on column dnasei digestion  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    DNase I
    Description:
    DNase I Deoxyribonuclease I digests single and double stranded DNA to oligodeoxyribonucleotides containing a 5 phosphate Ribonuclease has been reduced to non detectable levels ApplicationsDNase I is suitable for removing DNA from protein preparations nick translating DNA and generating random fragments for dideoxy sequencing NOTE for removing DNA from RNA preparations use Amplification Grade DNase I Cat No 18068 015 SourceDNase I is purified from bovine pancreas Specific activityThe specific activity of DNase I is typically in the range of 10 000 25 000 units mg
    Catalog Number:
    18047019
    Price:
    None
    Applications:
    PCR & Real-Time PCR|Reverse Transcription
    Category:
    Proteins Enzymes Peptides
    Buy from Supplier


    Structured Review

    Thermo Fisher on column dnasei digestion
    DNase I Deoxyribonuclease I digests single and double stranded DNA to oligodeoxyribonucleotides containing a 5 phosphate Ribonuclease has been reduced to non detectable levels ApplicationsDNase I is suitable for removing DNA from protein preparations nick translating DNA and generating random fragments for dideoxy sequencing NOTE for removing DNA from RNA preparations use Amplification Grade DNase I Cat No 18068 015 SourceDNase I is purified from bovine pancreas Specific activityThe specific activity of DNase I is typically in the range of 10 000 25 000 units mg
    https://www.bioz.com/result/on column dnasei digestion/product/Thermo Fisher
    Average 99 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    on column dnasei digestion - by Bioz Stars, 2020-09
    99/100 stars

    Images

    Related Articles

    Amplification:

    Article Title: Non-coding murine centromeric transcripts associate with and potentiate Aurora B kinase
    Article Snippet: .. Genomic DNA was removed by digestion with 2 U of DNaseI (Ambion). cDNA were synthesized from an equivalent of 500 000 cells ( A) or 100 000 cells ( B and C), using random hexamers and Superscript II reverse transcriptase (Invitrogen), and amplified by PCR. ..

    Positive Control:

    Article Title: Respiratory Syncytial Virus Infection of Human Alveolar Epithelial Cells Enhances Interferon Regulatory Factor 1 and Interleukin-1?-Converting Enzyme Gene Expression but Does Not Cause Apoptosis
    Article Snippet: .. For positive control of DNA fragmentation, A549 cells treated with DNase I (GIBCO BRL) (10 μg/ml for 10 min) were employed ( ). ..

    Synthesized:

    Article Title: Non-coding murine centromeric transcripts associate with and potentiate Aurora B kinase
    Article Snippet: .. Genomic DNA was removed by digestion with 2 U of DNaseI (Ambion). cDNA were synthesized from an equivalent of 500 000 cells ( A) or 100 000 cells ( B and C), using random hexamers and Superscript II reverse transcriptase (Invitrogen), and amplified by PCR. ..

    Isolation:

    Article Title: Human cytomegalovirus microRNAs are carried by virions and dense bodies and are delivered to target cells
    Article Snippet: .. RNA was isolated with the miRNeasy mini kit (Qiagen), including the DNase-I treatment or isolated with Trizol-LS reagent (Life Technologies). .. The DNA and RNA were also isolated from infected cells at 5 days p.i. (m.o.i.

    Cell Culture:

    Article Title: Phosphoproteome Profiling of SH-SY5y Neuroblastoma Cells Treated with Anesthetics: Sevoflurane and Isoflurane Affect the Phosphorylation of Proteins Involved in Cytoskeletal Regulation
    Article Snippet: .. Fluorescent dye staining Cells were cultured on coverslips for F-actin and G-actin staining with phalloidin-Alexa568 and DNAse I-Alexa488 (Molecular Probes, Eugene, OR), respectively. ..

    Concentration Assay:

    Article Title: Neutrophil Extracellular Traps Exhibit Antibacterial Activity against Burkholderia pseudomallei and Are Influenced by Bacterial and Host Factors
    Article Snippet: .. PMNs from healthy subjects were incubated at a concentration of 2.5 × 106 cells/ml with medium control or B. pseudomallei WT K96243 at an MOI of 10 (unless specified elsewhere) at 37°C for 90 min in the absence or presence of 100 units/ml of DNase I (Invitrogen). .. Samples were fixed with 4% (vol/vol) paraformaldehyde and blocked with 3% (wt/vol) bovine serum albumin (BSA; Sigma) in PBS for 30 min at room temperature.

    Incubation:

    Article Title: Stable Complexes Formed by HIV-1 Reverse Transcriptase at Distinct Positions on the Primer-Template Controlled by Binding Deoxynucleoside Triphosphates or Foscarnet
    Article Snippet: .. After incubation on ice for 5 min, 0.03 U DNase I (USB corp.), in RB buffer, was added and the samples were incubated at room temperature for 3 min. ..

    Article Title: Neutrophil Extracellular Traps Exhibit Antibacterial Activity against Burkholderia pseudomallei and Are Influenced by Bacterial and Host Factors
    Article Snippet: .. PMNs from healthy subjects were incubated at a concentration of 2.5 × 106 cells/ml with medium control or B. pseudomallei WT K96243 at an MOI of 10 (unless specified elsewhere) at 37°C for 90 min in the absence or presence of 100 units/ml of DNase I (Invitrogen). .. Samples were fixed with 4% (vol/vol) paraformaldehyde and blocked with 3% (wt/vol) bovine serum albumin (BSA; Sigma) in PBS for 30 min at room temperature.

    Polymerase Chain Reaction:

    Article Title: Complete, gene-specific siRNA libraries: Production and expression in mammalian cells
    Article Snippet: .. PCR-amplified cDNA encoding DsRed was subjected to partial digestion with DNase I in a buffer containing 1 mM MnCl2 , 50 mM Tris-HCl (pH 7.5), 0.5 μg/μL BSA, and 0.1–0.3 U/μg DNase I (Ambion) at 20°C for 1–10 min to generate small, blunt-ended DNA fragments (Fig. 2A ). .. Under these conditions DNase I displays little sequence specificity, cleaving all regions of the DNA (except the terminal nucleotides) at an equal rate ( ).

    Article Title: Non-coding murine centromeric transcripts associate with and potentiate Aurora B kinase
    Article Snippet: .. Genomic DNA was removed by digestion with 2 U of DNaseI (Ambion). cDNA were synthesized from an equivalent of 500 000 cells ( A) or 100 000 cells ( B and C), using random hexamers and Superscript II reverse transcriptase (Invitrogen), and amplified by PCR. ..

    Staining:

    Article Title: Phosphoproteome Profiling of SH-SY5y Neuroblastoma Cells Treated with Anesthetics: Sevoflurane and Isoflurane Affect the Phosphorylation of Proteins Involved in Cytoskeletal Regulation
    Article Snippet: .. Fluorescent dye staining Cells were cultured on coverslips for F-actin and G-actin staining with phalloidin-Alexa568 and DNAse I-Alexa488 (Molecular Probes, Eugene, OR), respectively. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Thermo Fisher on column dnase digestion
    On Column Dnase Digestion, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/on column dnase digestion/product/Thermo Fisher
    Average 92 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    on column dnase digestion - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    Image Search Results