one shot top10 e  (Thermo Fisher)


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    Name:
    One Shot TOP10 Electrocomp E coli
    Description:
    One Shot TOP10 Electrocomp E coli are provided at a transformation efficiency of 1 x 1010 cfu µg supercoiled DNA and are ideal for high efficiency cloning and plasmid propagation They allow stable replication of high copy number plasmids The genotype of TOP10 cells is similar to that of the DH10B strain and offers the following features • hsdR for efficient transformation of unmethylated DNA from PCR amplifications• mcrA for efficient transformation of methylated DNA from genomic preparations• lacZΔM15 for blue white color screening of recombinant clones• endA1 for cleaner preparations of DNA get better results in downstream applications due to the elimination of nonspecific digestion by Endonuclease I• recA1 for reduced occurrence of nonspecific recombination in cloned DNAKit optionsOne Shot TOP10 Kits are available with either chemically competent or electrocompetent E coli to fit your specific transformation needs TOP10 E coli are also available in the high throughput MultiShot format
    Catalog Number:
    c404050
    Price:
    None
    Applications:
    Cloning|Electrocompetent Cells for Cloning|Transformation
    Category:
    Competent Cells Strains
    Buy from Supplier


    Structured Review

    Thermo Fisher one shot top10 e
    One Shot TOP10 Electrocomp E coli are provided at a transformation efficiency of 1 x 1010 cfu µg supercoiled DNA and are ideal for high efficiency cloning and plasmid propagation They allow stable replication of high copy number plasmids The genotype of TOP10 cells is similar to that of the DH10B strain and offers the following features • hsdR for efficient transformation of unmethylated DNA from PCR amplifications• mcrA for efficient transformation of methylated DNA from genomic preparations• lacZΔM15 for blue white color screening of recombinant clones• endA1 for cleaner preparations of DNA get better results in downstream applications due to the elimination of nonspecific digestion by Endonuclease I• recA1 for reduced occurrence of nonspecific recombination in cloned DNAKit optionsOne Shot TOP10 Kits are available with either chemically competent or electrocompetent E coli to fit your specific transformation needs TOP10 E coli are also available in the high throughput MultiShot format
    https://www.bioz.com/result/one shot top10 e/product/Thermo Fisher
    Average 95 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    one shot top10 e - by Bioz Stars, 2020-07
    95/100 stars

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    Related Articles

    Electroporation:

    Article Title: Sponge Microbiota Are a Reservoir of Functional Antibiotic Resistance Genes
    Article Snippet: .. At electroporation, 2 μl ligation mixture was added to 50 μl One Shot® TOP10 Electrocomp® E. coli cells (Thermo Fisher Scientific). ..

    Agarose Gel Electrophoresis:

    Article Title: Galactomannan Catabolism Conferred by a Polysaccharide Utilization Locus of Bacteroides ovatus
    Article Snippet: .. The presence of the anticipated deletion product was shown by agarose gel electrophoresis and the plasmid was transformed into OneShot® TOP10 ElectrocompTM E. coli cells (Life Technologies, Inc.), following the manufacturer's recommendations. .. The presence of the desired construct was verified by colony PCR using MyTaqTM HS DNA polymerase (Bioline, London, UK) and T7 primers, and by DNA sequencing also using T7 primers (Eurofins Genomics, Ebersberg, Germany).

    Clone Assay:

    Article Title: Insulin-like Growth Factor-binding Protein-5-induced Laminin ?1 Transcription Requires Filamin A *
    Article Snippet: .. The cloning reaction was transformed into One Shot TOP10 Escherichia coli (Invitrogen). .. Multiple clones were screened by PCR using GFP vector sequencing primers provided with the vector.

    Article Title: Defects of mitochondrial RNA turnover lead to the accumulation of double-stranded RNA in vivo
    Article Snippet: .. The PCR products were then cloned into pCRII-TOPO and transformed in One Shot TOP10 E . coli (Thermo Scientific) according to manufacturer’s instructions. .. The plasmids were purified and the insert was sequenced using M13 sequencing primers.

    Ligation:

    Article Title: Structure and expression of GSL1 and GSL2 genes encoding gibberellin stimulated-like proteins in diploid and highly heterozygous tetraploid potato reveals their highly conserved and essential status
    Article Snippet: .. One Shot® TOP10 Electrocomp™ E. coli Cells (Invitrogen) were transformed with DNA from blunt-end ligation reactions. .. Plasmid DNA from clones was isolated using High Pure Plasmid Isolation Kit (Roche Applied Science) and tested by restriction analysis using Hin dIII enzyme.

    Article Title: Sponge Microbiota Are a Reservoir of Functional Antibiotic Resistance Genes
    Article Snippet: .. At electroporation, 2 μl ligation mixture was added to 50 μl One Shot® TOP10 Electrocomp® E. coli cells (Thermo Fisher Scientific). ..

    Construct:

    Article Title: Autocatalytic Activation of the Furin Zymogen Requires Removal of the Emerging Enzyme's N-Terminus from the Active Site
    Article Snippet: .. One Shot TOP 10 E.coli cells (Invitrogen) were transformed with the recombinant prodomain construct. .. The expression of the recombinant protein was induced with 1 mM isopropyl-1-thio-β-D-galactopyranoside.

    Polymerase Chain Reaction:

    Article Title: Allele-specific expression at the androgen receptor alpha gene in a hybrid unisexual fish, the Amazon molly (Poecilia formosa)
    Article Snippet: .. Cleaned fragments were ligated with pCR™4-TOPO® TA Vector and transformed into One Shot TOP10 Electrocomp™ E . coli (Life Technologies). .. After positive kanamycin selection, fragments were re-amplified using T3/T7 PCR with MyTaqTM DNA polymerase (Bioline).

