Journal: Neural Regeneration Research

Article Title: Prolonged intermittent theta burst stimulation restores the balance between A 2A R- and A 1 R-mediated adenosine signaling in the 6-hydroxidopamine model of Parkinson’s disease

doi: 10.4103/NRR.NRR-D-23-01542

Figure Lengend Snippet: Primary and secondary antibodies used in this study

Article Snippet: p-AMPK , Rabbit, polyclonal , 1:2000 WB , Cell Signaling Technology , 2535 , AB_331250.

Techniques: Western Blot, Immunohistochemistry

Journal: Neural Regeneration Research

Article Title: Prolonged intermittent theta burst stimulation restores the balance between A 2A R- and A 1 R-mediated adenosine signaling in the 6-hydroxidopamine model of Parkinson’s disease

doi: 10.4103/NRR.NRR-D-23-01542

Figure Lengend Snippet: Effects of iTBS on cytosolic pools of adenosine-generating and adenosine-eliminating enzymes and AMP kinase in crude synaptosomal fraction of the caudoputamen of 6-OHDA-lesioned rats. (A) Representative support membranes showing density of 55-kDa (eN/CD73) and 37-kDa (GAPDH) bands and histograms of immunoblot analysis showing eN/CD73 protein abundance in cytosolic fraction of the CPu of sham-stimulated and iTBS-stimulated animals at 1 and 3 weeks after stimulation. (B) Representative support membranes showing density of 42-kDa (ADA2) and 37-kDa (GAPDH) bands and histograms of immunoblot analysis showing ADA1 protein abundance in cytosolic fraction of the CPu of sham-stimulated and iTBS-stimulated animals 1 and 3 weeks after stimulation. (C) Representative support membranes showing density of 60-kDa (phospho-AMPK) and 60-kDa (total-AMPK) bands and histograms of immunoblot analysis showing ratio of p-AMPK and t-AMPK protein abundance in cytosolic fraction fractions of the CPu of sham-stimulated and iTBS-stimulated animals at 1 and 3 weeks after stimulation. All data are represented as mean ± SD. Dots in the graphs represent individual values. The number at the bottom of the graphs represents the number of individual animals included in analysis. All data are expressed as the percentage (%) of contralateral (left) CPu (dotted line at 100%). (D) The AMP phosphohydrolase/cN-II activity was assayed in the cytosolic fraction of the CPu from both sham-stimulated and iTBS-stimulated animals, at both 1 and 3 weeks after the stimulation. The bars depicted represent the mean activity (expressed as nmol Pi/mg/min) ± SEM, based on five determinations performed in duplicate. (E) The AMP phosphohydrolase activity was measured in both the absence and presence of the specific eN/CD73 inhibitor MRS 4592. The bars represent the mean activity (expressed as nmol Pi/mg/min) ± SEM, calculated from five determinations performed in duplicate. The numbers at the bottom of the graphs indicate the total number of individual animals included in the analysis. Significance shown inside graphs: * P < 0.05, *** P < 0.001, **** P < 0.0001 (unpaired Student’s t -test). All experiments are repeated at least twice. 6-OHDA: 6-Hydroxydopamine; CPu: caudoputamen; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; iTBS: intermittent theta burst stimulation; ns: not significant.

Article Snippet: p-AMPK , Rabbit, polyclonal , 1:2000 WB , Cell Signaling Technology , 2535 , AB_331250.

Techniques: Western Blot, Activity Assay

Journal: Molecular Biology Reports

Article Title: Single-cell sequencing reveals that AK5 inhibits apoptosis in AD oligodendrocytes by regulating the AMPK signaling pathway

doi: 10.1007/s11033-025-10311-x

Figure Lengend Snippet: Cellular assay showing AK5 inhibition of oligodendrocyte apoptosis through AMPK signalling activation. A , B mRNA ( A ) and protein ( B ) expression levels of AK5 in Aβ1-42-induced MO3.13 cells compared to control; C CCK8 assay showing changes in the survival rate of Aβ1-42-induced cells due to Ak5 overexpression and knockdown; D, E Apoptosis assay examining effects of AK5 overexpression and knockdown on apoptosis of Aβ1-42-induced cells; F Effects of AK5 overexpression and knockdown on protein expression abundance of P-AMPK, SITR1, P53, NF-KB in the AMPK pathway, and BAX, BCL-2, CASPASE3 in the apoptotic pathway

Article Snippet: The membranes were then incubated with primary antibodies at 4 ℃ overnight (ak5 (1:1000, Wuhan Sanying, China, 12,510–2-AP), p-AMPK (1:1000, Affinity Bioscience, USA, AF3423), SIRT1 (1:1000, Affinity Bioscience, USA, DF6033), p- NF-KB (1:1000, Affinity Bioscience, USA, AF2006), P53 (1:1000, Affinity Bioscience, USA, AF0879), Bcl-2 (1:1000, Affinity Bioscience, USA, AF6139), Bax (1:1000, Affinity Bioscience, USA, AF0120), Cleaved Caspase-3 (1:1000, Affinity Bioscience, USA, AF7022), GAPDH (1:5000, proteintech, USA, 60,004–1-Ig)).

Techniques: Inhibition, Activation Assay, Expressing, Control, CCK-8 Assay, Over Expression, Knockdown, Apoptosis Assay