p4504 proteinase k  (Millipore)


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    Name:
    Potassium chloride
    Description:

    Catalog Number:
    p1597
    Price:
    None
    Applications:
    May be used for the preparation of phosphate buffered saline, and for the extraction and solubilization of proteins.
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    Structured Review

    Millipore p4504 proteinase k
    Potassium chloride

    https://www.bioz.com/result/p4504 proteinase k/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p4504 proteinase k - by Bioz Stars, 2020-04
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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: An Interaction of Rhamnolipids with Cu2+ Ions
    Article Snippet: .. The rhamnolipid R-95 (the 1:1 mixture of mono- and dirhamnolipids with the HPLC purity; the mean molecular weight of 577 g mol−1 , p K a = 5.6), methylglycinediacetic acid (MGDA; analytical grade, p K a = 14.6 [ ]), ethylenediaminetetraacetic acid (EDTA; analytical grade), potassium chloride (KCl), copper (II) sulfate (CuSO4 ; analytical grade), potassium hydroxide (KOH; analytical grade), and sulfuric acid (H2 SO4 ) were purchased from Sigma Aldrich (Poznan, Poland) company. .. Distilled filtrated (0.2 µm Whatman filters; GE Healthcare UK Ltd., Little Chalfont, UK) water with the electrolytic conductivity of 0.001 ms cm–1 at 20 ± 0.1 °C) was used for the solutions preparation.

    Article Title: An Interaction of Rhamnolipids with Cu2+ Ions
    Article Snippet: .. The Chemicals Used The rhamnolipid R-95 (the 1:1 mixture of mono- and dirhamnolipids with the HPLC purity; the mean molecular weight of 577 g mol−1 , pK a = 5.6), methylglycinediacetic acid (MGDA; analytical grade, pK a = 14.6 [ ]), ethylenediaminetetraacetic acid (EDTA; analytical grade), potassium chloride (KCl), copper (II) sulfate (CuSO4 ; analytical grade), potassium hydroxide (KOH; analytical grade), and sulfuric acid (H2 SO4 ) were purchased from Sigma Aldrich (Poznan, Poland) company. .. Distilled filtrated (0.2 µm Whatman filters; GE Healthcare UK Ltd., Little Chalfont, UK) water with the electrolytic conductivity of 0.001 ms cm–1 at 20 ± 0.1 °C) was used for the solutions preparation.

    Dominant Negative Mutation:

    Article Title: Pituitary Adenylate Cyclase-Activating Polypeptide 38-Mediated Rin Activation Requires Src and Contributes to the Regulation of Hsp27 Signaling during Neuronal Differentiation
    Article Snippet: The expression vectors for Gi2 αQ204L (QL), GqαQ209L (QL), GsαQ227L (QL), G12 αQ229L (QL), dominant-negative c-Src (DN-Src) [pUse-c-Src (K296R/Y528F)], and constitutively active c-Src (CA-Src) [pUse-c-Src (Y529F)] were kindly provided by J. H. Kehrl (National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD) ( ). .. The MEK1/2 inhibitor PD98059, the Src kinase inhibitor PP2 and its inactive analogue (PP3), 2′,5′-dideoxyadenosine (ddA), H89, the inactive cAMP analogue adenosine 3′,5′-cyclic monophosphorothioate, Rp isomer (Rp-cAMP), 8-bromo-adenosine-3′,5′-cyclic monophosphate (8-Br-cAMP), 8-( p -chlorophenylthio)-2′- O -methyl-adenosine-3′,5′-cyclic monophosphate (8-CPT-2-Me-cAMP), and cholera toxin (CTX) were purchased from Calbiochem, La Jolla, CA; the p38 MAP kinase inhibitor SB203580 was purchased from Tocris, Ellisville, MO; and PTX, A23187, potassium chloride (KCl), and forskolin were purchased from Sigma, St. Louis, MO.

    Mass Spectrometry:

    Article Title: An Interaction of Rhamnolipids with Cu2+ Ions
    Article Snippet: The rhamnolipid R-95 (the 1:1 mixture of mono- and dirhamnolipids with the HPLC purity; the mean molecular weight of 577 g mol−1 , p K a = 5.6), methylglycinediacetic acid (MGDA; analytical grade, p K a = 14.6 [ ]), ethylenediaminetetraacetic acid (EDTA; analytical grade), potassium chloride (KCl), copper (II) sulfate (CuSO4 ; analytical grade), potassium hydroxide (KOH; analytical grade), and sulfuric acid (H2 SO4 ) were purchased from Sigma Aldrich (Poznan, Poland) company. .. Distilled filtrated (0.2 µm Whatman filters; GE Healthcare UK Ltd., Little Chalfont, UK) water with the electrolytic conductivity of 0.001 ms cm–1 at 20 ± 0.1 °C) was used for the solutions preparation.

