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Altered receptor expression in mediating diminished BSM contractility upon pressure treatment. A, Western blot images of M2, M3, and <t>P2X1</t> receptor protein expression in mouse bladders from control (n = 4) and pressure treated mice (50 cm: n = 4 for 0 hours, 6 hours, and 24 hours, respectively. 80 cm, n = 4 for 0 hours, 6 hours, and 24 hours, respectively). B-D, are quantitated data by densitometry and normalized to actin. Data are shown as box and whiskers, center line is the median of the data set, box represents 75% of the data, and bars indicate whiskers from minimum to maximum. Data were analyzed by Student’s t test between control group and 50 or 80 cm H2O pressure treated groups. *P < .05, **P < .001
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P2 receptors (P2X 1 , P2X 4 , P2X 7 , P2Y 1 , and P2Y 11 ) compared by Western blotting in B cells and LCLs . Proteins extracted from B cells and LCLs were probed with rabbit <t>polyclonal</t> antibodies directed against P2X 1 (60-kDa), P2X 4 (65-kDa), P2X 7 (68-kDa), P2Y 1 (66-kDa), and P2Y 11 (50-kDa) (left). Membranes were re-probed by GAPDH (40-kDa) (right). Data are representatives of 4 independent experiments.
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Primary antibodies used in this study
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Primary antibodies used in this study
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Primary antibodies used in this study
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Altered receptor expression in mediating diminished BSM contractility upon pressure treatment. A, Western blot images of M2, M3, and P2X1 receptor protein expression in mouse bladders from control (n = 4) and pressure treated mice (50 cm: n = 4 for 0 hours, 6 hours, and 24 hours, respectively. 80 cm, n = 4 for 0 hours, 6 hours, and 24 hours, respectively). B-D, are quantitated data by densitometry and normalized to actin. Data are shown as box and whiskers, center line is the median of the data set, box represents 75% of the data, and bars indicate whiskers from minimum to maximum. Data were analyzed by Student’s t test between control group and 50 or 80 cm H2O pressure treated groups. *P < .05, **P < .001

Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Article Title: Molecular mechanisms of voiding dysfunction in a novel mouse model of acute urinary retention

doi: 10.1096/fj.202002415R

Figure Lengend Snippet: Altered receptor expression in mediating diminished BSM contractility upon pressure treatment. A, Western blot images of M2, M3, and P2X1 receptor protein expression in mouse bladders from control (n = 4) and pressure treated mice (50 cm: n = 4 for 0 hours, 6 hours, and 24 hours, respectively. 80 cm, n = 4 for 0 hours, 6 hours, and 24 hours, respectively). B-D, are quantitated data by densitometry and normalized to actin. Data are shown as box and whiskers, center line is the median of the data set, box represents 75% of the data, and bars indicate whiskers from minimum to maximum. Data were analyzed by Student’s t test between control group and 50 or 80 cm H2O pressure treated groups. *P < .05, **P < .001

Article Snippet: Western blot analysis Rabbit anti-P2X1 antibody (1:1000, #APR-001) and rabbit anti-chrm2 antibody (1:1000, #AMR-002) were purchased from Alomone Lab. Rabbit anti-chrm3 antibody (1:1000, #PA5–77485) was purchased from Thermo Fisher Scientific.

Techniques: Expressing, Western Blot, Control

P2 receptors (P2X 1 , P2X 4 , P2X 7 , P2Y 1 , and P2Y 11 ) compared by Western blotting in B cells and LCLs . Proteins extracted from B cells and LCLs were probed with rabbit polyclonal antibodies directed against P2X 1 (60-kDa), P2X 4 (65-kDa), P2X 7 (68-kDa), P2Y 1 (66-kDa), and P2Y 11 (50-kDa) (left). Membranes were re-probed by GAPDH (40-kDa) (right). Data are representatives of 4 independent experiments.

Journal: BMC Immunology

Article Title: Expression of P2 receptors in human B cells and Epstein-Barr virus-transformed lymphoblastoid cell lines

doi: 10.1186/1471-2172-7-22

Figure Lengend Snippet: P2 receptors (P2X 1 , P2X 4 , P2X 7 , P2Y 1 , and P2Y 11 ) compared by Western blotting in B cells and LCLs . Proteins extracted from B cells and LCLs were probed with rabbit polyclonal antibodies directed against P2X 1 (60-kDa), P2X 4 (65-kDa), P2X 7 (68-kDa), P2Y 1 (66-kDa), and P2Y 11 (50-kDa) (left). Membranes were re-probed by GAPDH (40-kDa) (right). Data are representatives of 4 independent experiments.

