on column dnasei digestion  (Thermo Fisher)


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    Name:
    DNase I
    Description:
    DNase I Deoxyribonuclease I digests single and double stranded DNA to oligodeoxyribonucleotides containing a 5 phosphate Ribonuclease has been reduced to non detectable levels ApplicationsDNase I is suitable for removing DNA from protein preparations nick translating DNA and generating random fragments for dideoxy sequencing NOTE for removing DNA from RNA preparations use Amplification Grade DNase I Cat No 18068 015 SourceDNase I is purified from bovine pancreas Specific activityThe specific activity of DNase I is typically in the range of 10 000 25 000 units mg
    Catalog Number:
    18047019
    Price:
    None
    Applications:
    PCR & Real-Time PCR|Reverse Transcription
    Category:
    Proteins Enzymes Peptides
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    Structured Review

    Thermo Fisher on column dnasei digestion
    DNase I Deoxyribonuclease I digests single and double stranded DNA to oligodeoxyribonucleotides containing a 5 phosphate Ribonuclease has been reduced to non detectable levels ApplicationsDNase I is suitable for removing DNA from protein preparations nick translating DNA and generating random fragments for dideoxy sequencing NOTE for removing DNA from RNA preparations use Amplification Grade DNase I Cat No 18068 015 SourceDNase I is purified from bovine pancreas Specific activityThe specific activity of DNase I is typically in the range of 10 000 25 000 units mg
    https://www.bioz.com/result/on column dnasei digestion/product/Thermo Fisher
    Average 90 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    on column dnasei digestion - by Bioz Stars, 2020-01
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: NODULE ROOT and COCHLEATA Maintain Nodule Development and Are Legume Orthologs of Arabidopsis BLADE-ON-PETIOLE Genes [W] Genes [W] [OA]
    Article Snippet: First-strand cDNA was synthesized after DNaseI treatment followed by a reverse transcription reaction using Fermentas enzymes EN0521 and K1652. .. DNA amplification for genotyping was done using Eurobio Taq polymerase, and fragments for cloning and sequencing were amplified using TaKaRa ExTaq polymerase.

    Amplification:

    Article Title: NPD1-mediated stereoselective regulation of BIRC3 expression through cREL is decisive for neural cell survival
    Article Snippet: Protein/DNA complexes were treated with Proteinase K. DNA was purified using the phenol/chloroform extraction method and amplified by PCR using the primers depicted in . .. For immunoblot analysis of the immunoprecipitated proteins, the eluted samples were treated with DNAse I (Invitrogen).

    Article Title: NODULE ROOT and COCHLEATA Maintain Nodule Development and Are Legume Orthologs of Arabidopsis BLADE-ON-PETIOLE Genes [W] Genes [W] [OA]
    Article Snippet: Paragraph title: DNA and RNA Extraction and DNA Amplification ... First-strand cDNA was synthesized after DNaseI treatment followed by a reverse transcription reaction using Fermentas enzymes EN0521 and K1652.

    Positive Control:

    Article Title: Decline of FoxP3+ Regulatory CD4 T Cells in Peripheral Blood of Children Heavily Exposed to Malaria
    Article Snippet: PHA (1μg/ml) was used as a positive control. .. Following culture of PBMCs with protein extracts, cells were treated with 100 units of DNase I (Invitrogen) in culture media for 5 minutes and then surface stained with Biolegend anti-CD3-BrilliantViolet650 (OKT3), anti-CD4-PerCP (OKT4), anti-CD25-PE-Cy7 (BC96), anti-CD127 Pacific Blue (A019D5), BD Pharmingen anti-CD8-ABC-H7 (SK1) and Live/dead aqua amine (Invitrogen cells).

    Article Title: Rap1 GTPase Activation and Barrier Enhancement in RPE Inhibits Choroidal Neovascularization In Vivo
    Article Snippet: .. DNase-treated cryosections (Fermentas-EN0521; 0.33 U/µl in PBS, 30 min) served as a positive control for TUNEL assay. .. In vitro experiments Statistical significance was determined by Student's t -test (one-tail, equal variance) using the average values ± SEM obtained from at least triplicate samples in a given experiment, or normalized pooled data from at least 3 independent experiments, unless noted in the figure legend.

    Synthesized:

    Article Title: NODULE ROOT and COCHLEATA Maintain Nodule Development and Are Legume Orthologs of Arabidopsis BLADE-ON-PETIOLE Genes [W] Genes [W] [OA]
    Article Snippet: .. First-strand cDNA was synthesized after DNaseI treatment followed by a reverse transcription reaction using Fermentas enzymes EN0521 and K1652. .. DNA amplification for genotyping was done using Eurobio Taq polymerase, and fragments for cloning and sequencing were amplified using TaKaRa ExTaq polymerase.

    Real-time Polymerase Chain Reaction:

    Article Title: Herpes Simplex Virus Dances with Amyloid Precursor Protein while Exiting the Cell
    Article Snippet: Measurements of genome to plaque-forming unit ratios The number of viral genomes was determined in parallel by quantitative PCR both with and without DNAse treatment, using the gB primers as described . .. One aliquot is treated with 2.5 µl of DNase1 (5 units: Abgene recombinant DNase 1, Thermofisher AB) and both tubes incubated at 37°C for 30 min and then at 75°C for 10 min. After incubation, 2.5 µl (25 mg total) salmon sperm DNA (Invitrogen) is added to each tube.