    Article Title: Defects of mitochondrial RNA turnover lead to the accumulation of double-stranded RNA in vivo
    Article Snippet: .. The PCR products were then cloned into pCRII-TOPO and transformed in One Shot TOP10 E . coli (Thermo Scientific) according to manufacturer’s instructions. .. The plasmids were purified and the insert was sequenced using M13 sequencing primers.

    Transformation Assay:

    Article Title: Structure and expression of GSL1 and GSL2 genes encoding gibberellin stimulated-like proteins in diploid and highly heterozygous tetraploid potato reveals their highly conserved and essential status
    Article Snippet: .. One Shot® TOP10 Electrocomp™ E. coli Cells (Invitrogen) were transformed with DNA from blunt-end ligation reactions. .. Plasmid DNA from clones was isolated using High Pure Plasmid Isolation Kit (Roche Applied Science) and tested by restriction analysis using Hin dIII enzyme.

    Article Title: Complementary expression of EphA7 and SCO-spondin during posterior commissure development
    Article Snippet: .. One Shot TOP10 Escherichia coli (Invitrogen, Carlsbad, CA, USA) were transformed with this vector. .. Posterior purification of the plasmid was performed with the E.Z.N.A.

    Article Title: Galactomannan Catabolism Conferred by a Polysaccharide Utilization Locus of Bacteroides ovatus
    Article Snippet: .. The presence of the anticipated deletion product was shown by agarose gel electrophoresis and the plasmid was transformed into OneShot® TOP10 ElectrocompTM E. coli cells (Life Technologies, Inc.), following the manufacturer's recommendations. .. The presence of the desired construct was verified by colony PCR using MyTaqTM HS DNA polymerase (Bioline, London, UK) and T7 primers, and by DNA sequencing also using T7 primers (Eurofins Genomics, Ebersberg, Germany).

    Article Title: Allele-specific expression at the androgen receptor alpha gene in a hybrid unisexual fish, the Amazon molly (Poecilia formosa)
    Article Snippet: .. Cleaned fragments were ligated with pCR™4-TOPO® TA Vector and transformed into One Shot TOP10 Electrocomp™ E . coli (Life Technologies). .. After positive kanamycin selection, fragments were re-amplified using T3/T7 PCR with MyTaqTM DNA polymerase (Bioline).

    Article Title: Autocatalytic Activation of the Furin Zymogen Requires Removal of the Emerging Enzyme's N-Terminus from the Active Site
    Article Snippet: .. One Shot TOP 10 E.coli cells (Invitrogen) were transformed with the recombinant prodomain construct. .. The expression of the recombinant protein was induced with 1 mM isopropyl-1-thio-β-D-galactopyranoside.

    Article Title: Insulin-like Growth Factor-binding Protein-5-induced Laminin ?1 Transcription Requires Filamin A *
    Article Snippet: .. The cloning reaction was transformed into One Shot TOP10 Escherichia coli (Invitrogen). .. Multiple clones were screened by PCR using GFP vector sequencing primers provided with the vector.

    Article Title: Defects of mitochondrial RNA turnover lead to the accumulation of double-stranded RNA in vivo
    Article Snippet: .. The PCR products were then cloned into pCRII-TOPO and transformed in One Shot TOP10 E . coli (Thermo Scientific) according to manufacturer’s instructions. .. The plasmids were purified and the insert was sequenced using M13 sequencing primers.

    Recombinant:

    Article Title: Autocatalytic Activation of the Furin Zymogen Requires Removal of the Emerging Enzyme's N-Terminus from the Active Site
    Article Snippet: .. One Shot TOP 10 E.coli cells (Invitrogen) were transformed with the recombinant prodomain construct. .. The expression of the recombinant protein was induced with 1 mM isopropyl-1-thio-β-D-galactopyranoside.

    Plasmid Preparation:

    Article Title: Complementary expression of EphA7 and SCO-spondin during posterior commissure development
    Article Snippet: .. One Shot TOP10 Escherichia coli (Invitrogen, Carlsbad, CA, USA) were transformed with this vector. .. Posterior purification of the plasmid was performed with the E.Z.N.A.

    Article Title: Galactomannan Catabolism Conferred by a Polysaccharide Utilization Locus of Bacteroides ovatus
    Article Snippet: .. The presence of the anticipated deletion product was shown by agarose gel electrophoresis and the plasmid was transformed into OneShot® TOP10 ElectrocompTM E. coli cells (Life Technologies, Inc.), following the manufacturer's recommendations. .. The presence of the desired construct was verified by colony PCR using MyTaqTM HS DNA polymerase (Bioline, London, UK) and T7 primers, and by DNA sequencing also using T7 primers (Eurofins Genomics, Ebersberg, Germany).

    Article Title: Allele-specific expression at the androgen receptor alpha gene in a hybrid unisexual fish, the Amazon molly (Poecilia formosa)
    Article Snippet: .. Cleaned fragments were ligated with pCR™4-TOPO® TA Vector and transformed into One Shot TOP10 Electrocomp™ E . coli (Life Technologies). .. After positive kanamycin selection, fragments were re-amplified using T3/T7 PCR with MyTaqTM DNA polymerase (Bioline).

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  • Bioz Stars
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  • 95
    Thermo Fisher one shot top10 e
    One Shot Top10 E, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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