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Agilent technologies one color rna spike in mix
One Color Rna Spike In Mix, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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one color rna spike in mix - by Bioz Stars, 2019-10
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RNA Extraction:

Article Title: Transcriptomics of the Interaction between the Monopartite Phloem-Limited Geminivirus Tomato Yellow Leaf Curl Sardinia Virus and Solanum lycopersicum Highlights a Role for Plant Hormones, Autophagy and Plant Immune System Fine Tuning during Infection
Article Snippet: Paragraph title: RNA extraction and microarray experiment ... RNA integrity was examined with the Experion Automated Electrophoresis Station (Bio-Rad). cDNA synthesis, hybridization, washing and scanning were performed by IMGM Laboratories ( www.imgm.com ) as follows: 500 ng of total RNA were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), which serve as internal labelling control for linearity, sensitivity and accuracy.

Amplification:

Article Title: Custom microarray construction and analysis for determining potential biomarkers of subchronic androgen exposure in the Eastern Mosquitofish (Gambusia holbrooki)
Article Snippet: For amplification and labeling, the Quick Amp Labeling kit (Agilent, Santa Clara, USA) was utilised with adjustments made for a half reaction, one color (Cy3) protocol. .. In brief, T7 primers were annealed to 1000 ng RNA with the addition of RNA spike-in controls (One color RNA spike-in mix; Agilent, Santa Clara, USA) by incubation at 65°C for 10 minutes. cDNA synthesis using MMLV-RT was conducted at 40°C for 2 hours followed by 65°C for 15 minutes. cDNA was reverse-transcribed into cRNA using a T7 RNA polymerase with the addition of Cy3 to the reaction.

Article Title: Convergent Differential Regulation of Parvalbumin in the Brains of Vocal Learners
Article Snippet: Five nanograms of total RNA were linearly amplified as cDNA using the uMACS SuperAmp Kit (Miltenyi Biotec, Germany). .. Just before cDNA amplification, a 1∶200000 dilution of Agilent One Color RNA Spike-In Mix (Agilent Technologies) was added to five nanograms of total RNA from each sample. .. The Spike-in recognizes only control non-vertebrate oligos on the array, which allows us to detect amplification and hybridization artifacts.

In Vitro:

Article Title: Altered Gene Expression in Prefrontal Cortex of a Fabry Disease Mouse Model
Article Snippet: Samples were analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. Following cRNA clean-up and quantification, Cyanine-3-labeled cRNA samples were fragmented and prepared for one-color-based hybridization (Gene Expression Hybridization Kit, Agilent Technologies) and hybridized at 65° C for 17 h on Agilent SurePrint G3 Mouse GE 8x60K Microarrays.

Article Title: Altered Gene Expression in Prefrontal Cortex of a Fabry Disease Mouse Model
Article Snippet: Samples were analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. Following cRNA clean-up and quantification, Cyanine-3-labeled cRNA samples were fragmented and prepared for one-color-based hybridization (Gene Expression Hybridization Kit, Agilent Technologies) and hybridized at 65° C for 17 h on Agilent SurePrint G3 Mouse GE 8x60K Microarrays.

Article Title: Signatures of Altered Gene Expression in Dorsal Root Ganglia of a Fabry Disease Mouse Model
Article Snippet: Samples were analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio, and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. Following cRNA clean-up and quantification, Cyanine-3-labeled cRNA samples were fragmented and prepared for one-color-based hybridization (Gene Expression Hybridization Kit, Agilent Technologies) and hybridized at 65°C for 17 h on Agilent SurePrint G3 Mouse GE 8 × 60K Microarrays.

Article Title: Signatures of Altered Gene Expression in Dorsal Root Ganglia of a Fabry Disease Mouse Model
Article Snippet: Samples were analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio, and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. Following cRNA clean-up and quantification, Cyanine-3-labeled cRNA samples were fragmented and prepared for one-color-based hybridization (Gene Expression Hybridization Kit, Agilent Technologies) and hybridized at 65°C for 17 h on Agilent SurePrint G3 Mouse GE 8 × 60K Microarrays.

Article Title: Integrated omics approaches provide strategies for rapid erythromycin yield increase in Saccharopolyspora erythraea
Article Snippet: Sample labelling and hybridization were performed at IMGM (Germany). .. 300 ng of total RNA was spiked using One-Color RNA Spike-In Mix (Agilent Technologies) and subjected to reverse transcription, subsequent in vitro transcription, Cy3 labelling using Full Spectrum™ MultiStart Primers for T7 IVT (System Biosciences) and One-Color Quick-Amp Labelling Kit (Agilent Technologies) following manufacturer’s protocols. cRNA yield and integrity were determined using NanoDrop ND-1000 (Thermo Scientific) and 2100 Bioanalyzer (Agilent Technologies). cRNA was cleaned and hybridized to microarrays using Gene Expression Hybridization Kit (Agilent Technologies) following manufacturer’s protocols. .. Hybridized microarrays were washed using Gene Expression Wash Buffers (Agilent Technologies) followed by drying with acetonitrile.

