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Agilent technologies one color rna spike in mix
One Color Rna Spike In Mix, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/one color rna spike in mix/product/Agilent technologies
Average 88 stars, based on 2 article reviews
Price from $9.99 to $1999.99
one color rna spike in mix - by Bioz Stars, 2020-05
88/100 stars

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Amplification:

Article Title: Convergent Differential Regulation of Parvalbumin in the Brains of Vocal Learners
Article Snippet: .. Just before cDNA amplification, a 1∶200000 dilution of Agilent One Color RNA Spike-In Mix (Agilent Technologies) was added to five nanograms of total RNA from each sample. .. The Spike-in recognizes only control non-vertebrate oligos on the array, which allows us to detect amplification and hybridization artifacts.

In Vitro:

Article Title: Altered Gene Expression in Prefrontal Cortex of a Fabry Disease Mouse Model
Article Snippet: .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. Following cRNA clean-up and quantification, Cyanine-3-labeled cRNA samples were fragmented and prepared for one-color-based hybridization (Gene Expression Hybridization Kit, Agilent Technologies) and hybridized at 65° C for 17 h on Agilent SurePrint G3 Mouse GE 8x60K Microarrays.

Article Title: Signatures of Altered Gene Expression in Dorsal Root Ganglia of a Fabry Disease Mouse Model
Article Snippet: .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio, and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. Following cRNA clean-up and quantification, Cyanine-3-labeled cRNA samples were fragmented and prepared for one-color-based hybridization (Gene Expression Hybridization Kit, Agilent Technologies) and hybridized at 65°C for 17 h on Agilent SurePrint G3 Mouse GE 8 × 60K Microarrays.

Article Title: Altered Gene Expression in Prefrontal Cortex of a Fabry Disease Mouse Model
Article Snippet: .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. Following cRNA clean-up and quantification, Cyanine-3-labeled cRNA samples were fragmented and prepared for one-color-based hybridization (Gene Expression Hybridization Kit, Agilent Technologies) and hybridized at 65° C for 17 h on Agilent SurePrint G3 Mouse GE 8x60K Microarrays.

Article Title: Integrated omics approaches provide strategies for rapid erythromycin yield increase in Saccharopolyspora erythraea
Article Snippet: .. 300 ng of total RNA was spiked using One-Color RNA Spike-In Mix (Agilent Technologies) and subjected to reverse transcription, subsequent in vitro transcription, Cy3 labelling using Full Spectrum™ MultiStart Primers for T7 IVT (System Biosciences) and One-Color Quick-Amp Labelling Kit (Agilent Technologies) following manufacturer’s protocols. cRNA yield and integrity were determined using NanoDrop ND-1000 (Thermo Scientific) and 2100 Bioanalyzer (Agilent Technologies). cRNA was cleaned and hybridized to microarrays using Gene Expression Hybridization Kit (Agilent Technologies) following manufacturer’s protocols. .. Hybridized microarrays were washed using Gene Expression Wash Buffers (Agilent Technologies) followed by drying with acetonitrile.

Article Title: Signatures of Altered Gene Expression in Dorsal Root Ganglia of a Fabry Disease Mouse Model
Article Snippet: .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio, and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. Following cRNA clean-up and quantification, Cyanine-3-labeled cRNA samples were fragmented and prepared for one-color-based hybridization (Gene Expression Hybridization Kit, Agilent Technologies) and hybridized at 65°C for 17 h on Agilent SurePrint G3 Mouse GE 8 × 60K Microarrays.

Article Title: Transcriptomics of the Interaction between the Monopartite Phloem-Limited Geminivirus Tomato Yellow Leaf Curl Sardinia Virus and Solanum lycopersicum Highlights a Role for Plant Hormones, Autophagy and Plant Immune System Fine Tuning during Infection
Article Snippet: .. RNA integrity was examined with the Experion Automated Electrophoresis Station (Bio-Rad). cDNA synthesis, hybridization, washing and scanning were performed by IMGM Laboratories ( www.imgm.com ) as follows: 500 ng of total RNA were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), which serve as internal labelling control for linearity, sensitivity and accuracy. .. The spiked total RNA was reverse transcribed into cDNA and then converted into labelled cRNA by in vitro transcription (Quick-Amp Labeling Kit One-Color, Agilent Technologies) incorporating Cyanine-3-CTP. cRNA concentration (ng/µl), RNA absorbance ratio (260 nm/280 nm) and Cyanine-3 dye concentration (pmol/µl) were checked for all cRNA samples using the NanoDrop ND-1000 UV-VIS spectrophotometer.