    Article Title: Phytochemical study and evaluation of cytotoxicity, mutagenicity, cell cycle kinetics and gene expression of Bauhinia holophylla (Bong.) Steud. in HepG2 cells in vitro
    Article Snippet: No.: EN0521), Oligo-DT12–18 (Cat. No.: 18418012); Random Primers (Cat. No.: 48190011) and SuperScript III RT enzyme (Cat. No.: 18080-044) were purchease from Invitrogen (Eugene, OR, USA). .. PureLink™ RNA Mini Kit Ambion (Cat No.: 12183020) and Platinum® SYBR® Green qPCR SuperMix UGD (Cat. No.: 11733-038) were obtained in Life Technologies (Carlsbad, CA, USA).

    Incubation:

    Article Title: SOX2 regulates common and specific stem cell features in the CNS and endoderm derived organs
    Article Snippet: After over night incubation in full medium, cells were trypsinized and resuspended in 1 mL lysis buffer, frozen for 2 min at -80°C, and then incubated on ice, rocking, for 30 min. 50μL lysate was saved as input and 60μL Flag antibody bound beads (Sigma) were incubated with the lysates for 2h at 4°C. .. To establish DNA dependency of the protein-protein interactions 100U/ml DNase I (Invitrogen) was added to the lysate for 30 min at 4°C before immunoprecipitation.

    Article Title: NPD1-mediated stereoselective regulation of BIRC3 expression through cREL is decisive for neural cell survival
    Article Snippet: Samples were incubated with protein A/G plus agarose beads for 3 h, centrifuged, washed and eluted with 1% SDS and 0.1 M NaHCO3 . .. For immunoblot analysis of the immunoprecipitated proteins, the eluted samples were treated with DNAse I (Invitrogen).

    Article Title: Multiple Functions for Mcm2-7 ATPase Motifs During Replication Initiation
    Article Snippet: The reaction mix was incubated at 25°C at 1200 rpm for 30 minutes in a Thermomixer (Eppendorf). .. Beads were washed three times with Buffer H (25 mM HEPES-KOH (pH 7.6), 1 mM EDTA, 1 mM EGTA, 5 mM MgAc, 10 % glycerol, 0.02% NP40) containing 0.3 M KGlu and DNA bound proteins were eluted from the beads using DNase I. Eluted proteins were separated by SDS-PAGE and stained with a fluorescent protein stain (Krypton, Thermo Scientific).

    Article Title: Single-Stranded DNA Uptake during Gonococcal Transformation
    Article Snippet: .. The cells were incubated with DNA at 37°C in 5% CO2 and subsequently treated with 10 U DNase I (recombinant; Fermentas) for another 15 min at 37°C. .. After washing with phosphate-buffered saline, 50 μl of this dilution was applied to clean coverslips for microscopic analysis.

    Article Title: Decline of FoxP3+ Regulatory CD4 T Cells in Peripheral Blood of Children Heavily Exposed to Malaria
    Article Snippet: CFSE-labeled PBMC were incubated in 96-well, deep-well culture plates (Nunc, Roskilde, Denmark) at 106 PBMC/ well in 1 ml for 7 days with P . falciparum schizont extract (PfSE) (W2 strain) or protein extract from uninfected RBCs (uE) at a effector to target ratio equivalent to 1:1 PBMC:infected RBC. .. Following culture of PBMCs with protein extracts, cells were treated with 100 units of DNase I (Invitrogen) in culture media for 5 minutes and then surface stained with Biolegend anti-CD3-BrilliantViolet650 (OKT3), anti-CD4-PerCP (OKT4), anti-CD25-PE-Cy7 (BC96), anti-CD127 Pacific Blue (A019D5), BD Pharmingen anti-CD8-ABC-H7 (SK1) and Live/dead aqua amine (Invitrogen cells).

    Article Title: Herpes Simplex Virus Dances with Amyloid Precursor Protein while Exiting the Cell
    Article Snippet: .. One aliquot is treated with 2.5 µl of DNase1 (5 units: Abgene recombinant DNase 1, Thermofisher AB) and both tubes incubated at 37°C for 30 min and then at 75°C for 10 min. After incubation, 2.5 µl (25 mg total) salmon sperm DNA (Invitrogen) is added to each tube. .. DNA is then extracted with Qiagen mini kit for a final volume of 200 µl.

    Article Title: A conserved MCM single-stranded DNA binding element is essential for replication initiation
    Article Snippet: The reaction mix was incubated at 25°C at 1200 rpm for 30 min in a thermomixer (Eppendorf). .. Beads were washed three times with Buffer H containing 0.3 M potassium glutamate and DNA bound proteins were eluted from the beads using DNase I. Eluted proteins were separated by SDS-PAGE and stained with a fluorescent protein stain (Krypton, Thermo Scientific).