    Article Title: An Interaction of Rhamnolipids with Cu2+ Ions
    Article Snippet: The Chemicals Used The rhamnolipid R-95 (the 1:1 mixture of mono- and dirhamnolipids with the HPLC purity; the mean molecular weight of 577 g mol−1 , pK a = 5.6), methylglycinediacetic acid (MGDA; analytical grade, pK a = 14.6 [ ]), ethylenediaminetetraacetic acid (EDTA; analytical grade), potassium chloride (KCl), copper (II) sulfate (CuSO4 ; analytical grade), potassium hydroxide (KOH; analytical grade), and sulfuric acid (H2 SO4 ) were purchased from Sigma Aldrich (Poznan, Poland) company. .. Distilled filtrated (0.2 µm Whatman filters; GE Healthcare UK Ltd., Little Chalfont, UK) water with the electrolytic conductivity of 0.001 ms cm–1 at 20 ± 0.1 °C) was used for the solutions preparation.

    In Vitro:

    Article Title: The Role of Cyclooxygenase Enzymes in the Effects of Losartan and Lisinopril on the Contractions of Rat Thoracic Aorta
    Article Snippet: In this study, dipyrone (Sigma-Aldrich Inc., Germany), phenylephrine hydrochloride (Phe) (Sigma-Aldrich Inc., Germany), acetylcholine chloride (ACh) (Sigma-Aldrich Inc., Germany), potassium chloride (KCl) (Sigma-Aldrich Inc., Germany), angiotensin II (Ang II) human (Sigma-Aldrich Inc., Germany), losartan potassium (Sanovel Pharmaceuticals, Turkey), and lisinopril dihydrate (Sanovel Pharmaceuticals, Turkey) were used. .. We preferred to use lisinopril as an ACE-I because of its feature being not a pro-drug that is suitable to use for an in vitro experiment.

    Mutagenesis:

    Article Title: Pituitary Adenylate Cyclase-Activating Polypeptide 38-Mediated Rin Activation Requires Src and Contributes to the Regulation of Hsp27 Signaling during Neuronal Differentiation
    Article Snippet: Site-directed mutagenesis was used to generate Src mutants [pUse-c-Src (S17A) and pUse-CA-c-Src (S17A)]. .. The MEK1/2 inhibitor PD98059, the Src kinase inhibitor PP2 and its inactive analogue (PP3), 2′,5′-dideoxyadenosine (ddA), H89, the inactive cAMP analogue adenosine 3′,5′-cyclic monophosphorothioate, Rp isomer (Rp-cAMP), 8-bromo-adenosine-3′,5′-cyclic monophosphate (8-Br-cAMP), 8-( p -chlorophenylthio)-2′- O -methyl-adenosine-3′,5′-cyclic monophosphate (8-CPT-2-Me-cAMP), and cholera toxin (CTX) were purchased from Calbiochem, La Jolla, CA; the p38 MAP kinase inhibitor SB203580 was purchased from Tocris, Ellisville, MO; and PTX, A23187, potassium chloride (KCl), and forskolin were purchased from Sigma, St. Louis, MO.

    Protein Concentration:

    Article Title: The influence of pH and divalent/monovalent cations on the internal electron transfer (IET), enzymatic activity, and structure of fructose dehydrogenase
    Article Snippet: Chemicals D-(–)-fructose, calcium chloride (CaCl2 ), magnesium chloride hexahydrate (MgCl2 ⋅ 6 H2 O), sodium chloride (NaCl), potassium chloride (KCl), cytochrome c from bovine heart (≥95% based on MW 12,327 Da, prepared using TCA), 2,6-dichloroindophenol (DCIP), sodium acetate (CH3 COONa, NaAc), 3-(N -morpholino)propanesulfonic acid (MOPS), 2-amino-2-(hydroxymethyl)-1,3-propanediol (TRIS), sodium dihydrogen phosphate (NaH2 PO4 ), hydrochloric acid (HCl), sodium hydroxide (NaOH) were purchased from Sigma Aldrich (St. Louis, MO, USA). .. D-fructose dehydrogenase from Gluconobacter japonicus (FDH; EC 1.1.99.11) was purified from the culture supernatant of Gluconobacter japonicus NBRC 3260 obtained from the National Institute of Technology and Evaluation (Nishinomiya, Hyogo Prefecture, Japan) and solubilized in PBS buffer at pH 6 (50~500 mM) containing 0.1 mM 2-mercaptoethanol and 0.1% v/v Triton X-100 (volumetric activity measured with potassium ferricyanide at pH 4.5 = 420 ± 30 U mL−1 , specific activity = 250 ± 30 U mg-1 , protein concentration = 1.7 ± 0.2 mg mL−1 ) [ ].

