orbitrap q exactive hf  (Thermo Fisher)


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    Structured Review

    Thermo Fisher orbitrap q exactive hf
    Orbitrap Q Exactive Hf, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/orbitrap q exactive hf/product/Thermo Fisher
    Average 90 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    orbitrap q exactive hf - by Bioz Stars, 2020-09
    90/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Cryptic indole hydroxylation by a non-canonical terpenoid cyclase parallels bacterial xenobiotic detoxification
    Article Snippet: .. The extract was filtered and analysed by means of HPLC-HRMS (Exactive Plus Orbitrap Mass Spectrometer, Thermo Scientific) operating with a 150 × 2.1 mm 3 μ Betasil C18 column (Thermo Scientific) and a gradient from 5 to 98% solvent B in 15 min (solvent A, 0.3% (v/v ) HCOOH in water; solvent B, 0.1% (v/v ) HCOOH in acetonitrile) at a flow rate of 200 μl min−1 and UV/HRMS detection. ..

    Article Title: Endogenous ocular lipids as potential modulators of intraocular pressure, et al. Endogenous ocular lipids as potential modulators of intraocular pressure
    Article Snippet: .. For reversed‐phase chromatography, an HPLC Thermo Scientific Accela 600 LC System instrument was used to elute the lipids onto Q‐Exactive Orbitrap mass spectrometer, which is a hybrid quadrupole‐Orbitrap mass spectrometer (Thermo Fisher Scientific), with a resolving power up to 140 000 m/z 200, and internal mass accuracy of < 1 ppm RMS and external < 5 ppm RMS. ..

    Hydrophilic Interaction Liquid Chromatography:

    Article Title: Hepatic fat is a stronger correlate of key clinical and molecular abnormalities than visceral and abdominal subcutaneous fat in youth
    Article Snippet: .. Separate hydrophilic interaction liquid chromatography (HILIC) and C18 chromatography columns were coupled with detection by a high-resolution mass spectrometer (Q Exactive HF Orbitrap, Thermo Scientific, San Jose, California) operated in full scan mode at 120 000 resolution and mass to charge ratio (m/z) range 85–1275. .. During HILIC chromatography, the electrospray ionization (ESI) source was operated in positive ion mode.

    Flow Cytometry:

    Article Title: Cryptic indole hydroxylation by a non-canonical terpenoid cyclase parallels bacterial xenobiotic detoxification
    Article Snippet: .. The extract was filtered and analysed by means of HPLC-HRMS (Exactive Plus Orbitrap Mass Spectrometer, Thermo Scientific) operating with a 150 × 2.1 mm 3 μ Betasil C18 column (Thermo Scientific) and a gradient from 5 to 98% solvent B in 15 min (solvent A, 0.3% (v/v ) HCOOH in water; solvent B, 0.1% (v/v ) HCOOH in acetonitrile) at a flow rate of 200 μl min−1 and UV/HRMS detection. ..

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: AZA-MS: a novel multiparameter mass spectrometry method to determine the intracellular dynamics of azacitidine therapy in vivo
    Article Snippet: .. Mass spectrometry and data analysis LC–MS analysis was performed utilising an ultra-high-performance liquid chromatography system (Dionex u3000 system, Thermo Fisher Scientific) interfaced to an Orbitrap mass spectrometer (Q Exactive Plus, Thermo Fisher Scientific) using a heated electrospray interface operated in the positive ion mode. .. The chromatographic separation was performed on a 100 mm × 2.1 mm i.d., 3 μM , C30 column (Acclaim, Thermo Fisher Scientific) kept at 40 ºC.

    Chromatography:

    Article Title: Hepatic fat is a stronger correlate of key clinical and molecular abnormalities than visceral and abdominal subcutaneous fat in youth
    Article Snippet: .. Separate hydrophilic interaction liquid chromatography (HILIC) and C18 chromatography columns were coupled with detection by a high-resolution mass spectrometer (Q Exactive HF Orbitrap, Thermo Scientific, San Jose, California) operated in full scan mode at 120 000 resolution and mass to charge ratio (m/z) range 85–1275. .. During HILIC chromatography, the electrospray ionization (ESI) source was operated in positive ion mode.

    Mass Spectrometry:

    Article Title: AZA-MS: a novel multiparameter mass spectrometry method to determine the intracellular dynamics of azacitidine therapy in vivo
    Article Snippet: .. Mass spectrometry and data analysis LC–MS analysis was performed utilising an ultra-high-performance liquid chromatography system (Dionex u3000 system, Thermo Fisher Scientific) interfaced to an Orbitrap mass spectrometer (Q Exactive Plus, Thermo Fisher Scientific) using a heated electrospray interface operated in the positive ion mode. .. The chromatographic separation was performed on a 100 mm × 2.1 mm i.d., 3 μM , C30 column (Acclaim, Thermo Fisher Scientific) kept at 40 ºC.

    Article Title: Cryptic indole hydroxylation by a non-canonical terpenoid cyclase parallels bacterial xenobiotic detoxification
    Article Snippet: .. The extract was filtered and analysed by means of HPLC-HRMS (Exactive Plus Orbitrap Mass Spectrometer, Thermo Scientific) operating with a 150 × 2.1 mm 3 μ Betasil C18 column (Thermo Scientific) and a gradient from 5 to 98% solvent B in 15 min (solvent A, 0.3% (v/v ) HCOOH in water; solvent B, 0.1% (v/v ) HCOOH in acetonitrile) at a flow rate of 200 μl min−1 and UV/HRMS detection. ..