Article Title: Transcriptomics of the Interaction between the Monopartite Phloem-Limited Geminivirus Tomato Yellow Leaf Curl Sardinia Virus and Solanum lycopersicum Highlights a Role for Plant Hormones, Autophagy and Plant Immune System Fine Tuning during Infection
Article Snippet: RNA concentration and purity were determined with the NanoDrop ND-1000 spectrophotometer (Thermo Scientific, Wilmington, USA). .. RNA integrity was examined with the Experion Automated Electrophoresis Station (Bio-Rad). cDNA synthesis, hybridization, washing and scanning were performed by IMGM Laboratories ( www.imgm.com ) as follows: 500 ng of total RNA were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), which serve as internal labelling control for linearity, sensitivity and accuracy. .. The spiked total RNA was reverse transcribed into cDNA and then converted into labelled cRNA by in vitro transcription (Quick-Amp Labeling Kit One-Color, Agilent Technologies) incorporating Cyanine-3-CTP. cRNA concentration (ng/µl), RNA absorbance ratio (260 nm/280 nm) and Cyanine-3 dye concentration (pmol/µl) were checked for all cRNA samples using the NanoDrop ND-1000 UV-VIS spectrophotometer.

Fluorescence:

Article Title: Altered Gene Expression in Prefrontal Cortex of a Fabry Disease Mouse Model
Article Snippet: For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. After hybridization, microarrays were washed with increasing stringency using Triton X-102 supplemented Gene Expression Wash Buffers (Agilent Technologies) followed by drying with acetonitrile (Sigma).

Article Title: Altered Gene Expression in Prefrontal Cortex of a Fabry Disease Mouse Model
Article Snippet: For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. After hybridization, microarrays were washed with increasing stringency using Triton X-102 supplemented Gene Expression Wash Buffers (Agilent Technologies) followed by drying with acetonitrile (Sigma).

Article Title: Signatures of Altered Gene Expression in Dorsal Root Ganglia of a Fabry Disease Mouse Model
Article Snippet: For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio, and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. After hybridization, microarrays were washed with increasing stringency using Triton X-102 supplemented Gene Expression Wash Buffers (Agilent Technologies) followed by drying with acetonitrile (Sigma).

Article Title: Signatures of Altered Gene Expression in Dorsal Root Ganglia of a Fabry Disease Mouse Model
Article Snippet: For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio, and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. After hybridization, microarrays were washed with increasing stringency using Triton X-102 supplemented Gene Expression Wash Buffers (Agilent Technologies) followed by drying with acetonitrile (Sigma).

Synthesized:

Article Title: Altered Gene Expression in Prefrontal Cortex of a Fabry Disease Mouse Model
Article Snippet: Samples were analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. Following cRNA clean-up and quantification, Cyanine-3-labeled cRNA samples were fragmented and prepared for one-color-based hybridization (Gene Expression Hybridization Kit, Agilent Technologies) and hybridized at 65° C for 17 h on Agilent SurePrint G3 Mouse GE 8x60K Microarrays.

Article Title: Altered Gene Expression in Prefrontal Cortex of a Fabry Disease Mouse Model
Article Snippet: Samples were analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. Following cRNA clean-up and quantification, Cyanine-3-labeled cRNA samples were fragmented and prepared for one-color-based hybridization (Gene Expression Hybridization Kit, Agilent Technologies) and hybridized at 65° C for 17 h on Agilent SurePrint G3 Mouse GE 8x60K Microarrays.

Article Title: Signatures of Altered Gene Expression in Dorsal Root Ganglia of a Fabry Disease Mouse Model
Article Snippet: Samples were analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio, and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. Following cRNA clean-up and quantification, Cyanine-3-labeled cRNA samples were fragmented and prepared for one-color-based hybridization (Gene Expression Hybridization Kit, Agilent Technologies) and hybridized at 65°C for 17 h on Agilent SurePrint G3 Mouse GE 8 × 60K Microarrays.

Article Title: Signatures of Altered Gene Expression in Dorsal Root Ganglia of a Fabry Disease Mouse Model
Article Snippet: Samples were analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio, and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. Following cRNA clean-up and quantification, Cyanine-3-labeled cRNA samples were fragmented and prepared for one-color-based hybridization (Gene Expression Hybridization Kit, Agilent Technologies) and hybridized at 65°C for 17 h on Agilent SurePrint G3 Mouse GE 8 × 60K Microarrays.

Article Title: Convergent Differential Regulation of Parvalbumin in the Brains of Vocal Learners
Article Snippet: Just before cDNA amplification, a 1∶200000 dilution of Agilent One Color RNA Spike-In Mix (Agilent Technologies) was added to five nanograms of total RNA from each sample. .. Samples were linearly amplified as cDNA and labeled with Cy3-dCTP using the uMACS SuperAmp Kit (Miltenyi Biotec).

Article Title: Transcriptomics of the Interaction between the Monopartite Phloem-Limited Geminivirus Tomato Yellow Leaf Curl Sardinia Virus and Solanum lycopersicum Highlights a Role for Plant Hormones, Autophagy and Plant Immune System Fine Tuning during Infection
Article Snippet: RNA concentration and purity were determined with the NanoDrop ND-1000 spectrophotometer (Thermo Scientific, Wilmington, USA). .. RNA integrity was examined with the Experion Automated Electrophoresis Station (Bio-Rad). cDNA synthesis, hybridization, washing and scanning were performed by IMGM Laboratories ( www.imgm.com ) as follows: 500 ng of total RNA were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), which serve as internal labelling control for linearity, sensitivity and accuracy. .. The spiked total RNA was reverse transcribed into cDNA and then converted into labelled cRNA by in vitro transcription (Quick-Amp Labeling Kit One-Color, Agilent Technologies) incorporating Cyanine-3-CTP. cRNA concentration (ng/µl), RNA absorbance ratio (260 nm/280 nm) and Cyanine-3 dye concentration (pmol/µl) were checked for all cRNA samples using the NanoDrop ND-1000 UV-VIS spectrophotometer.