    Purification:

    Article Title: The influence of pH and divalent/monovalent cations on the internal electron transfer (IET), enzymatic activity, and structure of fructose dehydrogenase
    Article Snippet: Chemicals D-(–)-fructose, calcium chloride (CaCl2 ), magnesium chloride hexahydrate (MgCl2 ⋅ 6 H2 O), sodium chloride (NaCl), potassium chloride (KCl), cytochrome c from bovine heart (≥95% based on MW 12,327 Da, prepared using TCA), 2,6-dichloroindophenol (DCIP), sodium acetate (CH3 COONa, NaAc), 3-(N -morpholino)propanesulfonic acid (MOPS), 2-amino-2-(hydroxymethyl)-1,3-propanediol (TRIS), sodium dihydrogen phosphate (NaH2 PO4 ), hydrochloric acid (HCl), sodium hydroxide (NaOH) were purchased from Sigma Aldrich (St. Louis, MO, USA). .. D-fructose dehydrogenase from Gluconobacter japonicus (FDH; EC 1.1.99.11) was purified from the culture supernatant of Gluconobacter japonicus NBRC 3260 obtained from the National Institute of Technology and Evaluation (Nishinomiya, Hyogo Prefecture, Japan) and solubilized in PBS buffer at pH 6 (50~500 mM) containing 0.1 mM 2-mercaptoethanol and 0.1% v/v Triton X-100 (volumetric activity measured with potassium ferricyanide at pH 4.5 = 420 ± 30 U mL−1 , specific activity = 250 ± 30 U mg-1 , protein concentration = 1.7 ± 0.2 mg mL−1 ) [ ].

    Article Title: DNA-Based Sensor for the Detection of an Organophosphorus Pesticide: Profenofos
    Article Snippet: Chemicals Aniline, perchloric acid (HClO4 ), tetrachloroauric acid (HAuCl4 ), sulfuric acid (H2 SO4 ), potassium ferrocyanide (K4 [Fe(CN)6 ]), potassium ferricyanide (K3 [Fe(CN)6 ]), DL-dithiothreitol (DTT), 6-mercapto-1-hexanol (MCH), di-sodium hydrogen phosphate (Na2 HPO4 ), sodium di-hydrogen phosphate di-hydrate (NaH2 PO4 ·2H2 O), profenofos, paraoxon, di-ethanolamine (DEA), potassium chloride (KCl), magnesium chloride (MgCl2 ), bovine serum albumin (BSA), streptavidin-alkaline phosphatase enzyme conjugate, 1-naphthyl-phosphate, and p -nitrophenyl phosphate were purchased from Sigma-Aldrich (Milan, Italy) at analytical grade. .. Prior to immobilisation, the thiol-modified DNA sequence was treated with dithiothreitol (DTT), purified by elution through NAP-5 columns of Sephadex G-25 DNA grade resin (GE Healthcare, Amersham Place, UK) and then quantified by measuring UV absorption at 260 nm.

    Sequencing:

    Article Title: DNA-Based Sensor for the Detection of an Organophosphorus Pesticide: Profenofos
    Article Snippet: Chemicals Aniline, perchloric acid (HClO4 ), tetrachloroauric acid (HAuCl4 ), sulfuric acid (H2 SO4 ), potassium ferrocyanide (K4 [Fe(CN)6 ]), potassium ferricyanide (K3 [Fe(CN)6 ]), DL-dithiothreitol (DTT), 6-mercapto-1-hexanol (MCH), di-sodium hydrogen phosphate (Na2 HPO4 ), sodium di-hydrogen phosphate di-hydrate (NaH2 PO4 ·2H2 O), profenofos, paraoxon, di-ethanolamine (DEA), potassium chloride (KCl), magnesium chloride (MgCl2 ), bovine serum albumin (BSA), streptavidin-alkaline phosphatase enzyme conjugate, 1-naphthyl-phosphate, and p -nitrophenyl phosphate were purchased from Sigma-Aldrich (Milan, Italy) at analytical grade. .. The DNA sequences were purchased from Eurofins Genomics (Ebersberg, Germany) and are listed below: thiol-tethered oligonucleotide complementary sequence (oligo-SH): 5′-(SH)-(CH2 )6 -CCG ATC AAG AAT CGC TGC AG-3′; biotinylated DNA aptamer (apt-BIO): 5′-(biotin)-TEG(triethylene glycol)-AAG CTT GCT TTA TAG CCT GCA GCG ATT CTT GAT CGG AAA AGG CTG AGA GCT ACG C-3′.

    Incubation:

    Article Title: Chronic β-Cell Depolarization Impairs β-Cell Identity by Disrupting a Network of Ca2+-Regulated Genes
    Article Snippet: Islet Culture Wild-type islets were incubated for 24 h in DMEM (Gibco, 11966-025) containing 5.6 mmol/L glucose, 10% FBS (Gibco, 16140–071), and 1% penicillin-streptomycin (Gibco, 15140-122). .. Experimental media contained 100 μmol/L tolbutamide (Sigma-Aldrich, T0891) or 20 mmol/L KCl (Sigma-Aldrich, P5405), with or without 50 μmol/L verapamil.

    other:

    Article Title: Ergothioneine prevents endothelial dysfunction induced by mercury chloride
    Article Snippet: Reagents EGT, potassium chloride (KCl), acetylcholine hydrochloride (ACh), phenylephrine hydrochloride (PE), serotonin hydrochloride (5-HT) and HgCl2 were obtained from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany).

    Article Title: Paraoxon Attenuates Vascular Smooth Muscle Contraction through Inhibiting Ca2+ Influx in the Rabbit Thoracic Aorta
    Article Snippet: Drugs and Chemicals Acetylcholine (ACh), sodium nitroprusside (SNP), phenylephrine (Phe), potassium chloride (KCl), N-Nitro-L-arginine (L-NNA), atropine, paraoxon (PXN), verapamil hydrochloride, nicotinamide, ryanodine, tetraethylammonium (TEA-CI), HEPES, egtazic acid, CS OH, CS CI, MgATP, and tetrodotoxin (TTX) were purchased from Sigma-Aldrich (St. Louis, MO,USA), Paraoxon was dissolved in ethanol in organ baths experiment and in DMSO in measurement of Ca2+ current experiment.

    Article Title: DNA-functionalized silicon nitride nanopores for sequence-specific recognition of DNA biosensor
    Article Snippet: Chemicals and materials 3-APTES, potassium chloride (KCl), methanol, and 1,4-phenylene diisothiocyanate were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Article Title: Label-Free Detection of Salivary Pepsin Using Gold Nanoparticle/Polypyrrole Nanocoral Modified Screen-Printed Electrode
    Article Snippet: Materials and Apparatus Pyrrole monomer (reagent grade, 98%), hydrogen tetrachloroaurate (III) trihydrate (HAuCl4 ·3H2 O), sulfuric acid (H2 SO4 ), potassium chloride (KCl), 2-naphthalenesulfonic acid (NSA), NHS, EDC, cysteamine (CA), potassium hexacyanoferrate (III) (K3 Fe(CN)6 ), potassium hexacyanoferrate (II) trihydrate (K4 Fe(CN)6 ·3H2 O), lysozyme human, α-amylase from human saliva, bovine serum albumin (BSA), and human serum albumin were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Article Title: Endothelium-independent vasorelaxant effect of 20(S)-protopanaxadiol on isolated rat thoracic aorta
    Article Snippet: Glibenclamide, propranolol, acetylcholine (ACh), barium chloride (BaCl2 ), potassium chloride (KCl), phenylephrine hydrochloride (PE), tetraethylammonium (TEA), 4-aminopyridine (4-AP), l -N-nitro arginine methyl ester ( L -NAME), methylene blue (MB), indomethacin, nifedipine and SK & F 96365 were purchased from Sigma Aldrich (St Louis, MO, USA).

