odyssey dual channel infrared scanner  (LI-COR)


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    Structured Review

    LI-COR odyssey dual channel infrared scanner
    Odyssey Dual Channel Infrared Scanner, supplied by LI-COR, used in various techniques. Bioz Stars score: 87/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/odyssey dual channel infrared scanner/product/LI-COR
    Average 87 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    odyssey dual channel infrared scanner - by Bioz Stars, 2020-04
    87/100 stars

    Related Products / Commonly Used Together

    importin-α2
    htt
    p65

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    Related Articles

    Transduction:

    Article Title: The Huntington's disease mutation impairs Huntingtin's role in the transport of NF-\u03baB from the synapse to the nucleus
    Article Snippet: Primary antibodies included mouse monoclonal anti-CaMKIIα (Affinity BioReagents, Cat. No. MA1-048, 1:2500 dilution), mouse monoclonal anti-PSD-95 (Affinity BioReagents, Cat. No. MA1-048, 1:2500 dilution), mouse monoclonal anti-Synaptophysin (Sigma-Aldrich, Cat. No. S5768, 1:2500 dilution), rabbit polyclonal anti-synGAP (Affinity BioReagents, Cat. No. PA1-046, 1:2500 dilution), rabbit polyclonal anti-PSD-95 (custom made, Frances, 1:5000 dilution), mouse monoclonal anti-Htt (Chemicon, Cat. No. MAB2166, 1:2500 dilution), mouse monoclonal anti-clathrin heavy chain (Transduction Laboratories, Cat. No. 610499, 1:2500 dilution), mouse monoclonal anti-Importin-α2 (Transduction Laboratories, Cat. No. 610485, 1:5000 dilution), mouse monoclonal anti-HAP1 (Transduction Laboratories, Cat. No. 611302, 1:250 dilution); rabbit polyclonal anti-NF-κB p65 (Santa Cruz Biotechnology, Cat. No. sc-372, 1:1000 dilution), rabbit polyclonal anti-NF-κB p50 (Santa Cruz Biotechnology, Cat. No. sc-114, 1:1000 dilution), rabbit polyclonal anti-I-κBα (Santa Cruz Biotechnology, Cat. No. sc-371, 1:500 dilution), mouse monoclonal anti-I-κBα (Santa Cruz Biotechnology, Cat. No. sc-1643, 1:500 dilution), rabbit polyclonal anti-phospho-I-κBα S32 (Cell Signaling Technology, Cat. No. 9241, 1:1,000 dilution), mouse monoclonal anti-phospho-I-κBα S32/36 (Cell Signaling Technology, Cat. No. 9246, 1:1000 dilution) and mouse monoclonal anti-Lamin A/C (Cell Signaling Technology, Cat. No. 4777, 1:1000 dilution). .. For digital archiving and quantitative analysis, the membranes were scanned with an Odyssey dual-channel infrared scanner (LI-COR) using the Odyssey image analysis software (LI-COR).

    Centrifugation:

    Article Title: The Huntington's disease mutation impairs Huntingtin's role in the transport of NF-\u03baB from the synapse to the nucleus
    Article Snippet: Insoluble material was removed by brief centrifugation. .. To quantify the amount of precipitated protein, the integrated intensity of the fluorescent signal for p65 or importin-α2 that immunoprecipitated with Htt was measured with an Odyssey dual-channel infrared scanner and the Odyssey image analysis software (LI-COR).

    Binding Assay:

    Article Title: The Huntington's disease mutation impairs Huntingtin's role in the transport of NF-\u03baB from the synapse to the nucleus
    Article Snippet: After binding, the wells were washed three times with 200 µl of PBS + 0.1% Tween (PBS-Tw), the primary rabbit polyclonal antibody anti-PSD-95 (custom made, Frances, 1:1000 dilution) was added (100 µl per well, in Odyssey Blocking Buffer diluted with an equal volume of PBS-Tw) and the plate was incubated for 1 h at room temperature. .. The integrated intensity of the specific fluorescent signal was measured with an Odyssey dual-channel infrared scanner (LI-COR) using the Odyssey image analysis software (LI-COR) and interpreted as directly indicative of the amount of PSD-95 specifically bound to Htt exon 1.

    Protease Inhibitor:

    Article Title: The Huntington's disease mutation impairs Huntingtin's role in the transport of NF-\u03baB from the synapse to the nucleus
    Article Snippet: In total, 107 HeLa cells were lysed in 1 ml of ice-cold NP-40 lysis buffer [50 m m Tris–HCl, pH 7.5, 150 m m sodium chloride, 1% NP-40, 1× protease inhibitor cocktail (Sigma-Aldrich, Cat. No. P8340)] by incubation for 30 min on ice with occasional mixing. .. To quantify the amount of precipitated protein, the integrated intensity of the fluorescent signal for p65 or importin-α2 that immunoprecipitated with Htt was measured with an Odyssey dual-channel infrared scanner and the Odyssey image analysis software (LI-COR).

