one taq hot start 2x pcr master mix  (New England Biolabs)


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    Name:
    Hot Start Taq 2X Master Mix
    Description:
    Hot Start Taq 2X Master Mix 500 rxns
    Catalog Number:
    m0496l
    Price:
    233
    Size:
    500 rxns
    Category:
    Thermostable DNA Polymerases
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    Structured Review

    New England Biolabs one taq hot start 2x pcr master mix
    Hot Start Taq 2X Master Mix
    Hot Start Taq 2X Master Mix 500 rxns
    https://www.bioz.com/result/one taq hot start 2x pcr master mix/product/New England Biolabs
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    one taq hot start 2x pcr master mix - by Bioz Stars, 2020-03
    99/100 stars

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    Related Articles

    Diagnostic Assay:

    Article Title: Rpn (YhgA-Like) Proteins of Escherichia coli K-12 and Their Contribution to RecA-Independent Horizontal Transfer
    Article Snippet: .. PCRs used to generate sequencing templates or genetic constructions were performed with Q5 high-fidelity DNA polymerase (catalog number M0491; NEB), while diagnostic PCRs used the Hot Start Taq 2× master mix (catalog number M0496; NEB). ..

    Clone Assay:

    Article Title: An epigenetic mechanism for cavefish eye degeneration
    Article Snippet: .. Portions of the coding regions of genes were PCR amplified using One Taq Hotstart 2X master mix in standard buffer DNA polymerase (NEB) and cloned into pCRII-TOPO TA vector (Thermofisher). .. Sequence verified clones were used to generate antisense riboprobes using appropriate enzymes.

    Amplification:

    Article Title: New insights into valve-related intramural and intracellular bacterial diversity in infective endocarditis
    Article Snippet: Paragraph title: DNA extraction and 16S rDNA amplification ... Briefly, each 20 μL of PCR reaction mixture contained 20 ng of total or microbial-enriched DNA as a template, 10 μL 2x Hot Start Taq Master Mix (New England BioLabs), and 0.5 μM of each primer.

    Article Title: Rapid regrowth and detection of microbial contaminants in equine fecal microbiome samples
    Article Snippet: Paragraph title: PCR amplification of the V4 region of the 16S rRNA gene and NGS ... PCR was performed in 25 μl vol and included: 2 μl (7.5 μM concn) of forward and reverse primers, 12.5 μl of Hot Start Taq 2X Master Mix (New England BioLabs Inc., Ipswich, MA., USA), 10 mM Tris-HCl, 2.5 μl of 10X bovine serum albumin (400 ng/μl final concn), and 2 μl of sample DNA.

    Article Title: An epigenetic mechanism for cavefish eye degeneration
    Article Snippet: .. Portions of the coding regions of genes were PCR amplified using One Taq Hotstart 2X master mix in standard buffer DNA polymerase (NEB) and cloned into pCRII-TOPO TA vector (Thermofisher). .. Sequence verified clones were used to generate antisense riboprobes using appropriate enzymes.

    Article Title: Fermented Animal Source Protein as Substitution of Fishmeal on Intestinal Microbiota, Immune-Related Cytokines and Resistance to Vibrio mimicus in Freshwater Crayfish (Cherax cainii)
    Article Snippet: PCR reactions were carried out in a total volume of 50 μL of master mixture, containing Hot Start Taq 2X Master Mix (New England BioLabs Inc., Ipswich, MA, United States) (25 μL), template DNA (2 μL), V3-V4 sequencing primers (1 μL each), and nuclease-free water (21 μL). .. Amplified PCR products were then separated by gel electrophoresis (Bio-Rad Laboratories Inc., Hercules, CA, United States) and visualized under gel doc (FujiFilm LAS–4000 Image Analyzer, Boston Inc., Foster City, CA, United States).

    Construct:

    Article Title: Demonstration of End-to-End Automation of DNA Data Storage
    Article Snippet: .. Sequencing preparation The extended adapter was constructed from a 1 kilobase fragment that was PCR-amplified from the lambda genome using hot start TAQ DNA polymerase (NEB M0496) with a Bsa-I restriction site added by the forward primer. ..

    Article Title: Rpn (YhgA-Like) Proteins of Escherichia coli K-12 and Their Contribution to RecA-Independent Horizontal Transfer
    Article Snippet: DNA constructs were created using an NEBuilder HiFi DNA assembly kit (catalog number E5520; NEB). .. PCRs used to generate sequencing templates or genetic constructions were performed with Q5 high-fidelity DNA polymerase (catalog number M0491; NEB), while diagnostic PCRs used the Hot Start Taq 2× master mix (catalog number M0496; NEB).

    Real-time Polymerase Chain Reaction:

    Article Title: The relationship between insecticide resistance, mosquito age and malaria prevalence in Anopheles gambiae s.l. from Guinea
    Article Snippet: At both LSHTM and CDC, PCR reactions were prepared using Hot Start Taq 2X Master Mix (New England Biolabs) with each reaction containing 12.5 µl of master mix, a final concentration of 1 µM of each primer, 2 µl template DNA, to a final reaction volume of 25 µl. .. Prepared reactions were run on a BIO-RAD QPCR system for 5 min at 95˚C, followed by 30 cycles of 95˚C for 30 sec, 52˚C for 30 sec and 72˚C for 1 min and a final extension of 72˚C for 5 min. PCR products were digested using the Alu I restriction enzyme through incubation at 37˚C for 16 hr followed by 65˚C for 20 min. DNA fragments were visualised on 2% Egel EX agarose gels (Invitrogen) with SYBR safe and an Invitrogen E-gel iBase Real-Time Transilluminator.