    Article Title: Regulated specific proteolysis of the Cajal body marker protein coilin
    Article Snippet: .. GST-tagged coilin protein was purified as previously described , with minor changes: prior to separation by SDS-PAGE, protein was incubated at 37°C for 30 min with DNase I and RNase A/T1 cocktail (Invitrogen, Carlsbad, CA, USA). .. The concentrated electro-eluted coilin protein was incubated on ice for 30 min in a final concentration of 21 mM α-cyclodextrin, 259 mM NaCl and 1.34 μM coilin.

    Article Title: Coilin displays differential affinity for specific RNAs in vivo and is linked to telomerase RNA biogenesis
    Article Snippet: .. Equal concentrations of protein were incubated with or without DNase I and RNase A/T1 cocktail (Invitrogen, Carlsbad, CA, USA) at 37°C for 30 min then centrifuged at 16000 g for 5 min to visualize protein precipitation. .. Equal concentrations of untreated proteins were subjected to SDS-PAGE followed by Coomassie staining; proteins were then nuclease treated and centrifuged as described above and equal volumes of the soluble protein subjected to SDS-PAGE and Coomassie staining.

    Article Title: Rap1 GTPase Activation and Barrier Enhancement in RPE Inhibits Choroidal Neovascularization In Vivo
    Article Snippet: TUNEL Staining Eyes were fixed in 4% PFA, incubated in 15% sucrose until sinking, and then incubated in 30% sucrose overnight at 4°C. .. DNase-treated cryosections (Fermentas-EN0521; 0.33 U/µl in PBS, 30 min) served as a positive control for TUNEL assay.

    Article Title: Concerted Spatio-Temporal Dynamics of Imported DNA and ComE DNA Uptake Protein during Gonococcal Transformation
    Article Snippet: .. The cells were incubated with DNA at 37°C with 5% CO2 and subsequently treated with 10 U DNAse I (recombinant, Fermentas ) for further 15 min at 37°C. .. For each comparative experiment, the different strains or conditions were characterized on the same day using the same stock of labeled DNA.

    Article Title: Testing an Aflatoxin B1 Gene Signature in Rat Archival Tissues
    Article Snippet: RNA was eluted at 14,000g after incubation for 1 min with 30 μL of 65°C RNase-free water. .. Removal of any contaminating DNA was performed for 15 min at room temp in an off-column digestion with 15 μL (7.5 μL 10X buffer, 7.5 μL enzyme) of DNase I (RNase-free, Invitrogen) and the enzyme was inactivated according to protocol.

    Formalin-fixed Paraffin-Embedded:

    Article Title: Testing an Aflatoxin B1 Gene Signature in Rat Archival Tissues
    Article Snippet: Paragraph title: RNA Extraction from FFPE Blocks ... Removal of any contaminating DNA was performed for 15 min at room temp in an off-column digestion with 15 μL (7.5 μL 10X buffer, 7.5 μL enzyme) of DNase I (RNase-free, Invitrogen) and the enzyme was inactivated according to protocol.

    Expressing:

    Article Title: SOX2 regulates common and specific stem cell features in the CNS and endoderm derived organs
    Article Snippet: Cells were then transfected with 5μg of each expression vector using Lipofectamine 2000, according to manufacturers protocol. .. To establish DNA dependency of the protein-protein interactions 100U/ml DNase I (Invitrogen) was added to the lysate for 30 min at 4°C before immunoprecipitation.

    Modification:

    Article Title: Phytochemical study and evaluation of cytotoxicity, mutagenicity, cell cycle kinetics and gene expression of Bauhinia holophylla (Bong.) Steud. in HepG2 cells in vitro
    Article Snippet: Dulbecco’s Modified Eagle Medium (DMEM) (Cat No.: 11885084) fetal bovine serum (FBS) (Cat No.: 12657-029), 1% antibiotic–antimycotic solution (Cat No.: 15240-062) were purchased from Gibco (Grand Island, NY, USA). .. No.: EN0521), Oligo-DT12–18 (Cat. No.: 18418012); Random Primers (Cat. No.: 48190011) and SuperScript III RT enzyme (Cat. No.: 18080-044) were purchease from Invitrogen (Eugene, OR, USA).

    Western Blot:

    Article Title: NPD1-mediated stereoselective regulation of BIRC3 expression through cREL is decisive for neural cell survival
    Article Snippet: Paragraph title: ChIP aasay, immunoprecipitation and western blot ... For immunoblot analysis of the immunoprecipitated proteins, the eluted samples were treated with DNAse I (Invitrogen).

    Transformation Assay:

    Article Title: Coilin displays differential affinity for specific RNAs in vivo and is linked to telomerase RNA biogenesis
    Article Snippet: Partially purified GST-tagged coilin proteins were obtained by transformation into Escherichia coli BL21(DE3)pLysS cells, induction and purification over glutathione sepharose beads (GE Healthcare, Pittsburgh, PA, USA) as per the manufacturer’s protocol. .. Equal concentrations of protein were incubated with or without DNase I and RNase A/T1 cocktail (Invitrogen, Carlsbad, CA, USA) at 37°C for 30 min then centrifuged at 16000 g for 5 min to visualize protein precipitation.

