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Affinity Biosciences nf kb p65 antibody
Nf Kb P65 Antibody, supplied by Affinity Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p65/anti+nf+%CE%BAb/pm42276472-109-0-15
Average 86 stars, based on 1 article reviews
nf kb p65 antibody - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Incubation:

Article Title: Ultra-processed foods sourced 7-ketositosterol aggravates colitis through gut dysbiosis induced-PDLIM3 activation
Article Snippet: The protein samples were boiled with a certain volume of loading buffer, loaded and separated using SDS polyacrylamide gel electrophoresis and then blotted onto a polyvinylidene fluoride (PVDF) membrane (Invitrogen, Carlsbad, CA, USA). .. The membranes were subsequently incubated overnight at 4 °C with primary antibodies against CLND−3 (rabbit anti-mouse; Affinity Biosciences), ZO-1 (rabbit anti-mouse; Proteintech Group), PDLIM3 (rabbit anti-mouse; Proteintech Group), p38 (anti-mouse monoclonal antibody, Affinity Biosciences), p -p38 (rabbit anti-mouse; Affinity Biosciences), p65 (anti-mouse monoclonal antibody, Affinity Biosciences), p -p65 (rabbit anti-mouse; Affinity Biosciences), IκBα (rabbit anti-mouse; Affinity Biosciences), and p -IκBα (rabbit anti-mouse; Affinity Biosciences) and then incubated with HRP-conjugated secondary antibodies (Beyotime Biotechnology). ..

Article Title: 5β-hydroxycostic acid from Laggera alata ameliorates sepsis-associated acute kidney injury through its anti-inflammatory and anti-ferroptosis effects via NF-κB and MAPK pathways.
Article Snippet: Ethnopharmacological relevance: The whole plant of Laggera alata is frequently utilize to remedy inflammatory diseases including nephritis as a traditional Chinese medicine.. However, its active ingredients and mechanism of action against sepsis-associated acute kidney injury (SA-AKI) are unknown.. Aim of the study: This study aimed to identify active compounds from L. alata that inhibit renal inflammation and ameliorate SA-AKI, and to elucidate their mechanisms of action.

Article Title: Ultra-processed foods sourced 7-ketositosterol aggravates colitis through gut dysbiosis induced-PDLIM3 activation.
Article Snippet: The protein samples were boiled with a certain volume of loading buffer, loaded and separated using SDS polyacrylamide gel electrophoresis and then blotted onto a polyvinylidene fluoride (PVDF) membrane (Invitrogen, Carlsbad, CA, USA). .. The membranes were subsequently incubated overnight at 4 °C with primary antibodies against CLND−3 (rabbit anti-mouse; Affinity Biosciences), ZO-1 (rabbit anti-mouse; Proteintech Group), PDLIM3 (rabbit anti-mouse; Proteintech Group), p38 (anti-mouse monoclonal antibody, Affinity Biosciences), p-p38 (rabbit antimouse; Affinity Biosciences), p65 (anti-mouse monoclonal antibody, Affinity Biosciences), p-p65 (rabbit anti-mouse; Affinity Biosciences), IκBα (rabbit anti-mouse; Affinity Biosciences), and p-IκBα (rabbit antimouse; Affinity Biosciences) and then incubated with HRP-conjugated secondary antibodies (Beyotime Biotechnology). ..

Concentration Assay:

Article Title: Gut microbiota induces mammary gland sIgA production to alleviate S. aureus mastitis.
Article Snippet: Finally, protein expression was observed using a Western blot detection system (Tanon 4500, Shanghai, China) and Chemistar High-sig ECl Western Blotting Substrate (Tanon Technology, Shanghai, China). .. The specific primary antibodies used in the experiments included p65, p-p65, IκB, p-IκB, ZO-1, Occludin, Claudin-3, 18 Cell Reports 45, 116782, January 27, 2026 p70s6k, p-p70s6k and β-actin, and were used at a dilution of 1:1000. (#AF5006, #AF2006, #AF6239, #AF6239, #AF2002, #AF5145, #DF7504, #AF0129, #AF6226, #AF3228, #AF7018, Affinity Biosciences, China). sIgA concentration determination The sIgA level in mouse milk was measured using the Mouse IgA Elisa Kit (ml037606 Enzyme-linked Biotechnology, China). ..