    Article Title: The relationship between insecticide resistance, mosquito age and malaria prevalence in Anopheles gambiae s.l. from Guinea
    Article Snippet: At both LSHTM and CDC, PCR reactions were prepared using Hot Start Taq 2X Master Mix (New England Biolabs) with each reaction containing 12.5 µl of master mix and variable final concentrations of primers (IPCF 0.1 µM, AltRev 0.1 µM, West WT 1 µM, West West 1.1 µM) for a final reaction volume of 25 µl. .. Prepared reactions were run on a BIO-RAD QPCR system for 5 min at 95˚C, followed by 35 cycles of 95˚C for 30 sec, 59˚C for 30 sec and 72˚C for 30 sec and a final extension of 72˚C for 5 min. PCR products were separated and visualised using 2% Egel EX agarose gels (Invitrogen) with SYBR safe and an Invitrogen E-gel iBase Real-Time Transilluminator.

    Incubation:

    Article Title: The relationship between insecticide resistance, mosquito age and malaria prevalence in Anopheles gambiae s.l. from Guinea
    Article Snippet: At both LSHTM and CDC, PCR reactions were prepared using Hot Start Taq 2X Master Mix (New England Biolabs) with each reaction containing 12.5 µl of master mix, a final concentration of 1 µM of each primer, 2 µl template DNA, to a final reaction volume of 25 µl. .. Prepared reactions were run on a BIO-RAD QPCR system for 5 min at 95˚C, followed by 30 cycles of 95˚C for 30 sec, 52˚C for 30 sec and 72˚C for 1 min and a final extension of 72˚C for 5 min. PCR products were digested using the Alu I restriction enzyme through incubation at 37˚C for 16 hr followed by 65˚C for 20 min. DNA fragments were visualised on 2% Egel EX agarose gels (Invitrogen) with SYBR safe and an Invitrogen E-gel iBase Real-Time Transilluminator.

    Expressing:

    Article Title: The Evolutionarily Conserved Cassette Exon 7b Drives ERG's Oncogenic Properties
    Article Snippet: Hot Start Taq 2× master mix (New England Biolabs) was used for standard PCR. .. The expression of ERG was measured using the primers listed in .

    Produced:

    Article Title: The relationship between insecticide resistance, mosquito age and malaria prevalence in Anopheles gambiae s.l. from Guinea
    Article Snippet: Sequences were assembled manually in BioEdit v7.0.9.0 sequence alignment editor software (Ibis Biosciences, USA) and unambiguous consensus sequences were produced for each individual. .. At both LSHTM and CDC, PCR reactions were prepared using Hot Start Taq 2X Master Mix (New England Biolabs) with each reaction containing 12.5 µl of master mix, a final concentration of 1 µM of each primer, 2 µl template DNA, to a final reaction volume of 25 µl.

    Ligation:

    Article Title: Sensitive and specific miRNA detection method using SplintR Ligase
    Article Snippet: To compare ligation activites, 10 pmol of enzyme was used in each ligation reaction according to the manufactures protocol. .. The One Taq Hot Start 2X PCR Master Mix was obtained from NEB.

    Generated:

    Article Title: An epigenetic mechanism for cavefish eye degeneration
    Article Snippet: Riboprobe synthesis, In situ hybridizations and histology Antisense riboprobes were generated using Roche DIG and FITC labeling mix. .. Portions of the coding regions of genes were PCR amplified using One Taq Hotstart 2X master mix in standard buffer DNA polymerase (NEB) and cloned into pCRII-TOPO TA vector (Thermofisher).

    Article Title: The relationship between insecticide resistance, mosquito age and malaria prevalence in Anopheles gambiae s.l. from Guinea
    Article Snippet: Sequencing reactions were purified using the Big Dye® XterminatorTM Purification Kit (Applied Biosystems, USA), according to the manufacturer’s protocol and data were generated using a 3500xL Genetic Analyzer (Applied Biosystems, USA). .. At both LSHTM and CDC, PCR reactions were prepared using Hot Start Taq 2X Master Mix (New England Biolabs) with each reaction containing 12.5 µl of master mix, a final concentration of 1 µM of each primer, 2 µl template DNA, to a final reaction volume of 25 µl.

    Polymerase Chain Reaction:

    Article Title: Sensitive and specific miRNA detection method using SplintR Ligase
    Article Snippet: .. The One Taq Hot Start 2X PCR Master Mix was obtained from NEB. .. DNA and RNA Escherichia coli rRNA was obtained from New England Biolabs.

    Article Title: New insights into valve-related intramural and intracellular bacterial diversity in infective endocarditis
    Article Snippet: .. Briefly, each 20 μL of PCR reaction mixture contained 20 ng of total or microbial-enriched DNA as a template, 10 μL 2x Hot Start Taq Master Mix (New England BioLabs), and 0.5 μM of each primer. .. PCR reactions were carried out using the following protocols: (1) for the V1-V3 hypervariable region, an initial denaturation step performed at 95°C for 30 s followed by 30 cycles of denaturation (95°C, 30 s), annealing (56°C, 40 s), and extension (68°C, 1 min), and a final elongation of 5 min at 68°C; (2) for the V3-V5 hypervariable region, the parameters were the same as above except that the annealing temperature was 59°C.

