Journal: Clinical and Molecular Hepatology

Article Title: Modulation of phosphatase of regenerating liver-1 within placental mesenchymal stem cells instigates the transition between epithelial-to-mesenchymal transition and mesenchymal-to-epithelial transition subsequent to hepatic fibrosis

doi: 10.3350/cmh.2024.0741

Figure Lengend Snippet: Placenta-derived mesenchymal stem cells engineered to overexpress phosphatase of regenerating liver-1 (PD-MSCs PRL-1 ) regulates cell survival and death in BDL rats. (A) Volcano plot showing upregulated and downregulated factors in the supernatant of PD-MSCs and PD-MSCs PRL-1 . (B) Serum interleukin (IL)-6 levels quantified by ELISA (n=18–21/group). (C) Western blotting of liver regeneration markers (IL-6, GP130, t/p-STAT3, ALB) and correlation between IL-6 and p-STAT3 (n=4–5/group). Serum albumin levels (n=5–18/group). (D) Western blotting of antiapoptotic (BCL2) and proapoptotic (BAX, c-PARP, c-CASPASE-3) markers in bile duct ligation (BDL) rat liver protein. Quantification of c-CASPASE-3 (n=5–18/group). (E) Western blotting of cell survival markers (CDK4, CCND1) in BDL rat liver protein (n=5/group), normalized to GAPDH. (F) IHC images stained by PCNA and quantification of hepatocyte nuclei (strong: dark blue, moderate: regular blue, weak: light blue) (n=4–5/group). Scale bar=100 μm. Value represents mean±standard deviation, as determined by one-way ANOVA. ns, not significant. * P <0.05, ** P <0.01, **** P <0.0001.

Article Snippet: Primary antibodies against ALB, BCL2, COL1A1 (Novus Biologicals), ACTA2 (Sigma-Aldrich), BAX, VIM, SNAI1, p-SMAD1/5, GP130 (Santa Cruz Biotechnology), PARP, total/p-SMAD2, TJP, CDH1, total/p-STAT3, interleukin (IL)-6, CDK4, CCND1, CASPASE-3 (Cell Signaling Technology, Danvers, MA, USA), PRL-1, BMP7, and total SMAD1/5 (Abcam) were used.

Techniques: Derivative Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Ligation, Staining, Standard Deviation