Isolation:

Article Title: Identifying Reliable Diagnostic/Predictive Biomarkers for Rheumatoid Arthritis
Article Snippet: Total RNA isolation, purity and integrity: Total RNA was isolated using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions. .. Total RNA (100 ng) was spiked with in vitro–synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies, Santa Clara, CA, USA).

Article Title: Altered Gene Expression in Prefrontal Cortex of a Fabry Disease Mouse Model
Article Snippet: Total RNA including small RNAs was isolated using the miRNeasy Mini Kit (Qiagen) according to the manufacturer's instructions and eluted in 40 μl RNase-free water. .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies).

Article Title: Altered Gene Expression in Prefrontal Cortex of a Fabry Disease Mouse Model
Article Snippet: Total RNA including small RNAs was isolated using the miRNeasy Mini Kit (Qiagen) according to the manufacturer's instructions and eluted in 40 μl RNase-free water. .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies).

Article Title: Signatures of Altered Gene Expression in Dorsal Root Ganglia of a Fabry Disease Mouse Model
Article Snippet: Total RNA including small RNAs was isolated using the miRNeasy Mini Kit (Qiagen) according to the manufacturer's instructions and eluted in 40 μl RNase-free water. .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio, and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies).

Article Title: Signatures of Altered Gene Expression in Dorsal Root Ganglia of a Fabry Disease Mouse Model
Article Snippet: Total RNA including small RNAs was isolated using the miRNeasy Mini Kit (Qiagen) according to the manufacturer's instructions and eluted in 40 μl RNase-free water. .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio, and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies).

Article Title: Convergent Differential Regulation of Parvalbumin in the Brains of Vocal Learners
Article Snippet: Paragraph title: Laser captured microdissection (LCM), RNA isolation and cDNA synthesis ... Just before cDNA amplification, a 1∶200000 dilution of Agilent One Color RNA Spike-In Mix (Agilent Technologies) was added to five nanograms of total RNA from each sample.

Article Title: Integrated omics approaches provide strategies for rapid erythromycin yield increase in Saccharopolyspora erythraea
Article Snippet: Cultures were fixed and RNA was isolated as described previously [ ]. .. 300 ng of total RNA was spiked using One-Color RNA Spike-In Mix (Agilent Technologies) and subjected to reverse transcription, subsequent in vitro transcription, Cy3 labelling using Full Spectrum™ MultiStart Primers for T7 IVT (System Biosciences) and One-Color Quick-Amp Labelling Kit (Agilent Technologies) following manufacturer’s protocols. cRNA yield and integrity were determined using NanoDrop ND-1000 (Thermo Scientific) and 2100 Bioanalyzer (Agilent Technologies). cRNA was cleaned and hybridized to microarrays using Gene Expression Hybridization Kit (Agilent Technologies) following manufacturer’s protocols.

Laser Capture Microdissection:

Article Title: Convergent Differential Regulation of Parvalbumin in the Brains of Vocal Learners
Article Snippet: Paragraph title: Laser captured microdissection (LCM), RNA isolation and cDNA synthesis ... Just before cDNA amplification, a 1∶200000 dilution of Agilent One Color RNA Spike-In Mix (Agilent Technologies) was added to five nanograms of total RNA from each sample.

Spectrophotometry:

Article Title: Transcriptomics of the Interaction between the Monopartite Phloem-Limited Geminivirus Tomato Yellow Leaf Curl Sardinia Virus and Solanum lycopersicum Highlights a Role for Plant Hormones, Autophagy and Plant Immune System Fine Tuning during Infection
Article Snippet: RNA concentration and purity were determined with the NanoDrop ND-1000 spectrophotometer (Thermo Scientific, Wilmington, USA). .. RNA integrity was examined with the Experion Automated Electrophoresis Station (Bio-Rad). cDNA synthesis, hybridization, washing and scanning were performed by IMGM Laboratories ( www.imgm.com ) as follows: 500 ng of total RNA were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), which serve as internal labelling control for linearity, sensitivity and accuracy.

Purification:

Article Title: Custom microarray construction and analysis for determining potential biomarkers of subchronic androgen exposure in the Eastern Mosquitofish (Gambusia holbrooki)
Article Snippet: In brief, T7 primers were annealed to 1000 ng RNA with the addition of RNA spike-in controls (One color RNA spike-in mix; Agilent, Santa Clara, USA) by incubation at 65°C for 10 minutes. cDNA synthesis using MMLV-RT was conducted at 40°C for 2 hours followed by 65°C for 15 minutes. cDNA was reverse-transcribed into cRNA using a T7 RNA polymerase with the addition of Cy3 to the reaction. .. In brief, T7 primers were annealed to 1000 ng RNA with the addition of RNA spike-in controls (One color RNA spike-in mix; Agilent, Santa Clara, USA) by incubation at 65°C for 10 minutes. cDNA synthesis using MMLV-RT was conducted at 40°C for 2 hours followed by 65°C for 15 minutes. cDNA was reverse-transcribed into cRNA using a T7 RNA polymerase with the addition of Cy3 to the reaction.

Article Title: Integrated omics approaches provide strategies for rapid erythromycin yield increase in Saccharopolyspora erythraea
Article Snippet: RNA was purified using Rneasy MinElute (Qiagen) and the quality and quantity were controlled by 2100 Bioanalyzer and RNA 6000 Nano LabChip Kit (Agilent Technologies). .. 300 ng of total RNA was spiked using One-Color RNA Spike-In Mix (Agilent Technologies) and subjected to reverse transcription, subsequent in vitro transcription, Cy3 labelling using Full Spectrum™ MultiStart Primers for T7 IVT (System Biosciences) and One-Color Quick-Amp Labelling Kit (Agilent Technologies) following manufacturer’s protocols. cRNA yield and integrity were determined using NanoDrop ND-1000 (Thermo Scientific) and 2100 Bioanalyzer (Agilent Technologies). cRNA was cleaned and hybridized to microarrays using Gene Expression Hybridization Kit (Agilent Technologies) following manufacturer’s protocols.