    Article Title: Rapid regrowth and detection of microbial contaminants in equine fecal microbiome samples
    Article Snippet: .. PCR was performed in 25 μl vol and included: 2 μl (7.5 μM concn) of forward and reverse primers, 12.5 μl of Hot Start Taq 2X Master Mix (New England BioLabs Inc., Ipswich, MA., USA), 10 mM Tris-HCl, 2.5 μl of 10X bovine serum albumin (400 ng/μl final concn), and 2 μl of sample DNA. .. Thermal cycle conditions were 95°C for 3 min for initial denaturing step, followed by 30 cycles of 95°C for 30 s, 50°C for 1 min, and 72°C for 1 min. PCR products were checked on a 2% agarose gel for correct product size formation (approx 350 bp) and quantified using GelAnalyzer software.

    Article Title: Demonstration of End-to-End Automation of DNA Data Storage
    Article Snippet: .. Sequencing preparation The extended adapter was constructed from a 1 kilobase fragment that was PCR-amplified from the lambda genome using hot start TAQ DNA polymerase (NEB M0496) with a Bsa-I restriction site added by the forward primer. ..

    Article Title: An epigenetic mechanism for cavefish eye degeneration
    Article Snippet: .. Portions of the coding regions of genes were PCR amplified using One Taq Hotstart 2X master mix in standard buffer DNA polymerase (NEB) and cloned into pCRII-TOPO TA vector (Thermofisher). .. Sequence verified clones were used to generate antisense riboprobes using appropriate enzymes.

    Article Title: Pyrimidine Biosynthesis Regulates the Small-Colony Variant and Mucoidy in Pseudomonas aeruginosa through Sigma Factor Competition
    Article Snippet: Paragraph title: PCR. ... All PCRs were run using the Hot Start Taq 2× master mix (New England Biolabs, Ipswich, MA).

    Article Title: Molecular evidence for the first records of facultative parthenogenesis in elapid snakes
    Article Snippet: .. PCR reactions to add the full-length Illumina adapters [ ] were performed in eight replicates per library pool in 30 µl volumes containing 10 µl of purified library, 1× Hot Start Taq Master Mix (NEB) and 0.66 µM each of the forward and reverse primers. ..

    Article Title: The Evolutionarily Conserved Cassette Exon 7b Drives ERG's Oncogenic Properties
    Article Snippet: .. Hot Start Taq 2× master mix (New England Biolabs) was used for standard PCR. .. The expression of ERG was measured using the primers listed in .

    Article Title: Fermented Animal Source Protein as Substitution of Fishmeal on Intestinal Microbiota, Immune-Related Cytokines and Resistance to Vibrio mimicus in Freshwater Crayfish (Cherax cainii)
    Article Snippet: .. PCR reactions were carried out in a total volume of 50 μL of master mixture, containing Hot Start Taq 2X Master Mix (New England BioLabs Inc., Ipswich, MA, United States) (25 μL), template DNA (2 μL), V3-V4 sequencing primers (1 μL each), and nuclease-free water (21 μL). .. A total of 30 cycles of reactions were performed in a BioRad S100 Gradient Thermal Cycler (Bio-Rad Laboratories, Inc., Foster City, CA, United States).

    Article Title: The relationship between insecticide resistance, mosquito age and malaria prevalence in Anopheles gambiae s.l. from Guinea
    Article Snippet: .. At both LSHTM and CDC, PCR reactions were prepared using Hot Start Taq 2X Master Mix (New England Biolabs) with each reaction containing 12.5 µl of master mix, a final concentration of 1 µM of each primer, 2 µl template DNA, to a final reaction volume of 25 µl. .. Prepared reactions were run on a BIO-RAD QPCR system for 5 min at 95˚C, followed by 30 cycles of 95˚C for 30 sec, 52˚C for 30 sec and 72˚C for 1 min and a final extension of 72˚C for 5 min. PCR products were digested using the Alu I restriction enzyme through incubation at 37˚C for 16 hr followed by 65˚C for 20 min. DNA fragments were visualised on 2% Egel EX agarose gels (Invitrogen) with SYBR safe and an Invitrogen E-gel iBase Real-Time Transilluminator.

    Article Title: Dietary supplementation of black soldier fly (Hermetica illucens) meal modulates gut microbiota, innate immune response and health status of marron (Cherax cainii, Austin 2002) fed poultry-by-product and fishmeal based diets
    Article Snippet: .. PCR master mixture was prepared as 50 µL final concentration containing 25 µL Hot Start 2X Master Mix (New England BioLabs Inc., Ipswich, MA, USA), two µL of template DNA, one µL of each forward and reverse V3–V4 sequencing primers, and 21 µL of nuclease-free water. .. The PCR was performed using BioRad S100 Gradient Thermal Cycler (Bio-Rad Laboratories, Inc., Foster City, CA, USA), under the following conditions: 2 min initial denaturation at 94 °C; 30 cycles of denaturation (30 s at 94 °C), annealing (1 min at 55 °C), and extension (1 min at 68 °C); a final extension at 68 °C for 5 min and holding the temperature at 10 °C.

