one shot top10  (Thermo Fisher)


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    Structured Review

    Thermo Fisher one shot top10
    One Shot Top10, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 170 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/one shot top10/product/Thermo Fisher
    Average 99 stars, based on 170 article reviews
    Price from $9.99 to $1999.99
    one shot top10 - by Bioz Stars, 2020-05
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: AQUA Cloning: A Versatile and Simple Enzyme-Free Cloning Approach
    Article Snippet: .. E . coli strains The following commercially available strains of E . coli were also used for AQUA Cloning: One Shot TOP10 (Invitrogen cat. No. C4040-03, competency: > 109 CFU/μg), NEB5α (NEB, cat. No. C2987I, competency: 1–3 x 109 CFU/μg), NEB10β (NEB, cat. No. C3019I, competency: 1–3 x 109 CFU/μg), BL21 (DE3) (NEB, cat. No. C2527I, competency: 1–5 x 107 CFU/μg), JM109 (Promega, cat. No. L2005, competency: 108 CFU/μg). .. Confocal imaging For confocal imaging, human embryonic kidney cells (HEK-293T) [ ], or NIH/3T3 fibroblasts cells (ATCC CRL-1658) were maintained in Dulbecco’s modified Eagle’s medium (PAN, cat. No. P03-0710) supplemented with 10% fetal bovine serum (PAN, cat. no. 1502-P123002, batch no. P123002), 100 U/mL penicillin and 0.1 mg/mL streptomycin (PAN).

    Article Title: α-Xylosidase plays essential roles in xyloglucan remodelling, maintenance of cell wall integrity, and seed germination in Arabidopsis thaliana
    Article Snippet: .. The product was cloned into the Gateway entry vector using the pENTR Directional TOPO Cloning Kit (Invitrogen, K2400-20SP) and transformed One Shot Chemically Competent Escherichia coli (Invitrogen, C4040-03). .. Kanamycin-resistant colonies were selected and plasmid DNA was prepared using the QIAprep Spin Miniprep Kit (Qiagen, 27104).

    Article Title: Predicting RNA hyper-editing with a novel tool when unambiguous alignment is impossible
    Article Snippet: .. Subsequently, 4 μ L of PCR product was cloned into One Shot®;TOP10 Chemically Competent E. coli cells using Zero Blunt®;TOPO®;PCR Cloning Kit from Invitrogen (catalog number: K2800J10), according to manufacturer’s guidelines. .. A total of 50 μ L solution containing the transformed cells were plated on kanamycin+agar plates.

    other:

    Article Title: Monoubiquitination of ASXLs controls the deubiquitinase activity of the tumor suppressor BAP1
    Article Snippet: Bacterial strains TOP10 chemically competent E.Coli bacteria (One Shot™ TOP10 Chemically Competent E. coli) were purchased from ThermoFisher (C404010).

    DNA Sequencing:

    Article Title: The polypeptide Syn67 interacts physically with human holocarboxylase synthetase, but is not a target for biotinylation
    Article Snippet: .. Eluted plasmids (10 µL) were used to transform One Shot TOP10 chemically competent E. coli cells (Invitrogen, Carlsbad, CA) for subsequent plasmid preparation (QIAprep Spin Miniprep kit, Qiagen, Valencia, CA) and DNA sequencing. ..

    Polymerase Chain Reaction:

    Article Title: Predicting RNA hyper-editing with a novel tool when unambiguous alignment is impossible
    Article Snippet: .. Subsequently, 4 μ L of PCR product was cloned into One Shot®;TOP10 Chemically Competent E. coli cells using Zero Blunt®;TOPO®;PCR Cloning Kit from Invitrogen (catalog number: K2800J10), according to manufacturer’s guidelines. .. A total of 50 μ L solution containing the transformed cells were plated on kanamycin+agar plates.

    Transformation Assay:

    Article Title: α-Xylosidase plays essential roles in xyloglucan remodelling, maintenance of cell wall integrity, and seed germination in Arabidopsis thaliana
    Article Snippet: .. The product was cloned into the Gateway entry vector using the pENTR Directional TOPO Cloning Kit (Invitrogen, K2400-20SP) and transformed One Shot Chemically Competent Escherichia coli (Invitrogen, C4040-03). .. Kanamycin-resistant colonies were selected and plasmid DNA was prepared using the QIAprep Spin Miniprep Kit (Qiagen, 27104).

