Journal: BMC Microbiology

Article Title: Cytotoxicity and inflammatory potential of two Pseudomonas mosselii strains isolated from clinical samples of hospitalized patients

doi: 10.1186/1471-2180-13-123

Figure Lengend Snippet: Cytotoxic effects of P. mosselii ATCC BAA-99, P. mosselii MFY161 and P. aeruginosa PAO1 on Caco-2/TC7 cells. Cytotoxicity was determined by LDH release assay after 24 h of infection. Results were calculated as the mean values (+/−SEM) of three independent experiments. *** P < 0.001 versus uninfected Caco-2/TC7 cells, ∆∆∆ P < 0.001 versus P. aeruginosa PAO1 , ∆∆ P < 0.01 versus P. aeruginosa PAO1, •• P < 0.01 versus P. mosselii ATCC BAA-99.

Article Snippet: P. mosselii ATCC BAA-99 is a clinical strain isolated from tracheal aspirate of a patient suffering from pulmonary infections [ ].

Techniques: Lactate Dehydrogenase Assay, Infection

Journal: BMC Microbiology

Article Title: Cytotoxicity and inflammatory potential of two Pseudomonas mosselii strains isolated from clinical samples of hospitalized patients

doi: 10.1186/1471-2180-13-123

Figure Lengend Snippet: Invasive capacity of P. mosselii ATCC BAA-99, P. mosselii MFY161 and P. aeruginosa PAO1. 4 h after infection of Caco-2/TC7 cells with the bacteria, extracellular germs were killed by gentamicin. Cells were lysed and the intracellular bacteria were enumerated by plating onto nutrient agar medium. Results were calculated as the mean values (+/−SEM) of three independent experiments. * P < 0.05 versus P. mosselii ATCC BAA-99 and P. mosselii MFY161, NS not significant between P. mosselii ATCC BAA-99 and P. mosselii MFY161.

Article Snippet: P. mosselii ATCC BAA-99 is a clinical strain isolated from tracheal aspirate of a patient suffering from pulmonary infections [ ].

Techniques: Infection

Journal: BMC Microbiology

Article Title: Cytotoxicity and inflammatory potential of two Pseudomonas mosselii strains isolated from clinical samples of hospitalized patients

doi: 10.1186/1471-2180-13-123

Figure Lengend Snippet: Proinflammatory effects of P. mosselii ATCC BAA-99, P. mosselii MFY161 and P. aeruginosa PAO1 on Caco-2/TC7 cells. IL-6 and IL-8 cytokines, and HBD-2 were measured in Caco-2/TC7 cells supernatant after 24 h of infection. Results were calculated as the mean values (+/−SEM) of three independent experiments. *** P < 0.001 versus uninfected Caco-2/TC7 cells, ** P < 0.01 versus uninfected Caco-2/TC7 cells, NS not significant.

Article Snippet: P. mosselii ATCC BAA-99 is a clinical strain isolated from tracheal aspirate of a patient suffering from pulmonary infections [ ].

Techniques: Infection

Journal: BMC Microbiology

Article Title: Cytotoxicity and inflammatory potential of two Pseudomonas mosselii strains isolated from clinical samples of hospitalized patients

doi: 10.1186/1471-2180-13-123

Figure Lengend Snippet: Effects of P. mosselii ATCC BAA-99, P. mosselii MFY161 and P. aeruginosa PAO1 on the transepithelial electrical resistance of Caco-2/TC7 cells. Differentiated Caco-2/TC7 cells were infected for 24 h. The TER was expressed as percentages of the initial control TER measured across each individual cell monolayer at the onset of the experiment. Results are the mean values (+/−SEM) of three independent experiments. *** P < 0.001 versus uninfected Caco-2/TC7 cells, ** P < 0.01 versus uninfected Caco-2/TC7 cells.

Article Snippet: P. mosselii ATCC BAA-99 is a clinical strain isolated from tracheal aspirate of a patient suffering from pulmonary infections [ ].

Techniques: Infection

Journal: BMC Microbiology

Article Title: Cytotoxicity and inflammatory potential of two Pseudomonas mosselii strains isolated from clinical samples of hospitalized patients

doi: 10.1186/1471-2180-13-123

Figure Lengend Snippet: Effects of P. mosselii ATCC BAA-99, P. mosselii MFY161 and P. aeruginosa PAO1 on the F-actin cytoskeleton of Caco-2/TC7 cells. Differentiated Caco-2/TC7 cells were infected for 24 h. F-actin was stained and examined using a confocal laser scanning microscope. ( A ) Uninfected cells, ( B ) infection by P. mosselii ATCC BAA-99, ( C ) infection by P. mosselii MFY161, ( D ) infection by P . aeruginosa PAO1. Scale bar 100 μm.

Article Snippet: P. mosselii ATCC BAA-99 is a clinical strain isolated from tracheal aspirate of a patient suffering from pulmonary infections [ ].

