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TaKaRa online in fusion hd cloning kit
Online In Fusion Hd Cloning Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/online in fusion hd cloning kit/product/TaKaRa
Average 80 stars, based on 1 article reviews
Price from $9.99 to $1999.99
online in fusion hd cloning kit - by Bioz Stars, 2020-04
80/100 stars

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Related Articles

Clone Assay:

Article Title: Equine Mx1 Restricts Influenza A Virus Replication by Targeting at Distinct Site of its Nucleoprotein
Article Snippet: .. Mutants of pcAGGS-H7N9-NP and pEF-JL89-NP were constructed according to the online In-Fusion® HD Cloning Kit (Clontech, Felicia, CA, USA) user manual ( http://www.clontech.com/CN/Products/Cloning_and_Competent_Cells/Clonin_Kits/xxclt_searchResults.jsp ). .. Briefly, the fragments of the pCAGGS/pcDNA3.1 vector and each target gene were amplified and were then fused using the In-Fusion® HD Enzyme kit (Clontech, Felicia, CA, USA).

Amplification:

Article Title: Equine Mx1 Restricts Influenza A Virus Replication by Targeting at Distinct Site of its Nucleoprotein
Article Snippet: Mutants of pcAGGS-H7N9-NP and pEF-JL89-NP were constructed according to the online In-Fusion® HD Cloning Kit (Clontech, Felicia, CA, USA) user manual ( http://www.clontech.com/CN/Products/Cloning_and_Competent_Cells/Clonin_Kits/xxclt_searchResults.jsp ). .. Briefly, the fragments of the pCAGGS/pcDNA3.1 vector and each target gene were amplified and were then fused using the In-Fusion® HD Enzyme kit (Clontech, Felicia, CA, USA).

Construct:

Article Title: Equine Mx1 Restricts Influenza A Virus Replication by Targeting at Distinct Site of its Nucleoprotein
Article Snippet: .. Mutants of pcAGGS-H7N9-NP and pEF-JL89-NP were constructed according to the online In-Fusion® HD Cloning Kit (Clontech, Felicia, CA, USA) user manual ( http://www.clontech.com/CN/Products/Cloning_and_Competent_Cells/Clonin_Kits/xxclt_searchResults.jsp ). .. Briefly, the fragments of the pCAGGS/pcDNA3.1 vector and each target gene were amplified and were then fused using the In-Fusion® HD Enzyme kit (Clontech, Felicia, CA, USA).

Polymerase Chain Reaction:

Article Title: Equine Mx1 Restricts Influenza A Virus Replication by Targeting at Distinct Site of its Nucleoprotein
Article Snippet: Site-directed mutants of NP sequences were generated using overlapping PCR and identified by DNA sequencing. .. Mutants of pcAGGS-H7N9-NP and pEF-JL89-NP were constructed according to the online In-Fusion® HD Cloning Kit (Clontech, Felicia, CA, USA) user manual ( http://www.clontech.com/CN/Products/Cloning_and_Competent_Cells/Clonin_Kits/xxclt_searchResults.jsp ).

Generated:

Article Title: Equine Mx1 Restricts Influenza A Virus Replication by Targeting at Distinct Site of its Nucleoprotein
Article Snippet: Site-directed mutants of NP sequences were generated using overlapping PCR and identified by DNA sequencing. .. Mutants of pcAGGS-H7N9-NP and pEF-JL89-NP were constructed according to the online In-Fusion® HD Cloning Kit (Clontech, Felicia, CA, USA) user manual ( http://www.clontech.com/CN/Products/Cloning_and_Competent_Cells/Clonin_Kits/xxclt_searchResults.jsp ).

DNA Sequencing:

Article Title: Equine Mx1 Restricts Influenza A Virus Replication by Targeting at Distinct Site of its Nucleoprotein
Article Snippet: Site-directed mutants of NP sequences were generated using overlapping PCR and identified by DNA sequencing. .. Mutants of pcAGGS-H7N9-NP and pEF-JL89-NP were constructed according to the online In-Fusion® HD Cloning Kit (Clontech, Felicia, CA, USA) user manual ( http://www.clontech.com/CN/Products/Cloning_and_Competent_Cells/Clonin_Kits/xxclt_searchResults.jsp ).

Sequencing:

Article Title: Equine Mx1 Restricts Influenza A Virus Replication by Targeting at Distinct Site of its Nucleoprotein
Article Snippet: Mutants of pcAGGS-H7N9-NP and pEF-JL89-NP were constructed according to the online In-Fusion® HD Cloning Kit (Clontech, Felicia, CA, USA) user manual ( http://www.clontech.com/CN/Products/Cloning_and_Competent_Cells/Clonin_Kits/xxclt_searchResults.jsp ). .. This sequence was then fused with the JL89-NP C-terminal (201–480) fragment, where pEF-JL89-NP was used to amplify the inserted C-terminal (201–480) fragments.

Plasmid Preparation:

Article Title: Equine Mx1 Restricts Influenza A Virus Replication by Targeting at Distinct Site of its Nucleoprotein
Article Snippet: Then, the fragments were successfully cloned in pcDNA3.1-HA vector (pcDNA3.1 (+) vector (Invitrogen, ThermoFisher, Waltham, MA, USA) that possessed 2X HA tags situated on the C-terminus and also p3X-Flag-CMV vector (Sigma Aldrich, St. Louis, MO, USA) that contained 3X Flag tags located at the N-terminus. .. Mutants of pcAGGS-H7N9-NP and pEF-JL89-NP were constructed according to the online In-Fusion® HD Cloning Kit (Clontech, Felicia, CA, USA) user manual ( http://www.clontech.com/CN/Products/Cloning_and_Competent_Cells/Clonin_Kits/xxclt_searchResults.jsp ).

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    TaKaRa online in fusion hd cloning kit
    Online In Fusion Hd Cloning Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/online in fusion hd cloning kit/product/TaKaRa
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    online in fusion hd cloning kit - by Bioz Stars, 2020-04
    80/100 stars
      Buy from Supplier

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