Electrophoresis:

Article Title: Transcriptomics of the Interaction between the Monopartite Phloem-Limited Geminivirus Tomato Yellow Leaf Curl Sardinia Virus and Solanum lycopersicum Highlights a Role for Plant Hormones, Autophagy and Plant Immune System Fine Tuning during Infection
Article Snippet: RNA concentration and purity were determined with the NanoDrop ND-1000 spectrophotometer (Thermo Scientific, Wilmington, USA). .. RNA integrity was examined with the Experion Automated Electrophoresis Station (Bio-Rad). cDNA synthesis, hybridization, washing and scanning were performed by IMGM Laboratories ( www.imgm.com ) as follows: 500 ng of total RNA were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), which serve as internal labelling control for linearity, sensitivity and accuracy. .. The spiked total RNA was reverse transcribed into cDNA and then converted into labelled cRNA by in vitro transcription (Quick-Amp Labeling Kit One-Color, Agilent Technologies) incorporating Cyanine-3-CTP. cRNA concentration (ng/µl), RNA absorbance ratio (260 nm/280 nm) and Cyanine-3 dye concentration (pmol/µl) were checked for all cRNA samples using the NanoDrop ND-1000 UV-VIS spectrophotometer.

Concentration Assay:

Article Title: Custom microarray construction and analysis for determining potential biomarkers of subchronic androgen exposure in the Eastern Mosquitofish (Gambusia holbrooki)
Article Snippet: In brief, T7 primers were annealed to 1000 ng RNA with the addition of RNA spike-in controls (One color RNA spike-in mix; Agilent, Santa Clara, USA) by incubation at 65°C for 10 minutes. cDNA synthesis using MMLV-RT was conducted at 40°C for 2 hours followed by 65°C for 15 minutes. cDNA was reverse-transcribed into cRNA using a T7 RNA polymerase with the addition of Cy3 to the reaction. .. Cy3-labelled cRNA was purified using the RNeasy kit (QIAGEN, Hilden, Germany) and eluted in nuclease-free water.

Article Title: Altered Gene Expression in Prefrontal Cortex of a Fabry Disease Mouse Model
Article Snippet: Samples were analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. Following cRNA clean-up and quantification, Cyanine-3-labeled cRNA samples were fragmented and prepared for one-color-based hybridization (Gene Expression Hybridization Kit, Agilent Technologies) and hybridized at 65° C for 17 h on Agilent SurePrint G3 Mouse GE 8x60K Microarrays.

Article Title: Altered Gene Expression in Prefrontal Cortex of a Fabry Disease Mouse Model
Article Snippet: Samples were analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. Following cRNA clean-up and quantification, Cyanine-3-labeled cRNA samples were fragmented and prepared for one-color-based hybridization (Gene Expression Hybridization Kit, Agilent Technologies) and hybridized at 65° C for 17 h on Agilent SurePrint G3 Mouse GE 8x60K Microarrays.

Article Title: Signatures of Altered Gene Expression in Dorsal Root Ganglia of a Fabry Disease Mouse Model
Article Snippet: Samples were analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio, and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. Following cRNA clean-up and quantification, Cyanine-3-labeled cRNA samples were fragmented and prepared for one-color-based hybridization (Gene Expression Hybridization Kit, Agilent Technologies) and hybridized at 65°C for 17 h on Agilent SurePrint G3 Mouse GE 8 × 60K Microarrays.

Article Title: Signatures of Altered Gene Expression in Dorsal Root Ganglia of a Fabry Disease Mouse Model
Article Snippet: Samples were analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio, and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. Following cRNA clean-up and quantification, Cyanine-3-labeled cRNA samples were fragmented and prepared for one-color-based hybridization (Gene Expression Hybridization Kit, Agilent Technologies) and hybridized at 65°C for 17 h on Agilent SurePrint G3 Mouse GE 8 × 60K Microarrays.

Article Title: Convergent Differential Regulation of Parvalbumin in the Brains of Vocal Learners
Article Snippet: The concentration and integrity of total RNA were measured on a 2100 Bioanalyzer (Agilent Technologies, USA) using the RNA Pico 6000 kit according to manufacturer instructions (Agilent Technologies). .. Just before cDNA amplification, a 1∶200000 dilution of Agilent One Color RNA Spike-In Mix (Agilent Technologies) was added to five nanograms of total RNA from each sample.

Article Title: Transcriptomics of the Interaction between the Monopartite Phloem-Limited Geminivirus Tomato Yellow Leaf Curl Sardinia Virus and Solanum lycopersicum Highlights a Role for Plant Hormones, Autophagy and Plant Immune System Fine Tuning during Infection
Article Snippet: RNA concentration and purity were determined with the NanoDrop ND-1000 spectrophotometer (Thermo Scientific, Wilmington, USA). .. RNA integrity was examined with the Experion Automated Electrophoresis Station (Bio-Rad). cDNA synthesis, hybridization, washing and scanning were performed by IMGM Laboratories ( www.imgm.com ) as follows: 500 ng of total RNA were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), which serve as internal labelling control for linearity, sensitivity and accuracy.