    Blocking Assay:

    Article Title: The Huntington's disease mutation impairs Huntingtin's role in the transport of NF-\u03baB from the synapse to the nucleus
    Article Snippet: The membranes were blocked overnight at 4°C in Odyssey Blocking Buffer (LI-COR) with continuous mixing and then incubated with primary and secondary antibodies according to manufacturer's instructions. .. For digital archiving and quantitative analysis, the membranes were scanned with an Odyssey dual-channel infrared scanner (LI-COR) using the Odyssey image analysis software (LI-COR).

    Article Title: The Huntington's disease mutation impairs Huntingtin's role in the transport of NF-\u03baB from the synapse to the nucleus
    Article Snippet: After incubation with the primary antibody, the wells were washed three times with 200 µl of PBS-Tw, the secondary Alexa Fluor 680 goat-anti-rabbit IgG antibody (Molecular Probes, 1:2000 dilution) was added (100 µl per well, in Odyssey Blocking Buffer diluted with an equal volume of PBS-Tw) and the plate was incubated for 30 min at room temperature. .. The integrated intensity of the specific fluorescent signal was measured with an Odyssey dual-channel infrared scanner (LI-COR) using the Odyssey image analysis software (LI-COR) and interpreted as directly indicative of the amount of PSD-95 specifically bound to Htt exon 1.

    Adsorption:

    Article Title: The Huntington's disease mutation impairs Huntingtin's role in the transport of NF-\u03baB from the synapse to the nucleus
    Article Snippet: After adsorption, the wells were washed three times with 200 µl of PBS and blocked overnight at 4°C with 200 µl of Odyssey Blocking Buffer (LI-COR). .. The integrated intensity of the specific fluorescent signal was measured with an Odyssey dual-channel infrared scanner (LI-COR) using the Odyssey image analysis software (LI-COR) and interpreted as directly indicative of the amount of PSD-95 specifically bound to Htt exon 1.

    Immunoprecipitation:

    Article Title: The Huntington's disease mutation impairs Huntingtin's role in the transport of NF-\u03baB from the synapse to the nucleus
    Article Snippet: .. To quantify the amount of precipitated protein, the integrated intensity of the fluorescent signal for p65 or importin-α2 that immunoprecipitated with Htt was measured with an Odyssey dual-channel infrared scanner and the Odyssey image analysis software (LI-COR). ..

    Incubation:

    Article Title: The Huntington's disease mutation impairs Huntingtin's role in the transport of NF-\u03baB from the synapse to the nucleus
    Article Snippet: The membranes were blocked overnight at 4°C in Odyssey Blocking Buffer (LI-COR) with continuous mixing and then incubated with primary and secondary antibodies according to manufacturer's instructions. .. For digital archiving and quantitative analysis, the membranes were scanned with an Odyssey dual-channel infrared scanner (LI-COR) using the Odyssey image analysis software (LI-COR).

    Article Title: The Huntington's disease mutation impairs Huntingtin's role in the transport of NF-\u03baB from the synapse to the nucleus
    Article Snippet: Five hundred microliters of solubilized PSD fraction or HeLa cell lysate were incubated with the beads for at least 3 h at 4°C with continuous mixing. .. To quantify the amount of precipitated protein, the integrated intensity of the fluorescent signal for p65 or importin-α2 that immunoprecipitated with Htt was measured with an Odyssey dual-channel infrared scanner and the Odyssey image analysis software (LI-COR).

    Article Title: The Huntington's disease mutation impairs Huntingtin's role in the transport of NF-\u03baB from the synapse to the nucleus
    Article Snippet: After incubation with the secondary antibody, the wells were washed three times with 200 µl of PBS-Tw and once with 200 µl of PBS. .. The integrated intensity of the specific fluorescent signal was measured with an Odyssey dual-channel infrared scanner (LI-COR) using the Odyssey image analysis software (LI-COR) and interpreted as directly indicative of the amount of PSD-95 specifically bound to Htt exon 1.

    Marker:

    Article Title: The Huntington's disease mutation impairs Huntingtin's role in the transport of NF-\u03baB from the synapse to the nucleus
    Article Snippet: The All Blue Precision Plus Protein (Bio-Rad) prestained standard was used as molecular weight marker. .. For digital archiving and quantitative analysis, the membranes were scanned with an Odyssey dual-channel infrared scanner (LI-COR) using the Odyssey image analysis software (LI-COR).

