Journal: Scientific Reports
Article Title: The photocycle of orange carotenoid protein conceals distinct intermediates and asynchronous changes in the carotenoid and protein components
Figure Lengend Snippet: ( A ) flash-induced transitions of OCP absorption at 550 nm approximated by multiexponential decay: OCP – red, OCP in 1 M phosphate – grey; OCP in the presence of FRP (1/1.6 concentration ratio) – blue; ΔNTE OCP in the presence of FRP (1/1.6 concentration ratio) – black. Note the logarithmic timescale covering almost 7 orders of magnitude. ( B ) dependency of the photoconversion amplitude on the energy of the laser flash for OCP in the presence of FRP (1/1.6 concentration ratio). ( C ) Arrhenius plot of the fast (C1) and the slow (C3) components’ rate constants of absorption changes of OCP in the presence of FRP. Experiments were conducted in the range of temperatures from 6 to 37 °C.
Article Snippet: Time-Correlated Single-Photon Counting (TCSPC) techniques for tryptophan fluorescence transients In all experiments, OCP photoconversion was triggered by a blue LED (M455L3, Thorlabs, USA), which passed through the FB450-40 bandpass filter (Thorlabs, USA).
Techniques: Concentration Assay