oregon green488 maleimide  (Thermo Fisher)


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    Name:
    Oregon Green 488 Maleimide
    Description:
    The thiol reactive Oregon Green 488 maleimide can be used to can be used to create green fluorescent bioconjugates with excitation emission maxima 496 524 nm This fluorinated analog of fluorescein overcomes some of the key limitations of fluorescein including greater photostability and a lower pKa pKa 4 7 versus 6 4 for fluorescein making its fluorescence essentially pH insensitive in the physiological pH range
    Catalog Number:
    o6034
    Price:
    None
    Applications:
    Cell Analysis|Labeling Proteins & Antibodies with Fluorophores|Labeling Thiols|Protein Biology|Protein Labeling|Protein Labeling & Crosslinking|Protein and Antibody Labeling|Protein, Peptide & Antibody Labeling|Thiol-Reactive Fluorophores|Labeling Chemistry
    Category:
    Labeling Detection Products
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    Structured Review

    Thermo Fisher oregon green488 maleimide
    The thiol reactive Oregon Green 488 maleimide can be used to can be used to create green fluorescent bioconjugates with excitation emission maxima 496 524 nm This fluorinated analog of fluorescein overcomes some of the key limitations of fluorescein including greater photostability and a lower pKa pKa 4 7 versus 6 4 for fluorescein making its fluorescence essentially pH insensitive in the physiological pH range
    https://www.bioz.com/result/oregon green488 maleimide/product/Thermo Fisher
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    oregon green488 maleimide - by Bioz Stars, 2020-08
    94/100 stars

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    Related Articles

    Transfection:

    Article Title: Anti-matrix metalloproteinase-9 DNAzyme decreases tumor growth in the MMTV-PyMT mouse model of breast cancer
    Article Snippet: .. The cells were then serum-starved for 4 hours prior to transient transfection with Oregon Green™488-maleimide-labeled AM9D or control DNAzyme (24 μg) using Lipofectamine 2000 (Invitrogen). ..

    Fluorescence:

    Article Title: Protein Interactions between Fe65, the LDL receptor-related protein and the amyloid precursor protein
    Article Snippet: .. To further establish the interaction between LRP-CT and Fe65, a truncated LRP-CT starting at position 4488 and ending at 4454 (LRP4488) was attached through an N-terminal cysteine to Oregon Green 488 maleimide (Invitrogen) and used in fluorescence anisotropy binding studies. .. LRP4488 or pYLRP4488 was incubated with varying concentrations of Fe65 WW-PID1-PID2 (236-662) or WW-PID1 (236-512) and the fluorescence anisotropy change upon binding was measured ( ).

    Mutagenesis:

    Article Title: Crystal structure of the PRC1 ubiquitylation module bound to the nucleosome
    Article Snippet: .. Nucleosome binding assays Nucleosome binding assays were performed with nucleosomes reconstituted with a H2B Ser112Cys mutant labeled with Oregon Green 488 maleimide (Life Technologies) with approximately ~50% efficiency and 147- or 185-bp 601 DNA. .. Fluorescence quenching assays were performed essentially as previously described .

    Labeling:

    Article Title: Processive ATP-driven Substrate Disassembly by the N-Ethylmaleimide-sensitive Factor (NSF) Molecular Machine *
    Article Snippet: .. SNAREs were co-expressed using the Duet expression system (Novagen), purified, and labeled with the self-quenching dye Oregon Green 488 maleimide (Invitrogen). .. The purity of the purified SNARE complex is high ( C , left two lanes ).

    Article Title: Crystal structure of the PRC1 ubiquitylation module bound to the nucleosome
    Article Snippet: .. Nucleosome binding assays Nucleosome binding assays were performed with nucleosomes reconstituted with a H2B Ser112Cys mutant labeled with Oregon Green 488 maleimide (Life Technologies) with approximately ~50% efficiency and 147- or 185-bp 601 DNA. .. Fluorescence quenching assays were performed essentially as previously described .

    Article Title: Protein Interactions between Fe65, the LDL receptor-related protein and the amyloid precursor protein
    Article Snippet: .. LRP4488 or LRP4488 phosphorylated at Y4507 (pYLRP4488) was labeled with Oregon Green 488 maleimide (invitrogen, O-6034) at the single cysteine introduced at the N-terminus according to the manufacturers instructions. .. 1 mL of 100 μM LRP4488 or pYLRP4488 was reduced with immobilized TCEP (Pierce) for 1 hour in TBS, pH 7.5 at room temperature.

    Purification:

    Article Title: Processive ATP-driven Substrate Disassembly by the N-Ethylmaleimide-sensitive Factor (NSF) Molecular Machine *
    Article Snippet: .. SNAREs were co-expressed using the Duet expression system (Novagen), purified, and labeled with the self-quenching dye Oregon Green 488 maleimide (Invitrogen). .. The purity of the purified SNARE complex is high ( C , left two lanes ).

    Expressing:

    Article Title: Processive ATP-driven Substrate Disassembly by the N-Ethylmaleimide-sensitive Factor (NSF) Molecular Machine *
    Article Snippet: .. SNAREs were co-expressed using the Duet expression system (Novagen), purified, and labeled with the self-quenching dye Oregon Green 488 maleimide (Invitrogen). .. The purity of the purified SNARE complex is high ( C , left two lanes ).

    Binding Assay:

    Article Title: Protein Interactions between Fe65, the LDL receptor-related protein and the amyloid precursor protein
    Article Snippet: .. To further establish the interaction between LRP-CT and Fe65, a truncated LRP-CT starting at position 4488 and ending at 4454 (LRP4488) was attached through an N-terminal cysteine to Oregon Green 488 maleimide (Invitrogen) and used in fluorescence anisotropy binding studies. .. LRP4488 or pYLRP4488 was incubated with varying concentrations of Fe65 WW-PID1-PID2 (236-662) or WW-PID1 (236-512) and the fluorescence anisotropy change upon binding was measured ( ).

    Article Title: Crystal structure of the PRC1 ubiquitylation module bound to the nucleosome
    Article Snippet: .. Nucleosome binding assays Nucleosome binding assays were performed with nucleosomes reconstituted with a H2B Ser112Cys mutant labeled with Oregon Green 488 maleimide (Life Technologies) with approximately ~50% efficiency and 147- or 185-bp 601 DNA. .. Fluorescence quenching assays were performed essentially as previously described .

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  • 94
    Thermo Fisher oregon green488 maleimide
    Oregon Green488 Maleimide, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oregon green488 maleimide/product/Thermo Fisher
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    oregon green488 maleimide - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

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