Synthesized:

Article Title: Altered Gene Expression in Prefrontal Cortex of a Fabry Disease Mouse Model
Article Snippet: .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. Following cRNA clean-up and quantification, Cyanine-3-labeled cRNA samples were fragmented and prepared for one-color-based hybridization (Gene Expression Hybridization Kit, Agilent Technologies) and hybridized at 65° C for 17 h on Agilent SurePrint G3 Mouse GE 8x60K Microarrays.

Article Title: Signatures of Altered Gene Expression in Dorsal Root Ganglia of a Fabry Disease Mouse Model
Article Snippet: .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio, and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. Following cRNA clean-up and quantification, Cyanine-3-labeled cRNA samples were fragmented and prepared for one-color-based hybridization (Gene Expression Hybridization Kit, Agilent Technologies) and hybridized at 65°C for 17 h on Agilent SurePrint G3 Mouse GE 8 × 60K Microarrays.

Article Title: Altered Gene Expression in Prefrontal Cortex of a Fabry Disease Mouse Model
Article Snippet: .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. Following cRNA clean-up and quantification, Cyanine-3-labeled cRNA samples were fragmented and prepared for one-color-based hybridization (Gene Expression Hybridization Kit, Agilent Technologies) and hybridized at 65° C for 17 h on Agilent SurePrint G3 Mouse GE 8x60K Microarrays.

Article Title: Signatures of Altered Gene Expression in Dorsal Root Ganglia of a Fabry Disease Mouse Model
Article Snippet: .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio, and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. Following cRNA clean-up and quantification, Cyanine-3-labeled cRNA samples were fragmented and prepared for one-color-based hybridization (Gene Expression Hybridization Kit, Agilent Technologies) and hybridized at 65°C for 17 h on Agilent SurePrint G3 Mouse GE 8 × 60K Microarrays.

Article Title: Transcriptomics of the Interaction between the Monopartite Phloem-Limited Geminivirus Tomato Yellow Leaf Curl Sardinia Virus and Solanum lycopersicum Highlights a Role for Plant Hormones, Autophagy and Plant Immune System Fine Tuning during Infection
Article Snippet: .. RNA integrity was examined with the Experion Automated Electrophoresis Station (Bio-Rad). cDNA synthesis, hybridization, washing and scanning were performed by IMGM Laboratories ( www.imgm.com ) as follows: 500 ng of total RNA were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), which serve as internal labelling control for linearity, sensitivity and accuracy. .. The spiked total RNA was reverse transcribed into cDNA and then converted into labelled cRNA by in vitro transcription (Quick-Amp Labeling Kit One-Color, Agilent Technologies) incorporating Cyanine-3-CTP. cRNA concentration (ng/µl), RNA absorbance ratio (260 nm/280 nm) and Cyanine-3 dye concentration (pmol/µl) were checked for all cRNA samples using the NanoDrop ND-1000 UV-VIS spectrophotometer.

Labeling:

Article Title: Altered Gene Expression in Prefrontal Cortex of a Fabry Disease Mouse Model
Article Snippet: .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. Following cRNA clean-up and quantification, Cyanine-3-labeled cRNA samples were fragmented and prepared for one-color-based hybridization (Gene Expression Hybridization Kit, Agilent Technologies) and hybridized at 65° C for 17 h on Agilent SurePrint G3 Mouse GE 8x60K Microarrays.

Article Title: Signatures of Altered Gene Expression in Dorsal Root Ganglia of a Fabry Disease Mouse Model
Article Snippet: .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio, and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. Following cRNA clean-up and quantification, Cyanine-3-labeled cRNA samples were fragmented and prepared for one-color-based hybridization (Gene Expression Hybridization Kit, Agilent Technologies) and hybridized at 65°C for 17 h on Agilent SurePrint G3 Mouse GE 8 × 60K Microarrays.