Incubation:

Article Title: Custom microarray construction and analysis for determining potential biomarkers of subchronic androgen exposure in the Eastern Mosquitofish (Gambusia holbrooki)
Article Snippet: For amplification and labeling, the Quick Amp Labeling kit (Agilent, Santa Clara, USA) was utilised with adjustments made for a half reaction, one color (Cy3) protocol. .. In brief, T7 primers were annealed to 1000 ng RNA with the addition of RNA spike-in controls (One color RNA spike-in mix; Agilent, Santa Clara, USA) by incubation at 65°C for 10 minutes. cDNA synthesis using MMLV-RT was conducted at 40°C for 2 hours followed by 65°C for 15 minutes. cDNA was reverse-transcribed into cRNA using a T7 RNA polymerase with the addition of Cy3 to the reaction. .. In vitro transcription proceeded at 40°C for 2 hours.

Activity Assay:

Article Title: Custom microarray construction and analysis for determining potential biomarkers of subchronic androgen exposure in the Eastern Mosquitofish (Gambusia holbrooki)
Article Snippet: In brief, T7 primers were annealed to 1000 ng RNA with the addition of RNA spike-in controls (One color RNA spike-in mix; Agilent, Santa Clara, USA) by incubation at 65°C for 10 minutes. cDNA synthesis using MMLV-RT was conducted at 40°C for 2 hours followed by 65°C for 15 minutes. cDNA was reverse-transcribed into cRNA using a T7 RNA polymerase with the addition of Cy3 to the reaction. .. Cy3-labelled cRNA was purified using the RNeasy kit (QIAGEN, Hilden, Germany) and eluted in nuclease-free water.

Labeling:

Article Title: Custom microarray construction and analysis for determining potential biomarkers of subchronic androgen exposure in the Eastern Mosquitofish (Gambusia holbrooki)
Article Snippet: Paragraph title: Microarray labeling and hybridization ... In brief, T7 primers were annealed to 1000 ng RNA with the addition of RNA spike-in controls (One color RNA spike-in mix; Agilent, Santa Clara, USA) by incubation at 65°C for 10 minutes. cDNA synthesis using MMLV-RT was conducted at 40°C for 2 hours followed by 65°C for 15 minutes. cDNA was reverse-transcribed into cRNA using a T7 RNA polymerase with the addition of Cy3 to the reaction.

Article Title: Altered Gene Expression in Prefrontal Cortex of a Fabry Disease Mouse Model
Article Snippet: Samples were analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. Following cRNA clean-up and quantification, Cyanine-3-labeled cRNA samples were fragmented and prepared for one-color-based hybridization (Gene Expression Hybridization Kit, Agilent Technologies) and hybridized at 65° C for 17 h on Agilent SurePrint G3 Mouse GE 8x60K Microarrays.

Article Title: Altered Gene Expression in Prefrontal Cortex of a Fabry Disease Mouse Model
Article Snippet: Samples were analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. Following cRNA clean-up and quantification, Cyanine-3-labeled cRNA samples were fragmented and prepared for one-color-based hybridization (Gene Expression Hybridization Kit, Agilent Technologies) and hybridized at 65° C for 17 h on Agilent SurePrint G3 Mouse GE 8x60K Microarrays.

Article Title: Signatures of Altered Gene Expression in Dorsal Root Ganglia of a Fabry Disease Mouse Model
Article Snippet: Samples were analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio, and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. Following cRNA clean-up and quantification, Cyanine-3-labeled cRNA samples were fragmented and prepared for one-color-based hybridization (Gene Expression Hybridization Kit, Agilent Technologies) and hybridized at 65°C for 17 h on Agilent SurePrint G3 Mouse GE 8 × 60K Microarrays.

Article Title: Signatures of Altered Gene Expression in Dorsal Root Ganglia of a Fabry Disease Mouse Model
Article Snippet: Samples were analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio, and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. Following cRNA clean-up and quantification, Cyanine-3-labeled cRNA samples were fragmented and prepared for one-color-based hybridization (Gene Expression Hybridization Kit, Agilent Technologies) and hybridized at 65°C for 17 h on Agilent SurePrint G3 Mouse GE 8 × 60K Microarrays.

Article Title: Convergent Differential Regulation of Parvalbumin in the Brains of Vocal Learners
Article Snippet: Just before cDNA amplification, a 1∶200000 dilution of Agilent One Color RNA Spike-In Mix (Agilent Technologies) was added to five nanograms of total RNA from each sample. .. The Spike-in recognizes only control non-vertebrate oligos on the array, which allows us to detect amplification and hybridization artifacts.

Expressing:

Article Title: Identifying Reliable Diagnostic/Predictive Biomarkers for Rheumatoid Arthritis
Article Snippet: Paragraph title: Gene expression profiling microarray analysis (IMGM Laboratories, Germany) ... Total RNA (100 ng) was spiked with in vitro–synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies, Santa Clara, CA, USA).

Article Title: Custom microarray construction and analysis for determining potential biomarkers of subchronic androgen exposure in the Eastern Mosquitofish (Gambusia holbrooki)
Article Snippet: In brief, T7 primers were annealed to 1000 ng RNA with the addition of RNA spike-in controls (One color RNA spike-in mix; Agilent, Santa Clara, USA) by incubation at 65°C for 10 minutes. cDNA synthesis using MMLV-RT was conducted at 40°C for 2 hours followed by 65°C for 15 minutes. cDNA was reverse-transcribed into cRNA using a T7 RNA polymerase with the addition of Cy3 to the reaction. .. In brief, T7 primers were annealed to 1000 ng RNA with the addition of RNA spike-in controls (One color RNA spike-in mix; Agilent, Santa Clara, USA) by incubation at 65°C for 10 minutes. cDNA synthesis using MMLV-RT was conducted at 40°C for 2 hours followed by 65°C for 15 minutes. cDNA was reverse-transcribed into cRNA using a T7 RNA polymerase with the addition of Cy3 to the reaction.