Article Snippet: The membrane was incubated with rabbit polyclonal antibodies (Alomone Labs, Jerusalem, Israel) against P2X 1 receptor, P2X 4 receptor, P2X 7 receptor, P2Y 1 receptor, or P2Y 11 receptor in TBST for 2 hours at room temperature, washed with TBST, and incubated with secondary anti-rabbit IgG (Amersham Pharmacia Biotech) in TBST for 1 hour.

Techniques: Western Blot

Primary antibodies used in this study

Journal: Purinergic Signalling

Article Title: Expression of P2X receptors in the rat anterior pituitary

doi: 10.1007/s11302-019-09685-y

Figure Lengend Snippet: Primary antibodies used in this study

Article Snippet: Table shows the primary antibodies used in this study. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Primary antibodies Host company Catalog no. Dilution IF TSA WB P2X1 Rabbit Alomone APR-001 1:200 1:4000 1:1000 P2X2 Rabbit Alomone APR-003 1:200 1:4000 1:1000 P2X3 Rabbit Roche 1:400 1:8000 1:1000 P2X4 Rabbit Santa Cruz ARP-002 1:400 1:8000 1:1000 P2X5 Rabbit Boster PB0339 1:500 1:8000 1:1000 P2X6 Rabbit Alomone APR-013 1:200 1:4000 1:1000 P2X7 Rabbit Alomone APR-004 1:400 1:8000 1:1000 ACTH Rabbit Sigma A1927 1:600 1:10000 TSH Rabbit Chemicon AB976 1:400 1:10000 S-100 Mouse Abcam ab4066 1:200 Iba-1 Rabbit Wako 17919741 1:1000 Digoxigenin Mouse Jackson 200–002-156 1:200 GAPDH Mouse Beyotime AG019 1:1000 Open in a separate window Primary antibodies used in this study As most of the primary antibodies are from the same host (rabbit), the double- or triple-labeling immunofluorescence protocol was used and modified based on previous reports [ 11 , 15 ].

Techniques:

Western blot analysis of P2X receptor expression in the anterior pituitary. a Lane P2X1 to P2X7 are the results detected by P2X1 to P2X7 antibodies, respectively, lane M is the molecular weight marker, note that a strong immunostained band was detected by the P2X4 antibody, followed by the P2X5, P2X3, P2X2, P2X7, and P2X6 receptor antibodies, no band was detected by P2X1 receptor antibody. b Lane P2X1 to P2X7 are the results of the antibody pre-absorption control experiments for P2X1 to P2X7, note that no bands were detected. c The band density ratio of each P2X receptor over GAPDH

Journal: Purinergic Signalling

Article Title: Expression of P2X receptors in the rat anterior pituitary

doi: 10.1007/s11302-019-09685-y

Figure Lengend Snippet: Western blot analysis of P2X receptor expression in the anterior pituitary. a Lane P2X1 to P2X7 are the results detected by P2X1 to P2X7 antibodies, respectively, lane M is the molecular weight marker, note that a strong immunostained band was detected by the P2X4 antibody, followed by the P2X5, P2X3, P2X2, P2X7, and P2X6 receptor antibodies, no band was detected by P2X1 receptor antibody. b Lane P2X1 to P2X7 are the results of the antibody pre-absorption control experiments for P2X1 to P2X7, note that no bands were detected. c The band density ratio of each P2X receptor over GAPDH

Article Snippet: Table shows the primary antibodies used in this study. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Primary antibodies Host company Catalog no. Dilution IF TSA WB P2X1 Rabbit Alomone APR-001 1:200 1:4000 1:1000 P2X2 Rabbit Alomone APR-003 1:200 1:4000 1:1000 P2X3 Rabbit Roche 1:400 1:8000 1:1000 P2X4 Rabbit Santa Cruz ARP-002 1:400 1:8000 1:1000 P2X5 Rabbit Boster PB0339 1:500 1:8000 1:1000 P2X6 Rabbit Alomone APR-013 1:200 1:4000 1:1000 P2X7 Rabbit Alomone APR-004 1:400 1:8000 1:1000 ACTH Rabbit Sigma A1927 1:600 1:10000 TSH Rabbit Chemicon AB976 1:400 1:10000 S-100 Mouse Abcam ab4066 1:200 Iba-1 Rabbit Wako 17919741 1:1000 Digoxigenin Mouse Jackson 200–002-156 1:200 GAPDH Mouse Beyotime AG019 1:1000 Open in a separate window Primary antibodies used in this study As most of the primary antibodies are from the same host (rabbit), the double- or triple-labeling immunofluorescence protocol was used and modified based on previous reports [ 11 , 15 ].

