pacv5 hisa  (Thermo Fisher)


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  • 87

    Structured Review

    Thermo Fisher pacv5 hisa
    Pacv5 Hisa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pacv5 hisa/product/Thermo Fisher
    Average 87 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    pacv5 hisa - by Bioz Stars, 2020-04
    87/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Common and distinct DNA-binding and regulatory activities of the BEN-solo transcription factor family
    Article Snippet: .. Insv-BEN, Bsg25A-BEN, Elba2-BEN, BEND5-BEN, BEND6-BEN, BANP-BEN, and NAC2-BEN fragments were created by PCR amplification from Insv, Bsg25A, Elba2, human BEND5, mouse BEND6, mouse BANP, and human NAC2 cDNAs and cloned at EcoRI and XhoI sites (for Insv, BEND5, BEND6, BANP, and NAC2) or NotI and XhoI sites (for Bsg25A and Elba2) in pAcV5/HisA (Invitrogen). .. These plasmids were digested with XhoI and XbaI sites and ligated with a DNA fragment with the same sites encoding the VP16 activation domain to generate VP16 fusion constructs.

    Article Title: The BEN domain is a novel sequence-specific DNA-binding domain conserved in neural transcriptional repressors
    Article Snippet: .. Insv-N, Insv-C, and BEND5C fragments were created by PCR amplification from Insv and BEND5 cDNAs with primers containing EcoRI and XhoI sites (sequences listed in Supplemental Table 4) and cloned into the EcoRI and XhoI sites of pAcV5/HisA (Invitrogen). .. These plasmids were digested with XhoI and XbaI sites and ligated with a DNA fragment with the same sites encoding VP16 activation domain, yielding pAc-Insv-VP16, pAc-InsvN-VP16, pAc-InsvC-VP16, and pAc-BEND5C-VP16 constructs.

    Amplification:

    Article Title: Common and distinct DNA-binding and regulatory activities of the BEN-solo transcription factor family
    Article Snippet: .. Insv-BEN, Bsg25A-BEN, Elba2-BEN, BEND5-BEN, BEND6-BEN, BANP-BEN, and NAC2-BEN fragments were created by PCR amplification from Insv, Bsg25A, Elba2, human BEND5, mouse BEND6, mouse BANP, and human NAC2 cDNAs and cloned at EcoRI and XhoI sites (for Insv, BEND5, BEND6, BANP, and NAC2) or NotI and XhoI sites (for Bsg25A and Elba2) in pAcV5/HisA (Invitrogen). .. These plasmids were digested with XhoI and XbaI sites and ligated with a DNA fragment with the same sites encoding the VP16 activation domain to generate VP16 fusion constructs.

    Article Title: The BEN domain is a novel sequence-specific DNA-binding domain conserved in neural transcriptional repressors
    Article Snippet: .. Insv-N, Insv-C, and BEND5C fragments were created by PCR amplification from Insv and BEND5 cDNAs with primers containing EcoRI and XhoI sites (sequences listed in Supplemental Table 4) and cloned into the EcoRI and XhoI sites of pAcV5/HisA (Invitrogen). .. These plasmids were digested with XhoI and XbaI sites and ligated with a DNA fragment with the same sites encoding VP16 activation domain, yielding pAc-Insv-VP16, pAc-InsvN-VP16, pAc-InsvC-VP16, and pAc-BEND5C-VP16 constructs.

    Polymerase Chain Reaction:

    Article Title: Common and distinct DNA-binding and regulatory activities of the BEN-solo transcription factor family
    Article Snippet: .. Insv-BEN, Bsg25A-BEN, Elba2-BEN, BEND5-BEN, BEND6-BEN, BANP-BEN, and NAC2-BEN fragments were created by PCR amplification from Insv, Bsg25A, Elba2, human BEND5, mouse BEND6, mouse BANP, and human NAC2 cDNAs and cloned at EcoRI and XhoI sites (for Insv, BEND5, BEND6, BANP, and NAC2) or NotI and XhoI sites (for Bsg25A and Elba2) in pAcV5/HisA (Invitrogen). .. These plasmids were digested with XhoI and XbaI sites and ligated with a DNA fragment with the same sites encoding the VP16 activation domain to generate VP16 fusion constructs.

