p95 pkl monoclonal antibody (Becton Dickinson)

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Becton Dickinson p95 pkl monoclonal antibody
P95 Pkl Monoclonal Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p95 pkl monoclonal antibody/product/Becton Dickinson
Average 85 stars, based on 8 article reviews
Price from $9.99 to $1999.99

p95 pkl monoclonal antibody - by Bioz Stars, 2021-01

85/100 stars

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Nucleic Acid Electrophoresis:

Article Title: Extracellular vesicles in DLBCL provide abundant clues to aberrant transcriptional programming and genomic alterations
Article Snippet: .. Protein lysates were separated by sodium dodecyl sulfateÃ¢ÂÂpolyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes, which were probed with the primary antibodies: Alix (BD 634501, at 1:500), TSG101 (abcam 125011, at 1:500), CD81 (Santa Cruz 166029, at 1:1000), and HSP90B1 (R & D 7606, at 1:3000). (For more details, see supplemental Materials and methods.) .. DLBCL cell lines (OCI-Ly1, OCI-Ly3) were pulse labeled with green fluorescent dye SYTO RNASelect (ThermoFisher) for 120 minutes at 37ÃÂ°C.

Protein Concentration:

Article Title: Suppression of canonical TGF-β signaling enables GATA4 to interact with H3K27me3 demethylase JMJD3 to promote cardiomyogenesis
Article Snippet: .. Protein concentration was determined by BCA assay and 250 µg protein lysates were incubated with anti-HA (5 µg, UBPBio Y1071), anti-Myc (2.5 µg, BD Pharmingen 551102), or anti-IgG (5 µg, Santa Cruz sc-2025) and 20 µL Protein G DynaBeads slurry (Invitrogen 10004D) overnight at 4 °C in an end-over-end mixer. .. Lysates were run on a 10% polyacrylamide gel and transferred to a PVDF membrane (110mA, 16 hours, 4 °C).

Article Title: Suppression of canonical TGF-β signaling enables GATA4 to interact with H3K27me3 demethylase JMJD3 to promote cardiomyogenesis
Article Snippet: .. Protein concentration was determined by BCA assay and 250 µg protein lysates were incubated with anti-Myc (2.5 µg, BD Pharmingen 551102) or anti-IgG (2.5 µg, Santa Cruz sc-2025) and 20 µL Protein G DynaBeads slurry (Invitrogen 10004D) overnight at 4 °C in an end-over-end mixer. .. Lysates were run on a 10% polyacrylamide gel and stained with SPYRO Ruby Protein Gel Stain (Thermo).

Generated:

Article Title: Low Concentration Microenvironments Enhance the Migration of Neonatal Cells of Glial Lineage
Article Snippet: .. Protein lysates were generated from 90 to 95% confluent serum starved cells grown in 100-mm culture plates and done in triplicate (Becton-Dickinson, MA). ..

Incubation:

Article Title: Evidence for Gardnerella vaginalis uptake and internalization by squamous vaginal epithelial cells: implications for the pathogenesis of bacterial vaginosis
Article Snippet: .. Fifteen μg of protein lysates from mock and G. vaginalis exposed cells were fractionated in 4–20% SDS-PAGE gels, transferred to nitrocellulose membranes, blocked with 5% milk, 0.1% TBST (0.1% Tween 20, 20 mM Tris, 150 mM NaCl) and incubated at 4 °C overnight with either primary monoclonal antibodies to vimentin or actin (BD Laboratories, San Diego, CA). .. Membranes were washed 5 times in washing buffer (0.1% TBST) and incubated for 1 h followed by incubation with a donkey anti-mouse peroxidase conjugate (Jackson ImmunoResearch, West Grove, PA) at a dilution of 1:10,000.

Article Title: Suppression of canonical TGF-β signaling enables GATA4 to interact with H3K27me3 demethylase JMJD3 to promote cardiomyogenesis
Article Snippet: .. Protein concentration was determined by BCA assay and 250 µg protein lysates were incubated with anti-Myc (2.5 µg, BD Pharmingen 551102) or anti-IgG (2.5 µg, Santa Cruz sc-2025) and 20 µL Protein G DynaBeads slurry (Invitrogen 10004D) overnight at 4 °C in an end-over-end mixer. .. Lysates were run on a 10% polyacrylamide gel and stained with SPYRO Ruby Protein Gel Stain (Thermo).

BIA-KA:

Article Title: Suppression of canonical TGF-β signaling enables GATA4 to interact with H3K27me3 demethylase JMJD3 to promote cardiomyogenesis
Article Snippet: .. Protein concentration was determined by BCA assay and 250 µg protein lysates were incubated with anti-Myc (2.5 µg, BD Pharmingen 551102) or anti-IgG (2.5 µg, Santa Cruz sc-2025) and 20 µL Protein G DynaBeads slurry (Invitrogen 10004D) overnight at 4 °C in an end-over-end mixer. .. Lysates were run on a 10% polyacrylamide gel and stained with SPYRO Ruby Protein Gel Stain (Thermo).

SDS Page:

Article Title: Polo-like Kinase 3 Functions as a Tumor Suppressor and Is a Negative Regulator of Hypoxia-Inducible Factor-1? under Hypoxic Conditions
Article Snippet: .. Equal amounts (50 µg) of protein lysates were analyzed by SDS-PAGE followed by immunoblotting with antibodies to Plk3 (1:1,000;BD Biosciences), HIF-1α (1:1,000;Bethyl Laboratories), HIF-1β (1:1,000;BD Biosciences), GFP (1:10,000;DAKO), Plk1 (1:1,000;Zymed), lamin B (1:1,000; Santa Cruz Biotechnology), β-actin (1:1,500;Sigma), and the HA epitope (1:1,000;Cell Signaling). .. Specific signals were detected with horseradish peroxidase–conjugated goat secondary antibodies (Cell Signaling) and enhanced chemiluminescence reagents (Pierce Biotechnology).

p95 pkl monoclonal antibody (Becton Dickinson)

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