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Illumina Inc paired end sequencing library kit
Paired End Sequencing Library Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/paired end sequencing library kit/product/Illumina Inc
Average 92 stars, based on 7 article reviews
Price from $9.99 to $1999.99
paired end sequencing library kit - by Bioz Stars, 2019-10
92/100 stars

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Related Articles

Amplification:

Article Title: Pilot study of sirolimus in patients with PIK3CA mutant/amplified refractory solid cancer
Article Snippet: PIK3CA amplification/mutation was detected by targeted deep sequencing by CancerSCAN™ ( ). .. Briefly, extracted genomic DNA was sheared to 150–200 bp fragments using Covaris S220 (Covaris, Woburn, MA, USA) and targeted genes were captured using a custom panel capture library (Agilent Technologies, Inc., Santa Clara, CA, USA) for 2.5 Mb of exonic regions with an Illumina Paired-End Sequencing Library kit.

Microscopy:

Article Title: MAP2K1 Mutation in Colorectal Cancer Patients: Therapeutic Challenge Using Patient-Derived Tumor Cell Lines
Article Snippet: Formalin-fixed paraffin embedded (FFPE) samples containing > 40% tumor cellularity were dissected under a microscope from 4-μm thick unstained sections (10 to 20 slides) or from fresh frozen tissues by comparison with a hematoxylin and eosin-stained slide. .. Targeted genes were captured using a custom panel capture library (Agilent Technologies, Santa Clara, CA, USA) covering 2.5 Mb of exonic regions for the Illumina Paired-End Sequencing Library kit.

Article Title: Antitumor activity of sorafenib plus CDK4/6 inhibitor in pancreatic patient derived cell with KRAS mutation
Article Snippet: Formalin-fixed paraffin embedded (FFPE) samples containing > 40% tumor cellularity were dissected under microscopy from 4-μm-thick unstained sections (10 to 20 slides) or from fresh frozen tissues by comparison with a hematoxylin and eosin-stained slide. .. Briefly, DNA was extracted using standard procedures (Qiagen, Hilden, Germany) and extracted genomic DNA was sheared to 150-200 bp using Covaris S220 (Covaris, Woburn, MA, USA), and targeted genes were captured using a custom panel capture library (Agilent Technologies, Santa Clara, CA, USA) covering 2.5 Mb of exonic regions for the Illumina Paired-End Sequencing Library kit.

Digital PCR:

Article Title: Antitumor activity of sorafenib plus CDK4/6 inhibitor in pancreatic patient derived cell with KRAS mutation
Article Snippet: Briefly, DNA was extracted using standard procedures (Qiagen, Hilden, Germany) and extracted genomic DNA was sheared to 150-200 bp using Covaris S220 (Covaris, Woburn, MA, USA), and targeted genes were captured using a custom panel capture library (Agilent Technologies, Santa Clara, CA, USA) covering 2.5 Mb of exonic regions for the Illumina Paired-End Sequencing Library kit. .. We aligned the sequencing reads to the human reference genome (GRCh37/hg19), excluded duplicated reads, and extracted uniquely mapped and properly paired reads with an insert size.

Sequencing:

Article Title: Deep sequencing reveals clonal evolution patterns and mutation events associated with relapse in B-cell lymphomas
Article Snippet: For each MiSeq sequencing run, five VDJ samples were mixed together at 7 μM with 50% PhiX spike-in to ensure the complexity of the run. .. Exome sequencing samples were prepared using the Aglient SureSelectXT Human All Exon 50 MB Target Enrichment System for Illumina Paired-End Sequencing Library kit. .. PE75 sequencing was performed on Illumina HiSeq 2000.

Article Title: MAP2K1 Mutation in Colorectal Cancer Patients: Therapeutic Challenge Using Patient-Derived Tumor Cell Lines
Article Snippet: Briefly, DNA was extracted using standard procedures (Qiagen) and extracted genomic DNA was sheared to 150-200 bp using Covaris S220 (Covaris, Woburn, MA, USA). .. Targeted genes were captured using a custom panel capture library (Agilent Technologies, Santa Clara, CA, USA) covering 2.5 Mb of exonic regions for the Illumina Paired-End Sequencing Library kit. .. We performed DNA sequencing of 100- or 101-bp paired-end reads using the Illumina HiSeq 2,500 sequencer (Illumina, San Diego, CA, USA).