    Article Title: The BEN domain is a novel sequence-specific DNA-binding domain conserved in neural transcriptional repressors
    Article Snippet: .. Insv-N, Insv-C, and BEND5C fragments were created by PCR amplification from Insv and BEND5 cDNAs with primers containing EcoRI and XhoI sites (sequences listed in Supplemental Table 4) and cloned into the EcoRI and XhoI sites of pAcV5/HisA (Invitrogen). .. These plasmids were digested with XhoI and XbaI sites and ligated with a DNA fragment with the same sites encoding VP16 activation domain, yielding pAc-Insv-VP16, pAc-InsvN-VP16, pAc-InsvC-VP16, and pAc-BEND5C-VP16 constructs.

    Mutagenesis:

    Article Title: The BEN domain is a novel sequence-specific DNA-binding domain conserved in neural transcriptional repressors
    Article Snippet: To mutate the corresponding bases in the residues, Ser304, Asp351, and Lys354 primer oligos encoding mutant residues were designed, and site mutagenesis was performed according to Stratagene site mutagenesis protocol. .. Insv-N, Insv-C, and BEND5C fragments were created by PCR amplification from Insv and BEND5 cDNAs with primers containing EcoRI and XhoI sites (sequences listed in Supplemental Table 4) and cloned into the EcoRI and XhoI sites of pAcV5/HisA (Invitrogen).

    Construct:

    Article Title: Common and distinct DNA-binding and regulatory activities of the BEN-solo transcription factor family
    Article Snippet: Insv-BEN, Bsg25A-BEN, Elba2-BEN, BEND5-BEN, BEND6-BEN, BANP-BEN, and NAC2-BEN fragments were created by PCR amplification from Insv, Bsg25A, Elba2, human BEND5, mouse BEND6, mouse BANP, and human NAC2 cDNAs and cloned at EcoRI and XhoI sites (for Insv, BEND5, BEND6, BANP, and NAC2) or NotI and XhoI sites (for Bsg25A and Elba2) in pAcV5/HisA (Invitrogen). .. These plasmids were digested with XhoI and XbaI sites and ligated with a DNA fragment with the same sites encoding the VP16 activation domain to generate VP16 fusion constructs.

    Article Title: The BEN domain is a novel sequence-specific DNA-binding domain conserved in neural transcriptional repressors
    Article Snippet: An Insv-V5 fusion construct was generated by inserting an insv ORF sequence with a mutated stop codon to the NotI and XhoI sites of the pAcV5/HisA vector (Invitrogen). .. Insv-N, Insv-C, and BEND5C fragments were created by PCR amplification from Insv and BEND5 cDNAs with primers containing EcoRI and XhoI sites (sequences listed in Supplemental Table 4) and cloned into the EcoRI and XhoI sites of pAcV5/HisA (Invitrogen).

    Activation Assay:

    Article Title: Common and distinct DNA-binding and regulatory activities of the BEN-solo transcription factor family
    Article Snippet: Insv-BEN, Bsg25A-BEN, Elba2-BEN, BEND5-BEN, BEND6-BEN, BANP-BEN, and NAC2-BEN fragments were created by PCR amplification from Insv, Bsg25A, Elba2, human BEND5, mouse BEND6, mouse BANP, and human NAC2 cDNAs and cloned at EcoRI and XhoI sites (for Insv, BEND5, BEND6, BANP, and NAC2) or NotI and XhoI sites (for Bsg25A and Elba2) in pAcV5/HisA (Invitrogen). .. These plasmids were digested with XhoI and XbaI sites and ligated with a DNA fragment with the same sites encoding the VP16 activation domain to generate VP16 fusion constructs.