    Article Title: Antibiotic resistance pattern and virulence genes content in avian pathogenic Escherichia coli (APEC) from broiler chickens in Chitwan, Nepal
    Article Snippet: .. The PCR was performed in 25 μL volume containing 12.5 μL Hot start Taq 2X master mix (BioLab Inc., New England), 1 μL each primer, 2 μL DNA template, and 8.5 μL nuclease free water. .. The PCR amplifications were conducted in MultiGene OptiMax Thermal Cycler (Labnet International, Inc., North America) and the cycling conditions were identical for all the samples as follows: 94 °C for 4 min; 35 cycles of 30 s at 94 °C, 1 min at 60 °C, and 2 min at 68 °C; and 72 °C for 7 min.

    Article Title: The relationship between insecticide resistance, mosquito age and malaria prevalence in Anopheles gambiae s.l. from Guinea
    Article Snippet: .. At both LSHTM and CDC, PCR reactions were prepared using Hot Start Taq 2X Master Mix (New England Biolabs) with each reaction containing 12.5 µl of master mix and variable final concentrations of primers (IPCF 0.1 µM, AltRev 0.1 µM, West WT 1 µM, West West 1.1 µM) for a final reaction volume of 25 µl. .. Prepared reactions were run on a BIO-RAD QPCR system for 5 min at 95˚C, followed by 35 cycles of 95˚C for 30 sec, 59˚C for 30 sec and 72˚C for 30 sec and a final extension of 72˚C for 5 min. PCR products were separated and visualised using 2% Egel EX agarose gels (Invitrogen) with SYBR safe and an Invitrogen E-gel iBase Real-Time Transilluminator.

    Multiplexing:

    Article Title: Rapid regrowth and detection of microbial contaminants in equine fecal microbiome samples
    Article Snippet: The fusion primers contained Ion Primer Adapter A on the 5’ end, followed by a unique 10 bp Hamming-error-correcting barcode [ ], a 3 bp ‘GAT’ spacer, and the 515F primer sequence to allow for multiplexing of samples. .. PCR was performed in 25 μl vol and included: 2 μl (7.5 μM concn) of forward and reverse primers, 12.5 μl of Hot Start Taq 2X Master Mix (New England BioLabs Inc., Ipswich, MA., USA), 10 mM Tris-HCl, 2.5 μl of 10X bovine serum albumin (400 ng/μl final concn), and 2 μl of sample DNA.

    Nucleic Acid Electrophoresis:

    Article Title: The Evolutionarily Conserved Cassette Exon 7b Drives ERG's Oncogenic Properties
    Article Snippet: Paragraph title: Semiquantitative Standard PCR and Gel Electrophoresis ... Hot Start Taq 2× master mix (New England Biolabs) was used for standard PCR.

    Article Title: Fermented Animal Source Protein as Substitution of Fishmeal on Intestinal Microbiota, Immune-Related Cytokines and Resistance to Vibrio mimicus in Freshwater Crayfish (Cherax cainii)
    Article Snippet: PCR reactions were carried out in a total volume of 50 μL of master mixture, containing Hot Start Taq 2X Master Mix (New England BioLabs Inc., Ipswich, MA, United States) (25 μL), template DNA (2 μL), V3-V4 sequencing primers (1 μL each), and nuclease-free water (21 μL). .. Amplified PCR products were then separated by gel electrophoresis (Bio-Rad Laboratories Inc., Hercules, CA, United States) and visualized under gel doc (FujiFilm LAS–4000 Image Analyzer, Boston Inc., Foster City, CA, United States).

    Article Title: Antibiotic resistance pattern and virulence genes content in avian pathogenic Escherichia coli (APEC) from broiler chickens in Chitwan, Nepal
    Article Snippet: The quality of genomic DNA was checked by gel electrophoresis and measuring absorbance at A 260 / A 280 and A 260 / A 230 ratios using the MaestroNano spectrophotometer (MaestroGen; Model name: MN-913). .. The PCR was performed in 25 μL volume containing 12.5 μL Hot start Taq 2X master mix (BioLab Inc., New England), 1 μL each primer, 2 μL DNA template, and 8.5 μL nuclease free water.

    Magnetic Beads:

    Article Title: Rapid regrowth and detection of microbial contaminants in equine fecal microbiome samples
    Article Snippet: PCR was performed in 25 μl vol and included: 2 μl (7.5 μM concn) of forward and reverse primers, 12.5 μl of Hot Start Taq 2X Master Mix (New England BioLabs Inc., Ipswich, MA., USA), 10 mM Tris-HCl, 2.5 μl of 10X bovine serum albumin (400 ng/μl final concn), and 2 μl of sample DNA. .. The product mixture was size selected by first using a 2% agarose gel selection, (E-gel Size-Select 2%, Life Technologies, Grand Island, NY, USA) followed by an additional purification step with AMPure XP DNA magnetic beads (Beckman Coulter, Danvers, MA) according to manufacturers protocol.

    Mutagenesis:

    Article Title: The relationship between insecticide resistance, mosquito age and malaria prevalence in Anopheles gambiae s.l. from Guinea
    Article Snippet: Paragraph title: Insecticide target site mutation detection ... At both LSHTM and CDC, PCR reactions were prepared using Hot Start Taq 2X Master Mix (New England Biolabs) with each reaction containing 12.5 µl of master mix, a final concentration of 1 µM of each primer, 2 µl template DNA, to a final reaction volume of 25 µl.