Article Title: Large-scale recent expansion of European patrilineages shown by population resequencing
Article Snippet: The total length of targeted regions was ∼8.6 Mb, and following capture design and the necessary repeat masking, the designed baits covered 2.3 Mb. .. Genomic DNA (3–5 μg) was used for library preparation and target enrichment using Agilent SureSelectXT Target Enrichment System for Illumina Paired-End Sequencing Library kit (version 1.3). .. In order to obtain larger insert sizes, DNA samples were fragmented to ∼250–600 bp without size selection.

Article Title: Antitumor activity of sorafenib plus CDK4/6 inhibitor in pancreatic patient derived cell with KRAS mutation
Article Snippet: Formalin-fixed paraffin embedded (FFPE) samples containing > 40% tumor cellularity were dissected under microscopy from 4-μm-thick unstained sections (10 to 20 slides) or from fresh frozen tissues by comparison with a hematoxylin and eosin-stained slide. .. Briefly, DNA was extracted using standard procedures (Qiagen, Hilden, Germany) and extracted genomic DNA was sheared to 150-200 bp using Covaris S220 (Covaris, Woburn, MA, USA), and targeted genes were captured using a custom panel capture library (Agilent Technologies, Santa Clara, CA, USA) covering 2.5 Mb of exonic regions for the Illumina Paired-End Sequencing Library kit. .. We performed DNA sequencing of 100 or 101-bp paired-end reads using the Illumina HiSeq 2,500 sequencer (Illumina, San Diego, CA, USA).

Article Title: Pilot study of sirolimus in patients with PIK3CA mutant/amplified refractory solid cancer
Article Snippet: PIK3CA amplification/mutation was detected by targeted deep sequencing by CancerSCAN™ ( ). .. Briefly, extracted genomic DNA was sheared to 150–200 bp fragments using Covaris S220 (Covaris, Woburn, MA, USA) and targeted genes were captured using a custom panel capture library (Agilent Technologies, Inc., Santa Clara, CA, USA) for 2.5 Mb of exonic regions with an Illumina Paired-End Sequencing Library kit. .. DNA sequencing of 100 or 101-bp paired-end reads was performed using the Illumina HiSeq 2,500 sequencer (Illumina, Inc., San Diego, CA, USA).

Formalin-fixed Paraffin-Embedded:

Article Title: MAP2K1 Mutation in Colorectal Cancer Patients: Therapeutic Challenge Using Patient-Derived Tumor Cell Lines
Article Snippet: Formalin-fixed paraffin embedded (FFPE) samples containing > 40% tumor cellularity were dissected under a microscope from 4-μm thick unstained sections (10 to 20 slides) or from fresh frozen tissues by comparison with a hematoxylin and eosin-stained slide. .. Targeted genes were captured using a custom panel capture library (Agilent Technologies, Santa Clara, CA, USA) covering 2.5 Mb of exonic regions for the Illumina Paired-End Sequencing Library kit.

Article Title: Antitumor activity of sorafenib plus CDK4/6 inhibitor in pancreatic patient derived cell with KRAS mutation
Article Snippet: Formalin-fixed paraffin embedded (FFPE) samples containing > 40% tumor cellularity were dissected under microscopy from 4-μm-thick unstained sections (10 to 20 slides) or from fresh frozen tissues by comparison with a hematoxylin and eosin-stained slide. .. Briefly, DNA was extracted using standard procedures (Qiagen, Hilden, Germany) and extracted genomic DNA was sheared to 150-200 bp using Covaris S220 (Covaris, Woburn, MA, USA), and targeted genes were captured using a custom panel capture library (Agilent Technologies, Santa Clara, CA, USA) covering 2.5 Mb of exonic regions for the Illumina Paired-End Sequencing Library kit.

High Throughput Screening Assay:

Article Title: Large-scale recent expansion of European patrilineages shown by population resequencing
Article Snippet: Genomic DNA (3–5 μg) was used for library preparation and target enrichment using Agilent SureSelectXT Target Enrichment System for Illumina Paired-End Sequencing Library kit (version 1.3). .. Sequencing was done on an Illumina HiSeq 2000 instrument (Illumina, CA, USA) with paired-end 100-bp run to high coverage.

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    Illumina Inc paired end sequencing library kit
    Paired End Sequencing Library Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/paired end sequencing library kit/product/Illumina Inc
    Average 92 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    paired end sequencing library kit - by Bioz Stars, 2019-10
    92/100 stars
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