    Activity Assay:

    Article Title: The influence of pH and divalent/monovalent cations on the internal electron transfer (IET), enzymatic activity, and structure of fructose dehydrogenase
    Article Snippet: Chemicals D-(–)-fructose, calcium chloride (CaCl2 ), magnesium chloride hexahydrate (MgCl2 ⋅ 6 H2 O), sodium chloride (NaCl), potassium chloride (KCl), cytochrome c from bovine heart (≥95% based on MW 12,327 Da, prepared using TCA), 2,6-dichloroindophenol (DCIP), sodium acetate (CH3 COONa, NaAc), 3-(N -morpholino)propanesulfonic acid (MOPS), 2-amino-2-(hydroxymethyl)-1,3-propanediol (TRIS), sodium dihydrogen phosphate (NaH2 PO4 ), hydrochloric acid (HCl), sodium hydroxide (NaOH) were purchased from Sigma Aldrich (St. Louis, MO, USA). .. D-fructose dehydrogenase from Gluconobacter japonicus (FDH; EC 1.1.99.11) was purified from the culture supernatant of Gluconobacter japonicus NBRC 3260 obtained from the National Institute of Technology and Evaluation (Nishinomiya, Hyogo Prefecture, Japan) and solubilized in PBS buffer at pH 6 (50~500 mM) containing 0.1 mM 2-mercaptoethanol and 0.1% v/v Triton X-100 (volumetric activity measured with potassium ferricyanide at pH 4.5 = 420 ± 30 U mL−1 , specific activity = 250 ± 30 U mg-1 , protein concentration = 1.7 ± 0.2 mg mL−1 ) [ ].

    Imaging:

    Article Title: Two Mechanisms Involved in Trigeminal CGRP Release: Implications for Migraine Treatment
    Article Snippet: The following chemicals for secretion, calcium, and sodium experiments were purchased from Sigma: potassium chloride (KCl), capsaicin, capsazepine, EGTA, and anti-goat IgG conjugated to horseradish peroxidase. .. For the calcium and sodium imaging studies, Fura-2 AM, 0.1% pluronic acid, and SBFI AM were purchased from Invitrogen (Carlsbad, CA).

    Expressing:

    Article Title: Pituitary Adenylate Cyclase-Activating Polypeptide 38-Mediated Rin Activation Requires Src and Contributes to the Regulation of Hsp27 Signaling during Neuronal Differentiation
    Article Snippet: The expression vectors for Gi2 αQ204L (QL), GqαQ209L (QL), GsαQ227L (QL), G12 αQ229L (QL), dominant-negative c-Src (DN-Src) [pUse-c-Src (K296R/Y528F)], and constitutively active c-Src (CA-Src) [pUse-c-Src (Y529F)] were kindly provided by J. H. Kehrl (National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD) ( ). .. The MEK1/2 inhibitor PD98059, the Src kinase inhibitor PP2 and its inactive analogue (PP3), 2′,5′-dideoxyadenosine (ddA), H89, the inactive cAMP analogue adenosine 3′,5′-cyclic monophosphorothioate, Rp isomer (Rp-cAMP), 8-bromo-adenosine-3′,5′-cyclic monophosphate (8-Br-cAMP), 8-( p -chlorophenylthio)-2′- O -methyl-adenosine-3′,5′-cyclic monophosphate (8-CPT-2-Me-cAMP), and cholera toxin (CTX) were purchased from Calbiochem, La Jolla, CA; the p38 MAP kinase inhibitor SB203580 was purchased from Tocris, Ellisville, MO; and PTX, A23187, potassium chloride (KCl), and forskolin were purchased from Sigma, St. Louis, MO.

    BIA-KA:

    Article Title: Concentration dependent effect of calcium on brain mitochondrial bioenergetics and oxidative stress parameters
    Article Snippet: Reagents Mannitol, sucrose, bovine serum albumin (BSA), ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA), hydroxyethyl piperazine-1-ethanesulfonic acid potassium salt (HEPES), potassium phosphate monobasic anhydrous (KH2 PO4 ), magnesium chloride (MgCl2 ), malate, pyruvate, adenosine 5′-diphosphate (ADP), succinate, calcium chloride (CaCl2 ), L-Arginine (L-Arg), potassium chloride (KCl), and horseradish peroxidase (HRP) were purchased from Sigma-Aldrich (St. Louis, MO, USA). .. Bicinchoninic acid (BCA) protein assay kit was purchased from Pierce (Rockford, IL).