    Isolation:

    Article Title: Antibiotic resistance pattern and virulence genes content in avian pathogenic Escherichia coli (APEC) from broiler chickens in Chitwan, Nepal
    Article Snippet: Isolated E. coli strains were investigated for the presence of eleven virulence genes ( iutA, iss, papC, iucD, tsh, irp-2, ompT, hlyF, iron, cva/cvi, and astA ) which are associated with colibacillosis. .. The PCR was performed in 25 μL volume containing 12.5 μL Hot start Taq 2X master mix (BioLab Inc., New England), 1 μL each primer, 2 μL DNA template, and 8.5 μL nuclease free water.

    Size-exclusion Chromatography:

    Article Title: The relationship between insecticide resistance, mosquito age and malaria prevalence in Anopheles gambiae s.l. from Guinea
    Article Snippet: At both LSHTM and CDC, PCR reactions were prepared using Hot Start Taq 2X Master Mix (New England Biolabs) with each reaction containing 12.5 µl of master mix, a final concentration of 1 µM of each primer, 2 µl template DNA, to a final reaction volume of 25 µl. .. Prepared reactions were run on a BIO-RAD QPCR system for 5 min at 95˚C, followed by 30 cycles of 95˚C for 30 sec, 52˚C for 30 sec and 72˚C for 1 min and a final extension of 72˚C for 5 min. PCR products were digested using the Alu I restriction enzyme through incubation at 37˚C for 16 hr followed by 65˚C for 20 min. DNA fragments were visualised on 2% Egel EX agarose gels (Invitrogen) with SYBR safe and an Invitrogen E-gel iBase Real-Time Transilluminator.

    Article Title: The relationship between insecticide resistance, mosquito age and malaria prevalence in Anopheles gambiae s.l. from Guinea
    Article Snippet: At both LSHTM and CDC, PCR reactions were prepared using Hot Start Taq 2X Master Mix (New England Biolabs) with each reaction containing 12.5 µl of master mix and variable final concentrations of primers (IPCF 0.1 µM, AltRev 0.1 µM, West WT 1 µM, West West 1.1 µM) for a final reaction volume of 25 µl. .. Prepared reactions were run on a BIO-RAD QPCR system for 5 min at 95˚C, followed by 35 cycles of 95˚C for 30 sec, 59˚C for 30 sec and 72˚C for 30 sec and a final extension of 72˚C for 5 min. PCR products were separated and visualised using 2% Egel EX agarose gels (Invitrogen) with SYBR safe and an Invitrogen E-gel iBase Real-Time Transilluminator.

    Labeling:

    Article Title: An epigenetic mechanism for cavefish eye degeneration
    Article Snippet: Riboprobe synthesis, In situ hybridizations and histology Antisense riboprobes were generated using Roche DIG and FITC labeling mix. .. Portions of the coding regions of genes were PCR amplified using One Taq Hotstart 2X master mix in standard buffer DNA polymerase (NEB) and cloned into pCRII-TOPO TA vector (Thermofisher).

    Purification:

    Article Title: Rapid regrowth and detection of microbial contaminants in equine fecal microbiome samples
    Article Snippet: PCR was performed in 25 μl vol and included: 2 μl (7.5 μM concn) of forward and reverse primers, 12.5 μl of Hot Start Taq 2X Master Mix (New England BioLabs Inc., Ipswich, MA., USA), 10 mM Tris-HCl, 2.5 μl of 10X bovine serum albumin (400 ng/μl final concn), and 2 μl of sample DNA. .. The product mixture was size selected by first using a 2% agarose gel selection, (E-gel Size-Select 2%, Life Technologies, Grand Island, NY, USA) followed by an additional purification step with AMPure XP DNA magnetic beads (Beckman Coulter, Danvers, MA) according to manufacturers protocol.

    Article Title: Molecular evidence for the first records of facultative parthenogenesis in elapid snakes
    Article Snippet: .. PCR reactions to add the full-length Illumina adapters [ ] were performed in eight replicates per library pool in 30 µl volumes containing 10 µl of purified library, 1× Hot Start Taq Master Mix (NEB) and 0.66 µM each of the forward and reverse primers. ..

    Article Title: The relationship between insecticide resistance, mosquito age and malaria prevalence in Anopheles gambiae s.l. from Guinea
    Article Snippet: Sequencing reactions were purified using the Big Dye® XterminatorTM Purification Kit (Applied Biosystems, USA), according to the manufacturer’s protocol and data were generated using a 3500xL Genetic Analyzer (Applied Biosystems, USA). .. At both LSHTM and CDC, PCR reactions were prepared using Hot Start Taq 2X Master Mix (New England Biolabs) with each reaction containing 12.5 µl of master mix, a final concentration of 1 µM of each primer, 2 µl template DNA, to a final reaction volume of 25 µl.

    Sequencing:

    Article Title: Rapid regrowth and detection of microbial contaminants in equine fecal microbiome samples
    Article Snippet: The primers produce a V4 product insert sequence of approx 250 bp which gives adequate resolution for taxonomic analysis [ ]. .. PCR was performed in 25 μl vol and included: 2 μl (7.5 μM concn) of forward and reverse primers, 12.5 μl of Hot Start Taq 2X Master Mix (New England BioLabs Inc., Ipswich, MA., USA), 10 mM Tris-HCl, 2.5 μl of 10X bovine serum albumin (400 ng/μl final concn), and 2 μl of sample DNA.

