optiquant hcv rna  (Thermo Fisher)


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    Structured Review

    Thermo Fisher optiquant hcv rna
    qRT-PCR of IFN-γ in chimpanzees infected with HEV or <t>HCV.</t> IFN-γ was tested by primer- and probe-specific TaqMan qPCR (see Table S1 in the supplemental material). Total <t>RNA</t> from liver biopsies was converted to cDNA, amplified, and tested
    Optiquant Hcv Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/optiquant hcv rna/product/Thermo Fisher
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    optiquant hcv rna - by Bioz Stars, 2020-05
    91/100 stars

    Related Products / Commonly Used Together

    real-time taqman assay

    Images

    1) Product Images from "Pathogenesis of Hepatitis E Virus and Hepatitis C Virus in Chimpanzees: Similarities and Differences ▿Pathogenesis of Hepatitis E Virus and Hepatitis C Virus in Chimpanzees: Similarities and Differences ▿ †"

    Article Title: Pathogenesis of Hepatitis E Virus and Hepatitis C Virus in Chimpanzees: Similarities and Differences ▿Pathogenesis of Hepatitis E Virus and Hepatitis C Virus in Chimpanzees: Similarities and Differences ▿ †

    Journal: Journal of Virology

    doi: 10.1128/JVI.01205-10

    qRT-PCR of IFN-γ in chimpanzees infected with HEV or HCV. IFN-γ was tested by primer- and probe-specific TaqMan qPCR (see Table S1 in the supplemental material). Total RNA from liver biopsies was converted to cDNA, amplified, and tested
    Figure Legend Snippet: qRT-PCR of IFN-γ in chimpanzees infected with HEV or HCV. IFN-γ was tested by primer- and probe-specific TaqMan qPCR (see Table S1 in the supplemental material). Total RNA from liver biopsies was converted to cDNA, amplified, and tested

    Techniques Used: Quantitative RT-PCR, Infection, Real-time Polymerase Chain Reaction, Amplification

    2) Product Images from "Infection with hepatitis C virus depends on TACSTD2, a regulator of claudin-1 and occludin highly downregulated in hepatocellular carcinoma"

    Article Title: Infection with hepatitis C virus depends on TACSTD2, a regulator of claudin-1 and occludin highly downregulated in hepatocellular carcinoma

    Journal: PLoS Pathogens

    doi: 10.1371/journal.ppat.1006916

    Gene expression profiling and HCV RNA in tumor and nontumorous liver tissue of patients with HCV-associated HCC. (A) Liver samples were obtained from five different liver areas: the center of the tumor (A, 8 samples), the periphery of the tumor (B, 29 samples), the perilesional nontumorous area outside the tumor margin (C, 21 samples), the area at a distance of 2–3 cm from the margin of the tumor (D, 22 samples), and the most distant nontumorous area, on the edge of the liver (E, 23 samples). Data from the center of the tumor area (A) represent individual samples, whereas data from each of the other liver areas (B to E) represent the average of multiple specimens obtained in the 4 directions. Differentially expressed genes (n = 1,115) were identified by comparing A (tumor) and E (non-tumor) areas (FDR
    Figure Legend Snippet: Gene expression profiling and HCV RNA in tumor and nontumorous liver tissue of patients with HCV-associated HCC. (A) Liver samples were obtained from five different liver areas: the center of the tumor (A, 8 samples), the periphery of the tumor (B, 29 samples), the perilesional nontumorous area outside the tumor margin (C, 21 samples), the area at a distance of 2–3 cm from the margin of the tumor (D, 22 samples), and the most distant nontumorous area, on the edge of the liver (E, 23 samples). Data from the center of the tumor area (A) represent individual samples, whereas data from each of the other liver areas (B to E) represent the average of multiple specimens obtained in the 4 directions. Differentially expressed genes (n = 1,115) were identified by comparing A (tumor) and E (non-tumor) areas (FDR

    Techniques Used: Expressing

    3) Product Images from "Infection with hepatitis C virus depends on TACSTD2, a regulator of claudin-1 and occludin highly downregulated in hepatocellular carcinoma"

    Article Title: Infection with hepatitis C virus depends on TACSTD2, a regulator of claudin-1 and occludin highly downregulated in hepatocellular carcinoma