Techniques: Infection, Staining, Laser-Scanning Microscopy

Journal: BMC Microbiology

Article Title: Cytotoxicity and inflammatory potential of two Pseudomonas mosselii strains isolated from clinical samples of hospitalized patients

doi: 10.1186/1471-2180-13-123

Figure Lengend Snippet: Cytotoxic effects of P. mosselii ATCC BAA-99, P. mosselii MFY161 and P. aeruginosa PAO1 on Caco-2/TC7 cells. Cytotoxicity was determined by LDH release assay after 24 h of infection. Results were calculated as the mean values (+/−SEM) of three independent experiments. *** P < 0.001 versus uninfected Caco-2/TC7 cells, ∆∆∆ P < 0.001 versus P. aeruginosa PAO1 , ∆∆ P < 0.01 versus P. aeruginosa PAO1, •• P < 0.01 versus P. mosselii ATCC BAA-99.

Article Snippet: The bacterial proinflammatory effect of P. mosselii ATCC BAA-99 and MFY161 was then assessed by measuring the secretion of IL-6 and IL-8 cytokines in Caco-2/TC7 after 24 h of infection.

Techniques: Lactate Dehydrogenase Assay, Infection

Journal: BMC Microbiology

Article Title: Cytotoxicity and inflammatory potential of two Pseudomonas mosselii strains isolated from clinical samples of hospitalized patients

doi: 10.1186/1471-2180-13-123

Figure Lengend Snippet: Invasive capacity of P. mosselii ATCC BAA-99, P. mosselii MFY161 and P. aeruginosa PAO1. 4 h after infection of Caco-2/TC7 cells with the bacteria, extracellular germs were killed by gentamicin. Cells were lysed and the intracellular bacteria were enumerated by plating onto nutrient agar medium. Results were calculated as the mean values (+/−SEM) of three independent experiments. * P < 0.05 versus P. mosselii ATCC BAA-99 and P. mosselii MFY161, NS not significant between P. mosselii ATCC BAA-99 and P. mosselii MFY161.

Article Snippet: The bacterial proinflammatory effect of P. mosselii ATCC BAA-99 and MFY161 was then assessed by measuring the secretion of IL-6 and IL-8 cytokines in Caco-2/TC7 after 24 h of infection.

Techniques: Infection

Journal: BMC Microbiology

Article Title: Cytotoxicity and inflammatory potential of two Pseudomonas mosselii strains isolated from clinical samples of hospitalized patients

doi: 10.1186/1471-2180-13-123

Figure Lengend Snippet: Proinflammatory effects of P. mosselii ATCC BAA-99, P. mosselii MFY161 and P. aeruginosa PAO1 on Caco-2/TC7 cells. IL-6 and IL-8 cytokines, and HBD-2 were measured in Caco-2/TC7 cells supernatant after 24 h of infection. Results were calculated as the mean values (+/−SEM) of three independent experiments. *** P < 0.001 versus uninfected Caco-2/TC7 cells, ** P < 0.01 versus uninfected Caco-2/TC7 cells, NS not significant.

Article Snippet: The bacterial proinflammatory effect of P. mosselii ATCC BAA-99 and MFY161 was then assessed by measuring the secretion of IL-6 and IL-8 cytokines in Caco-2/TC7 after 24 h of infection.

Techniques: Infection

Journal: BMC Microbiology

Article Title: Cytotoxicity and inflammatory potential of two Pseudomonas mosselii strains isolated from clinical samples of hospitalized patients

doi: 10.1186/1471-2180-13-123

Figure Lengend Snippet: Effects of P. mosselii ATCC BAA-99, P. mosselii MFY161 and P. aeruginosa PAO1 on the transepithelial electrical resistance of Caco-2/TC7 cells. Differentiated Caco-2/TC7 cells were infected for 24 h. The TER was expressed as percentages of the initial control TER measured across each individual cell monolayer at the onset of the experiment. Results are the mean values (+/−SEM) of three independent experiments. *** P < 0.001 versus uninfected Caco-2/TC7 cells, ** P < 0.01 versus uninfected Caco-2/TC7 cells.

Article Snippet: The bacterial proinflammatory effect of P. mosselii ATCC BAA-99 and MFY161 was then assessed by measuring the secretion of IL-6 and IL-8 cytokines in Caco-2/TC7 after 24 h of infection.

Techniques: Infection

Journal: BMC Microbiology

Article Title: Cytotoxicity and inflammatory potential of two Pseudomonas mosselii strains isolated from clinical samples of hospitalized patients

doi: 10.1186/1471-2180-13-123

Figure Lengend Snippet: Effects of P. mosselii ATCC BAA-99, P. mosselii MFY161 and P. aeruginosa PAO1 on the F-actin cytoskeleton of Caco-2/TC7 cells. Differentiated Caco-2/TC7 cells were infected for 24 h. F-actin was stained and examined using a confocal laser scanning microscope. ( A ) Uninfected cells, ( B ) infection by P. mosselii ATCC BAA-99, ( C ) infection by P. mosselii MFY161, ( D ) infection by P . aeruginosa PAO1. Scale bar 100 μm.

Article Snippet: The bacterial proinflammatory effect of P. mosselii ATCC BAA-99 and MFY161 was then assessed by measuring the secretion of IL-6 and IL-8 cytokines in Caco-2/TC7 after 24 h of infection.

Techniques: Infection, Staining, Laser-Scanning Microscopy