    Article Title: Demonstration of End-to-End Automation of DNA Data Storage
    Article Snippet: .. Sequencing preparation The extended adapter was constructed from a 1 kilobase fragment that was PCR-amplified from the lambda genome using hot start TAQ DNA polymerase (NEB M0496) with a Bsa-I restriction site added by the forward primer. ..

    Article Title: An epigenetic mechanism for cavefish eye degeneration
    Article Snippet: Portions of the coding regions of genes were PCR amplified using One Taq Hotstart 2X master mix in standard buffer DNA polymerase (NEB) and cloned into pCRII-TOPO TA vector (Thermofisher). .. Sequence verified clones were used to generate antisense riboprobes using appropriate enzymes.

    Article Title: Rpn (YhgA-Like) Proteins of Escherichia coli K-12 and Their Contribution to RecA-Independent Horizontal Transfer
    Article Snippet: .. PCRs used to generate sequencing templates or genetic constructions were performed with Q5 high-fidelity DNA polymerase (catalog number M0491; NEB), while diagnostic PCRs used the Hot Start Taq 2× master mix (catalog number M0496; NEB). ..

    Article Title: Fermented Animal Source Protein as Substitution of Fishmeal on Intestinal Microbiota, Immune-Related Cytokines and Resistance to Vibrio mimicus in Freshwater Crayfish (Cherax cainii)
    Article Snippet: .. PCR reactions were carried out in a total volume of 50 μL of master mixture, containing Hot Start Taq 2X Master Mix (New England BioLabs Inc., Ipswich, MA, United States) (25 μL), template DNA (2 μL), V3-V4 sequencing primers (1 μL each), and nuclease-free water (21 μL). .. A total of 30 cycles of reactions were performed in a BioRad S100 Gradient Thermal Cycler (Bio-Rad Laboratories, Inc., Foster City, CA, United States).

    Article Title: The relationship between insecticide resistance, mosquito age and malaria prevalence in Anopheles gambiae s.l. from Guinea
    Article Snippet: Sequences were assembled manually in BioEdit v7.0.9.0 sequence alignment editor software (Ibis Biosciences, USA) and unambiguous consensus sequences were produced for each individual. .. At both LSHTM and CDC, PCR reactions were prepared using Hot Start Taq 2X Master Mix (New England Biolabs) with each reaction containing 12.5 µl of master mix, a final concentration of 1 µM of each primer, 2 µl template DNA, to a final reaction volume of 25 µl.

    Article Title: Dietary supplementation of black soldier fly (Hermetica illucens) meal modulates gut microbiota, innate immune response and health status of marron (Cherax cainii, Austin 2002) fed poultry-by-product and fishmeal based diets
    Article Snippet: .. PCR master mixture was prepared as 50 µL final concentration containing 25 µL Hot Start 2X Master Mix (New England BioLabs Inc., Ipswich, MA, USA), two µL of template DNA, one µL of each forward and reverse V3–V4 sequencing primers, and 21 µL of nuclease-free water. .. The PCR was performed using BioRad S100 Gradient Thermal Cycler (Bio-Rad Laboratories, Inc., Foster City, CA, USA), under the following conditions: 2 min initial denaturation at 94 °C; 30 cycles of denaturation (30 s at 94 °C), annealing (1 min at 55 °C), and extension (1 min at 68 °C); a final extension at 68 °C for 5 min and holding the temperature at 10 °C.

    Salting Out:

    Article Title: Molecular evidence for the first records of facultative parthenogenesis in elapid snakes
    Article Snippet: Genotyping and analyses Tissue samples from adults and offspring were collected, and DNA was extracted using a ‘salting-out’ method [ ] and the extracts quantified using the Quantus Fluorometer (Promega) as per the manufacturer's instructions. .. PCR reactions to add the full-length Illumina adapters [ ] were performed in eight replicates per library pool in 30 µl volumes containing 10 µl of purified library, 1× Hot Start Taq Master Mix (NEB) and 0.66 µM each of the forward and reverse primers.

    Plasmid Preparation:

    Article Title: An epigenetic mechanism for cavefish eye degeneration
    Article Snippet: .. Portions of the coding regions of genes were PCR amplified using One Taq Hotstart 2X master mix in standard buffer DNA polymerase (NEB) and cloned into pCRII-TOPO TA vector (Thermofisher). .. Sequence verified clones were used to generate antisense riboprobes using appropriate enzymes.

    Software:

    Article Title: Rapid regrowth and detection of microbial contaminants in equine fecal microbiome samples
    Article Snippet: PCR was performed in 25 μl vol and included: 2 μl (7.5 μM concn) of forward and reverse primers, 12.5 μl of Hot Start Taq 2X Master Mix (New England BioLabs Inc., Ipswich, MA., USA), 10 mM Tris-HCl, 2.5 μl of 10X bovine serum albumin (400 ng/μl final concn), and 2 μl of sample DNA. .. Thermal cycle conditions were 95°C for 3 min for initial denaturing step, followed by 30 cycles of 95°C for 30 s, 50°C for 1 min, and 72°C for 1 min. PCR products were checked on a 2% agarose gel for correct product size formation (approx 350 bp) and quantified using GelAnalyzer software.

