paper emdb emd 28253 cryo em map  (Thermo Fisher)

Journal: Cell

Article Title: Structures of LRP2 reveal a molecular machine for endocytosis

doi: 10.1016/j.cell.2023.01.016

Figure Lengend Snippet: (A) Cryo-EM map of LRP2 at pH 7.5. One protomer is colored in rainbow, from the N-terminus (purple) to the C-terminus (red); the other is in wheat. Distinctive regions are labelled, and dimensions are shown. Coloring and labeling is consistent throughout the figure. Distance between the transmembrane insertions is labeled.

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins PMSF Alexis Cat# 270–184-G005 BSA Gemini Cat# 700–100P Mannitol Millipore Sigma Cat# M4125 Tris 1M, pH 7.5 Corning Cat# 46–030-CM Sodium Chloride Fischer Scientific Cat# S671 Sucrose Fischer Cat# S25590B Glycerol Millipore Sigma Cat# G5516 Pepstatin A Millipore Sigma Cat# P5318 Leupeptin Millipore Sigma Cat# L2884 β-octylglucoside RPI Cat# N02007 Protease inhibitor cocktail Millipore Sigma Cat# P8340 Anti-LRP2 antibody Abcam Cat# ab76969 Aprotinin Millipore Sigma Cat# 10236624001 Sodium acetate Millipore Sigma Cat #S7545 Tween-20 Millipore Sigma Cat #P7949 HEPES Millipore Sigma Cat #H3375 Calcium chloride Millipore Sigma Cat# C3881 Amine coupling kit Cytiva Cat# BR100050 Critical commercial assays SuperLoop Cytiva Cat# 18102385 HiTrap Q HP Cytiva Cat# 17115301 Amicron ultra centrifugal filter units Millipore Sigma Cat# UFC210024 Series S Sensor Chip CM4 Cytiva Cat #BR100534 Superose 6 30/300 Cytiva Cat# 17517201 Recombinant DNA pGEX-KG-RAP Joachim Herz Lab N/A Deposited data Cryo-EM structure of LRP2 at pH 7.5 This paper PDB: 8EM4 Cryo-EM map of LRP2 at pH 7.5 This paper EMDB: EMD-28233 Cryo-EM map of local refinement of P1-P2 domains of LRP2 at pH 7.5 This paper EMDB: EMD-28242 Cryo-EM map of local refinement of P3-P6 domains of LRP2 at pH 7.5 This paper EMDB: EMD-28243 Cryo-EM map of local refinement of P7 domain of LRP2 at pH 7.5 This paper EMDB: EMD-28250 Cryo-EM map of local refinement of R4 domain of LRP2 at pH 7.5 This paper EMDB: EMD-28251 Cryo-EM map of local refinement of P8 domain of LRP2 at pH 7.5 This paper EMDB: EMD-28252 Cryo-EM structure of LRP2 at pH 5.2 This paper PDB: 8EM7 Cryo-EM map of LRP2 at pH 5.2 This paper EMDB: EMD-28241 Cryo-EM map of local refinement of P1-P2 domains of LRP2 at pH 5.2 This paper EMDB: EMD-28253 Cryo-EM map of local refinement of P1-P2 domains of LRP2 at pH 5.2 This paper EMDB: EMD-28258 Cryo-EM map of local refinement of P1-P2 domains of LRP2 at pH 5.2 This paper EMDB: EMD-28260 Cryo-EM map of local refinement of P1-P2 domains of LRP2 at pH 5.2 This paper EMDB: EMD-28261 Cryo-EM map of local refinement of P1-P2 domains of LRP2 at pH 5.2 This paper EMBD: EMD-28265 Software and algorithms Coot Emsley et al. 143 , 160 https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot cryoSPARC Punjani et al. 118 https://cryosparc.com Leginon Suloway et al. 137 https://sbgrid.org/software/titles/leginon Molprobity Davis et al. 152 http://molprobity.biochem.duke.edu Phenix Adams et al. 161 https://www.phenix-online.org The PyMOL Molecular Graphics System, Version 2.0 Schrödinger, LLC https://pymol.org/2/support.html#page-top RELION Scheres, S.H.

Techniques: Cryo-EM Sample Prep, Labeling