PCR (primers in ) using cDNA from young inflorescences (AC accession) as the template and the resulting products were cloned into pBlueScript vector (Addgene, Watertown, Massachusetts, USA). ..

    Clone Assay:

    Article Title: JOINTLESS Maintains Inflorescence Meristem Identity in Tomato
    Article Snippet: Hybridization was subsequently performed as described in and manufacturer’s protocol (‘DIG Application Manual for Nonradioactive In Situ Hybridization’, 4 th Edition, Roche). .. For probe synthesis, SP (Solyc06g074350), BRC1a (Solyc03g119770) and BRC1b (Solyc06g069240) coding sequences were amplified by PCR (primers in ) using cDNA from young inflorescences (AC accession) as the template and the resulting products were cloned into pBlueScript vector (Addgene, Watertown, Massachusetts, USA). ..

    Article Title: Decreased tumorigenesis in mice with a Kras point mutation at C118
    Article Snippet: .. Resultant products were cloned into the HindIII and SacI sites of pBluescript vector (from Addgene), after which 12 positive clones from each ligation reaction were sequenced using T7 promoter (Eton Bioscience) to determine whether the clone corresponded to the native or C118S allele of Kras , and whether the clone had an oncogenic mutation (at position G12, G13 and Q61). ..

    Ligation:

    Article Title: Decreased tumorigenesis in mice with a Kras point mutation at C118
    Article Snippet: .. Resultant products were cloned into the HindIII and SacI sites of pBluescript vector (from Addgene), after which 12 positive clones from each ligation reaction were sequenced using T7 promoter (Eton Bioscience) to determine whether the clone corresponded to the native or C118S allele of Kras , and whether the clone had an oncogenic mutation (at position G12, G13 and Q61). ..

    Mutagenesis:

    Article Title: Decreased tumorigenesis in mice with a Kras point mutation at C118
    Article Snippet: .. Resultant products were cloned into the HindIII and SacI sites of pBluescript vector (from Addgene), after which 12 positive clones from each ligation reaction were sequenced using T7 promoter (Eton Bioscience) to determine whether the clone corresponded to the native or C118S allele of Kras , and whether the clone had an oncogenic mutation (at position G12, G13 and Q61). ..



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    Image Search Results


    Journal: bioRxiv

    Article Title: CANVAS causing AAGGG repeat expansions cause tissue-specific reduction in RFC1 expression and increase sensitivity to DNA damage

    doi: 10.1101/2025.11.18.688292

    Figure Lengend Snippet:

    Article Snippet: Briefly, Firefly Luciferase plasmid and the repeat containing pBluescript vectors were digested with XbaI and NotI restriction enzymes (NEB), while pGint plasmid and pBluescript vectors were digested with NotI and BamHI restriction enzymes (NEB) at 37°C for 1 hour.

    Techniques: Luciferase