    Article Title: The relationship between insecticide resistance, mosquito age and malaria prevalence in Anopheles gambiae s.l. from Guinea
    Article Snippet: Sequences were assembled manually in BioEdit v7.0.9.0 sequence alignment editor software (Ibis Biosciences, USA) and unambiguous consensus sequences were produced for each individual. .. At both LSHTM and CDC, PCR reactions were prepared using Hot Start Taq 2X Master Mix (New England Biolabs) with each reaction containing 12.5 µl of master mix, a final concentration of 1 µM of each primer, 2 µl template DNA, to a final reaction volume of 25 µl.

    Negative Control:

    Article Title: New insights into valve-related intramural and intracellular bacterial diversity in infective endocarditis
    Article Snippet: Briefly, each 20 μL of PCR reaction mixture contained 20 ng of total or microbial-enriched DNA as a template, 10 μL 2x Hot Start Taq Master Mix (New England BioLabs), and 0.5 μM of each primer. .. Water was used as negative control in the PCR reaction.

    Selection:

    Article Title: Rapid regrowth and detection of microbial contaminants in equine fecal microbiome samples
    Article Snippet: PCR was performed in 25 μl vol and included: 2 μl (7.5 μM concn) of forward and reverse primers, 12.5 μl of Hot Start Taq 2X Master Mix (New England BioLabs Inc., Ipswich, MA., USA), 10 mM Tris-HCl, 2.5 μl of 10X bovine serum albumin (400 ng/μl final concn), and 2 μl of sample DNA. .. The product mixture was size selected by first using a 2% agarose gel selection, (E-gel Size-Select 2%, Life Technologies, Grand Island, NY, USA) followed by an additional purification step with AMPure XP DNA magnetic beads (Beckman Coulter, Danvers, MA) according to manufacturers protocol.

    Agarose Gel Electrophoresis:

    Article Title: Rapid regrowth and detection of microbial contaminants in equine fecal microbiome samples
    Article Snippet: PCR was performed in 25 μl vol and included: 2 μl (7.5 μM concn) of forward and reverse primers, 12.5 μl of Hot Start Taq 2X Master Mix (New England BioLabs Inc., Ipswich, MA., USA), 10 mM Tris-HCl, 2.5 μl of 10X bovine serum albumin (400 ng/μl final concn), and 2 μl of sample DNA. .. Thermal cycle conditions were 95°C for 3 min for initial denaturing step, followed by 30 cycles of 95°C for 30 s, 50°C for 1 min, and 72°C for 1 min. PCR products were checked on a 2% agarose gel for correct product size formation (approx 350 bp) and quantified using GelAnalyzer software.

    Article Title: Antibiotic resistance pattern and virulence genes content in avian pathogenic Escherichia coli (APEC) from broiler chickens in Chitwan, Nepal
    Article Snippet: The PCR was performed in 25 μL volume containing 12.5 μL Hot start Taq 2X master mix (BioLab Inc., New England), 1 μL each primer, 2 μL DNA template, and 8.5 μL nuclease free water. .. The amplicons were analyzed by agarose gel electrophoresis with 1.5% agarose gel (Sigma-Aldrich, A4718) prepared in 1× TBE buffer (ThermoFisher Scientific, B52).

    In Situ:

    Article Title: An epigenetic mechanism for cavefish eye degeneration
    Article Snippet: Paragraph title: Riboprobe synthesis, In situ hybridizations and histology ... Portions of the coding regions of genes were PCR amplified using One Taq Hotstart 2X master mix in standard buffer DNA polymerase (NEB) and cloned into pCRII-TOPO TA vector (Thermofisher).

    Next-Generation Sequencing:

    Article Title: Rapid regrowth and detection of microbial contaminants in equine fecal microbiome samples
    Article Snippet: Paragraph title: PCR amplification of the V4 region of the 16S rRNA gene and NGS ... PCR was performed in 25 μl vol and included: 2 μl (7.5 μM concn) of forward and reverse primers, 12.5 μl of Hot Start Taq 2X Master Mix (New England BioLabs Inc., Ipswich, MA., USA), 10 mM Tris-HCl, 2.5 μl of 10X bovine serum albumin (400 ng/μl final concn), and 2 μl of sample DNA.

    Article Title: Dietary supplementation of black soldier fly (Hermetica illucens) meal modulates gut microbiota, innate immune response and health status of marron (Cherax cainii, Austin 2002) fed poultry-by-product and fishmeal based diets
    Article Snippet: Paragraph title: DNA extraction, PCR, and high throughput sequencing ... PCR master mixture was prepared as 50 µL final concentration containing 25 µL Hot Start 2X Master Mix (New England BioLabs Inc., Ipswich, MA, USA), two µL of template DNA, one µL of each forward and reverse V3–V4 sequencing primers, and 21 µL of nuclease-free water.

    Spectrophotometry:

    Article Title: Fermented Animal Source Protein as Substitution of Fishmeal on Intestinal Microbiota, Immune-Related Cytokines and Resistance to Vibrio mimicus in Freshwater Crayfish (Cherax cainii)
    Article Snippet: Bacterial DNA was extracted following manufacturer’s protocols and quantified by a NanoDrop Spectrophotometer (Thermo-Fisher Scientific, Waltham, MA, United States). .. PCR reactions were carried out in a total volume of 50 μL of master mixture, containing Hot Start Taq 2X Master Mix (New England BioLabs Inc., Ipswich, MA, United States) (25 μL), template DNA (2 μL), V3-V4 sequencing primers (1 μL each), and nuclease-free water (21 μL).