Article Title: Altered Gene Expression in Prefrontal Cortex of a Fabry Disease Mouse Model
Article Snippet: Paragraph title: Microarray expression profiling ... For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies).

Article Title: Signatures of Altered Gene Expression in Dorsal Root Ganglia of a Fabry Disease Mouse Model
Article Snippet: Paragraph title: Microarray expression profiling ... For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio, and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies).

Article Title: Integrated omics approaches provide strategies for rapid erythromycin yield increase in Saccharopolyspora erythraea
Article Snippet: Sample labelling and hybridization were performed at IMGM (Germany). .. 300 ng of total RNA was spiked using One-Color RNA Spike-In Mix (Agilent Technologies) and subjected to reverse transcription, subsequent in vitro transcription, Cy3 labelling using Full Spectrum™ MultiStart Primers for T7 IVT (System Biosciences) and One-Color Quick-Amp Labelling Kit (Agilent Technologies) following manufacturer’s protocols. cRNA yield and integrity were determined using NanoDrop ND-1000 (Thermo Scientific) and 2100 Bioanalyzer (Agilent Technologies). cRNA was cleaned and hybridized to microarrays using Gene Expression Hybridization Kit (Agilent Technologies) following manufacturer’s protocols. .. Hybridized microarrays were washed using Gene Expression Wash Buffers (Agilent Technologies) followed by drying with acetonitrile.

Article Title: Transcriptomics of the Interaction between the Monopartite Phloem-Limited Geminivirus Tomato Yellow Leaf Curl Sardinia Virus and Solanum lycopersicum Highlights a Role for Plant Hormones, Autophagy and Plant Immune System Fine Tuning during Infection
Article Snippet: RNA integrity was examined with the Experion Automated Electrophoresis Station (Bio-Rad). cDNA synthesis, hybridization, washing and scanning were performed by IMGM Laboratories ( www.imgm.com ) as follows: 500 ng of total RNA were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), which serve as internal labelling control for linearity, sensitivity and accuracy. .. RNA integrity was examined with the Experion Automated Electrophoresis Station (Bio-Rad). cDNA synthesis, hybridization, washing and scanning were performed by IMGM Laboratories ( www.imgm.com ) as follows: 500 ng of total RNA were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), which serve as internal labelling control for linearity, sensitivity and accuracy.

Genome Wide:

Article Title: Altered Gene Expression in Prefrontal Cortex of a Fabry Disease Mouse Model
Article Snippet: Genome-wide expression profiling was carried out by IMGM Laboratories (Munich, Germany) using Agilent SurePrint G3 Mouse GE 8x60K Microarrays in combination with a one-color based hybridization protocol. .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies).

Article Title: Altered Gene Expression in Prefrontal Cortex of a Fabry Disease Mouse Model
Article Snippet: Genome-wide expression profiling was carried out by IMGM Laboratories (Munich, Germany) using Agilent SurePrint G3 Mouse GE 8x60K Microarrays in combination with a one-color based hybridization protocol. .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies).

Article Title: Signatures of Altered Gene Expression in Dorsal Root Ganglia of a Fabry Disease Mouse Model
Article Snippet: Genome-wide expression profiling was carried out by IMGM Laboratories (Munich, Germany) using Agilent SurePrint G3 Mouse GE 8 × 60K Microarrays in combination with a one-color based hybridization protocol. .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio, and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies).

Article Title: Signatures of Altered Gene Expression in Dorsal Root Ganglia of a Fabry Disease Mouse Model
Article Snippet: Genome-wide expression profiling was carried out by IMGM Laboratories (Munich, Germany) using Agilent SurePrint G3 Mouse GE 8 × 60K Microarrays in combination with a one-color based hybridization protocol. .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio, and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies).

Hybridization:

Article Title: Identifying Reliable Diagnostic/Predictive Biomarkers for Rheumatoid Arthritis
Article Snippet: Total RNA (100 ng) was spiked with in vitro–synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies, Santa Clara, CA, USA). .. Total RNA (100 ng) was spiked with in vitro–synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies, Santa Clara, CA, USA).

Article Title: Custom microarray construction and analysis for determining potential biomarkers of subchronic androgen exposure in the Eastern Mosquitofish (Gambusia holbrooki)
Article Snippet: Paragraph title: Microarray labeling and hybridization ... In brief, T7 primers were annealed to 1000 ng RNA with the addition of RNA spike-in controls (One color RNA spike-in mix; Agilent, Santa Clara, USA) by incubation at 65°C for 10 minutes. cDNA synthesis using MMLV-RT was conducted at 40°C for 2 hours followed by 65°C for 15 minutes. cDNA was reverse-transcribed into cRNA using a T7 RNA polymerase with the addition of Cy3 to the reaction.

Article Title: Altered Gene Expression in Prefrontal Cortex of a Fabry Disease Mouse Model
Article Snippet: Genome-wide expression profiling was carried out by IMGM Laboratories (Munich, Germany) using Agilent SurePrint G3 Mouse GE 8x60K Microarrays in combination with a one-color based hybridization protocol. .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies).

Article Title: Altered Gene Expression in Prefrontal Cortex of a Fabry Disease Mouse Model
Article Snippet: Genome-wide expression profiling was carried out by IMGM Laboratories (Munich, Germany) using Agilent SurePrint G3 Mouse GE 8x60K Microarrays in combination with a one-color based hybridization protocol. .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies).