    CTG Assay:

    Article Title: DNA-Based Sensor for the Detection of an Organophosphorus Pesticide: Profenofos
    Article Snippet: Chemicals Aniline, perchloric acid (HClO4 ), tetrachloroauric acid (HAuCl4 ), sulfuric acid (H2 SO4 ), potassium ferrocyanide (K4 [Fe(CN)6 ]), potassium ferricyanide (K3 [Fe(CN)6 ]), DL-dithiothreitol (DTT), 6-mercapto-1-hexanol (MCH), di-sodium hydrogen phosphate (Na2 HPO4 ), sodium di-hydrogen phosphate di-hydrate (NaH2 PO4 ·2H2 O), profenofos, paraoxon, di-ethanolamine (DEA), potassium chloride (KCl), magnesium chloride (MgCl2 ), bovine serum albumin (BSA), streptavidin-alkaline phosphatase enzyme conjugate, 1-naphthyl-phosphate, and p -nitrophenyl phosphate were purchased from Sigma-Aldrich (Milan, Italy) at analytical grade. .. The DNA sequences were purchased from Eurofins Genomics (Ebersberg, Germany) and are listed below: thiol-tethered oligonucleotide complementary sequence (oligo-SH): 5′-(SH)-(CH2 )6 -CCG ATC AAG AAT CGC TGC AG-3′; biotinylated DNA aptamer (apt-BIO): 5′-(biotin)-TEG(triethylene glycol)-AAG CTT GCT TTA TAG CCT GCA GCG ATT CTT GAT CGG AAA AGG CTG AGA GCT ACG C-3′.

    Molecular Weight:

    Article Title: All-Solid-State Sodium-Selective Electrode with a Solid Contact of Chitosan/Prussian Blue Nanocomposite
    Article Snippet: .. Reagents and Instruments Chitosan (low molecular weight), potassium ferricyanide (K3 [Fe(CN)]6 ), ferrous chloride (FeCl2 ), sodium chloride (NaCl), potassium chloride (KCl), tetrahydrofuran (THF), bis[(12-crown-4)methyl]dodecylmethylmalonate (sodium Ionophore VI; Selectophore grade), 2-nitrophenyl octyl ether (2-NPOE), sodium tetraphenylborate (NaTPB) and polyvinyl butyral (PBV, Butvar® B-98) were purchased from Sigma-Aldrich (Darmstadt, Germany). .. Acetic acid (Glacial, 98%), acetone, glutaraldehyde, Na2 HPO4 , NaH2 PO4 , NH4 Cl, NH4 OH and potassium phosphate buffer (50 mM, pH 7.4) were purchased from Fisher Scientific (Waltham, MA, USA).

    Article Title: An Interaction of Rhamnolipids with Cu2+ Ions
    Article Snippet: .. The rhamnolipid R-95 (the 1:1 mixture of mono- and dirhamnolipids with the HPLC purity; the mean molecular weight of 577 g mol−1 , p K a = 5.6), methylglycinediacetic acid (MGDA; analytical grade, p K a = 14.6 [ ]), ethylenediaminetetraacetic acid (EDTA; analytical grade), potassium chloride (KCl), copper (II) sulfate (CuSO4 ; analytical grade), potassium hydroxide (KOH; analytical grade), and sulfuric acid (H2 SO4 ) were purchased from Sigma Aldrich (Poznan, Poland) company. .. Distilled filtrated (0.2 µm Whatman filters; GE Healthcare UK Ltd., Little Chalfont, UK) water with the electrolytic conductivity of 0.001 ms cm–1 at 20 ± 0.1 °C) was used for the solutions preparation.

    Article Title: An Interaction of Rhamnolipids with Cu2+ Ions
    Article Snippet: .. The Chemicals Used The rhamnolipid R-95 (the 1:1 mixture of mono- and dirhamnolipids with the HPLC purity; the mean molecular weight of 577 g mol−1 , pK a = 5.6), methylglycinediacetic acid (MGDA; analytical grade, pK a = 14.6 [ ]), ethylenediaminetetraacetic acid (EDTA; analytical grade), potassium chloride (KCl), copper (II) sulfate (CuSO4 ; analytical grade), potassium hydroxide (KOH; analytical grade), and sulfuric acid (H2 SO4 ) were purchased from Sigma Aldrich (Poznan, Poland) company. .. Distilled filtrated (0.2 µm Whatman filters; GE Healthcare UK Ltd., Little Chalfont, UK) water with the electrolytic conductivity of 0.001 ms cm–1 at 20 ± 0.1 °C) was used for the solutions preparation.

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