    Article Title: The BEN domain is a novel sequence-specific DNA-binding domain conserved in neural transcriptional repressors
    Article Snippet: Insv-N, Insv-C, and BEND5C fragments were created by PCR amplification from Insv and BEND5 cDNAs with primers containing EcoRI and XhoI sites (sequences listed in Supplemental Table 4) and cloned into the EcoRI and XhoI sites of pAcV5/HisA (Invitrogen). .. These plasmids were digested with XhoI and XbaI sites and ligated with a DNA fragment with the same sites encoding VP16 activation domain, yielding pAc-Insv-VP16, pAc-InsvN-VP16, pAc-InsvC-VP16, and pAc-BEND5C-VP16 constructs.

    Generated:

    Article Title: Common and distinct DNA-binding and regulatory activities of the BEN-solo transcription factor family
    Article Snippet: Insv-BEN, Bsg25A-BEN, Elba2-BEN, BEND5-BEN, BEND6-BEN, BANP-BEN, and NAC2-BEN fragments were created by PCR amplification from Insv, Bsg25A, Elba2, human BEND5, mouse BEND6, mouse BANP, and human NAC2 cDNAs and cloned at EcoRI and XhoI sites (for Insv, BEND5, BEND6, BANP, and NAC2) or NotI and XhoI sites (for Bsg25A and Elba2) in pAcV5/HisA (Invitrogen). .. We generated multicistronic 2A constructs as follows.

    Article Title: The BEN domain is a novel sequence-specific DNA-binding domain conserved in neural transcriptional repressors
    Article Snippet: An Insv-V5 fusion construct was generated by inserting an insv ORF sequence with a mutated stop codon to the NotI and XhoI sites of the pAcV5/HisA vector (Invitrogen). .. Insv-N, Insv-C, and BEND5C fragments were created by PCR amplification from Insv and BEND5 cDNAs with primers containing EcoRI and XhoI sites (sequences listed in Supplemental Table 4) and cloned into the EcoRI and XhoI sites of pAcV5/HisA (Invitrogen).

    Luciferase:

    Article Title: The BEN domain is a novel sequence-specific DNA-binding domain conserved in neural transcriptional repressors
    Article Snippet: Paragraph title: Luciferase reporter assays ... Insv-N, Insv-C, and BEND5C fragments were created by PCR amplification from Insv and BEND5 cDNAs with primers containing EcoRI and XhoI sites (sequences listed in Supplemental Table 4) and cloned into the EcoRI and XhoI sites of pAcV5/HisA (Invitrogen).

    Sequencing:

    Article Title: The BEN domain is a novel sequence-specific DNA-binding domain conserved in neural transcriptional repressors
    Article Snippet: An Insv-V5 fusion construct was generated by inserting an insv ORF sequence with a mutated stop codon to the NotI and XhoI sites of the pAcV5/HisA vector (Invitrogen). .. Insv-N, Insv-C, and BEND5C fragments were created by PCR amplification from Insv and BEND5 cDNAs with primers containing EcoRI and XhoI sites (sequences listed in Supplemental Table 4) and cloned into the EcoRI and XhoI sites of pAcV5/HisA (Invitrogen).

    Plasmid Preparation:

    Article Title: The BEN domain is a novel sequence-specific DNA-binding domain conserved in neural transcriptional repressors
    Article Snippet: An Insv-V5 fusion construct was generated by inserting an insv ORF sequence with a mutated stop codon to the NotI and XhoI sites of the pAcV5/HisA vector (Invitrogen). .. Insv-N, Insv-C, and BEND5C fragments were created by PCR amplification from Insv and BEND5 cDNAs with primers containing EcoRI and XhoI sites (sequences listed in Supplemental Table 4) and cloned into the EcoRI and XhoI sites of pAcV5/HisA (Invitrogen).