Article Title: Signatures of Altered Gene Expression in Dorsal Root Ganglia of a Fabry Disease Mouse Model
Article Snippet: Genome-wide expression profiling was carried out by IMGM Laboratories (Munich, Germany) using Agilent SurePrint G3 Mouse GE 8 × 60K Microarrays in combination with a one-color based hybridization protocol. .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio, and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies).

Article Title: Signatures of Altered Gene Expression in Dorsal Root Ganglia of a Fabry Disease Mouse Model
Article Snippet: Genome-wide expression profiling was carried out by IMGM Laboratories (Munich, Germany) using Agilent SurePrint G3 Mouse GE 8 × 60K Microarrays in combination with a one-color based hybridization protocol. .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio, and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies).

Article Title: Integrated omics approaches provide strategies for rapid erythromycin yield increase in Saccharopolyspora erythraea
Article Snippet: Sample labelling and hybridization were performed at IMGM (Germany). .. 300 ng of total RNA was spiked using One-Color RNA Spike-In Mix (Agilent Technologies) and subjected to reverse transcription, subsequent in vitro transcription, Cy3 labelling using Full Spectrum™ MultiStart Primers for T7 IVT (System Biosciences) and One-Color Quick-Amp Labelling Kit (Agilent Technologies) following manufacturer’s protocols. cRNA yield and integrity were determined using NanoDrop ND-1000 (Thermo Scientific) and 2100 Bioanalyzer (Agilent Technologies). cRNA was cleaned and hybridized to microarrays using Gene Expression Hybridization Kit (Agilent Technologies) following manufacturer’s protocols. .. Hybridized microarrays were washed using Gene Expression Wash Buffers (Agilent Technologies) followed by drying with acetonitrile.

Article Title: Transcriptomics of the Interaction between the Monopartite Phloem-Limited Geminivirus Tomato Yellow Leaf Curl Sardinia Virus and Solanum lycopersicum Highlights a Role for Plant Hormones, Autophagy and Plant Immune System Fine Tuning during Infection
Article Snippet: RNA concentration and purity were determined with the NanoDrop ND-1000 spectrophotometer (Thermo Scientific, Wilmington, USA). .. RNA integrity was examined with the Experion Automated Electrophoresis Station (Bio-Rad). cDNA synthesis, hybridization, washing and scanning were performed by IMGM Laboratories ( www.imgm.com ) as follows: 500 ng of total RNA were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), which serve as internal labelling control for linearity, sensitivity and accuracy. .. The spiked total RNA was reverse transcribed into cDNA and then converted into labelled cRNA by in vitro transcription (Quick-Amp Labeling Kit One-Color, Agilent Technologies) incorporating Cyanine-3-CTP. cRNA concentration (ng/µl), RNA absorbance ratio (260 nm/280 nm) and Cyanine-3 dye concentration (pmol/µl) were checked for all cRNA samples using the NanoDrop ND-1000 UV-VIS spectrophotometer.

Microarray:

Article Title: Identifying Reliable Diagnostic/Predictive Biomarkers for Rheumatoid Arthritis
Article Snippet: Paragraph title: Gene expression profiling microarray analysis (IMGM Laboratories, Germany) ... Total RNA (100 ng) was spiked with in vitro–synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies, Santa Clara, CA, USA).

Article Title: Custom microarray construction and analysis for determining potential biomarkers of subchronic androgen exposure in the Eastern Mosquitofish (Gambusia holbrooki)
Article Snippet: Paragraph title: Microarray labeling and hybridization ... In brief, T7 primers were annealed to 1000 ng RNA with the addition of RNA spike-in controls (One color RNA spike-in mix; Agilent, Santa Clara, USA) by incubation at 65°C for 10 minutes. cDNA synthesis using MMLV-RT was conducted at 40°C for 2 hours followed by 65°C for 15 minutes. cDNA was reverse-transcribed into cRNA using a T7 RNA polymerase with the addition of Cy3 to the reaction.

Article Title: Altered Gene Expression in Prefrontal Cortex of a Fabry Disease Mouse Model
Article Snippet: Paragraph title: Microarray expression profiling ... For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies).

Article Title: Signatures of Altered Gene Expression in Dorsal Root Ganglia of a Fabry Disease Mouse Model
Article Snippet: Paragraph title: Microarray expression profiling ... For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio, and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies).

Article Title: Integrated omics approaches provide strategies for rapid erythromycin yield increase in Saccharopolyspora erythraea
Article Snippet: 300 ng of total RNA was spiked using One-Color RNA Spike-In Mix (Agilent Technologies) and subjected to reverse transcription, subsequent in vitro transcription, Cy3 labelling using Full Spectrum™ MultiStart Primers for T7 IVT (System Biosciences) and One-Color Quick-Amp Labelling Kit (Agilent Technologies) following manufacturer’s protocols. cRNA yield and integrity were determined using NanoDrop ND-1000 (Thermo Scientific) and 2100 Bioanalyzer (Agilent Technologies). cRNA was cleaned and hybridized to microarrays using Gene Expression Hybridization Kit (Agilent Technologies) following manufacturer’s protocols. .. 300 ng of total RNA was spiked using One-Color RNA Spike-In Mix (Agilent Technologies) and subjected to reverse transcription, subsequent in vitro transcription, Cy3 labelling using Full Spectrum™ MultiStart Primers for T7 IVT (System Biosciences) and One-Color Quick-Amp Labelling Kit (Agilent Technologies) following manufacturer’s protocols. cRNA yield and integrity were determined using NanoDrop ND-1000 (Thermo Scientific) and 2100 Bioanalyzer (Agilent Technologies). cRNA was cleaned and hybridized to microarrays using Gene Expression Hybridization Kit (Agilent Technologies) following manufacturer’s protocols.