    Article Title: Purification and Characterization of Latent Polyphenol Oxidase from Apricot (Prunus armeniaca L.)
    Article Snippet: .. Tryptic digestions of Pa PPO were analyzed by nanoUHPLC–ESI–MS/MS using a high-resolution Orbitrap mass spectrometer (Dionex Ultimate 3000 RSLCnano, Q Exactive Orbitrap, Thermo Scientific). .. The data analysis was performed with Proteome Discoverer 1.4 by searching against the P. armeniaca entries from the UniProt database (containing all listed proteins from P. mume , P. persica , and P. armeniaca ).

    Article Title: The emerging role of native mass spectrometry in characterizing the structure and dynamics of macromolecular complexes
    Article Snippet: .. In 2012, Heck et al . first demonstrated the use of an Orbitrap mass spectrometer (Exactive Plus™, Thermo Fisher Scientific) for the analysis of intact protein complexes ranging from 150 kDa to 800 kDa. .. – They subsequently extended this range to include virus-like nanoparticles with a mass up to 4.5 MDa ( ).

    Article Title: Endogenous ocular lipids as potential modulators of intraocular pressure, et al. Endogenous ocular lipids as potential modulators of intraocular pressure
    Article Snippet: .. For reversed‐phase chromatography, an HPLC Thermo Scientific Accela 600 LC System instrument was used to elute the lipids onto Q‐Exactive Orbitrap mass spectrometer, which is a hybrid quadrupole‐Orbitrap mass spectrometer (Thermo Fisher Scientific), with a resolving power up to 140 000 m/z 200, and internal mass accuracy of < 1 ppm RMS and external < 5 ppm RMS. ..

    Article Title: Application of high-resolution mass spectrometry to measure low abundance isotope enrichment in individual muscle proteins
    Article Snippet: .. Analysis of [ring-13 C6 ]-phenylalanine enrichment was performed with a high-resolution orbitrap mass spectrometer (Thermo Q Exactive Plus) using a 14 min targeted MS/MS method. .. The orbitrap MS was operated at 70,000 resolution, with an AGC target of 5e6 and maximum injection time of 100 ms. An isolation width of 1.0 m/z was applied for isolation of the m+3 and m+6 precursor species at m/z 225.15 and 228.17, respectively, and MS/MS was performed in the HCD cell at 40 V. All resulting fragments within the m/z 50-250 window were detected in the orbitrap.

    Article Title: Label-free and isobaric tandem mass tag (TMT) multiplexed quantitative proteomic data of two contrasting rice cultivars exposed to drought stress and recovery
    Article Snippet: .. These fractions were desalted using C18 OMIX® tips (Agilent) and analysed on a Q Exactive Orbitrap mass spectrometer (Thermo Scientific) coupled to an EASY-nLC1000 (Thermo Scientific). ..

    Tandem Mass Spectroscopy:

    Article Title: Application of high-resolution mass spectrometry to measure low abundance isotope enrichment in individual muscle proteins
    Article Snippet: .. Analysis of [ring-13 C6 ]-phenylalanine enrichment was performed with a high-resolution orbitrap mass spectrometer (Thermo Q Exactive Plus) using a 14 min targeted MS/MS method. .. The orbitrap MS was operated at 70,000 resolution, with an AGC target of 5e6 and maximum injection time of 100 ms. An isolation width of 1.0 m/z was applied for isolation of the m+3 and m+6 precursor species at m/z 225.15 and 228.17, respectively, and MS/MS was performed in the HCD cell at 40 V. All resulting fragments within the m/z 50-250 window were detected in the orbitrap.

    Reversed-phase Chromatography:

    Article Title: Endogenous ocular lipids as potential modulators of intraocular pressure, et al. Endogenous ocular lipids as potential modulators of intraocular pressure
    Article Snippet: .. For reversed‐phase chromatography, an HPLC Thermo Scientific Accela 600 LC System instrument was used to elute the lipids onto Q‐Exactive Orbitrap mass spectrometer, which is a hybrid quadrupole‐Orbitrap mass spectrometer (Thermo Fisher Scientific), with a resolving power up to 140 000 m/z 200, and internal mass accuracy of < 1 ppm RMS and external < 5 ppm RMS. ..

    Liquid Chromatography:

    Article Title: AZA-MS: a novel multiparameter mass spectrometry method to determine the intracellular dynamics of azacitidine therapy in vivo
    Article Snippet: .. Mass spectrometry and data analysis LC–MS analysis was performed utilising an ultra-high-performance liquid chromatography system (Dionex u3000 system, Thermo Fisher Scientific) interfaced to an Orbitrap mass spectrometer (Q Exactive Plus, Thermo Fisher Scientific) using a heated electrospray interface operated in the positive ion mode. .. The chromatographic separation was performed on a 100 mm × 2.1 mm i.d., 3 μM , C30 column (Acclaim, Thermo Fisher Scientific) kept at 40 ºC.

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    Thermo Fisher q exactiveã cents ⠄ â cents hf hybrid quadrupole orbitrap
    Q Exactiveã Cents ⠄ â Cents Hf Hybrid Quadrupole Orbitrap, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q exactiveã cents ⠄ â cents hf hybrid quadrupole orbitrap/product/Thermo Fisher
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    q exactiveã cents ⠄ â cents hf hybrid quadrupole orbitrap - by Bioz Stars, 2020-09
    84/100 stars
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