    TUNEL Assay:

    Article Title: Rap1 GTPase Activation and Barrier Enhancement in RPE Inhibits Choroidal Neovascularization In Vivo
    Article Snippet: .. DNase-treated cryosections (Fermentas-EN0521; 0.33 U/µl in PBS, 30 min) served as a positive control for TUNEL assay. .. In vitro experiments Statistical significance was determined by Student's t -test (one-tail, equal variance) using the average values ± SEM obtained from at least triplicate samples in a given experiment, or normalized pooled data from at least 3 independent experiments, unless noted in the figure legend.

    Transfection:

    Article Title: SOX2 regulates common and specific stem cell features in the CNS and endoderm derived organs
    Article Snippet: Cells were then transfected with 5μg of each expression vector using Lipofectamine 2000, according to manufacturers protocol. .. To establish DNA dependency of the protein-protein interactions 100U/ml DNase I (Invitrogen) was added to the lysate for 30 min at 4°C before immunoprecipitation.

    Concentration Assay:

    Article Title: Single-Stranded DNA Uptake during Gonococcal Transformation
    Article Snippet: Labeled DNA fragments were added to the cell suspension to a final concentration of 1 ng/μl. .. The cells were incubated with DNA at 37°C in 5% CO2 and subsequently treated with 10 U DNase I (recombinant; Fermentas) for another 15 min at 37°C.

    Article Title: Regulated specific proteolysis of the Cajal body marker protein coilin
    Article Snippet: GST-tagged coilin protein was purified as previously described , with minor changes: prior to separation by SDS-PAGE, protein was incubated at 37°C for 30 min with DNase I and RNase A/T1 cocktail (Invitrogen, Carlsbad, CA, USA). .. The concentrated electro-eluted coilin protein was incubated on ice for 30 min in a final concentration of 21 mM α-cyclodextrin, 259 mM NaCl and 1.34 μM coilin.

    Article Title: Coilin displays differential affinity for specific RNAs in vivo and is linked to telomerase RNA biogenesis
    Article Snippet: Concentration of partially purified GST-tagged proteins was estimated using BioRad Protein Assay (Hercules, CA, USA). .. Equal concentrations of protein were incubated with or without DNase I and RNase A/T1 cocktail (Invitrogen, Carlsbad, CA, USA) at 37°C for 30 min then centrifuged at 16000 g for 5 min to visualize protein precipitation.

    Article Title: Concerted Spatio-Temporal Dynamics of Imported DNA and ComE DNA Uptake Protein during Gonococcal Transformation
    Article Snippet: Cy3- or YOYO-labeled PCR-fragments were added to the cell suspension to a final concentration of 1 ng/µl. .. The cells were incubated with DNA at 37°C with 5% CO2 and subsequently treated with 10 U DNAse I (recombinant, Fermentas ) for further 15 min at 37°C.

    Article Title: Testing an Aflatoxin B1 Gene Signature in Rat Archival Tissues
    Article Snippet: Removal of any contaminating DNA was performed for 15 min at room temp in an off-column digestion with 15 μL (7.5 μL 10X buffer, 7.5 μL enzyme) of DNase I (RNase-free, Invitrogen) and the enzyme was inactivated according to protocol. .. The concentration of RNA (30–50 μg/3 sections) was determined spectrophotometrically (Nanodrop, ThermoFisher, Waltham, MA, USA) and the size range by microcapillary separation (Bioanalyzer, Agilent).

    Protease Inhibitor:

    Article Title: NPD1-mediated stereoselective regulation of BIRC3 expression through cREL is decisive for neural cell survival
    Article Snippet: For immunoblot analysis of the immunoprecipitated proteins, the eluted samples were treated with DNAse I (Invitrogen). .. Western blot was performed from lysates obtained using RIPA buffer supplemented with a protease inhibitor cocktail (Sigma).

    Transferring:

    Article Title: NPD1-mediated stereoselective regulation of BIRC3 expression through cREL is decisive for neural cell survival
    Article Snippet: For immunoblot analysis of the immunoprecipitated proteins, the eluted samples were treated with DNAse I (Invitrogen). .. Total protein (30–50 mg) was mixed with Laemmli buffer containing DTT and loaded in Novex 4–12% precast gels and ran in an X-Cell running system at 120 V for 1.5 h. The transference was performed using the Trans Blot Turbo dry transferring system (Bio-Rad) on low fluorescent background PVDF membranes (GE Healthcare).

    Polymerase Chain Reaction:

    Article Title: NPD1-mediated stereoselective regulation of BIRC3 expression through cREL is decisive for neural cell survival
    Article Snippet: Protein/DNA complexes were treated with Proteinase K. DNA was purified using the phenol/chloroform extraction method and amplified by PCR using the primers depicted in . .. For immunoblot analysis of the immunoprecipitated proteins, the eluted samples were treated with DNAse I (Invitrogen).

    Article Title: Herpes Simplex Virus Dances with Amyloid Precursor Protein while Exiting the Cell
    Article Snippet: One aliquot is treated with 2.5 µl of DNase1 (5 units: Abgene recombinant DNase 1, Thermofisher AB) and both tubes incubated at 37°C for 30 min and then at 75°C for 10 min. After incubation, 2.5 µl (25 mg total) salmon sperm DNA (Invitrogen) is added to each tube. .. Samples are then diluted 1∶10−3 and 1∶10−4 in reaction buffer producing a DNA preparation ready for PCR quantification.