Article Title: Transcriptomics of the Interaction between the Monopartite Phloem-Limited Geminivirus Tomato Yellow Leaf Curl Sardinia Virus and Solanum lycopersicum Highlights a Role for Plant Hormones, Autophagy and Plant Immune System Fine Tuning during Infection
Article Snippet: Paragraph title: RNA extraction and microarray experiment ... RNA integrity was examined with the Experion Automated Electrophoresis Station (Bio-Rad). cDNA synthesis, hybridization, washing and scanning were performed by IMGM Laboratories ( www.imgm.com ) as follows: 500 ng of total RNA were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), which serve as internal labelling control for linearity, sensitivity and accuracy.

Blocking Assay:

Article Title: Convergent Differential Regulation of Parvalbumin in the Brains of Vocal Learners
Article Snippet: Just before cDNA amplification, a 1∶200000 dilution of Agilent One Color RNA Spike-In Mix (Agilent Technologies) was added to five nanograms of total RNA from each sample. .. Just before cDNA amplification, a 1∶200000 dilution of Agilent One Color RNA Spike-In Mix (Agilent Technologies) was added to five nanograms of total RNA from each sample.

Software:

Article Title: Altered Gene Expression in Prefrontal Cortex of a Fabry Disease Mouse Model
Article Snippet: For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. After hybridization, microarrays were washed with increasing stringency using Triton X-102 supplemented Gene Expression Wash Buffers (Agilent Technologies) followed by drying with acetonitrile (Sigma).

Article Title: Altered Gene Expression in Prefrontal Cortex of a Fabry Disease Mouse Model
Article Snippet: For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. After hybridization, microarrays were washed with increasing stringency using Triton X-102 supplemented Gene Expression Wash Buffers (Agilent Technologies) followed by drying with acetonitrile (Sigma).

Article Title: Signatures of Altered Gene Expression in Dorsal Root Ganglia of a Fabry Disease Mouse Model
Article Snippet: For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio, and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. After hybridization, microarrays were washed with increasing stringency using Triton X-102 supplemented Gene Expression Wash Buffers (Agilent Technologies) followed by drying with acetonitrile (Sigma).

Article Title: Signatures of Altered Gene Expression in Dorsal Root Ganglia of a Fabry Disease Mouse Model
Article Snippet: For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio, and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. After hybridization, microarrays were washed with increasing stringency using Triton X-102 supplemented Gene Expression Wash Buffers (Agilent Technologies) followed by drying with acetonitrile (Sigma).

Article Title: Integrated omics approaches provide strategies for rapid erythromycin yield increase in Saccharopolyspora erythraea
Article Snippet: 300 ng of total RNA was spiked using One-Color RNA Spike-In Mix (Agilent Technologies) and subjected to reverse transcription, subsequent in vitro transcription, Cy3 labelling using Full Spectrum™ MultiStart Primers for T7 IVT (System Biosciences) and One-Color Quick-Amp Labelling Kit (Agilent Technologies) following manufacturer’s protocols. cRNA yield and integrity were determined using NanoDrop ND-1000 (Thermo Scientific) and 2100 Bioanalyzer (Agilent Technologies). cRNA was cleaned and hybridized to microarrays using Gene Expression Hybridization Kit (Agilent Technologies) following manufacturer’s protocols. .. 300 ng of total RNA was spiked using One-Color RNA Spike-In Mix (Agilent Technologies) and subjected to reverse transcription, subsequent in vitro transcription, Cy3 labelling using Full Spectrum™ MultiStart Primers for T7 IVT (System Biosciences) and One-Color Quick-Amp Labelling Kit (Agilent Technologies) following manufacturer’s protocols. cRNA yield and integrity were determined using NanoDrop ND-1000 (Thermo Scientific) and 2100 Bioanalyzer (Agilent Technologies). cRNA was cleaned and hybridized to microarrays using Gene Expression Hybridization Kit (Agilent Technologies) following manufacturer’s protocols.

Article Title: Transcriptomics of the Interaction between the Monopartite Phloem-Limited Geminivirus Tomato Yellow Leaf Curl Sardinia Virus and Solanum lycopersicum Highlights a Role for Plant Hormones, Autophagy and Plant Immune System Fine Tuning during Infection
Article Snippet: RNA integrity was examined with the Experion Automated Electrophoresis Station (Bio-Rad). cDNA synthesis, hybridization, washing and scanning were performed by IMGM Laboratories ( www.imgm.com ) as follows: 500 ng of total RNA were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), which serve as internal labelling control for linearity, sensitivity and accuracy. .. Following cRNAclean-up and quantification (NanoDrop ND-1000), 1.65 µg of each Cyanine-3-labeled cRNA sample was fragmented and prepared for One-Color based hybridization (Gene Expression Hybridization Kit, Agilent Technologies). cRNA samples were hybridized at 65°C for 17 h on separate Tomato Gene Expression Microarrays 4×44K (Agilent Technology) containing 43803 probes, corresponding to about 33000 tomato genes (expressed sequences).

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    Agilent technologies positive control one color rna spike in mix
    Positive Control One Color Rna Spike In Mix, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 80/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/positive control one color rna spike in mix/product/Agilent technologies
    Average 80 stars, based on 2 article reviews
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    positive control one color rna spike in mix - by Bioz Stars, 2019-10
    80/100 stars
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