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Journal: Bioactive Materials
Article Title: Mitochondrial-targeted injectable hydrogel for periodontitis therapy via oral immunity and flora regulation
doi: 10.1016/j.bioactmat.2025.12.002
Figure Lengend Snippet: Study on antibacterial and antibiofilm activity, and mechanism of hydrogels in vitro . (A) Representative images of the colonies of S. aureus , MRSA , E. coli and P. gingivalis on LB agar plates and (B, C) their relative bacterial viability in different groups (n = 3). (D) SEM images of MRSA and P. gingivalis . (E) Live (green)/dead (red) staining of MRSA and P. gingivalis , and (F) corresponding quantitative analysis of the live/dead ratio (n = 3). (G) The diagram of antimicrobial mechanism of melittin. (H) Crystal violet staining of the bacterial biofilms and corresponding quantitative analysis of (I, J) biofilm formation and (K, L) biofilm reduction in different groups (n = 3). (M) Membrane permeabilizing and (N) depolarization effect of hydrogel against MRSA and P. ginivalis . (O) Protein leakage in MRSA and P. ginivalis after hydrogels treatment (n = 3). (P) Metabolic activity of MRSA and P. ginivalis under different treatments (n = 3). ∗∗ p < 0.01, ∗∗∗ p < 0.001.
Article Snippet: The antibacterial efficacy of hydrogels was evaluated using S. aureus (ATCC 6538),
Techniques: Activity Assay, In Vitro, Staining, Membrane