    Article Title: Concerted Spatio-Temporal Dynamics of Imported DNA and ComE DNA Uptake Protein during Gonococcal Transformation
    Article Snippet: Cy3- or YOYO-labeled PCR-fragments were added to the cell suspension to a final concentration of 1 ng/µl. .. The cells were incubated with DNA at 37°C with 5% CO2 and subsequently treated with 10 U DNAse I (recombinant, Fermentas ) for further 15 min at 37°C.

    Sonication:

    Article Title: NPD1-mediated stereoselective regulation of BIRC3 expression through cREL is decisive for neural cell survival
    Article Snippet: The pellet was washed three times with SDS lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris, pH 8.1), and DNA was fragmented by sonication and incubated in ChIP dilution buffer (16.7 mM Tris-HCl, pH 8.1, 1.2 mM EDTA, 1.1% Triton-X, 167 mM NaCl, 0.01% SDS and protease inhibitors) with cREL antibody (Bethyl, Montgomery, TX, USA) at 4 °C overnight. .. For immunoblot analysis of the immunoprecipitated proteins, the eluted samples were treated with DNAse I (Invitrogen).

    Recombinant:

    Article Title: Single-Stranded DNA Uptake during Gonococcal Transformation
    Article Snippet: .. The cells were incubated with DNA at 37°C in 5% CO2 and subsequently treated with 10 U DNase I (recombinant; Fermentas) for another 15 min at 37°C. .. After washing with phosphate-buffered saline, 50 μl of this dilution was applied to clean coverslips for microscopic analysis.

    Article Title: Herpes Simplex Virus Dances with Amyloid Precursor Protein while Exiting the Cell
    Article Snippet: .. One aliquot is treated with 2.5 µl of DNase1 (5 units: Abgene recombinant DNase 1, Thermofisher AB) and both tubes incubated at 37°C for 30 min and then at 75°C for 10 min. After incubation, 2.5 µl (25 mg total) salmon sperm DNA (Invitrogen) is added to each tube. .. DNA is then extracted with Qiagen mini kit for a final volume of 200 µl.

    Article Title: Concerted Spatio-Temporal Dynamics of Imported DNA and ComE DNA Uptake Protein during Gonococcal Transformation
    Article Snippet: .. The cells were incubated with DNA at 37°C with 5% CO2 and subsequently treated with 10 U DNAse I (recombinant, Fermentas ) for further 15 min at 37°C. .. For each comparative experiment, the different strains or conditions were characterized on the same day using the same stock of labeled DNA.

    Magnetic Cell Separation:

    Article Title: Decline of FoxP3+ Regulatory CD4 T Cells in Peripheral Blood of Children Heavily Exposed to Malaria
    Article Snippet: Mature stage parasites were magnet purified from culture using MACs purification columns. .. Following culture of PBMCs with protein extracts, cells were treated with 100 units of DNase I (Invitrogen) in culture media for 5 minutes and then surface stained with Biolegend anti-CD3-BrilliantViolet650 (OKT3), anti-CD4-PerCP (OKT4), anti-CD25-PE-Cy7 (BC96), anti-CD127 Pacific Blue (A019D5), BD Pharmingen anti-CD8-ABC-H7 (SK1) and Live/dead aqua amine (Invitrogen cells).

    MTT Assay:

    Article Title: Phytochemical study and evaluation of cytotoxicity, mutagenicity, cell cycle kinetics and gene expression of Bauhinia holophylla (Bong.) Steud. in HepG2 cells in vitro
    Article Snippet: The MTT salt [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide; CAS: 298-93-1], agarose (UltraPure™—Cat. .. No.: EN0521), Oligo-DT12–18 (Cat. No.: 18418012); Random Primers (Cat. No.: 48190011) and SuperScript III RT enzyme (Cat. No.: 18080-044) were purchease from Invitrogen (Eugene, OR, USA).

    Flow Cytometry:

    Article Title: Testing an Aflatoxin B1 Gene Signature in Rat Archival Tissues
    Article Snippet: Silica spin columns were centrifuged at 800g for 1 min, discarding the flow-through until all lysate was loaded. .. Removal of any contaminating DNA was performed for 15 min at room temp in an off-column digestion with 15 μL (7.5 μL 10X buffer, 7.5 μL enzyme) of DNase I (RNase-free, Invitrogen) and the enzyme was inactivated according to protocol.

    Labeling:

    Article Title: Single-Stranded DNA Uptake during Gonococcal Transformation
    Article Snippet: Labeled DNA fragments were added to the cell suspension to a final concentration of 1 ng/μl. .. The cells were incubated with DNA at 37°C in 5% CO2 and subsequently treated with 10 U DNase I (recombinant; Fermentas) for another 15 min at 37°C.

    Article Title: Concerted Spatio-Temporal Dynamics of Imported DNA and ComE DNA Uptake Protein during Gonococcal Transformation
    Article Snippet: The cells were incubated with DNA at 37°C with 5% CO2 and subsequently treated with 10 U DNAse I (recombinant, Fermentas ) for further 15 min at 37°C. .. For each comparative experiment, the different strains or conditions were characterized on the same day using the same stock of labeled DNA.

