pasteur pipettes novagen pellet paint coprecipitant phenol proteinase k transcription stop buffer  (Millipore)


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    Millipore pasteur pipettes novagen pellet paint coprecipitant phenol proteinase k transcription stop buffer
    Pasteur Pipettes Novagen Pellet Paint Coprecipitant Phenol Proteinase K Transcription Stop Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pasteur pipettes novagen pellet paint coprecipitant phenol proteinase k transcription stop buffer/product/Millipore
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pasteur pipettes novagen pellet paint coprecipitant phenol proteinase k transcription stop buffer - by Bioz Stars, 2020-03
    79/100 stars

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    Concentration Assay:

    Article Title: In vitro transcription and immobilized template analysis of preinitiation complexes
    Article Snippet: .. Acetyl-Coenzyme A (1 mM) Activator protein (200 ng/µL in 0.1 M Buffer D) Buffer D (0.1 M KCl; See Recipes) Chloroform DNA template (in this protocol, the authors use a 602-bp linear chromatin template containing the G5E4T promoter at a starting concentration of 5 ng/µL) Dry Ice Ethanol (100%) Formamide Loading Dye (See Recipes) MgCl2 (0.1 M) Nonspecific Competitor DNA (in this protocol the authors use the plasmid vector used in cloning the G5E4T template promoter: pGEM3 at a starting concentration of 100 µg/mL) NTPs (25 mM each, ATP, UTP, CTP and GTP) HeLa Nuclear Extract (Support Protocol 3) Pasteur Pipettes Novagen™ Pellet Paint Coprecipitant Phenol Proteinase K Transcription Stop Buffer (See Recipes) .. NOTE: If immobilized templates are to be used, beads containing the immobilized promoter should be resuspended in the appropriate amount of 0.1 M Buffer D and incubated with the desired proteins (e.g., activator, co-activator complexes) as described in Basic Protocol 2 (Immobilized DNA template assay) before resuspending in up to 10 µL of 0.1 M Buffer D. Skip to Step 3.

    Plasmid Preparation:

    Article Title: In vitro transcription and immobilized template analysis of preinitiation complexes
    Article Snippet: .. Acetyl-Coenzyme A (1 mM) Activator protein (200 ng/µL in 0.1 M Buffer D) Buffer D (0.1 M KCl; See Recipes) Chloroform DNA template (in this protocol, the authors use a 602-bp linear chromatin template containing the G5E4T promoter at a starting concentration of 5 ng/µL) Dry Ice Ethanol (100%) Formamide Loading Dye (See Recipes) MgCl2 (0.1 M) Nonspecific Competitor DNA (in this protocol the authors use the plasmid vector used in cloning the G5E4T template promoter: pGEM3 at a starting concentration of 100 µg/mL) NTPs (25 mM each, ATP, UTP, CTP and GTP) HeLa Nuclear Extract (Support Protocol 3) Pasteur Pipettes Novagen™ Pellet Paint Coprecipitant Phenol Proteinase K Transcription Stop Buffer (See Recipes) .. NOTE: If immobilized templates are to be used, beads containing the immobilized promoter should be resuspended in the appropriate amount of 0.1 M Buffer D and incubated with the desired proteins (e.g., activator, co-activator complexes) as described in Basic Protocol 2 (Immobilized DNA template assay) before resuspending in up to 10 µL of 0.1 M Buffer D. Skip to Step 3.

    Clone Assay:

    Article Title: In vitro transcription and immobilized template analysis of preinitiation complexes
    Article Snippet: .. Acetyl-Coenzyme A (1 mM) Activator protein (200 ng/µL in 0.1 M Buffer D) Buffer D (0.1 M KCl; See Recipes) Chloroform DNA template (in this protocol, the authors use a 602-bp linear chromatin template containing the G5E4T promoter at a starting concentration of 5 ng/µL) Dry Ice Ethanol (100%) Formamide Loading Dye (See Recipes) MgCl2 (0.1 M) Nonspecific Competitor DNA (in this protocol the authors use the plasmid vector used in cloning the G5E4T template promoter: pGEM3 at a starting concentration of 100 µg/mL) NTPs (25 mM each, ATP, UTP, CTP and GTP) HeLa Nuclear Extract (Support Protocol 3) Pasteur Pipettes Novagen™ Pellet Paint Coprecipitant Phenol Proteinase K Transcription Stop Buffer (See Recipes) .. NOTE: If immobilized templates are to be used, beads containing the immobilized promoter should be resuspended in the appropriate amount of 0.1 M Buffer D and incubated with the desired proteins (e.g., activator, co-activator complexes) as described in Basic Protocol 2 (Immobilized DNA template assay) before resuspending in up to 10 µL of 0.1 M Buffer D. Skip to Step 3.