    Article Title: Virus-Induced Gene Silencing as a Tool for Comparative Functional Studies in Thalictrum
    Article Snippet: .. The TRV2 plasmid and TdPDS fragment were digested with BamH1 and Xho1 (New England Biolabs, Ipswich, MA), ligated using T4 DNA ligase (Invitrogen, Carlsbad, CA) and transformed into One Shot® TOP10 Chemically Competent E. coli (Invitrogen, Carlsbad, CA). .. Colonies were selected on LB plates containing 50 µg/ml of Kanamycin and the presence of insert was confirmed by PCR with primers spanning the Multiple Cloning Site of pTRV2 (156 F: 5′- TTA CTC AAG GAA GCA CGA TGA GC -3′ and 156 R: 5′- GAA CCG TAG TTT AAT GTC TTC GGG -3′ ) .

    Article Title: Mössbauer Study and Modeling of Iron Import and Trafficking in Human Jurkat Cells
    Article Snippet: .. The resulting plasmid was transformed into OneShot TOP10 E. coli competent cells (Life Technologies), which were then plated and grown at 37 °C overnight on LB agar plates containing 100 μg/mL of ampicillin. .. Plasmids were purified from these E. coli cells using the PureLink Quick Plasmid Miniprep kit (Life Technologies) as per manufacturer's instructions.

    Article Title: Quorum-Sensing Systems LuxS/Autoinducer 2 and Com Regulate Streptococcus pneumoniae Biofilms in a Bioreactor with Living Cultures of Human Respiratory Cells
    Article Snippet: .. This gene was ligated into a previously KpnI-XbaI-digested pPP2 integrative plasmid ( ) using T4 DNA ligase (Promega) and transformed into One Shot TOP10 chemically competent Escherichia coli (Invitrogen). .. Transformants were selected on LB agar plates containing 100 μg/ml ampicillin and screened by colony PCR with primers comC-L and comC-R, and comC -bearing clones were confirmed by sequencing.

    Plasmid Preparation:

    Article Title: α-Xylosidase plays essential roles in xyloglucan remodelling, maintenance of cell wall integrity, and seed germination in Arabidopsis thaliana
    Article Snippet: .. The product was cloned into the Gateway entry vector using the pENTR Directional TOPO Cloning Kit (Invitrogen, K2400-20SP) and transformed One Shot Chemically Competent Escherichia coli (Invitrogen, C4040-03). .. Kanamycin-resistant colonies were selected and plasmid DNA was prepared using the QIAprep Spin Miniprep Kit (Qiagen, 27104).

    Article Title: Virus-Induced Gene Silencing as a Tool for Comparative Functional Studies in Thalictrum
    Article Snippet: .. The TRV2 plasmid and TdPDS fragment were digested with BamH1 and Xho1 (New England Biolabs, Ipswich, MA), ligated using T4 DNA ligase (Invitrogen, Carlsbad, CA) and transformed into One Shot® TOP10 Chemically Competent E. coli (Invitrogen, Carlsbad, CA). .. Colonies were selected on LB plates containing 50 µg/ml of Kanamycin and the presence of insert was confirmed by PCR with primers spanning the Multiple Cloning Site of pTRV2 (156 F: 5′- TTA CTC AAG GAA GCA CGA TGA GC -3′ and 156 R: 5′- GAA CCG TAG TTT AAT GTC TTC GGG -3′ ) .

    Article Title: Mössbauer Study and Modeling of Iron Import and Trafficking in Human Jurkat Cells
    Article Snippet: .. The resulting plasmid was transformed into OneShot TOP10 E. coli competent cells (Life Technologies), which were then plated and grown at 37 °C overnight on LB agar plates containing 100 μg/mL of ampicillin. .. Plasmids were purified from these E. coli cells using the PureLink Quick Plasmid Miniprep kit (Life Technologies) as per manufacturer's instructions.

    Article Title: The polypeptide Syn67 interacts physically with human holocarboxylase synthetase, but is not a target for biotinylation
    Article Snippet: .. Eluted plasmids (10 µL) were used to transform One Shot TOP10 chemically competent E. coli cells (Invitrogen, Carlsbad, CA) for subsequent plasmid preparation (QIAprep Spin Miniprep kit, Qiagen, Valencia, CA) and DNA sequencing. ..

    Article Title: Quorum-Sensing Systems LuxS/Autoinducer 2 and Com Regulate Streptococcus pneumoniae Biofilms in a Bioreactor with Living Cultures of Human Respiratory Cells
    Article Snippet: .. This gene was ligated into a previously KpnI-XbaI-digested pPP2 integrative plasmid ( ) using T4 DNA ligase (Promega) and transformed into One Shot TOP10 chemically competent Escherichia coli (Invitrogen). .. Transformants were selected on LB agar plates containing 100 μg/ml ampicillin and screened by colony PCR with primers comC-L and comC-R, and comC -bearing clones were confirmed by sequencing.

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  • 99
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