    Purification:

    Article Title: NPD1-mediated stereoselective regulation of BIRC3 expression through cREL is decisive for neural cell survival
    Article Snippet: Protein/DNA complexes were treated with Proteinase K. DNA was purified using the phenol/chloroform extraction method and amplified by PCR using the primers depicted in . .. For immunoblot analysis of the immunoprecipitated proteins, the eluted samples were treated with DNAse I (Invitrogen).

    Article Title: Decline of FoxP3+ Regulatory CD4 T Cells in Peripheral Blood of Children Heavily Exposed to Malaria
    Article Snippet: Purified parasites or uninfected RBCs were freeze thawed 3+ times (via snap freezing on liquid nitrogen and then transfer to 37°C water bath) to produce PfSE and uE and stored at -20°C. .. Following culture of PBMCs with protein extracts, cells were treated with 100 units of DNase I (Invitrogen) in culture media for 5 minutes and then surface stained with Biolegend anti-CD3-BrilliantViolet650 (OKT3), anti-CD4-PerCP (OKT4), anti-CD25-PE-Cy7 (BC96), anti-CD127 Pacific Blue (A019D5), BD Pharmingen anti-CD8-ABC-H7 (SK1) and Live/dead aqua amine (Invitrogen cells).

    Article Title: Regulated specific proteolysis of the Cajal body marker protein coilin
    Article Snippet: .. GST-tagged coilin protein was purified as previously described , with minor changes: prior to separation by SDS-PAGE, protein was incubated at 37°C for 30 min with DNase I and RNase A/T1 cocktail (Invitrogen, Carlsbad, CA, USA). .. The concentrated electro-eluted coilin protein was incubated on ice for 30 min in a final concentration of 21 mM α-cyclodextrin, 259 mM NaCl and 1.34 μM coilin.

    Article Title: Coilin displays differential affinity for specific RNAs in vivo and is linked to telomerase RNA biogenesis
    Article Snippet: Concentration of partially purified GST-tagged proteins was estimated using BioRad Protein Assay (Hercules, CA, USA). .. Equal concentrations of protein were incubated with or without DNase I and RNase A/T1 cocktail (Invitrogen, Carlsbad, CA, USA) at 37°C for 30 min then centrifuged at 16000 g for 5 min to visualize protein precipitation.

    Sequencing:

    Article Title: NODULE ROOT and COCHLEATA Maintain Nodule Development and Are Legume Orthologs of Arabidopsis BLADE-ON-PETIOLE Genes [W] Genes [W] [OA]
    Article Snippet: First-strand cDNA was synthesized after DNaseI treatment followed by a reverse transcription reaction using Fermentas enzymes EN0521 and K1652. .. DNA amplification for genotyping was done using Eurobio Taq polymerase, and fragments for cloning and sequencing were amplified using TaKaRa ExTaq polymerase.

    Lysis:

    Article Title: SOX2 regulates common and specific stem cell features in the CNS and endoderm derived organs
    Article Snippet: After over night incubation in full medium, cells were trypsinized and resuspended in 1 mL lysis buffer, frozen for 2 min at -80°C, and then incubated on ice, rocking, for 30 min. 50μL lysate was saved as input and 60μL Flag antibody bound beads (Sigma) were incubated with the lysates for 2h at 4°C. .. To establish DNA dependency of the protein-protein interactions 100U/ml DNase I (Invitrogen) was added to the lysate for 30 min at 4°C before immunoprecipitation.

    Article Title: NPD1-mediated stereoselective regulation of BIRC3 expression through cREL is decisive for neural cell survival
    Article Snippet: The pellet was washed three times with SDS lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris, pH 8.1), and DNA was fragmented by sonication and incubated in ChIP dilution buffer (16.7 mM Tris-HCl, pH 8.1, 1.2 mM EDTA, 1.1% Triton-X, 167 mM NaCl, 0.01% SDS and protease inhibitors) with cREL antibody (Bethyl, Montgomery, TX, USA) at 4 °C overnight. .. For immunoblot analysis of the immunoprecipitated proteins, the eluted samples were treated with DNAse I (Invitrogen).

    Chromatin Immunoprecipitation:

    Article Title: NPD1-mediated stereoselective regulation of BIRC3 expression through cREL is decisive for neural cell survival
    Article Snippet: Paragraph title: ChIP aasay, immunoprecipitation and western blot ... For immunoblot analysis of the immunoprecipitated proteins, the eluted samples were treated with DNAse I (Invitrogen).

    SDS Page:

    Article Title: Multiple Functions for Mcm2-7 ATPase Motifs During Replication Initiation
    Article Snippet: .. Beads were washed three times with Buffer H (25 mM HEPES-KOH (pH 7.6), 1 mM EDTA, 1 mM EGTA, 5 mM MgAc, 10 % glycerol, 0.02% NP40) containing 0.3 M KGlu and DNA bound proteins were eluted from the beads using DNase I. Eluted proteins were separated by SDS-PAGE and stained with a fluorescent protein stain (Krypton, Thermo Scientific). ..

    Article Title: A conserved MCM single-stranded DNA binding element is essential for replication initiation
    Article Snippet: .. Beads were washed three times with Buffer H containing 0.3 M potassium glutamate and DNA bound proteins were eluted from the beads using DNase I. Eluted proteins were separated by SDS-PAGE and stained with a fluorescent protein stain (Krypton, Thermo Scientific). ..

