bacterial host strain p syringae pv  (DSMZ)

Journal: PLoS ONE

Article Title: Patterning Bacterial Communities on Epithelial Cells

doi: 10.1371/journal.pone.0067165

Figure Lengend Snippet: The three preys; E. coli MG1655, S. boydii KACC 10792, and P . sp DSM 50906 were suspended in the DEX rich phase and spotted as separate drops in the same plate at an initial rOD of 0.5. For the predator plates, B . bacteriovorus HD 100 was added to the PEG phase as described in the Materials and Methods section. The control plates were filled with only the PEG rich phase, i.e., without the predator added. Both sets of plates were incubated for 24 h then observed microscopically. A) The confocal microscopy photos for the three preys communities in both the control plate and the predated ( B . bacteriovorus HD 100 containing) one. All images in panels A were taken using the same microscopy settings so that the data for the three organisms could be compared. Scale bar: 1mm. B ) ImageJ analysis for the images in panel (A) showing the percent area of the image fluorescing red. The error bars show the standard error obtained from the analysis of at least 3 images for each case. Statistical analysis was performed using t-test. * p < 0.05, ** p < 0.01, *** p < 0.001. C) B . bacteriovorus predation protected MCF 10a against the effects of P . sp DSM 50906. P . sp was spotted at a rOD of 5. After incubating the plates for 24 h at 37°C, the epithelial cells were stained and observed using an epifluorescence microscope. The plot compares the green fluorescence of the epithelial areas underneath the P . sp spots for both the control and predated plates. The error bars represent the standard error of six replicates for each case. ** p < 0.01. The images are representative photos showing the appearance of the two areas as observed using epiflourescence microscope. Scale bar: 500µm.

Article Snippet: The strains used in this study were E. coli MG1655, and S. boydii KACC 10792, which were obtained from the Korean RDA-Genebank Information Center (genebank.rda.go.kr), as well as P. sp DSM 50906 and B . bacteriovorus HD 100, both of which were purchased from the German Collection of Microorganisms and Cell Cultures (DSMZ).

Techniques: Incubation, Confocal Microscopy, Microscopy, Staining, Fluorescence

Journal: Microorganisms

Article Title: Efficiency of Phage φ6 for Biocontrol of Pseudomonas syringae pv. syringae : An in Vitro Preliminary Study

doi: 10.3390/microorganisms7090286

Figure Lengend Snippet: Host range of phage φ6 determined on 25 bacterial strains. Clear lysis zone (+) and not lysis zone (−). The plating with the host strain was considered as EOP = 100%. EOP: Efficiency of Plating.

Article Snippet: The bacterial strain P. syringae pv. syringae (DSM 21482) was purchased from Leibniz-Institute DSMZ—Deutsche Sammlung von Mikroorganismen und Zellkulturen GmmH (Braunschweig, Germany).

Techniques: Lysis, Spot Test

Journal: Microorganisms

Article Title: Efficiency of Phage φ6 for Biocontrol of Pseudomonas syringae pv. syringae : An in Vitro Preliminary Study

doi: 10.3390/microorganisms7090286

Figure Lengend Snippet: One-step growth curve of phage φ6 in the presence of P. syringae pv. syringae as the host. Values represent the mean of three experiments; Error bars represent the standard deviation.

Article Snippet: The bacterial strain P. syringae pv. syringae (DSM 21482) was purchased from Leibniz-Institute DSMZ—Deutsche Sammlung von Mikroorganismen und Zellkulturen GmmH (Braunschweig, Germany).

Techniques: Standard Deviation

Journal: Microorganisms

Article Title: Efficiency of Phage φ6 for Biocontrol of Pseudomonas syringae pv. syringae : An in Vitro Preliminary Study

doi: 10.3390/microorganisms7090286

Figure Lengend Snippet: Adsorption curve of phage φ6 particles onto P. syringae pv. syringae host cells, allowing calculation of the phage particles’ adsorption rate following non-linear fitting of a logarithmic function to the experimental data. Error bars represent the standard deviation

Article Snippet: The bacterial strain P. syringae pv. syringae (DSM 21482) was purchased from Leibniz-Institute DSMZ—Deutsche Sammlung von Mikroorganismen und Zellkulturen GmmH (Braunschweig, Germany).

Techniques: Adsorption, Standard Deviation

Journal: Microorganisms

Article Title: Efficiency of Phage φ6 for Biocontrol of Pseudomonas syringae pv. syringae : An in Vitro Preliminary Study

doi: 10.3390/microorganisms7090286

Figure Lengend Snippet: Inactivation of P. syringae pv. syringae by phage φ6 at a multiplicity of infection (MOI) of 1 and 100 during 24 h. ( a ) Bacterial concentration: BC, bacteria control; BP, bacteria plus phage; ( b ) Phage concentration: PC, phage control; BP, bacteria plus phage; ( c ) Percentage of phage sensitivity of surviving bacteria after phage exposure of P. syringae pv. syringae cells sensitive to phage φ6 at a MOI of 1, after 0, 6, 12, 18, and 24 h of incubation, following spot testing of randomly chosen bacterial colonies. Values represent the mean of three independent assays; Error bars represent the standard deviation.

Article Snippet: The bacterial strain P. syringae pv. syringae (DSM 21482) was purchased from Leibniz-Institute DSMZ—Deutsche Sammlung von Mikroorganismen und Zellkulturen GmmH (Braunschweig, Germany).

Techniques: Infection, Concentration Assay, Incubation, Standard Deviation

Journal: Microorganisms

Article Title: Efficiency of Phage φ6 for Biocontrol of Pseudomonas syringae pv. syringae : An in Vitro Preliminary Study

doi: 10.3390/microorganisms7090286

Figure Lengend Snippet: Frequency of emergence of P. syringae pv. syringae spontaneous phage-resistant mutants.

Article Snippet: The bacterial strain P. syringae pv. syringae (DSM 21482) was purchased from Leibniz-Institute DSMZ—Deutsche Sammlung von Mikroorganismen und Zellkulturen GmmH (Braunschweig, Germany).

Techniques:

Journal: Microorganisms

Article Title: Efficiency of Phage φ6 for Biocontrol of Pseudomonas syringae pv. syringae : An in Vitro Preliminary Study

doi: 10.3390/microorganisms7090286

Figure Lengend Snippet: P. syringae pv. syringae concentration of resistant mutants versus their sensitive cells in the presence or absence of phage φ6 at a multiplicity of infection (MOI) of 1 after 6, 12, 18, and 24 h of incubation. Values represent the mean of three independent assays; error bars represent the standard deviation.

Article Snippet: The bacterial strain P. syringae pv. syringae (DSM 21482) was purchased from Leibniz-Institute DSMZ—Deutsche Sammlung von Mikroorganismen und Zellkulturen GmmH (Braunschweig, Germany).

Techniques: Concentration Assay, Infection, Incubation, Standard Deviation

Journal: Transgenic Research

Article Title: Safety of Pseudomonas chlororaphis as a gene source for genetically modified crops

doi: 10.1007/s11248-018-0061-6

Figure Lengend Snippet: Biopesticide products and genetically modified (GM) crops utilizing Pseudomonas spp. (Only naturally occurring strains of Pseudomonas spp. are reported) or a related species as the donor source

Article Snippet: Pseudomonas sp . strain DSMZ 13134; closely related to P . fluorescens ) , 2012 (EFSA) , Proradix ® , Biopesticide—protection against fungal diseases in vegetables and flowers (Buddrus-Schiemann et al. ; EFSA ) .

Techniques: Genetically Modified, Recombinant