Article Title: Extracellular vesicles containing microbial DNA contribute to ruminal dysbiosis-induced mastitis by activating cGAS-STING-NF-κB/NLRP3 pathway
Article Snippet: .. After a 10-min block with 1 × protein-free rapid blocking solution at room temperature, specific antibodies including cGAS, STING, TBK1, p-TBK, IRF3, p-IRF3, p-p65, p65, p-IκB, IκB, ZO-1, Occludin, Claudin-3 and β-actin (Affinity Biosciences, Jiangsu, China) were used at the appropriate final concentration following the manufacturer's instructions. .. The PVDF membrane was subsequently incubated with goat anti-rabbit or rabbit anti-mouse IgG (1:20,000), washed with TBST, and detected using the ECL Plus Western blotting detection system (Tanon, Shanghai, China).

Enzyme-linked Immunosorbent Assay:

Article Title: Gut microbiota induces mammary gland sIgA production to alleviate S. aureus mastitis.
Article Snippet: Finally, protein expression was observed using a Western blot detection system (Tanon 4500, Shanghai, China) and Chemistar High-sig ECl Western Blotting Substrate (Tanon Technology, Shanghai, China). .. The specific primary antibodies used in the experiments included p65, p-p65, IκB, p-IκB, ZO-1, Occludin, Claudin-3, 18 Cell Reports 45, 116782, January 27, 2026 p70s6k, p-p70s6k and β-actin, and were used at a dilution of 1:1000. (#AF5006, #AF2006, #AF6239, #AF6239, #AF2002, #AF5145, #DF7504, #AF0129, #AF6226, #AF3228, #AF7018, Affinity Biosciences, China). sIgA concentration determination The sIgA level in mouse milk was measured using the Mouse IgA Elisa Kit (ml037606 Enzyme-linked Biotechnology, China). ..

Blocking Assay:

Article Title: Extracellular vesicles containing microbial DNA contribute to ruminal dysbiosis-induced mastitis by activating cGAS-STING-NF-κB/NLRP3 pathway
Article Snippet: .. After a 10-min block with 1 × protein-free rapid blocking solution at room temperature, specific antibodies including cGAS, STING, TBK1, p-TBK, IRF3, p-IRF3, p-p65, p65, p-IκB, IκB, ZO-1, Occludin, Claudin-3 and β-actin (Affinity Biosciences, Jiangsu, China) were used at the appropriate final concentration following the manufacturer's instructions. .. The PVDF membrane was subsequently incubated with goat anti-rabbit or rabbit anti-mouse IgG (1:20,000), washed with TBST, and detected using the ECL Plus Western blotting detection system (Tanon, Shanghai, China).



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Image Search Results


The mRNA expression of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2 , and Caspase 3 in E.tenella host cells.

Journal: Poultry Science

Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

doi: 10.1016/j.psj.2026.106922

Figure Lengend Snippet: The mRNA expression of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2 , and Caspase 3 in E.tenella host cells.

Article Snippet: p-p65 Rabbit Ab , Bioss , bs-0982R , 1: 1500.

Techniques: Expressing

The protein activity changes of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2, and Caspase 3 in E.tenella host cells.

Journal: Poultry Science

Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

doi: 10.1016/j.psj.2026.106922

Figure Lengend Snippet: The protein activity changes of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2, and Caspase 3 in E.tenella host cells.

Article Snippet: p-p65 Rabbit Ab , Bioss , bs-0982R , 1: 1500.

Techniques: Activity Assay