    Article Title: Regulated specific proteolysis of the Cajal body marker protein coilin
    Article Snippet: .. GST-tagged coilin protein was purified as previously described , with minor changes: prior to separation by SDS-PAGE, protein was incubated at 37°C for 30 min with DNase I and RNase A/T1 cocktail (Invitrogen, Carlsbad, CA, USA). .. The concentrated electro-eluted coilin protein was incubated on ice for 30 min in a final concentration of 21 mM α-cyclodextrin, 259 mM NaCl and 1.34 μM coilin.

    Article Title: Coilin displays differential affinity for specific RNAs in vivo and is linked to telomerase RNA biogenesis
    Article Snippet: Equal concentrations of protein were incubated with or without DNase I and RNase A/T1 cocktail (Invitrogen, Carlsbad, CA, USA) at 37°C for 30 min then centrifuged at 16000 g for 5 min to visualize protein precipitation. .. Equal concentrations of untreated proteins were subjected to SDS-PAGE followed by Coomassie staining; proteins were then nuclease treated and centrifuged as described above and equal volumes of the soluble protein subjected to SDS-PAGE and Coomassie staining.

    Plasmid Preparation:

    Article Title: SOX2 regulates common and specific stem cell features in the CNS and endoderm derived organs
    Article Snippet: Cells were then transfected with 5μg of each expression vector using Lipofectamine 2000, according to manufacturers protocol. .. To establish DNA dependency of the protein-protein interactions 100U/ml DNase I (Invitrogen) was added to the lysate for 30 min at 4°C before immunoprecipitation.

    In Vitro:

    Article Title: Decline of FoxP3+ Regulatory CD4 T Cells in Peripheral Blood of Children Heavily Exposed to Malaria
    Article Snippet: Paragraph title: Induction of Regulatory T cells by P. falciparum in vitro ... Following culture of PBMCs with protein extracts, cells were treated with 100 units of DNase I (Invitrogen) in culture media for 5 minutes and then surface stained with Biolegend anti-CD3-BrilliantViolet650 (OKT3), anti-CD4-PerCP (OKT4), anti-CD25-PE-Cy7 (BC96), anti-CD127 Pacific Blue (A019D5), BD Pharmingen anti-CD8-ABC-H7 (SK1) and Live/dead aqua amine (Invitrogen cells).

    SYBR Green Assay:

    Article Title: Phytochemical study and evaluation of cytotoxicity, mutagenicity, cell cycle kinetics and gene expression of Bauhinia holophylla (Bong.) Steud. in HepG2 cells in vitro
    Article Snippet: Sodium bicarbonate (CAS: 144-55-8), Dimethylsulfoxide (DMSO; CAS: 67-68-5), benzo[ a ]pyrene (B[ a ]P; CAS: 50-32-8), Cytochalasin B (CAS: 14930-96-2), acridine orange (AO; CAS: 10127-02-3), ethidium bromide (EB; CAS: 1239-45-8), trypan blue (CAS: 72-57-1), ethanol (EtOH; CAS: 64-17-5), methanol (MeOH; CAS: 67-56-1), RNAse solution (Cat. No.: 10109142001), propidium iodide (PI; CAS: 25535-16-4) and KiCqStart® SYBR Green Primers (CAS: KSPQ 12012) were obtained from Sigma Chemical Co. (St. Louis, MO, USA). .. No.: EN0521), Oligo-DT12–18 (Cat. No.: 18418012); Random Primers (Cat. No.: 48190011) and SuperScript III RT enzyme (Cat. No.: 18080-044) were purchease from Invitrogen (Eugene, OR, USA).

    RNA Extraction:

    Article Title: NODULE ROOT and COCHLEATA Maintain Nodule Development and Are Legume Orthologs of Arabidopsis BLADE-ON-PETIOLE Genes [W] Genes [W] [OA]
    Article Snippet: Paragraph title: DNA and RNA Extraction and DNA Amplification ... First-strand cDNA was synthesized after DNaseI treatment followed by a reverse transcription reaction using Fermentas enzymes EN0521 and K1652.

    Article Title: Testing an Aflatoxin B1 Gene Signature in Rat Archival Tissues
    Article Snippet: Paragraph title: RNA Extraction from FFPE Blocks ... Removal of any contaminating DNA was performed for 15 min at room temp in an off-column digestion with 15 μL (7.5 μL 10X buffer, 7.5 μL enzyme) of DNase I (RNase-free, Invitrogen) and the enzyme was inactivated according to protocol.

    Binding Assay:

    Article Title: Testing an Aflatoxin B1 Gene Signature in Rat Archival Tissues
    Article Snippet: In a clean tube, a volume of 600 μL binding buffer was added to 575 μL of solubilized tissue sample, then mixed and divided into two tubes (~580 μL/tube). .. Removal of any contaminating DNA was performed for 15 min at room temp in an off-column digestion with 15 μL (7.5 μL 10X buffer, 7.5 μL enzyme) of DNase I (RNase-free, Invitrogen) and the enzyme was inactivated according to protocol.