    Article Title: Antibiotic resistance pattern and virulence genes content in avian pathogenic Escherichia coli (APEC) from broiler chickens in Chitwan, Nepal
    Article Snippet: The quality of genomic DNA was checked by gel electrophoresis and measuring absorbance at A 260 / A 280 and A 260 / A 230 ratios using the MaestroNano spectrophotometer (MaestroGen; Model name: MN-913). .. The PCR was performed in 25 μL volume containing 12.5 μL Hot start Taq 2X master mix (BioLab Inc., New England), 1 μL each primer, 2 μL DNA template, and 8.5 μL nuclease free water.

    DNA Extraction:

    Article Title: New insights into valve-related intramural and intracellular bacterial diversity in infective endocarditis
    Article Snippet: Paragraph title: DNA extraction and 16S rDNA amplification ... Briefly, each 20 μL of PCR reaction mixture contained 20 ng of total or microbial-enriched DNA as a template, 10 μL 2x Hot Start Taq Master Mix (New England BioLabs), and 0.5 μM of each primer.

    Article Title: Fermented Animal Source Protein as Substitution of Fishmeal on Intestinal Microbiota, Immune-Related Cytokines and Resistance to Vibrio mimicus in Freshwater Crayfish (Cherax cainii)
    Article Snippet: Approximately 200 mg of distal intestinal content of marron were transferred into 1.5 mL eppendorf tubes and processed for DNA extraction using DNeasy Blood and Tissue Kit (Qiagen, Crawley, United Kindom). .. PCR reactions were carried out in a total volume of 50 μL of master mixture, containing Hot Start Taq 2X Master Mix (New England BioLabs Inc., Ipswich, MA, United States) (25 μL), template DNA (2 μL), V3-V4 sequencing primers (1 μL each), and nuclease-free water (21 μL).

    Article Title: Dietary supplementation of black soldier fly (Hermetica illucens) meal modulates gut microbiota, innate immune response and health status of marron (Cherax cainii, Austin 2002) fed poultry-by-product and fishmeal based diets
    Article Snippet: Paragraph title: DNA extraction, PCR, and high throughput sequencing ... PCR master mixture was prepared as 50 µL final concentration containing 25 µL Hot Start 2X Master Mix (New England BioLabs Inc., Ipswich, MA, USA), two µL of template DNA, one µL of each forward and reverse V3–V4 sequencing primers, and 21 µL of nuclease-free water.

    Concentration Assay:

    Article Title: The relationship between insecticide resistance, mosquito age and malaria prevalence in Anopheles gambiae s.l. from Guinea
    Article Snippet: .. At both LSHTM and CDC, PCR reactions were prepared using Hot Start Taq 2X Master Mix (New England Biolabs) with each reaction containing 12.5 µl of master mix, a final concentration of 1 µM of each primer, 2 µl template DNA, to a final reaction volume of 25 µl. .. Prepared reactions were run on a BIO-RAD QPCR system for 5 min at 95˚C, followed by 30 cycles of 95˚C for 30 sec, 52˚C for 30 sec and 72˚C for 1 min and a final extension of 72˚C for 5 min. PCR products were digested using the Alu I restriction enzyme through incubation at 37˚C for 16 hr followed by 65˚C for 20 min. DNA fragments were visualised on 2% Egel EX agarose gels (Invitrogen) with SYBR safe and an Invitrogen E-gel iBase Real-Time Transilluminator.

    Article Title: Dietary supplementation of black soldier fly (Hermetica illucens) meal modulates gut microbiota, innate immune response and health status of marron (Cherax cainii, Austin 2002) fed poultry-by-product and fishmeal based diets
    Article Snippet: .. PCR master mixture was prepared as 50 µL final concentration containing 25 µL Hot Start 2X Master Mix (New England BioLabs Inc., Ipswich, MA, USA), two µL of template DNA, one µL of each forward and reverse V3–V4 sequencing primers, and 21 µL of nuclease-free water. .. The PCR was performed using BioRad S100 Gradient Thermal Cycler (Bio-Rad Laboratories, Inc., Foster City, CA, USA), under the following conditions: 2 min initial denaturation at 94 °C; 30 cycles of denaturation (30 s at 94 °C), annealing (1 min at 55 °C), and extension (1 min at 68 °C); a final extension at 68 °C for 5 min and holding the temperature at 10 °C.

    Staining:

    Article Title: The Evolutionarily Conserved Cassette Exon 7b Drives ERG's Oncogenic Properties
    Article Snippet: Hot Start Taq 2× master mix (New England Biolabs) was used for standard PCR. .. Gel electrophoresis was carried out using 1.5% agarose gels stained with 5 μl Midori Green Advance DNA stain (Geneflow) for every 100 ml of TAE.

    Article Title: Antibiotic resistance pattern and virulence genes content in avian pathogenic Escherichia coli (APEC) from broiler chickens in Chitwan, Nepal
    Article Snippet: The PCR was performed in 25 μL volume containing 12.5 μL Hot start Taq 2X master mix (BioLab Inc., New England), 1 μL each primer, 2 μL DNA template, and 8.5 μL nuclease free water. .. All the PCR products were stained with ethidium bromide.

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    New England Biolabs one taq hot start 2x pcr master mix
    One Taq Hot Start 2x Pcr Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/one taq hot start 2x pcr master mix/product/New England Biolabs
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    one taq hot start 2x pcr master mix - by Bioz Stars, 2020-03
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