    Lysis:

    Article Title: The Huntington's disease mutation impairs Huntingtin's role in the transport of NF-\u03baB from the synapse to the nucleus
    Article Snippet: The beads were then washed four times with 1 ml of ice-cold lysis buffer and once with 1 ml of ice-cold PBS using a magnetic separation rack (New England BioLabs). .. To quantify the amount of precipitated protein, the integrated intensity of the fluorescent signal for p65 or importin-α2 that immunoprecipitated with Htt was measured with an Odyssey dual-channel infrared scanner and the Odyssey image analysis software (LI-COR).

    Purification:

    Article Title: The Huntington's disease mutation impairs Huntingtin's role in the transport of NF-\u03baB from the synapse to the nucleus
    Article Snippet: After blocking, the wells were washed three times with 200 µl of PBS and various amounts of purified PSD-95 were then added (100 µl per well, in Odyssey Blocking Buffer diluted with an equal volume of PBS) in triplicates and allowed to interact with the ligand for 3 h at room temperature. .. The integrated intensity of the specific fluorescent signal was measured with an Odyssey dual-channel infrared scanner (LI-COR) using the Odyssey image analysis software (LI-COR) and interpreted as directly indicative of the amount of PSD-95 specifically bound to Htt exon 1.

    SDS Page:

    Article Title: The Huntington's disease mutation impairs Huntingtin's role in the transport of NF-\u03baB from the synapse to the nucleus
    Article Snippet: Equal amounts of total protein from each sample were resolved on 8% minigels by SDS–PAGE and transferred to Immobilon-FL membranes (Millipore) ( ). .. For digital archiving and quantitative analysis, the membranes were scanned with an Odyssey dual-channel infrared scanner (LI-COR) using the Odyssey image analysis software (LI-COR).

    Article Title: The Huntington's disease mutation impairs Huntingtin's role in the transport of NF-\u03baB from the synapse to the nucleus
    Article Snippet: The proteins bound to the beads were eluted with 50 µl of SDS–PAGE sample buffer and processed for immunoblotting as described above. .. To quantify the amount of precipitated protein, the integrated intensity of the fluorescent signal for p65 or importin-α2 that immunoprecipitated with Htt was measured with an Odyssey dual-channel infrared scanner and the Odyssey image analysis software (LI-COR).

    Molecular Weight:

    Article Title: The Huntington's disease mutation impairs Huntingtin's role in the transport of NF-\u03baB from the synapse to the nucleus
    Article Snippet: The All Blue Precision Plus Protein (Bio-Rad) prestained standard was used as molecular weight marker. .. For digital archiving and quantitative analysis, the membranes were scanned with an Odyssey dual-channel infrared scanner (LI-COR) using the Odyssey image analysis software (LI-COR).

    Software:

    Article Title: The Huntington's disease mutation impairs Huntingtin's role in the transport of NF-\u03baB from the synapse to the nucleus
    Article Snippet: .. For digital archiving and quantitative analysis, the membranes were scanned with an Odyssey dual-channel infrared scanner (LI-COR) using the Odyssey image analysis software (LI-COR). .. Fifty microliters of protein G magnetic beads (Dynabeads Protein G, DYNAL/Invitrogen) were washed and coupled with 5 µg of antigen-specific or isotype control antibody according to manufacturer's instructions.

    Article Title: The Huntington's disease mutation impairs Huntingtin's role in the transport of NF-\u03baB from the synapse to the nucleus
    Article Snippet: .. To quantify the amount of precipitated protein, the integrated intensity of the fluorescent signal for p65 or importin-α2 that immunoprecipitated with Htt was measured with an Odyssey dual-channel infrared scanner and the Odyssey image analysis software (LI-COR). ..

    Article Title: The Huntington's disease mutation impairs Huntingtin's role in the transport of NF-\u03baB from the synapse to the nucleus
    Article Snippet: .. The integrated intensity of the specific fluorescent signal was measured with an Odyssey dual-channel infrared scanner (LI-COR) using the Odyssey image analysis software (LI-COR) and interpreted as directly indicative of the amount of PSD-95 specifically bound to Htt exon 1. .. The K d of the interaction between PSD-95 and Htt exon 1 was calculated by curve fitting using the Prism software (GraphPad).

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  • About
  • News
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  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 87
    LI-COR odyssey dual channel infrared scanner
    Odyssey Dual Channel Infrared Scanner, supplied by LI-COR, used in various techniques. Bioz Stars score: 87/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/odyssey dual channel infrared scanner/product/LI-COR
    Average 87 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    odyssey dual channel infrared scanner - by Bioz Stars, 2020-04
    87/100 stars
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