p fluorescens migula strain pf 5  (ATCC)


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    Structured Review

    ATCC p fluorescens migula strain pf 5
    P Fluorescens Migula Strain Pf 5, supplied by ATCC, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p fluorescens migula strain pf 5/product/ATCC
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p fluorescens migula strain pf 5 - by Bioz Stars, 2020-02
    79/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Evolution of New Enzymatic Function by Structural Modulation of Cysteine Reactivity in Pseudomonas fluorescens Isocyanide Hydratase *
    Article Snippet: Paragraph title: Cloning and Mutagenesis of P. fluorescens pf-5 ICH ... The coding sequence for ICH was PCR-amplified from the genomic DNA of P. fluorescens Migula strain Pf-5 (American Type Culture Collection number BAA-477D) using primers that incorporated a 5′ NdeI restriction site and a 3′ XhoI restriction site.

    Mutagenesis:

    Article Title: Evolution of New Enzymatic Function by Structural Modulation of Cysteine Reactivity in Pseudomonas fluorescens Isocyanide Hydratase *
    Article Snippet: Paragraph title: Cloning and Mutagenesis of P. fluorescens pf-5 ICH ... The coding sequence for ICH was PCR-amplified from the genomic DNA of P. fluorescens Migula strain Pf-5 (American Type Culture Collection number BAA-477D) using primers that incorporated a 5′ NdeI restriction site and a 3′ XhoI restriction site.

    Polymerase Chain Reaction:

    Article Title: Evolution of New Enzymatic Function by Structural Modulation of Cysteine Reactivity in Pseudomonas fluorescens Isocyanide Hydratase *
    Article Snippet: .. The coding sequence for ICH was PCR-amplified from the genomic DNA of P. fluorescens Migula strain Pf-5 (American Type Culture Collection number BAA-477D) using primers that incorporated a 5′ NdeI restriction site and a 3′ XhoI restriction site. .. The ICH gene was cloned between the NdeI and XhoI restriction sites of the bacterial expression vector pET15b (Novagen, Darmstadt, Germany) such that the expressed protein carries an N-terminal, thrombin-cleavable hexahistidine tag.

    Expressing:

    Article Title: Evolution of New Enzymatic Function by Structural Modulation of Cysteine Reactivity in Pseudomonas fluorescens Isocyanide Hydratase *
    Article Snippet: The coding sequence for ICH was PCR-amplified from the genomic DNA of P. fluorescens Migula strain Pf-5 (American Type Culture Collection number BAA-477D) using primers that incorporated a 5′ NdeI restriction site and a 3′ XhoI restriction site. .. The ICH gene was cloned between the NdeI and XhoI restriction sites of the bacterial expression vector pET15b (Novagen, Darmstadt, Germany) such that the expressed protein carries an N-terminal, thrombin-cleavable hexahistidine tag.

    Sequencing:

    Article Title: Evolution of New Enzymatic Function by Structural Modulation of Cysteine Reactivity in Pseudomonas fluorescens Isocyanide Hydratase *
    Article Snippet: .. The coding sequence for ICH was PCR-amplified from the genomic DNA of P. fluorescens Migula strain Pf-5 (American Type Culture Collection number BAA-477D) using primers that incorporated a 5′ NdeI restriction site and a 3′ XhoI restriction site. .. The ICH gene was cloned between the NdeI and XhoI restriction sites of the bacterial expression vector pET15b (Novagen, Darmstadt, Germany) such that the expressed protein carries an N-terminal, thrombin-cleavable hexahistidine tag.

    Recombinant:

    Article Title: Evolution of New Enzymatic Function by Structural Modulation of Cysteine Reactivity in Pseudomonas fluorescens Isocyanide Hydratase *
    Article Snippet: The coding sequence for ICH was PCR-amplified from the genomic DNA of P. fluorescens Migula strain Pf-5 (American Type Culture Collection number BAA-477D) using primers that incorporated a 5′ NdeI restriction site and a 3′ XhoI restriction site. .. After thrombin cleavage, the final recombinant ICH protein has three vector-derived amino acids at the N terminus (GSH), resulting in a 231-amino acid protein with a calculated molecular mass of 24,158 Da.

    Plasmid Preparation:

    Article Title: Evolution of New Enzymatic Function by Structural Modulation of Cysteine Reactivity in Pseudomonas fluorescens Isocyanide Hydratase *
    Article Snippet: The coding sequence for ICH was PCR-amplified from the genomic DNA of P. fluorescens Migula strain Pf-5 (American Type Culture Collection number BAA-477D) using primers that incorporated a 5′ NdeI restriction site and a 3′ XhoI restriction site. .. The ICH gene was cloned between the NdeI and XhoI restriction sites of the bacterial expression vector pET15b (Novagen, Darmstadt, Germany) such that the expressed protein carries an N-terminal, thrombin-cleavable hexahistidine tag.

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  • 79
    ATCC p fluorescens migula strain pf 5
    P Fluorescens Migula Strain Pf 5, supplied by ATCC, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p fluorescens migula strain pf 5/product/ATCC
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p fluorescens migula strain pf 5 - by Bioz Stars, 2020-02
    79/100 stars
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