Article Title: Altered Gene Expression in Prefrontal Cortex of a Fabry Disease Mouse Model
Article Snippet: .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. Following cRNA clean-up and quantification, Cyanine-3-labeled cRNA samples were fragmented and prepared for one-color-based hybridization (Gene Expression Hybridization Kit, Agilent Technologies) and hybridized at 65° C for 17 h on Agilent SurePrint G3 Mouse GE 8x60K Microarrays.

Article Title: Signatures of Altered Gene Expression in Dorsal Root Ganglia of a Fabry Disease Mouse Model
Article Snippet: .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio, and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. Following cRNA clean-up and quantification, Cyanine-3-labeled cRNA samples were fragmented and prepared for one-color-based hybridization (Gene Expression Hybridization Kit, Agilent Technologies) and hybridized at 65°C for 17 h on Agilent SurePrint G3 Mouse GE 8 × 60K Microarrays.

Electrophoresis:

Article Title: Transcriptomics of the Interaction between the Monopartite Phloem-Limited Geminivirus Tomato Yellow Leaf Curl Sardinia Virus and Solanum lycopersicum Highlights a Role for Plant Hormones, Autophagy and Plant Immune System Fine Tuning during Infection
Article Snippet: .. RNA integrity was examined with the Experion Automated Electrophoresis Station (Bio-Rad). cDNA synthesis, hybridization, washing and scanning were performed by IMGM Laboratories ( www.imgm.com ) as follows: 500 ng of total RNA were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), which serve as internal labelling control for linearity, sensitivity and accuracy. .. The spiked total RNA was reverse transcribed into cDNA and then converted into labelled cRNA by in vitro transcription (Quick-Amp Labeling Kit One-Color, Agilent Technologies) incorporating Cyanine-3-CTP. cRNA concentration (ng/µl), RNA absorbance ratio (260 nm/280 nm) and Cyanine-3 dye concentration (pmol/µl) were checked for all cRNA samples using the NanoDrop ND-1000 UV-VIS spectrophotometer.

Concentration Assay:

Article Title: Altered Gene Expression in Prefrontal Cortex of a Fabry Disease Mouse Model
Article Snippet: .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. Following cRNA clean-up and quantification, Cyanine-3-labeled cRNA samples were fragmented and prepared for one-color-based hybridization (Gene Expression Hybridization Kit, Agilent Technologies) and hybridized at 65° C for 17 h on Agilent SurePrint G3 Mouse GE 8x60K Microarrays.

Article Title: Signatures of Altered Gene Expression in Dorsal Root Ganglia of a Fabry Disease Mouse Model
Article Snippet: .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio, and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. Following cRNA clean-up and quantification, Cyanine-3-labeled cRNA samples were fragmented and prepared for one-color-based hybridization (Gene Expression Hybridization Kit, Agilent Technologies) and hybridized at 65°C for 17 h on Agilent SurePrint G3 Mouse GE 8 × 60K Microarrays.

Article Title: Altered Gene Expression in Prefrontal Cortex of a Fabry Disease Mouse Model
Article Snippet: .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. Following cRNA clean-up and quantification, Cyanine-3-labeled cRNA samples were fragmented and prepared for one-color-based hybridization (Gene Expression Hybridization Kit, Agilent Technologies) and hybridized at 65° C for 17 h on Agilent SurePrint G3 Mouse GE 8x60K Microarrays.

Article Title: Signatures of Altered Gene Expression in Dorsal Root Ganglia of a Fabry Disease Mouse Model
Article Snippet: .. For mRNA analysis, total RNA samples were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), reverse transcribed into cDNA and then converted into Cyanine-3 labeled complementary RNA (Low Input Quick-Amp Labeling Kit One-Color, Agilent Technologies) according to the manufacturer's instructions. cRNA concentration, RNA absorbance ratio, and Cyanine-3 dye concentration were recorded using a NanoDrop ND-1000 UV-VIS spectral photometer, and quality of labeled cRNA was analyzed using the RNA 6000 Nano LabChip Kit (Agilent Technologies) on a 2100 Bioanalyzer (Agilent Technologies). .. Following cRNA clean-up and quantification, Cyanine-3-labeled cRNA samples were fragmented and prepared for one-color-based hybridization (Gene Expression Hybridization Kit, Agilent Technologies) and hybridized at 65°C for 17 h on Agilent SurePrint G3 Mouse GE 8 × 60K Microarrays.

