Journal: Frontiers in Toxicology

Article Title: Optimisation of an in vitro human cardiovascular model on-a-chip for toxicological assessment of nicotine delivery products

doi: 10.3389/ftox.2024.1395670

Figure Lengend Snippet: (A) . Image and representative diagram of the 2-lane OrganoPlate. Boxes 1, 2 and 4 mark where reagents/cells can be added to the respective parts of the chip. x and y -axes show the chip orientation on the plate; (B) . Rocker with plate and diagrammatic representation of the direction and gradient of media flow; (C) . Diagrammatic representation of the formation of the cell layer following seeding of the human coronary artery endothelial cells (HCAECs) (cells shown in white) within the 2-lane OrganoPlate. Red areas represent the perfusion channel, blue areas the extracellular matrix (ECM) and grey the plate PhaseGuide. Bidirectional arrows represent the direction of flow and x and y -axes the orientation; g = gravity. (D) . Diagrammatic representation of the cell culture within the OrganoPlate 2-lane. The arrow represents the bidirectional flow of medium/suspension cells; green circles represent THP-1 cells; the endothelial cell vessel is represented in red and the extracellular collagen matrix in blue; (E) . Image of the 3D HCAEC vessel stained with CD31.

Article Snippet: The OrganoPlate 2-lane 96 (MIMETAS, 9605400B, Oegstgeest, Netherlands), consisting of 96 microfluidic chips with, respectively, 9 mm and 12.2 mm long gel and perfusion channels (400 µm × 220 µm (w x h)), was used in this study.

Techniques: Cell Culture, Suspension, Staining

Journal: Bioengineering

Article Title: Reproducibility and Robustness of a Liver Microphysiological System PhysioMimix LC12 under Varying Culture Conditions and Cell Type Combinations

doi: 10.3390/bioengineering10101195

Figure Lengend Snippet: General study designs for the experiments performed. The range of experimental conditions that was utilized in this study to evaluate PhysioMimix LC-12 and other liver in vitro models. See detailed experimental protocols for each experiment detailed in . Day 0 corresponded to the day when the cells were seeded into devices and media movement commenced (for microfluidic models) or cells were seeded into multi-well plates (for static cultures). In the PhysioMimix LC-12, media flow through the device/scaffolds without cells was initiated before cells were seeded, according to the manufacturer’s protocols. Media changes with or without CYP3A4 assay and the timing of drug/chemical additions are indicated (see symbols legend in the inset). ( A ) The most typical PhysioMimix LC-12 study design spanning a total of 14 days in culture. In the experiments that used NPCs, they were started in culture 2 days prior to the seeding of all cells into the device and initiation of flow. The asterisks (*, **) denote that different types of NPCs were used for PHHs and iHeps 2.0 (see ). ( B ) The PhysioMimix LC-12 study extended to 28 days. ( C ) Studies with iHeps 2.0 in the PhysioMimix LC-12 model. ( D ) Static culture in 384-well plates for iHeps 2.0. ( E ) Static cultures in 96-well plates for PHHs (HU8373 donor only). ( F ) Monoculture PHH (HU8373) study in a LAMPS model. ( G ) Study with iHeps 2.0 in the OrganoPlate 2-lane 96 (Mimetas, Leiden, The Netherlands) model. ( H ) Study of Trovafloxacin +/− LPS in PhysioMimix LC-12 model. ( I ) Study of the metabolism of a pesticide mixture in PhysioMimix LC-12 model.

Article Snippet: The OrganoPlate ® 2-lane 96 plate contains 96 devices, each of which contains one gel channel and one perfusion channel [ ] to enable the culture of a perfused tubule adjacent to the extracellular matrix without a membrane.

Techniques: In Vitro

Journal: Bioengineering

Article Title: Reproducibility and Robustness of a Liver Microphysiological System PhysioMimix LC12 under Varying Culture Conditions and Cell Type Combinations

doi: 10.3390/bioengineering10101195

Figure Lengend Snippet: Comparison of basal function across different cell sources and models. ( A ) Albumin and urea secretion, CYP3A4 activity, and LDH leakage in the PhysioMimix LC12 under monoculture conditions across 5 different PHH donors and iPSC-derived hepatocytes (iHeps 2.0). ( B ) Albumin and urea secretion, CYP3A4 activity, and LDH leakage in 2D culture or other liver MPS models (LAMPS or OrganoPlate ® 2-lane 96) under monoculture conditions using either PHH donor HU8373 or iHeps. ( C ) Albumin secretion and CYP3A4 activity in the PhysioMimix LC12 under monoculture and co-culture conditions using PHH donor HU8373 over 28 days of culture. The number of experiments included in each condition of use with a range of individual chips in each experiment is indicated in the labels.

Article Snippet: The OrganoPlate ® 2-lane 96 plate contains 96 devices, each of which contains one gel channel and one perfusion channel [ ] to enable the culture of a perfused tubule adjacent to the extracellular matrix without a membrane.

Techniques: Comparison, Activity Assay, Derivative Assay, Co-Culture Assay

Journal: Bioengineering

Article Title: Reproducibility and Robustness of a Liver Microphysiological System PhysioMimix LC12 under Varying Culture Conditions and Cell Type Combinations

doi: 10.3390/bioengineering10101195

Figure Lengend Snippet: Metabolism of a mixture of 20 pesticides across different cell sources and models. Metabolism is shown as % parent compound remaining after 48 h in the PhysioMimix LC-12 using PHH (donor HU8373) + THP-1 with either 1 µM (green squares) or 5 µM (green diamonds) equimolar mixture and compared to the published results in of experiments with iPSC-derived hepatocyte (iHeps 2.0) suspension, 2D culture, and OrganoPlate ® 2-lane 96 (abbreviated as “Mimetas”) experiments. Data shown are mean ± standard deviation (n = 3 in each condition).

Article Snippet: The OrganoPlate ® 2-lane 96 plate contains 96 devices, each of which contains one gel channel and one perfusion channel [ ] to enable the culture of a perfused tubule adjacent to the extracellular matrix without a membrane.

Techniques: Pesticides, Derivative Assay, Suspension, Standard Deviation