    Agarose Gel Electrophoresis:

    Article Title: Coilin displays differential affinity for specific RNAs in vivo and is linked to telomerase RNA biogenesis
    Article Snippet: Equal concentrations of protein were incubated with or without DNase I and RNase A/T1 cocktail (Invitrogen, Carlsbad, CA, USA) at 37°C for 30 min then centrifuged at 16000 g for 5 min to visualize protein precipitation. .. Equal protein concentrations were loaded into a 1% agarose gel containing ethidium bromide to visualize co-purified nucleic acid.

    In Situ:

    Article Title: Rap1 GTPase Activation and Barrier Enhancement in RPE Inhibits Choroidal Neovascularization In Vivo
    Article Snippet: Apoptotic cells were visualized by TUNEL using the In Situ Cell Death Kit-Fluorescein (Roche Diagnostics, Germany) according to manufacturer's protocol. .. DNase-treated cryosections (Fermentas-EN0521; 0.33 U/µl in PBS, 30 min) served as a positive control for TUNEL assay.

    Immunoprecipitation:

    Article Title: SOX2 regulates common and specific stem cell features in the CNS and endoderm derived organs
    Article Snippet: .. To establish DNA dependency of the protein-protein interactions 100U/ml DNase I (Invitrogen) was added to the lysate for 30 min at 4°C before immunoprecipitation. .. SOX2 binding sites are shared more within germ layers than between them. ( A ) Percentage of iI67+ cells that are SOX2+ in the E11.5 cortex, spinal cord, stomach and lung/esophagus. ( B ) FACS plots of SOX2+ cells (encircled) sorted from dissected E11.5 SOX2-GFP cortices, spinal cords, stomachs and lung/esophagus. ( C ) Seqminer heat maps showing raw reads from all SOX2 ChIP-seqs (singlets or merged replicates) within peaks called from cortex, spinal cord, stomach or lung/esophagus SOX2 ChIP-seqs and the corresponding percentage of peaks bound in all tissues based on clustering. ( D ) The average proportion of SOX2 ChIP-seq reads derived from cortex, spinal cord, stomach and lung/esophagus experiments within the different SOX2 peak sets. (TIF) (3.0M, tif)

    Article Title: NPD1-mediated stereoselective regulation of BIRC3 expression through cREL is decisive for neural cell survival
    Article Snippet: .. For immunoblot analysis of the immunoprecipitated proteins, the eluted samples were treated with DNAse I (Invitrogen). .. Western blot was performed from lysates obtained using RIPA buffer supplemented with a protease inhibitor cocktail (Sigma).

    Staining:

    Article Title: Multiple Functions for Mcm2-7 ATPase Motifs During Replication Initiation
    Article Snippet: .. Beads were washed three times with Buffer H (25 mM HEPES-KOH (pH 7.6), 1 mM EDTA, 1 mM EGTA, 5 mM MgAc, 10 % glycerol, 0.02% NP40) containing 0.3 M KGlu and DNA bound proteins were eluted from the beads using DNase I. Eluted proteins were separated by SDS-PAGE and stained with a fluorescent protein stain (Krypton, Thermo Scientific). ..

    Article Title: Decline of FoxP3+ Regulatory CD4 T Cells in Peripheral Blood of Children Heavily Exposed to Malaria
    Article Snippet: .. Following culture of PBMCs with protein extracts, cells were treated with 100 units of DNase I (Invitrogen) in culture media for 5 minutes and then surface stained with Biolegend anti-CD3-BrilliantViolet650 (OKT3), anti-CD4-PerCP (OKT4), anti-CD25-PE-Cy7 (BC96), anti-CD127 Pacific Blue (A019D5), BD Pharmingen anti-CD8-ABC-H7 (SK1) and Live/dead aqua amine (Invitrogen cells). .. Following surface staining, cells were fixed and permeabilized with eBioscience FoxP3 staining set and stained with FoxP3-PE (PCH101).

    Article Title: A conserved MCM single-stranded DNA binding element is essential for replication initiation
    Article Snippet: .. Beads were washed three times with Buffer H containing 0.3 M potassium glutamate and DNA bound proteins were eluted from the beads using DNase I. Eluted proteins were separated by SDS-PAGE and stained with a fluorescent protein stain (Krypton, Thermo Scientific). ..

    Article Title: Coilin displays differential affinity for specific RNAs in vivo and is linked to telomerase RNA biogenesis
    Article Snippet: Equal concentrations of protein were incubated with or without DNase I and RNase A/T1 cocktail (Invitrogen, Carlsbad, CA, USA) at 37°C for 30 min then centrifuged at 16000 g for 5 min to visualize protein precipitation. .. Equal concentrations of untreated proteins were subjected to SDS-PAGE followed by Coomassie staining; proteins were then nuclease treated and centrifuged as described above and equal volumes of the soluble protein subjected to SDS-PAGE and Coomassie staining.

    Article Title: Rap1 GTPase Activation and Barrier Enhancement in RPE Inhibits Choroidal Neovascularization In Vivo
    Article Snippet: Paragraph title: TUNEL Staining ... DNase-treated cryosections (Fermentas-EN0521; 0.33 U/µl in PBS, 30 min) served as a positive control for TUNEL assay.

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