Expressing:

Article Title: Integrated omics approaches provide strategies for rapid erythromycin yield increase in Saccharopolyspora erythraea
Article Snippet: .. 300 ng of total RNA was spiked using One-Color RNA Spike-In Mix (Agilent Technologies) and subjected to reverse transcription, subsequent in vitro transcription, Cy3 labelling using Full Spectrum™ MultiStart Primers for T7 IVT (System Biosciences) and One-Color Quick-Amp Labelling Kit (Agilent Technologies) following manufacturer’s protocols. cRNA yield and integrity were determined using NanoDrop ND-1000 (Thermo Scientific) and 2100 Bioanalyzer (Agilent Technologies). cRNA was cleaned and hybridized to microarrays using Gene Expression Hybridization Kit (Agilent Technologies) following manufacturer’s protocols. .. Hybridized microarrays were washed using Gene Expression Wash Buffers (Agilent Technologies) followed by drying with acetonitrile.

Hybridization:

Article Title: Integrated omics approaches provide strategies for rapid erythromycin yield increase in Saccharopolyspora erythraea
Article Snippet: .. 300 ng of total RNA was spiked using One-Color RNA Spike-In Mix (Agilent Technologies) and subjected to reverse transcription, subsequent in vitro transcription, Cy3 labelling using Full Spectrum™ MultiStart Primers for T7 IVT (System Biosciences) and One-Color Quick-Amp Labelling Kit (Agilent Technologies) following manufacturer’s protocols. cRNA yield and integrity were determined using NanoDrop ND-1000 (Thermo Scientific) and 2100 Bioanalyzer (Agilent Technologies). cRNA was cleaned and hybridized to microarrays using Gene Expression Hybridization Kit (Agilent Technologies) following manufacturer’s protocols. .. Hybridized microarrays were washed using Gene Expression Wash Buffers (Agilent Technologies) followed by drying with acetonitrile.

Article Title: Transcriptomics of the Interaction between the Monopartite Phloem-Limited Geminivirus Tomato Yellow Leaf Curl Sardinia Virus and Solanum lycopersicum Highlights a Role for Plant Hormones, Autophagy and Plant Immune System Fine Tuning during Infection
Article Snippet: .. RNA integrity was examined with the Experion Automated Electrophoresis Station (Bio-Rad). cDNA synthesis, hybridization, washing and scanning were performed by IMGM Laboratories ( www.imgm.com ) as follows: 500 ng of total RNA were spiked with in vitro synthesized polyadenylated transcripts (One-Color RNA Spike-In Mix, Agilent Technologies), which serve as internal labelling control for linearity, sensitivity and accuracy. .. The spiked total RNA was reverse transcribed into cDNA and then converted into labelled cRNA by in vitro transcription (Quick-Amp Labeling Kit One-Color, Agilent Technologies) incorporating Cyanine-3-CTP. cRNA concentration (ng/µl), RNA absorbance ratio (260 nm/280 nm) and Cyanine-3 dye concentration (pmol/µl) were checked for all cRNA samples using the NanoDrop ND-1000 UV-VIS spectrophotometer.

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  • 88
    Agilent technologies one color rna spike in mix
    One Color Rna Spike In Mix, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/one color rna spike in mix/product/Agilent technologies
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    one color rna spike in mix - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

    88
    Agilent technologies one color spike mix
    One Color Spike Mix, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/one color spike mix/product/Agilent technologies
    Average 88 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    one color spike mix - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

    85
    Agilent technologies one color rna spike mix stock
    One Color Rna Spike Mix Stock, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/one color rna spike mix stock/product/Agilent technologies
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    one color rna spike mix stock - by Bioz Stars, 2020-05
    85/100 stars
      Buy from Supplier

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