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Sigma-Genosys open reading frame orfs
Open Reading Frame Orfs, supplied by Sigma-Genosys, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/open reading frame orfs/product/Sigma-Genosys
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
open reading frame orfs - by Bioz Stars, 2020-09
85/100 stars

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Amplification:

Article Title: Characterization of Genetically Matched Isolates of Campylobacter jejuni Reveals that Mutations in Genes Involved in Flagellar Biosynthesis Alter the Organism's Virulence Potential ▿
Article Snippet: .. DNA fragments of individual open reading frames (ORFs) were amplified using primers specific for ORFs present in strain NCTC 11168 (Sigma Genosys, The Woodlands, TX). .. Each PCR (total reaction volume, 100 μl) consisted of 1× MasterAmp Taq PCR buffer, 1× MasterAmp Taq enhancer, 2.5 mM MgCl2 , 200 μM each dNTP, forward and reverse primers at 0.2 μM each, 0.5 U of MasterAmp Taq DNA polymerase (Epicenter, Madison, WI), and approximately 50 ng of genomic DNA from strain NCTC 11168.

Article Title: Comparative Genomic Analysis of Clinical Strains of Campylobacter jejuni from South Africa
Article Snippet: .. Construction of the C. jejuni DNA microarray DNA fragments of individual open reading frames (ORFs) were amplified by using the Sigma-Genosys (The Woodlands, TX) C. jejuni ORFmer primer set specific for strain NTCT11168 coding sequences and by using primers from Operon Technologies (Alameda, CA) specific for unique sequences in strain RM1221, as previously described . .. Additional DNA fragments were amplified from strain RM3193 ( ) based on the published sequence for the serotype HS∶41 capsular locus ORFs and for LOS genes from locus classes A, B, C, E and F, as in previous studies .

Article Title: AI-2 does not function as a quorum sensing molecule in Campylobacter jejuni during exponential growth in vitro
Article Snippet: .. Construction of the C. jejuni DNA microarray Internal DNA fragments corresponding to unique segments of the individual open reading frames (ORFs) in the annotated genome sequence of strain NCTC 11168 [ ] were amplified by PCR using gene-specific primers (Sigma Genosys ORFmer set), then purified and spotted on GAPSII slides (Corning, USA) using an in-house Stanford designed microarrayer as previously described [ ]. .. Transcriptome analysis Labelled cDNA was prepared from 15 μg RNA using Stratascript RT (Stratagene, UK) with the direct incorporation of Cy3 and Cy5 dyes (Amersham, UK), applied to microarrays, washed, scanned and statistically analysed as described by Holmes et al .

Article Title: Culture of Campylobacter jejuni with Sodium Deoxycholate Induces Virulence Gene Expression
Article Snippet: .. DNA fragments of individual open reading frames (ORFs) were amplified with the Sigma-Genosys (The Woodlands, TX) C. jejuni ORFmer primer set specific for strain NCTC 11168 coding sequences and with primers from Operon Technologies (Alameda, CA) specific for strain RM1221 unique sequences, as described previously ( ). .. The PCR products were purified with a Qiagen 8000 robot by using a QIAquick 96-well Biorobot kit (Qiagen, Valencia, CA).

Polymerase Chain Reaction:

Article Title: Comparative Genomics of Canadian Epidemic Lineages of Methicillin-Resistant Staphylococcus aureus ▿ ▿ †
Article Snippet: .. S. aureus ORFmer PCR primer pairs, representing 2,741 open reading frames (ORFs) from S. aureus Col, were purchased from Sigma Genosys (St. Louis, MO). .. PCR was performed using the manufacturer's guidelines and confirmed using agarose gel electrophoresis.

Article Title: AI-2 does not function as a quorum sensing molecule in Campylobacter jejuni during exponential growth in vitro
Article Snippet: .. Construction of the C. jejuni DNA microarray Internal DNA fragments corresponding to unique segments of the individual open reading frames (ORFs) in the annotated genome sequence of strain NCTC 11168 [ ] were amplified by PCR using gene-specific primers (Sigma Genosys ORFmer set), then purified and spotted on GAPSII slides (Corning, USA) using an in-house Stanford designed microarrayer as previously described [ ]. .. Transcriptome analysis Labelled cDNA was prepared from 15 μg RNA using Stratascript RT (Stratagene, UK) with the direct incorporation of Cy3 and Cy5 dyes (Amersham, UK), applied to microarrays, washed, scanned and statistically analysed as described by Holmes et al .

