one shot omnimax 2 t1  (Thermo Fisher)


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    Structured Review

    Thermo Fisher one shot omnimax 2 t1
    One Shot Omnimax 2 T1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 82/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/one shot omnimax 2 t1/product/Thermo Fisher
    Average 82 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    one shot omnimax 2 t1 - by Bioz Stars, 2020-01
    82/100 stars

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    Related Articles

    Clone Assay:

    Article Title: The plasmid-encoded transcription factor ArdK contributes to the repression of the IMP-6 metallo-β-lactamase gene blaIMP-6, leading to a carbapenem-susceptible phenotype in the blaIMP-6-positive Escherichia coli strain A56-1S
    Article Snippet: The PCR products from the region upstream of bla IMP-6 were inserted into a linearized pACYC184 vector using the In-Fusion HD Cloning Kit (TaKaRa). .. The cloned plasmids were propagated in One Shot OmniMAX 2 T1 Chemically Competent E . coli (Thermo Fisher Scientific) and purified using the PureYield Plasmid Miniprep System (Promega, Madison, WI, USA). .. These plasmids were transformed into OmniMAX bacteria, and transformants carrying both UPS/UPR and intact/mutated ardK were selected using chloramphenicol and kanamycin.

    Amplification:

    Article Title: The plasmid-encoded transcription factor ArdK contributes to the repression of the IMP-6 metallo-β-lactamase gene blaIMP-6, leading to a carbapenem-susceptible phenotype in the blaIMP-6-positive Escherichia coli strain A56-1S
    Article Snippet: DNA fragments from the region upstream of bla IMP-6 were amplified from pA56-1S (UPS; 3,524 bp; 3,193–6,716 nt in GenBank ID: AP018362) genomic DNA using IntI1_pACYC184_F and R, and from pA56-1R (UPR; 2,246 bp; 51,305–53,550 nt in GenBank ID: AP018363) genomic DNA using ardK _cloning_F and IntI1_pACYC184_R. .. The cloned plasmids were propagated in One Shot OmniMAX 2 T1 Chemically Competent E . coli (Thermo Fisher Scientific) and purified using the PureYield Plasmid Miniprep System (Promega, Madison, WI, USA).

    Isolation:

    Article Title: The plasmid-encoded transcription factor ArdK contributes to the repression of the IMP-6 metallo-β-lactamase gene blaIMP-6, leading to a carbapenem-susceptible phenotype in the blaIMP-6-positive Escherichia coli strain A56-1S
    Article Snippet: The cloned plasmids were propagated in One Shot OmniMAX 2 T1 Chemically Competent E . coli (Thermo Fisher Scientific) and purified using the PureYield Plasmid Miniprep System (Promega, Madison, WI, USA). .. These plasmids were transformed into OmniMAX bacteria, and transformants carrying both UPS/UPR and intact/mutated ardK were selected using chloramphenicol and kanamycin.

    Purification:

    Article Title: The plasmid-encoded transcription factor ArdK contributes to the repression of the IMP-6 metallo-β-lactamase gene blaIMP-6, leading to a carbapenem-susceptible phenotype in the blaIMP-6-positive Escherichia coli strain A56-1S
    Article Snippet: The PCR products from the region upstream of bla IMP-6 were inserted into a linearized pACYC184 vector using the In-Fusion HD Cloning Kit (TaKaRa). .. The cloned plasmids were propagated in One Shot OmniMAX 2 T1 Chemically Competent E . coli (Thermo Fisher Scientific) and purified using the PureYield Plasmid Miniprep System (Promega, Madison, WI, USA). .. These plasmids were transformed into OmniMAX bacteria, and transformants carrying both UPS/UPR and intact/mutated ardK were selected using chloramphenicol and kanamycin.

    Polymerase Chain Reaction:

    Article Title: The plasmid-encoded transcription factor ArdK contributes to the repression of the IMP-6 metallo-β-lactamase gene blaIMP-6, leading to a carbapenem-susceptible phenotype in the blaIMP-6-positive Escherichia coli strain A56-1S
    Article Snippet: The PCR products from the region upstream of bla IMP-6 were inserted into a linearized pACYC184 vector using the In-Fusion HD Cloning Kit (TaKaRa). .. The cloned plasmids were propagated in One Shot OmniMAX 2 T1 Chemically Competent E . coli (Thermo Fisher Scientific) and purified using the PureYield Plasmid Miniprep System (Promega, Madison, WI, USA).

    Plasmid Preparation:

    Article Title: The plasmid-encoded transcription factor ArdK contributes to the repression of the IMP-6 metallo-β-lactamase gene blaIMP-6, leading to a carbapenem-susceptible phenotype in the blaIMP-6-positive Escherichia coli strain A56-1S
    Article Snippet: The PCR products from the region upstream of bla IMP-6 were inserted into a linearized pACYC184 vector using the In-Fusion HD Cloning Kit (TaKaRa). .. The cloned plasmids were propagated in One Shot OmniMAX 2 T1 Chemically Competent E . coli (Thermo Fisher Scientific) and purified using the PureYield Plasmid Miniprep System (Promega, Madison, WI, USA). .. These plasmids were transformed into OmniMAX bacteria, and transformants carrying both UPS/UPR and intact/mutated ardK were selected using chloramphenicol and kanamycin.

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  • Team
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  • 82
    Thermo Fisher one shot omnimax 2 t1
    One Shot Omnimax 2 T1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 82/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/one shot omnimax 2 t1/product/Thermo Fisher
    Average 82 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    one shot omnimax 2 t1 - by Bioz Stars, 2020-01
    82/100 stars
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