    Journal: PLoS Pathogens

    doi: 10.1371/journal.ppat.1006916

    Gene expression profiling and HCV RNA in tumor and nontumorous liver tissue of patients with HCV-associated HCC. ]. (C) Heat map representing the most upregulated transmembrane genes in hepatoma cell lines: Huh7.5 and HepG2. (D) Quantitative RT-PCR of the 4 HCV-entry cofactors CLDN1, CD81, OCLN, and SR-B1 in tumor, nontumorous tissue, Huh7.5 cells, and primary human hepatocytes (PHH). Data represent the log2-transformed ratio of each co-receptor to GAPDH. No differences were found between tumor and non-tumor in any of the 4 HCV-entry cofactors. Comparable levels were also observed for PHH except for a lower expression of CLDN1, whereas Huh7.5 showed a higher expression of 3 entry cofactors (CD81, OCLN and SR-B1). (E) Visualization of CLDN1 (green surface rendering) and OCLN (red surface rendering) in tumor and non-tumor specimens in a representative patient who exhibited a 3 log drop in HCV RNA levels within the tumor by immunofluorescence staining. Two patterns of CLDN1 and OCLN cellular distribution were documented: the tumor shows a clumpy distribution of CLDN1 and OCLN whereas the matching nontumorous tissue shows a regular linear distribution along the cellular membrane. DAPI is represented in blue. Overlay images show all channels in 3D volume rendering. The H E images show the tumor and nontumorous liver specimens of the same patient (40X). (F) Quantitative RT-PCR of TACSTD2. Mean relative quantities are normalized to the non-tumor tissue, expressed as 2 -ΔΔCT , where ΔΔC T is the average difference between the target tissue ΔC T and the non-tumor tissue ΔC T .
    Figure Legend Snippet: Gene expression profiling and HCV RNA in tumor and nontumorous liver tissue of patients with HCV-associated HCC. ]. (C) Heat map representing the most upregulated transmembrane genes in hepatoma cell lines: Huh7.5 and HepG2. (D) Quantitative RT-PCR of the 4 HCV-entry cofactors CLDN1, CD81, OCLN, and SR-B1 in tumor, nontumorous tissue, Huh7.5 cells, and primary human hepatocytes (PHH). Data represent the log2-transformed ratio of each co-receptor to GAPDH. No differences were found between tumor and non-tumor in any of the 4 HCV-entry cofactors. Comparable levels were also observed for PHH except for a lower expression of CLDN1, whereas Huh7.5 showed a higher expression of 3 entry cofactors (CD81, OCLN and SR-B1). (E) Visualization of CLDN1 (green surface rendering) and OCLN (red surface rendering) in tumor and non-tumor specimens in a representative patient who exhibited a 3 log drop in HCV RNA levels within the tumor by immunofluorescence staining. Two patterns of CLDN1 and OCLN cellular distribution were documented: the tumor shows a clumpy distribution of CLDN1 and OCLN whereas the matching nontumorous tissue shows a regular linear distribution along the cellular membrane. DAPI is represented in blue. Overlay images show all channels in 3D volume rendering. The H E images show the tumor and nontumorous liver specimens of the same patient (40X). (F) Quantitative RT-PCR of TACSTD2. Mean relative quantities are normalized to the non-tumor tissue, expressed as 2 -ΔΔCT , where ΔΔC T is the average difference between the target tissue ΔC T and the non-tumor tissue ΔC T .

    Techniques Used: Expressing, Quantitative RT-PCR, Transformation Assay, Immunofluorescence, Staining

    4) Product Images from "Development of a TaqMan Assay for the Six Major Genotypes of Hepatitis C Virus: Comparison With Commercial Assays"

    Article Title: Development of a TaqMan Assay for the Six Major Genotypes of Hepatitis C Virus: Comparison With Commercial Assays

    Journal:

    doi: 10.1002/jmv.21043

    Titration of a prototype HCV H77 strain using three NAT assays for quantifying HCV RNA. The HCV H strain was tested by TaqMan (open circles), Versant HCV RNA 3.0 b-DNA (diamonds) and the HCV Monitor 2.0 (solid circles). Based on linear regression and
    Figure Legend Snippet: Titration of a prototype HCV H77 strain using three NAT assays for quantifying HCV RNA. The HCV H strain was tested by TaqMan (open circles), Versant HCV RNA 3.0 b-DNA (diamonds) and the HCV Monitor 2.0 (solid circles). Based on linear regression and

    Techniques Used: Titration

    Comparison of three NAT assays for quantifying HCV RNA. Weekly samples from chimpanzees experimentally infected with HCV genotypes 1a (TN), 1b (Con1 strain), or 6a (HK strain) were tested with the Versant HCV RNA 3.0 b-DNA assay (solid diamonds), HCV
    Figure Legend Snippet: Comparison of three NAT assays for quantifying HCV RNA. Weekly samples from chimpanzees experimentally infected with HCV genotypes 1a (TN), 1b (Con1 strain), or 6a (HK strain) were tested with the Versant HCV RNA 3.0 b-DNA assay (solid diamonds), HCV

    Techniques Used: Infection

    Related Articles

    Construct:

    Article Title: Pathogenesis of Hepatitis E Virus and Hepatitis C Virus in Chimpanzees: Similarities and Differences ▿Pathogenesis of Hepatitis E Virus and Hepatitis C Virus in Chimpanzees: Similarities and Differences ▿ †
    Article Snippet: .. A quantity standard line representing a 6-log range was constructed with the OptiQuant HCV RNA (Acrometrix, Benicia, CA) nucleic acid test reference panel. ..

    Article Title: Infection with hepatitis C virus depends on TACSTD2, a regulator of claudin-1 and occludin highly downregulated in hepatocellular carcinoma
    Article Snippet: .. A standard curve comprising a 6-log dynamic range was constructed with the OptiQuant HCV RNA (Acrometrix, Benicia, CA) nucleic acid reference panel. ..

    TaqMan Assay:

    Article Title: Development of a TaqMan Assay for the Six Major Genotypes of Hepatitis C Virus: Comparison With Commercial Assays
    Article Snippet: .. In order to generate a standard line for our real-time TaqMan assay, we used the 5 × 106 IU/ml panel member of the OptiQuant HCV RNA (Acrometrix, Benicia, CA) NAT reference panel. ..