Labeling:

Article Title: Engineering a Homo-Ethanol Pathway in Escherichia coli: Increased Glycolytic Flux and Levels of Expression of Glycolytic Genes during Xylose Fermentation †
Article Snippet: .. A set of primers homologous to the 3′ ends of the predicted 4,290 open reading frame (ORFs) in E. coli was obtained from Sigma-Genosys (Panorama E. coli cDNA labeling primers). .. These primers were used to prepare cDNA based on a modification of the protocol provided by the manufacturer.

Purification:

Article Title: AI-2 does not function as a quorum sensing molecule in Campylobacter jejuni during exponential growth in vitro
Article Snippet: .. Construction of the C. jejuni DNA microarray Internal DNA fragments corresponding to unique segments of the individual open reading frames (ORFs) in the annotated genome sequence of strain NCTC 11168 [ ] were amplified by PCR using gene-specific primers (Sigma Genosys ORFmer set), then purified and spotted on GAPSII slides (Corning, USA) using an in-house Stanford designed microarrayer as previously described [ ]. .. Transcriptome analysis Labelled cDNA was prepared from 15 μg RNA using Stratascript RT (Stratagene, UK) with the direct incorporation of Cy3 and Cy5 dyes (Amersham, UK), applied to microarrays, washed, scanned and statistically analysed as described by Holmes et al .

Microarray:

Article Title: Comparative Genomic Analysis of Clinical Strains of Campylobacter jejuni from South Africa
Article Snippet: .. Construction of the C. jejuni DNA microarray DNA fragments of individual open reading frames (ORFs) were amplified by using the Sigma-Genosys (The Woodlands, TX) C. jejuni ORFmer primer set specific for strain NTCT11168 coding sequences and by using primers from Operon Technologies (Alameda, CA) specific for unique sequences in strain RM1221, as previously described . .. Additional DNA fragments were amplified from strain RM3193 ( ) based on the published sequence for the serotype HS∶41 capsular locus ORFs and for LOS genes from locus classes A, B, C, E and F, as in previous studies .

Article Title: AI-2 does not function as a quorum sensing molecule in Campylobacter jejuni during exponential growth in vitro
Article Snippet: .. Construction of the C. jejuni DNA microarray Internal DNA fragments corresponding to unique segments of the individual open reading frames (ORFs) in the annotated genome sequence of strain NCTC 11168 [ ] were amplified by PCR using gene-specific primers (Sigma Genosys ORFmer set), then purified and spotted on GAPSII slides (Corning, USA) using an in-house Stanford designed microarrayer as previously described [ ]. .. Transcriptome analysis Labelled cDNA was prepared from 15 μg RNA using Stratascript RT (Stratagene, UK) with the direct incorporation of Cy3 and Cy5 dyes (Amersham, UK), applied to microarrays, washed, scanned and statistically analysed as described by Holmes et al .

Sequencing:

Article Title: AI-2 does not function as a quorum sensing molecule in Campylobacter jejuni during exponential growth in vitro
Article Snippet: .. Construction of the C. jejuni DNA microarray Internal DNA fragments corresponding to unique segments of the individual open reading frames (ORFs) in the annotated genome sequence of strain NCTC 11168 [ ] were amplified by PCR using gene-specific primers (Sigma Genosys ORFmer set), then purified and spotted on GAPSII slides (Corning, USA) using an in-house Stanford designed microarrayer as previously described [ ]. .. Transcriptome analysis Labelled cDNA was prepared from 15 μg RNA using Stratascript RT (Stratagene, UK) with the direct incorporation of Cy3 and Cy5 dyes (Amersham, UK), applied to microarrays, washed, scanned and statistically analysed as described by Holmes et al .

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    Sigma-Genosys open reading frames orfs
    Open Reading Frames Orfs, supplied by Sigma-Genosys, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/open reading frames orfs/product/Sigma-Genosys
    Average 91 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    open reading frames orfs - by Bioz Stars, 2020-09
    91/100 stars
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