dsm 25962 t  (DSMZ)

Journal: Antonie Van Leeuwenhoek

Article Title: New genus-specific primers for PCR identification of Rubrobacter strains

doi: 10.1007/s10482-019-01314-3

Figure Lengend Snippet: Type strains of Rubrobacter species and those of related genera, their growth requirements and 16S rRNA gene sequence accession numbers

Article Snippet: Patulibacter medicamentivorans , DSM 25962 T , DSMZ medium No. 830 , 28 , AGUD01000068 , Almeida et al. ( ) .

Techniques: Sequencing

Journal: Antonie Van Leeuwenhoek

Article Title: New genus-specific primers for PCR identification of Rubrobacter strains

doi: 10.1007/s10482-019-01314-3

Figure Lengend Snippet: Conserved nucleotide regions of 16S rRNA genes of Rubrobacter type strains used to design the specific primers Rubro223f and Rubro454r. The bar represents the 16S rRNA gene sequence of Escherichia coli ; black boxes indicate conserved regions and the grey ones variable regions (V) with corresponding numbers (Brosius et al. ; Yarza et al. ). Arrows above the bar represent the position of the primers within the 16S rRNA gene sequence. Nucleotide alignment for primers Rubro223f and Rubro454r and for primer Rubro749r (Holmes et al. ) are highlighted in orange boxes and nucleotides in white represent those unique to the genus Rubrobacter and hence absent in the type strains of species classified in the genera Conexibacter , Gaiella , Parviterribacter , Patulibacter, Solirubrobacter and Thermoleophilum . (Color figure online)

Article Snippet: Patulibacter medicamentivorans , DSM 25962 T , DSMZ medium No. 830 , 28 , AGUD01000068 , Almeida et al. ( ) .

Techniques: Sequencing

Journal: Antonie Van Leeuwenhoek

Article Title: New genus-specific primers for PCR identification of Rubrobacter strains

doi: 10.1007/s10482-019-01314-3

Figure Lengend Snippet: Maximum-likelihood phylogenetic tree generated using the GTR + CAT model and rooted by midpoint-rooting showing relationships between the 267 nt sequences amplified with primers Rubro223f and Rubro454r, using community DNA extracted from Salar de Tara (ST1) and Quebrada Nacimiento (QN) soils and the corresponding full 16S rRNA gene sequences of the type strains of representatives of the genera Conexibacter , Gaiella , Parviterribacter , Patulibacter, Solirubrobacter and Thermoleophilum . The branches of the tree are scaled in terms of the expected number of substitutions per site and the numbers above the branches are bootstrap support values greater than 60% for the ML (left) and MP (right) analyses

Article Snippet: Patulibacter medicamentivorans , DSM 25962 T , DSMZ medium No. 830 , 28 , AGUD01000068 , Almeida et al. ( ) .

Techniques: Generated, Amplification