Techniques: Western Blot, Expressing, Molecular Weight, Marker

Expression of P2X1 to P2X7 receptor-ir (red) in the rat anterior pituitary. a to f P2X1 to P2X7 receptor immunoreactive cells respectively. Note that strong immunostaining was detected using the P2X4 receptor antibody, followed by P2X5, P2X3, P2X6, P2X2, and P2X7 receptor antibodies. No obvious immunostaining was detected by the P2X1 antibody. g A merged image from f and an image of Iba-1-ir cells (green) (a macrophage/microglia marker) in the same field of f. Note that all the P2X7 receptor reactive cells were labeled by Iba-1-ir. All scale bars = 120 μm

Journal: Purinergic Signalling

Article Title: Expression of P2X receptors in the rat anterior pituitary

doi: 10.1007/s11302-019-09685-y

Figure Lengend Snippet: Expression of P2X1 to P2X7 receptor-ir (red) in the rat anterior pituitary. a to f P2X1 to P2X7 receptor immunoreactive cells respectively. Note that strong immunostaining was detected using the P2X4 receptor antibody, followed by P2X5, P2X3, P2X6, P2X2, and P2X7 receptor antibodies. No obvious immunostaining was detected by the P2X1 antibody. g A merged image from f and an image of Iba-1-ir cells (green) (a macrophage/microglia marker) in the same field of f. Note that all the P2X7 receptor reactive cells were labeled by Iba-1-ir. All scale bars = 120 μm

Article Snippet: Table shows the primary antibodies used in this study. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Primary antibodies Host company Catalog no. Dilution IF TSA WB P2X1 Rabbit Alomone APR-001 1:200 1:4000 1:1000 P2X2 Rabbit Alomone APR-003 1:200 1:4000 1:1000 P2X3 Rabbit Roche 1:400 1:8000 1:1000 P2X4 Rabbit Santa Cruz ARP-002 1:400 1:8000 1:1000 P2X5 Rabbit Boster PB0339 1:500 1:8000 1:1000 P2X6 Rabbit Alomone APR-013 1:200 1:4000 1:1000 P2X7 Rabbit Alomone APR-004 1:400 1:8000 1:1000 ACTH Rabbit Sigma A1927 1:600 1:10000 TSH Rabbit Chemicon AB976 1:400 1:10000 S-100 Mouse Abcam ab4066 1:200 Iba-1 Rabbit Wako 17919741 1:1000 Digoxigenin Mouse Jackson 200–002-156 1:200 GAPDH Mouse Beyotime AG019 1:1000 Open in a separate window Primary antibodies used in this study As most of the primary antibodies are from the same host (rabbit), the double- or triple-labeling immunofluorescence protocol was used and modified based on previous reports [ 11 , 15 ].

Techniques: Expressing, Immunostaining, Marker, Labeling

Coexistence between P2X receptors and pituitary hormones or S100

Journal: Purinergic Signalling

Article Title: Expression of P2X receptors in the rat anterior pituitary

doi: 10.1007/s11302-019-09685-y

Figure Lengend Snippet: Coexistence between P2X receptors and pituitary hormones or S100

Article Snippet: Table shows the primary antibodies used in this study. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Primary antibodies Host company Catalog no. Dilution IF TSA WB P2X1 Rabbit Alomone APR-001 1:200 1:4000 1:1000 P2X2 Rabbit Alomone APR-003 1:200 1:4000 1:1000 P2X3 Rabbit Roche 1:400 1:8000 1:1000 P2X4 Rabbit Santa Cruz ARP-002 1:400 1:8000 1:1000 P2X5 Rabbit Boster PB0339 1:500 1:8000 1:1000 P2X6 Rabbit Alomone APR-013 1:200 1:4000 1:1000 P2X7 Rabbit Alomone APR-004 1:400 1:8000 1:1000 ACTH Rabbit Sigma A1927 1:600 1:10000 TSH Rabbit Chemicon AB976 1:400 1:10000 S-100 Mouse Abcam ab4066 1:200 Iba-1 Rabbit Wako 17919741 1:1000 Digoxigenin Mouse Jackson 200–002-156 1:200 GAPDH Mouse Beyotime AG019 1:1000 Open in a separate window Primary antibodies used in this study As most of the primary antibodies are from the same host (rabbit), the double- or triple-labeling immunofluorescence protocol was used and modified based on previous reports [ 11 , 15 ].

Techniques: