oligo dt beads  (Qiagen)


Bioz Verified Symbol Qiagen is a verified supplier
Bioz Manufacturer Symbol Qiagen manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Qiagen oligo dt beads
    Oligo Dt Beads, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oligo dt beads/product/Qiagen
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    oligo dt beads - by Bioz Stars, 2020-02
    90/100 stars

    Images

    Related Articles

    Agarose Gel Electrophoresis:

    Article Title: Comparative Transcriptomic Analysis Reveals Candidate Genes and Pathways Involved in Larval Settlement of the Barnacle Megabalanus volcano
    Article Snippet: The quality and quantity of RNA were verified by RNAase-free agarose gel and Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). .. The mRNA was isolated with oligo-dT beads (Qiagen, Hilden, Germany), and then sequencing libraries were constructed using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, Ipswich, MA, USA) according to manufacturer’s instructions.

    Article Title: Expression profile analysis of 5-day-old neonatal piglets infected with porcine Deltacoronavirus
    Article Snippet: The RNA quality was verified using a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA) and also checked by RNase free agarose gel electrophoresis. .. Next, Poly (A) mRNA was isolated using oligo-dT beads (Qiagen).

    Article Title: Comparative transcriptome analysis of two selenium-accumulating genotypes of Aegilops tauschii Coss. in response to selenium
    Article Snippet: The quality of the purified RNA was verified by agarose gel electrophoresis, and its concentration was measured on a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) at 260 nm and 280 nm. .. Poly (A) mRNA was isolated using oligo-dT beads (Qiagen) and broken into short fragments (200 nt) with fragmentation buffer.

    Article Title: Gene expression dynamics during the gonocyte to spermatogonia transition and spermatogenesis in the domestic yak
    Article Snippet: RNA quality and concentration were measured by using Agilent 2100 Bio-analyzer (Agilent Technologies, Santa Clara, CA) and by RNase free agarose gel electrophoresis after removing the genomic DNA. .. Next, Poly (A) mRNA was isolated using oligo-dT beads (Qiagen).

    Article Title: Transcriptome Analysis of Larval Segment Formation and Secondary Loss in the Echiuran Worm Urechis unicinctus
    Article Snippet: The concentration, purity, and integrity of the RNAs were assessed using NanoDrop 2000 (Thermo Scientific, Wilmington, DE, USA), and agarose gel electrophoresis were performed to assess the quality of total RNAs. .. The mRNA was isolated using oligo-dT beads (Qiagen, Hilden, Germany) and then broken into short fragments by adding fragmentation buffer.

    RNA Extraction:

    Article Title: Comparative Transcriptomic Analysis Reveals Candidate Genes and Pathways Involved in Larval Settlement of the Barnacle Megabalanus volcano
    Article Snippet: Paragraph title: 4.3. RNA Extraction, RNA-Seq Library Construction and Sequencing ... The mRNA was isolated with oligo-dT beads (Qiagen, Hilden, Germany), and then sequencing libraries were constructed using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, Ipswich, MA, USA) according to manufacturer’s instructions.

    Article Title: Expression profile analysis of 5-day-old neonatal piglets infected with porcine Deltacoronavirus
    Article Snippet: Paragraph title: RNA extraction, library construction and sequencing ... Next, Poly (A) mRNA was isolated using oligo-dT beads (Qiagen).

    Article Title: Genome-wide transcriptome analysis reveals the molecular mechanism of high temperature-induced floral abortion in Litchi chinensis
    Article Snippet: Paragraph title: RNA extraction, library construction, and RNA-sequencing ... Oligo-dT beads (Qiagen, Valencia, USA) was used to enrich mRNA.

    Article Title: Comparative transcriptome analysis of two selenium-accumulating genotypes of Aegilops tauschii Coss. in response to selenium
    Article Snippet: Paragraph title: RNA extraction and RNA-Seq library construction and sequencing ... Poly (A) mRNA was isolated using oligo-dT beads (Qiagen) and broken into short fragments (200 nt) with fragmentation buffer.

    Article Title: Transcriptome profiling in the spathe of Anthurium andraeanum ‘Albama’ and its anthocyanin-loss mutant ‘Xueyu’
    Article Snippet: Total RNA extraction was performed using TRIzol (Promega, USA) and DNase I (Takara Bio, Japan). .. Using a Poly(A)PuristTM Kit (Ambion, now Life Technologies) and oligo-dT beads (Qiagen), the mRNA was isolated.

    Article Title: Gene expression dynamics during the gonocyte to spermatogonia transition and spermatogenesis in the domestic yak
    Article Snippet: Paragraph title: RNA extraction, library construction and sequencing ... Next, Poly (A) mRNA was isolated using oligo-dT beads (Qiagen).

    Article Title: Hepatic transcriptome analysis and identification of differentially expressed genes response to dietary oxidized fish oil in loach Misgurnus anguillicaudatus
    Article Snippet: Paragraph title: RNA extraction, cDNA library and illumina sequencing ... Poly (A) mRNA was separated using oligo-dT beads (Qiagen, Dusseldorf, Germany).

    Article Title: Transcriptomic Profiles of Brain Provide Insights into Molecular Mechanism of Feed Conversion Efficiency in Crucian Carp (Carassius auratus)
    Article Snippet: Paragraph title: 4.2. RNA Extraction, Library Preparation and Transcriptome Sequencing ... Oligo-dT beads (Qiagen, Dusseldorf, Germany) were used to separate poly (A) mRNA from the total RNA of each sample, and the fragmentation buffer was added to split all mRNA into short fragments.

    Article Title: Transcriptome Analysis of Larval Segment Formation and Secondary Loss in the Echiuran Worm Urechis unicinctus
    Article Snippet: Paragraph title: 4.3. RNA Extraction, RNA-Seq Library Construction, and Sequencing ... The mRNA was isolated using oligo-dT beads (Qiagen, Hilden, Germany) and then broken into short fragments by adding fragmentation buffer.

    Amplification:

    Article Title: Comparative transcriptome analysis of two selenium-accumulating genotypes of Aegilops tauschii Coss. in response to selenium
    Article Snippet: Poly (A) mRNA was isolated using oligo-dT beads (Qiagen) and broken into short fragments (200 nt) with fragmentation buffer. .. The generated cDNA fragments were purified using a QIAquick PCR gel extraction kit (Qiagen, Germany), washed with EB, subjected to end reparation and poly (A) addition, and ligated to sequencing adapters. cDNA fragments of interest (200 ± 25 bp) were purified and enriched by PCR amplification to complete preparation of the entire cDNA library.

    Article Title: Transcriptomic Profiles of Brain Provide Insights into Molecular Mechanism of Feed Conversion Efficiency in Crucian Carp (Carassius auratus)
    Article Snippet: Oligo-dT beads (Qiagen, Dusseldorf, Germany) were used to separate poly (A) mRNA from the total RNA of each sample, and the fragmentation buffer was added to split all mRNA into short fragments. .. Fragments with suitable size for sequencing were isolated from the agarose gels, and PCR amplification was used to enrich these fragments to construct the final cDNA libraries.

    RNA Sequencing Assay:

    Article Title: De novo Transcriptome Assembly of Floral Buds of Pineapple and Identification of Differentially Expressed Genes in Response to Ethephon Induction
    Article Snippet: Paragraph title: RNA-seq library construction and sequencing ... Poly (A) mRNA was isolated using oligo-dT beads (Qiagen).

    Article Title: Comparative Transcriptomic Analysis Reveals Candidate Genes and Pathways Involved in Larval Settlement of the Barnacle Megabalanus volcano
    Article Snippet: Paragraph title: 4.3. RNA Extraction, RNA-Seq Library Construction and Sequencing ... The mRNA was isolated with oligo-dT beads (Qiagen, Hilden, Germany), and then sequencing libraries were constructed using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, Ipswich, MA, USA) according to manufacturer’s instructions.

    Article Title: Transcriptome Profile Analysis on Ovarian Tissues of Autotetraploid Fish and Diploid Red Crucian Carp
    Article Snippet: Paragraph title: RNA Sequencing Library Construction and Illumina Sequencing ... Poly (A) was separated using oligo-dT beads (Qiagen, Dusseldorf, Germany).

    Article Title: Genome-wide transcriptome analysis reveals the molecular mechanism of high temperature-induced floral abortion in Litchi chinensis
    Article Snippet: Paragraph title: RNA extraction, library construction, and RNA-sequencing ... Oligo-dT beads (Qiagen, Valencia, USA) was used to enrich mRNA.

    Article Title: Comparative transcriptome analysis of two selenium-accumulating genotypes of Aegilops tauschii Coss. in response to selenium
    Article Snippet: Paragraph title: RNA extraction and RNA-Seq library construction and sequencing ... Poly (A) mRNA was isolated using oligo-dT beads (Qiagen) and broken into short fragments (200 nt) with fragmentation buffer.

    Article Title: Transcriptomics reveals multiple resistance mechanisms against cotton leaf curl disease in a naturally immune cotton species, Gossypium arboreum
    Article Snippet: Paragraph title: Library construction and RNA sequencing ... Poly (A) mRNA was isolated using oligo-dT beads (Qiagen, Hilden, Germany).

    Article Title: Transcriptome Analysis of Larval Segment Formation and Secondary Loss in the Echiuran Worm Urechis unicinctus
    Article Snippet: Paragraph title: 4.3. RNA Extraction, RNA-Seq Library Construction, and Sequencing ... The mRNA was isolated using oligo-dT beads (Qiagen, Hilden, Germany) and then broken into short fragments by adding fragmentation buffer.

    Synthesized:

    Article Title: De novo Transcriptome Assembly of Floral Buds of Pineapple and Identification of Differentially Expressed Genes in Response to Ethephon Induction
    Article Snippet: Poly (A) mRNA was isolated using oligo-dT beads (Qiagen). .. First-strand cDNA was synthesized by random hexamer-primed reverse transcription, and then second-strand cDNA was generated using RNase H and DNA polymerase I.

    Article Title: Genome-wide transcriptome analysis reveals the molecular mechanism of high temperature-induced floral abortion in Litchi chinensis
    Article Snippet: Oligo-dT beads (Qiagen, Valencia, USA) was used to enrich mRNA. .. Second-strand cDNA was synthesized using DNA polymerase I, RNase H, dNTPs.

    Article Title: Comparative transcriptome analysis of two selenium-accumulating genotypes of Aegilops tauschii Coss. in response to selenium
    Article Snippet: Poly (A) mRNA was isolated using oligo-dT beads (Qiagen) and broken into short fragments (200 nt) with fragmentation buffer. .. First-strand cDNA was generated by random hexamer-primed reverse transcription using the fragmented mRNA as a template, with second-strand cDNA then synthesized after addition of buffer, dNTPs, RNase H, and DNA polymerase I.

    Article Title: Transcriptomics reveals multiple resistance mechanisms against cotton leaf curl disease in a naturally immune cotton species, Gossypium arboreum
    Article Snippet: Poly (A) mRNA was isolated using oligo-dT beads (Qiagen, Hilden, Germany). .. First-strand cDNA was synthesized using random hexamer-primed reverse transcription.

    Article Title: Transcriptomic Profiles of Brain Provide Insights into Molecular Mechanism of Feed Conversion Efficiency in Crucian Carp (Carassius auratus)
    Article Snippet: Oligo-dT beads (Qiagen, Dusseldorf, Germany) were used to separate poly (A) mRNA from the total RNA of each sample, and the fragmentation buffer was added to split all mRNA into short fragments. .. Then the first-strand cDNA was synthesized using the random hexamer-primed reverse transcription, and the second-strand cDNA was generated using RNase and DNA polymerase I.

    Article Title: Transcriptome Analysis of Larval Segment Formation and Secondary Loss in the Echiuran Worm Urechis unicinctus
    Article Snippet: The mRNA was isolated using oligo-dT beads (Qiagen, Hilden, Germany) and then broken into short fragments by adding fragmentation buffer. .. The second-strand cDNA was synthesized using dNTPs, RNase H, and DNA polymerase I.

    Isolation:

    Article Title: De novo Transcriptome Assembly of Floral Buds of Pineapple and Identification of Differentially Expressed Genes in Response to Ethephon Induction
    Article Snippet: .. Poly (A) mRNA was isolated using oligo-dT beads (Qiagen). ..

    Article Title: Comparative Transcriptomic Analysis Reveals Candidate Genes and Pathways Involved in Larval Settlement of the Barnacle Megabalanus volcano
    Article Snippet: .. The mRNA was isolated with oligo-dT beads (Qiagen, Hilden, Germany), and then sequencing libraries were constructed using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, Ipswich, MA, USA) according to manufacturer’s instructions. .. High-throughput sequencing was performed using Illumina HiSeq™ 4000 platform (Illumina Inc., San Diego, CA, USA) in paired-end mode with the read length of 125 bp after the library quality was assessed by Agilent Bioanalyzer 2100 system.

    Article Title: Expression profile analysis of 5-day-old neonatal piglets infected with porcine Deltacoronavirus
    Article Snippet: .. Next, Poly (A) mRNA was isolated using oligo-dT beads (Qiagen). ..

    Article Title: Genome-wide transcriptome analysis reveals the molecular mechanism of high temperature-induced floral abortion in Litchi chinensis
    Article Snippet: RNA extraction, library construction, and RNA-sequencing Total RNA extraction was carried out using Plant Total RNA Isolation kit (Huayueyang, Beijing, China). .. Oligo-dT beads (Qiagen, Valencia, USA) was used to enrich mRNA.

    Article Title: Comparative transcriptome analysis of two selenium-accumulating genotypes of Aegilops tauschii Coss. in response to selenium
    Article Snippet: .. Poly (A) mRNA was isolated using oligo-dT beads (Qiagen) and broken into short fragments (200 nt) with fragmentation buffer. .. First-strand cDNA was generated by random hexamer-primed reverse transcription using the fragmented mRNA as a template, with second-strand cDNA then synthesized after addition of buffer, dNTPs, RNase H, and DNA polymerase I.

    Article Title: Transcriptome profiling in the spathe of Anthurium andraeanum ‘Albama’ and its anthocyanin-loss mutant ‘Xueyu’
    Article Snippet: .. Using a Poly(A)PuristTM Kit (Ambion, now Life Technologies) and oligo-dT beads (Qiagen), the mRNA was isolated. .. Then the mRNA was fragmented and was used as a template to synthesize cDNA using a PrimeScript 1st Strand cDNA Synthesis Kit (Takara).

    Article Title: Transcriptomics reveals multiple resistance mechanisms against cotton leaf curl disease in a naturally immune cotton species, Gossypium arboreum
    Article Snippet: .. Poly (A) mRNA was isolated using oligo-dT beads (Qiagen, Hilden, Germany). ..

    Article Title: Gene expression dynamics during the gonocyte to spermatogonia transition and spermatogenesis in the domestic yak
    Article Snippet: .. Next, Poly (A) mRNA was isolated using oligo-dT beads (Qiagen). ..

    Article Title: Transcriptomic Profiles of Brain Provide Insights into Molecular Mechanism of Feed Conversion Efficiency in Crucian Carp (Carassius auratus)
    Article Snippet: Total RNA was isolated from the frozen brain samples using Trizol Reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. .. Oligo-dT beads (Qiagen, Dusseldorf, Germany) were used to separate poly (A) mRNA from the total RNA of each sample, and the fragmentation buffer was added to split all mRNA into short fragments.

    Article Title: Transcriptome Analysis of Larval Segment Formation and Secondary Loss in the Echiuran Worm Urechis unicinctus
    Article Snippet: .. The mRNA was isolated using oligo-dT beads (Qiagen, Hilden, Germany) and then broken into short fragments by adding fragmentation buffer. .. The first-strand cDNA was generated using random hexamer-primer with the short fragments as templates.

    Article Title: The First Complete Genome Sequence of a Novel Tetrastichus brontispae RNA Virus-1 (TbRV-1)
    Article Snippet: .. Poly (A) mRNA was isolated using oligo-dT beads (Qiagen, Tokyo, Japan). ..

    Ligation:

    Article Title: Transcriptome profiling in the spathe of Anthurium andraeanum ‘Albama’ and its anthocyanin-loss mutant ‘Xueyu’
    Article Snippet: Using a Poly(A)PuristTM Kit (Ambion, now Life Technologies) and oligo-dT beads (Qiagen), the mRNA was isolated. .. The cDNA was purified and subjected to end preparation, single nucleotide adenine addition and adaptor ligation.

    Sequencing:

    Article Title: De novo Transcriptome Assembly of Floral Buds of Pineapple and Identification of Differentially Expressed Genes in Response to Ethephon Induction
    Article Snippet: Paragraph title: RNA-seq library construction and sequencing ... Poly (A) mRNA was isolated using oligo-dT beads (Qiagen).

    Article Title: Comparative Transcriptomic Analysis Reveals Candidate Genes and Pathways Involved in Larval Settlement of the Barnacle Megabalanus volcano
    Article Snippet: .. The mRNA was isolated with oligo-dT beads (Qiagen, Hilden, Germany), and then sequencing libraries were constructed using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, Ipswich, MA, USA) according to manufacturer’s instructions. .. High-throughput sequencing was performed using Illumina HiSeq™ 4000 platform (Illumina Inc., San Diego, CA, USA) in paired-end mode with the read length of 125 bp after the library quality was assessed by Agilent Bioanalyzer 2100 system.

    Article Title: Transcriptome Profile Analysis on Ovarian Tissues of Autotetraploid Fish and Diploid Red Crucian Carp
    Article Snippet: Paragraph title: RNA Sequencing Library Construction and Illumina Sequencing ... Poly (A) was separated using oligo-dT beads (Qiagen, Dusseldorf, Germany).

    Article Title: Expression profile analysis of 5-day-old neonatal piglets infected with porcine Deltacoronavirus
    Article Snippet: Paragraph title: RNA extraction, library construction and sequencing ... Next, Poly (A) mRNA was isolated using oligo-dT beads (Qiagen).

    Article Title: Comparative transcriptome analysis of two selenium-accumulating genotypes of Aegilops tauschii Coss. in response to selenium
    Article Snippet: Paragraph title: RNA extraction and RNA-Seq library construction and sequencing ... Poly (A) mRNA was isolated using oligo-dT beads (Qiagen) and broken into short fragments (200 nt) with fragmentation buffer.

    Article Title: Transcriptome profiling in the spathe of Anthurium andraeanum ‘Albama’ and its anthocyanin-loss mutant ‘Xueyu’
    Article Snippet: The sequencing work was performed by BGI Life Tech Co., Ltd (Shenzhen, China). .. Using a Poly(A)PuristTM Kit (Ambion, now Life Technologies) and oligo-dT beads (Qiagen), the mRNA was isolated.

    Article Title: Transcriptome Profile Analysis of Ovarian Tissues from Diploid and Tetraploid Loaches Misgurnus anguillicaudatus
    Article Snippet: Paragraph title: 4.3. Library Construction and Illumina Sequencing ... Poly (A) mRNA was segregated using oligo-dT beads (Qiagen, Dusseldorf, Germany).

    Article Title: Gene expression dynamics during the gonocyte to spermatogonia transition and spermatogenesis in the domestic yak
    Article Snippet: Paragraph title: RNA extraction, library construction and sequencing ... Next, Poly (A) mRNA was isolated using oligo-dT beads (Qiagen).

    Article Title: Hepatic transcriptome analysis and identification of differentially expressed genes response to dietary oxidized fish oil in loach Misgurnus anguillicaudatus
    Article Snippet: Paragraph title: RNA extraction, cDNA library and illumina sequencing ... Poly (A) mRNA was separated using oligo-dT beads (Qiagen, Dusseldorf, Germany).

    Article Title: Transcriptomic Profiles of Brain Provide Insights into Molecular Mechanism of Feed Conversion Efficiency in Crucian Carp (Carassius auratus)
    Article Snippet: Paragraph title: 4.2. RNA Extraction, Library Preparation and Transcriptome Sequencing ... Oligo-dT beads (Qiagen, Dusseldorf, Germany) were used to separate poly (A) mRNA from the total RNA of each sample, and the fragmentation buffer was added to split all mRNA into short fragments.

    Article Title: Transcriptome Analysis of Larval Segment Formation and Secondary Loss in the Echiuran Worm Urechis unicinctus
    Article Snippet: Paragraph title: 4.3. RNA Extraction, RNA-Seq Library Construction, and Sequencing ... The mRNA was isolated using oligo-dT beads (Qiagen, Hilden, Germany) and then broken into short fragments by adding fragmentation buffer.

    Generated:

    Article Title: De novo Transcriptome Assembly of Floral Buds of Pineapple and Identification of Differentially Expressed Genes in Response to Ethephon Induction
    Article Snippet: Poly (A) mRNA was isolated using oligo-dT beads (Qiagen). .. First-strand cDNA was synthesized by random hexamer-primed reverse transcription, and then second-strand cDNA was generated using RNase H and DNA polymerase I.

    Article Title: Expression profile analysis of 5-day-old neonatal piglets infected with porcine Deltacoronavirus
    Article Snippet: Next, Poly (A) mRNA was isolated using oligo-dT beads (Qiagen). .. First-strand cDNA was generated using random hexamer-primed reverse transcription, and then primitive fragmented mRNA was digested by RNase H, followed by the synthesis of the second-strand cDNA.

    Article Title: Comparative transcriptome analysis of two selenium-accumulating genotypes of Aegilops tauschii Coss. in response to selenium
    Article Snippet: Poly (A) mRNA was isolated using oligo-dT beads (Qiagen) and broken into short fragments (200 nt) with fragmentation buffer. .. First-strand cDNA was generated by random hexamer-primed reverse transcription using the fragmented mRNA as a template, with second-strand cDNA then synthesized after addition of buffer, dNTPs, RNase H, and DNA polymerase I.

    Article Title: Transcriptomics reveals multiple resistance mechanisms against cotton leaf curl disease in a naturally immune cotton species, Gossypium arboreum
    Article Snippet: Poly (A) mRNA was isolated using oligo-dT beads (Qiagen, Hilden, Germany). .. Second-strand cDNA was generated using RNase H and DNA polymerase I.

    Article Title: Gene expression dynamics during the gonocyte to spermatogonia transition and spermatogenesis in the domestic yak
    Article Snippet: Next, Poly (A) mRNA was isolated using oligo-dT beads (Qiagen). .. First-strand cDNA was generated using random hexamer-primed reverse transcription, followed by the synthesis of the second-strand cDNA using RNase H and DNA polymerase I.

    Article Title: Transcriptomic Profiles of Brain Provide Insights into Molecular Mechanism of Feed Conversion Efficiency in Crucian Carp (Carassius auratus)
    Article Snippet: Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, Ipswich, MA, USA) according to manufacturer’s recommendations. .. Oligo-dT beads (Qiagen, Dusseldorf, Germany) were used to separate poly (A) mRNA from the total RNA of each sample, and the fragmentation buffer was added to split all mRNA into short fragments.

    Article Title: Transcriptome Analysis of Larval Segment Formation and Secondary Loss in the Echiuran Worm Urechis unicinctus
    Article Snippet: The mRNA was isolated using oligo-dT beads (Qiagen, Hilden, Germany) and then broken into short fragments by adding fragmentation buffer. .. The first-strand cDNA was generated using random hexamer-primer with the short fragments as templates.

    Article Title: The First Complete Genome Sequence of a Novel Tetrastichus brontispae RNA Virus-1 (TbRV-1)
    Article Snippet: Poly (A) mRNA was isolated using oligo-dT beads (Qiagen, Tokyo, Japan). .. First-strand cDNA was generated using random hexamer-primed reverse transcription, followed by the synthesis of the second-strand cDNA using RNase H and DNA polymerase I.

    Construct:

    Article Title: Comparative Transcriptomic Analysis Reveals Candidate Genes and Pathways Involved in Larval Settlement of the Barnacle Megabalanus volcano
    Article Snippet: .. The mRNA was isolated with oligo-dT beads (Qiagen, Hilden, Germany), and then sequencing libraries were constructed using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, Ipswich, MA, USA) according to manufacturer’s instructions. .. High-throughput sequencing was performed using Illumina HiSeq™ 4000 platform (Illumina Inc., San Diego, CA, USA) in paired-end mode with the read length of 125 bp after the library quality was assessed by Agilent Bioanalyzer 2100 system.

    Article Title: Transcriptome Profile Analysis on Ovarian Tissues of Autotetraploid Fish and Diploid Red Crucian Carp
    Article Snippet: RNA Sequencing Library Construction and Illumina Sequencing The cDNA library was constructed using high quality RNA. .. Poly (A) was separated using oligo-dT beads (Qiagen, Dusseldorf, Germany).

    Article Title: Comparative transcriptome analysis of two selenium-accumulating genotypes of Aegilops tauschii Coss. in response to selenium
    Article Snippet: Poly (A) mRNA was isolated using oligo-dT beads (Qiagen) and broken into short fragments (200 nt) with fragmentation buffer. .. The constructed cDNA library was sequenced in a single run on the IlluminaHiSeq 2500 platform using single-end and paired-end technology at Beijing Genomics Institute (Shenzhen, China).

    Article Title: Transcriptome Profile Analysis of Ovarian Tissues from Diploid and Tetraploid Loaches Misgurnus anguillicaudatus
    Article Snippet: Library Construction and Illumina Sequencing The cDNA library was constructed by using the extracted RNA samples. .. Poly (A) mRNA was segregated using oligo-dT beads (Qiagen, Dusseldorf, Germany).

    Article Title: Transcriptomics reveals multiple resistance mechanisms against cotton leaf curl disease in a naturally immune cotton species, Gossypium arboreum
    Article Snippet: Library construction and RNA sequencing The extracted total RNA samples with a concentration of 10 µg were used for cDNA synthesis and strand specific RNA-Seq libraries were constructed as described earlier . .. Poly (A) mRNA was isolated using oligo-dT beads (Qiagen, Hilden, Germany).

    Article Title: Transcriptomic Profiles of Brain Provide Insights into Molecular Mechanism of Feed Conversion Efficiency in Crucian Carp (Carassius auratus)
    Article Snippet: Oligo-dT beads (Qiagen, Dusseldorf, Germany) were used to separate poly (A) mRNA from the total RNA of each sample, and the fragmentation buffer was added to split all mRNA into short fragments. .. Fragments with suitable size for sequencing were isolated from the agarose gels, and PCR amplification was used to enrich these fragments to construct the final cDNA libraries.

    Article Title: The First Complete Genome Sequence of a Novel Tetrastichus brontispae RNA Virus-1 (TbRV-1)
    Article Snippet: The complementary DNA (cDNA) library was constructed with NEBNext® Ultra RNA Library Prep Kit (New England Biolabs, Ipswich, MA, USA). .. Poly (A) mRNA was isolated using oligo-dT beads (Qiagen, Tokyo, Japan).

    Purification:

    Article Title: De novo Transcriptome Assembly of Floral Buds of Pineapple and Identification of Differentially Expressed Genes in Response to Ethephon Induction
    Article Snippet: Poly (A) mRNA was isolated using oligo-dT beads (Qiagen). .. The cDNA fragments were purified using a QIAquick PCR Extraction Kit.

    Article Title: Genome-wide transcriptome analysis reveals the molecular mechanism of high temperature-induced floral abortion in Litchi chinensis
    Article Snippet: Oligo-dT beads (Qiagen, Valencia, USA) was used to enrich mRNA. .. After that, the cDNA fragments were purified by Qiaquick PCR Purification Kits (Qiagen, Valencia, USA).

    Article Title: Comparative transcriptome analysis of two selenium-accumulating genotypes of Aegilops tauschii Coss. in response to selenium
    Article Snippet: The quality of the purified RNA was verified by agarose gel electrophoresis, and its concentration was measured on a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) at 260 nm and 280 nm. .. Poly (A) mRNA was isolated using oligo-dT beads (Qiagen) and broken into short fragments (200 nt) with fragmentation buffer.

    Article Title: Transcriptome profiling in the spathe of Anthurium andraeanum ‘Albama’ and its anthocyanin-loss mutant ‘Xueyu’
    Article Snippet: Using a Poly(A)PuristTM Kit (Ambion, now Life Technologies) and oligo-dT beads (Qiagen), the mRNA was isolated. .. The cDNA was purified and subjected to end preparation, single nucleotide adenine addition and adaptor ligation.

    Article Title: Gene expression dynamics during the gonocyte to spermatogonia transition and spermatogenesis in the domestic yak
    Article Snippet: Next, Poly (A) mRNA was isolated using oligo-dT beads (Qiagen). .. The cDNA fragments were purified using a QIA quick PCR extraction kit.

    Article Title: Hepatic transcriptome analysis and identification of differentially expressed genes response to dietary oxidized fish oil in loach Misgurnus anguillicaudatus
    Article Snippet: To obtain purified mRNA, total RNA was incubated with 10 U DNase I (Ambion, Grand Island, NY) at 37°C for 1 h, followed by a purification step using a MicroPoly (A) Purist Kit (Ambion, Grand Island, NY) according to the manufacturer’s instructions. .. Poly (A) mRNA was separated using oligo-dT beads (Qiagen, Dusseldorf, Germany).

    Article Title: Transcriptomic Profiles of Brain Provide Insights into Molecular Mechanism of Feed Conversion Efficiency in Crucian Carp (Carassius auratus)
    Article Snippet: Oligo-dT beads (Qiagen, Dusseldorf, Germany) were used to separate poly (A) mRNA from the total RNA of each sample, and the fragmentation buffer was added to split all mRNA into short fragments. .. The cDNA fragments were washed by EB buffer for end reparation poly (A) after purification, and index codes were added to attribute sequences to each sample.

    Article Title: Transcriptome Analysis of Larval Segment Formation and Secondary Loss in the Echiuran Worm Urechis unicinctus
    Article Snippet: The mRNA was isolated using oligo-dT beads (Qiagen, Hilden, Germany) and then broken into short fragments by adding fragmentation buffer. .. The cDNA fragments were purified using a QiaQuick PCR extraction kit (Qiagen, Hilden, Germany).

    Article Title: The First Complete Genome Sequence of a Novel Tetrastichus brontispae RNA Virus-1 (TbRV-1)
    Article Snippet: Poly (A) mRNA was isolated using oligo-dT beads (Qiagen, Tokyo, Japan). .. The cDNA fragments were purified using a QIAquick PCR extraction kit.

    Real-time Polymerase Chain Reaction:

    Article Title: Transcriptome profiling in the spathe of Anthurium andraeanum ‘Albama’ and its anthocyanin-loss mutant ‘Xueyu’
    Article Snippet: Using a Poly(A)PuristTM Kit (Ambion, now Life Technologies) and oligo-dT beads (Qiagen), the mRNA was isolated. .. After quality control with an Agilent 2100 Bioanalyzer and ABI StepOnePlus Real-Time PCR System, the library was sequenced using Illumina HiSeqTM 4000.

    Polymerase Chain Reaction:

    Article Title: De novo Transcriptome Assembly of Floral Buds of Pineapple and Identification of Differentially Expressed Genes in Response to Ethephon Induction
    Article Snippet: Poly (A) mRNA was isolated using oligo-dT beads (Qiagen). .. The cDNA fragments were purified using a QIAquick PCR Extraction Kit.

    Article Title: Genome-wide transcriptome analysis reveals the molecular mechanism of high temperature-induced floral abortion in Litchi chinensis
    Article Snippet: Oligo-dT beads (Qiagen, Valencia, USA) was used to enrich mRNA. .. After that, the cDNA fragments were purified by Qiaquick PCR Purification Kits (Qiagen, Valencia, USA).

    Article Title: Comparative transcriptome analysis of two selenium-accumulating genotypes of Aegilops tauschii Coss. in response to selenium
    Article Snippet: Poly (A) mRNA was isolated using oligo-dT beads (Qiagen) and broken into short fragments (200 nt) with fragmentation buffer. .. The generated cDNA fragments were purified using a QIAquick PCR gel extraction kit (Qiagen, Germany), washed with EB, subjected to end reparation and poly (A) addition, and ligated to sequencing adapters. cDNA fragments of interest (200 ± 25 bp) were purified and enriched by PCR amplification to complete preparation of the entire cDNA library.

    Article Title: Gene expression dynamics during the gonocyte to spermatogonia transition and spermatogenesis in the domestic yak
    Article Snippet: Next, Poly (A) mRNA was isolated using oligo-dT beads (Qiagen). .. The cDNA fragments were purified using a QIA quick PCR extraction kit.

    Article Title: Transcriptomic Profiles of Brain Provide Insights into Molecular Mechanism of Feed Conversion Efficiency in Crucian Carp (Carassius auratus)
    Article Snippet: Oligo-dT beads (Qiagen, Dusseldorf, Germany) were used to separate poly (A) mRNA from the total RNA of each sample, and the fragmentation buffer was added to split all mRNA into short fragments. .. Fragments with suitable size for sequencing were isolated from the agarose gels, and PCR amplification was used to enrich these fragments to construct the final cDNA libraries.

    Article Title: Transcriptome Analysis of Larval Segment Formation and Secondary Loss in the Echiuran Worm Urechis unicinctus
    Article Snippet: The mRNA was isolated using oligo-dT beads (Qiagen, Hilden, Germany) and then broken into short fragments by adding fragmentation buffer. .. The cDNA fragments were purified using a QiaQuick PCR extraction kit (Qiagen, Hilden, Germany).

    Article Title: The First Complete Genome Sequence of a Novel Tetrastichus brontispae RNA Virus-1 (TbRV-1)
    Article Snippet: Poly (A) mRNA was isolated using oligo-dT beads (Qiagen, Tokyo, Japan). .. The cDNA fragments were purified using a QIAquick PCR extraction kit.

    Incubation:

    Article Title: Hepatic transcriptome analysis and identification of differentially expressed genes response to dietary oxidized fish oil in loach Misgurnus anguillicaudatus
    Article Snippet: To obtain purified mRNA, total RNA was incubated with 10 U DNase I (Ambion, Grand Island, NY) at 37°C for 1 h, followed by a purification step using a MicroPoly (A) Purist Kit (Ambion, Grand Island, NY) according to the manufacturer’s instructions. .. Poly (A) mRNA was separated using oligo-dT beads (Qiagen, Dusseldorf, Germany).

    cDNA Library Assay:

    Article Title: Transcriptome Profile Analysis on Ovarian Tissues of Autotetraploid Fish and Diploid Red Crucian Carp
    Article Snippet: RNA Sequencing Library Construction and Illumina Sequencing The cDNA library was constructed using high quality RNA. .. Poly (A) was separated using oligo-dT beads (Qiagen, Dusseldorf, Germany).

    Article Title: Comparative transcriptome analysis of two selenium-accumulating genotypes of Aegilops tauschii Coss. in response to selenium
    Article Snippet: Poly (A) mRNA was isolated using oligo-dT beads (Qiagen) and broken into short fragments (200 nt) with fragmentation buffer. .. The generated cDNA fragments were purified using a QIAquick PCR gel extraction kit (Qiagen, Germany), washed with EB, subjected to end reparation and poly (A) addition, and ligated to sequencing adapters. cDNA fragments of interest (200 ± 25 bp) were purified and enriched by PCR amplification to complete preparation of the entire cDNA library.

    Article Title: Transcriptome Profile Analysis of Ovarian Tissues from Diploid and Tetraploid Loaches Misgurnus anguillicaudatus
    Article Snippet: Library Construction and Illumina Sequencing The cDNA library was constructed by using the extracted RNA samples. .. Poly (A) mRNA was segregated using oligo-dT beads (Qiagen, Dusseldorf, Germany).

    Article Title: Hepatic transcriptome analysis and identification of differentially expressed genes response to dietary oxidized fish oil in loach Misgurnus anguillicaudatus
    Article Snippet: Paragraph title: RNA extraction, cDNA library and illumina sequencing ... Poly (A) mRNA was separated using oligo-dT beads (Qiagen, Dusseldorf, Germany).

    Article Title: Transcriptomic Profiles of Brain Provide Insights into Molecular Mechanism of Feed Conversion Efficiency in Crucian Carp (Carassius auratus)
    Article Snippet: The RNA integrity number was assessed using the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA), which ranged from 8.2 to 8.9, suggesting that the samples were well preserved to meet the cDNA library construction requirements. .. Oligo-dT beads (Qiagen, Dusseldorf, Germany) were used to separate poly (A) mRNA from the total RNA of each sample, and the fragmentation buffer was added to split all mRNA into short fragments.

    Article Title: The First Complete Genome Sequence of a Novel Tetrastichus brontispae RNA Virus-1 (TbRV-1)
    Article Snippet: The complementary DNA (cDNA) library was constructed with NEBNext® Ultra RNA Library Prep Kit (New England Biolabs, Ipswich, MA, USA). .. Poly (A) mRNA was isolated using oligo-dT beads (Qiagen, Tokyo, Japan).

    Spectrophotometry:

    Article Title: Transcriptomic Profiles of Brain Provide Insights into Molecular Mechanism of Feed Conversion Efficiency in Crucian Carp (Carassius auratus)
    Article Snippet: RNA purity and concentration was measured by NanoPhotometer® spectrophotometer (IMPLEN, Westlake Village, CA, USA) and Qubit® 2.0 Flurometer (Life Technologies, Carlsbad, CA, USA). .. Oligo-dT beads (Qiagen, Dusseldorf, Germany) were used to separate poly (A) mRNA from the total RNA of each sample, and the fragmentation buffer was added to split all mRNA into short fragments.

    High Throughput Screening Assay:

    Article Title: Comparative Transcriptomic Analysis Reveals Candidate Genes and Pathways Involved in Larval Settlement of the Barnacle Megabalanus volcano
    Article Snippet: The mRNA was isolated with oligo-dT beads (Qiagen, Hilden, Germany), and then sequencing libraries were constructed using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, Ipswich, MA, USA) according to manufacturer’s instructions. .. High-throughput sequencing was performed using Illumina HiSeq™ 4000 platform (Illumina Inc., San Diego, CA, USA) in paired-end mode with the read length of 125 bp after the library quality was assessed by Agilent Bioanalyzer 2100 system.

    Gel Extraction:

    Article Title: Comparative transcriptome analysis of two selenium-accumulating genotypes of Aegilops tauschii Coss. in response to selenium
    Article Snippet: Poly (A) mRNA was isolated using oligo-dT beads (Qiagen) and broken into short fragments (200 nt) with fragmentation buffer. .. The generated cDNA fragments were purified using a QIAquick PCR gel extraction kit (Qiagen, Germany), washed with EB, subjected to end reparation and poly (A) addition, and ligated to sequencing adapters. cDNA fragments of interest (200 ± 25 bp) were purified and enriched by PCR amplification to complete preparation of the entire cDNA library.

    Microarray:

    Article Title: Expression profile analysis of 5-day-old neonatal piglets infected with porcine Deltacoronavirus
    Article Snippet: RNA extraction, library construction and sequencing Samples of the jejunum closed to the ileum were collected from 18 experimental piglets for Microarray analysis. .. Next, Poly (A) mRNA was isolated using oligo-dT beads (Qiagen).

    Concentration Assay:

    Article Title: Comparative transcriptome analysis of two selenium-accumulating genotypes of Aegilops tauschii Coss. in response to selenium
    Article Snippet: The quality of the purified RNA was verified by agarose gel electrophoresis, and its concentration was measured on a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) at 260 nm and 280 nm. .. Poly (A) mRNA was isolated using oligo-dT beads (Qiagen) and broken into short fragments (200 nt) with fragmentation buffer.

    Article Title: Transcriptomics reveals multiple resistance mechanisms against cotton leaf curl disease in a naturally immune cotton species, Gossypium arboreum
    Article Snippet: Library construction and RNA sequencing The extracted total RNA samples with a concentration of 10 µg were used for cDNA synthesis and strand specific RNA-Seq libraries were constructed as described earlier . .. Poly (A) mRNA was isolated using oligo-dT beads (Qiagen, Hilden, Germany).

    Article Title: Gene expression dynamics during the gonocyte to spermatogonia transition and spermatogenesis in the domestic yak
    Article Snippet: RNA quality and concentration were measured by using Agilent 2100 Bio-analyzer (Agilent Technologies, Santa Clara, CA) and by RNase free agarose gel electrophoresis after removing the genomic DNA. .. Next, Poly (A) mRNA was isolated using oligo-dT beads (Qiagen).

    Article Title: Hepatic transcriptome analysis and identification of differentially expressed genes response to dietary oxidized fish oil in loach Misgurnus anguillicaudatus
    Article Snippet: The concentration of total RNA was determined by NanoDrop 2000 (Thermo Scientific, Waltham, MA), and the RNA integrity value was checked by the RNA 6000 Pico LabChip on an Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA). .. Poly (A) mRNA was separated using oligo-dT beads (Qiagen, Dusseldorf, Germany).

    Article Title: Transcriptomic Profiles of Brain Provide Insights into Molecular Mechanism of Feed Conversion Efficiency in Crucian Carp (Carassius auratus)
    Article Snippet: RNA purity and concentration was measured by NanoPhotometer® spectrophotometer (IMPLEN, Westlake Village, CA, USA) and Qubit® 2.0 Flurometer (Life Technologies, Carlsbad, CA, USA). .. Oligo-dT beads (Qiagen, Dusseldorf, Germany) were used to separate poly (A) mRNA from the total RNA of each sample, and the fragmentation buffer was added to split all mRNA into short fragments.

    Article Title: Transcriptome Analysis of Larval Segment Formation and Secondary Loss in the Echiuran Worm Urechis unicinctus
    Article Snippet: The concentration, purity, and integrity of the RNAs were assessed using NanoDrop 2000 (Thermo Scientific, Wilmington, DE, USA), and agarose gel electrophoresis were performed to assess the quality of total RNAs. .. The mRNA was isolated using oligo-dT beads (Qiagen, Hilden, Germany) and then broken into short fragments by adding fragmentation buffer.

    Article Title: The First Complete Genome Sequence of a Novel Tetrastichus brontispae RNA Virus-1 (TbRV-1)
    Article Snippet: The concentration and integrity of the total RNA were assessed by a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) before library construction. .. Poly (A) mRNA was isolated using oligo-dT beads (Qiagen, Tokyo, Japan).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 79
    Qiagen oligo dt latex bead chromatography
    Oligo Dt Latex Bead Chromatography, supplied by Qiagen, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oligo dt latex bead chromatography/product/Qiagen
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    oligo dt latex bead chromatography - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    90
    Qiagen oligo dt beads
    Oligo Dt Beads, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oligo dt beads/product/Qiagen
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    oligo dt beads - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    98
    Qiagen oligo dt magnetic beads
    Oligo Dt Magnetic Beads, supplied by Qiagen, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oligo dt magnetic beads/product/Qiagen
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    oligo dt magnetic beads - by Bioz Stars, 2020-02
    98/100 stars
      Buy from Supplier

    Image Search Results