pa103  (ATCC)

Journal: Journal of Bacteriology

Article Title: Biosynthesis of glucosaminyl phosphatidylglycerol in Pseudomonas aeruginosa

doi: 10.1128/jb.00490-24

Figure Lengend Snippet: Detection of GlcN-PG in various P. aeruginosa laboratory and clinical strains by LC/MS. Shown are the negative ion mass spectra of the [M–H] − ions of GlcN-PG species in ( A ) PA103, ( B ) ATCC 27853, and ( C ) PAO1 . The identification of GlcN-PG molecular species is confirmed by exact mass measurement and MS/MS.

Article Snippet: PA103 (ATCC 29260) , Wild type , ( ) .

Techniques: Liquid Chromatography with Mass Spectroscopy, Mass Measurement, Tandem Mass Spectroscopy

Journal: eLife

Article Title: Monoclonal antibodies derived from B cells in subjects with cystic fibrosis reduce Pseudomonas aeruginosa burden in mice

doi: 10.7554/eLife.98851

Figure Lengend Snippet:

Article Snippet: P. aeruginosa strain PA103 (ATCC 29260, sputum isolate) was grown on Difco Pseudomonas isolation agar for 16–18 hr at 37 °C and suspended in PBS.

Techniques: Transfection, Construct, Expressing, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Recombinant, Labeling, Cloning, cDNA Synthesis, Software, Concentration Assay, Buffer Exchange, Purification

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Novel humanized anti-PcrV monoclonal antibody COT-143 protects mice from lethal Pseudomonas aeruginosa infection via inhibition of toxin translocation by the type III secretion system

doi: 10.1128/aac.00694-24

Figure Lengend Snippet: in vitro cytotoxicity inhibitory activity of COT-143 against human cells. ( A ) Cytotoxicity inhibitory activity (%) of COT-143 in co-culture system of U937 (human lymphoma cell line) and P. aeruginosa PA103 is shown. The cytotoxicity was determined by the measurement of lactose dehydrogenase released from U937 cells. ( B ) Neutralization effect on the cytotoxicity inhibitory activity of COT-143 in the co-culture system is shown. COT-143 and recombinant PcrV protein (rPcrV) were mixed and pre-incubated at room temperature for more than 15 min. The rPcrV amount was 0.3, 1.0, 3.0, and 10.0-fold that of tCO-143 in molar ratio.

Article Snippet: Standard strain P. aeruginosa PA103 (ATCC29260) was purchased from American Type Culture Collection.

Techniques: In Vitro, Activity Assay, Co-Culture Assay, Neutralization, Recombinant, Incubation

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Novel humanized anti-PcrV monoclonal antibody COT-143 protects mice from lethal Pseudomonas aeruginosa infection via inhibition of toxin translocation by the type III secretion system

doi: 10.1128/aac.00694-24

Figure Lengend Snippet: Effect of COT-143 on contact-dependent hemolysis and T3SS translocon insertion. ( A ) Hemolysis levels in co-culture system of sheep red blood cells (RBCs) and P. aeruginosa PA103 with various concentrations of COT-143 are shown. Hemolysis was determined by measuring hemoglobin released from RBC. Isogenic pcrV and popBD deletion mutant of PA103 were used as a negative control. ( B ) Hemolysis inhibitory activity of COT-143 is shown. ( C and D ) Effect of COT-143 on the localization of translocon components in RBC membranes is shown. RBC membrane after the contact with PA103 with or without COT-143 (400 nM) was fractionated by sucrose-gradient ultracentrifugation, and the amount of PopB protein ( C ) and PopD protein ( D ) was determined by Western blotting using anti-PopB and anti-PopD polyclonal antibodies, respectively. Input control represents the PopB and PopD amount before the fractionation. Theoretical molecular weights of PopB and PopD are 40.1 and 31.3 kDa, respectively.

Article Snippet: Standard strain P. aeruginosa PA103 (ATCC29260) was purchased from American Type Culture Collection.

Techniques: Co-Culture Assay, Mutagenesis, Negative Control, Activity Assay, Membrane, Western Blot, Control, Fractionation

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Novel humanized anti-PcrV monoclonal antibody COT-143 protects mice from lethal Pseudomonas aeruginosa infection via inhibition of toxin translocation by the type III secretion system

doi: 10.1128/aac.00694-24

Figure Lengend Snippet: Effect of COT-143 on bacterial phagocytosis by murine neutrophil. ( A ) Viable neutrophil rate (%) in a co-culture system of murine primary neutrophils and P. aeruginosa PA103 with various concentrations of COT-143 is shown. Cytochalasin D (0.5 µg/mL) was added to inhibit phagocytosis by neutrophils. ( B ) Viable bacterial count (CFU/mL) in the co-culture system in panel A is shown.

Article Snippet: Standard strain P. aeruginosa PA103 (ATCC29260) was purchased from American Type Culture Collection.

Techniques: Co-Culture Assay