braf p v600e mutant  (ATCC)

Journal: Journal of Hematopathology

Article Title: BRAF V600E mutation and high expression of PD-L1 in Rosai-Dorfman disease: case report and review of the literature

doi: 10.1007/s12308-024-00611-9

Figure Lengend Snippet: Histopathological and immunohistochemistry characteristics of the case. A H&E stain showing histiocytes with emperipolesis. B Immunohistochemistry analysis revealing atypical histiocytes positive for CD163. C Immunohistochemistry with VE1 showing atypical histiocytes containing the mutant BRAF V600E protein. D RDD histiocyte (inset) showing PD-L1 membranous staining (2 +) observed in 25–30% of histiocytes

Article Snippet: D RDD histiocyte (inset) showing PD-L1 membranous staining (2 +) observed in 25–30% of histiocytes Fig. 2 Foundation Medicine NGS sequencing data showing the presence of a BRAF (NM_030621.3) variant: p V600E (c.1799 T > A) at 1.7% variant allele frequency The patient subsequently underwent 18F-FDG PET/CT (including contrast-enhanced companion CT) which showed hypermetabolic activity in the bilateral internal jugular and right submandibular and left axillary lymph nodes as well as a hypermetabolic lytic lesion in the right T8 vertebral body with adjacent right paraspinal soft tissue hypermetabolic activity.

Techniques: Immunohistochemistry, Staining, Mutagenesis

Journal: Journal of Hematopathology

Article Title: BRAF V600E mutation and high expression of PD-L1 in Rosai-Dorfman disease: case report and review of the literature

doi: 10.1007/s12308-024-00611-9

Figure Lengend Snippet: Foundation Medicine NGS sequencing data showing the presence of a BRAF (NM_030621.3) variant: p V600E (c.1799 T > A) at 1.7% variant allele frequency

Article Snippet: D RDD histiocyte (inset) showing PD-L1 membranous staining (2 +) observed in 25–30% of histiocytes Fig. 2 Foundation Medicine NGS sequencing data showing the presence of a BRAF (NM_030621.3) variant: p V600E (c.1799 T > A) at 1.7% variant allele frequency The patient subsequently underwent 18F-FDG PET/CT (including contrast-enhanced companion CT) which showed hypermetabolic activity in the bilateral internal jugular and right submandibular and left axillary lymph nodes as well as a hypermetabolic lytic lesion in the right T8 vertebral body with adjacent right paraspinal soft tissue hypermetabolic activity.

Techniques: Sequencing, Variant Assay

Journal: Journal of Hematopathology

Article Title: BRAF V600E mutation and high expression of PD-L1 in Rosai-Dorfman disease: case report and review of the literature

doi: 10.1007/s12308-024-00611-9

Figure Lengend Snippet: Reported BRAF-Mutated RDD or mixed histiocytosis with emperipolesis

Article Snippet: D RDD histiocyte (inset) showing PD-L1 membranous staining (2 +) observed in 25–30% of histiocytes Fig. 2 Foundation Medicine NGS sequencing data showing the presence of a BRAF (NM_030621.3) variant: p V600E (c.1799 T > A) at 1.7% variant allele frequency The patient subsequently underwent 18F-FDG PET/CT (including contrast-enhanced companion CT) which showed hypermetabolic activity in the bilateral internal jugular and right submandibular and left axillary lymph nodes as well as a hypermetabolic lytic lesion in the right T8 vertebral body with adjacent right paraspinal soft tissue hypermetabolic activity.

Techniques: Mutagenesis, Sequencing, Imaging, Multiplex Assay, Digital PCR