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R-Biopharm ochraprep immunoaffinity columns
Ochraprep Immunoaffinity Columns, supplied by R-Biopharm, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ochraprep immunoaffinity columns/product/R-Biopharm
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
ochraprep immunoaffinity columns - by Bioz Stars, 2021-03
86/100 stars

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Purification:

Article Title: Antifungal and Antiochratoxigenic Activities of Essential Oils and Total Phenolic Extracts: A Comparative Study
Article Snippet: After centrifugation at 12,054 g, the phase containing the OTA was separated from the debris and diluted with 20 mL phosphate buffer in order to prepare the extract to be purified with immunoaffinity columns. .. The purification process starts by injecting the diluted OTA into Ochraprep immunoaffinity columns (R-Biopharm, Glasgow, Scotland) using a syringe. ..

Article Title: OTA Prevention and Detoxification by Actinobacterial Strains and Activated Carbon Fibers: Preliminary Results
Article Snippet: .. Dry extracts were re-suspended with 2 mL of methanol and purified with Ochraprep Immunoaffinity columns (R-Biopharm, Glasgow, UK) following the manufacturer’s instructions before being subjected to HPLC/FLD/DAD analysis for OTA quantification. .. OTA Degradation Assay by Actinobacteria Bacteria were inoculated on ISP2 medium supplemented with OTA at 100 µg/L using a streak from a five-day old solid pre-culture.

Article Title: Ability of Soil Isolated Actinobacterial Strains to Prevent, Bind and Biodegrade Ochratoxin A
Article Snippet: At 0, 30 and 60 min, tubes were centrifuged and 1 mL of the supernatant was purified with Ochraprep Immunoaffinity columns and quantified with Waters Alliance HPLC system (Milford, MA 01757 USA)). .. As for the pellets, they were washed with methanol acidified with acetic acid (98:2 v/v ), purified with Ochraprep Immunoaffinity columns (R-Biopharm, Glasgow, UK) and quantified with HPLC. .. Detoxification Activity of the Actinobacteria The spores of the actinobacterial strains were scrapped from the pre-culture surfaces and added to 20 mL of liquid ISP2 medium, artificially contaminated with OTA solution.

Article Title: Ability of Soil Isolated Actinobacterial Strains to Prevent, Bind and Biodegrade Ochratoxin A
Article Snippet: After the incubation period, the culture tubes were shaken and then centrifuged at 13,000 rpm. .. A volume of 1 mL of the supernatant was removed and purified with Ochraprep Immunoaffinity columns (R-Biopharm, Glasgow, UK) following the manufacturer’s protocol, and analyzed by HPLC. ..

Article Title: Essential Oils Modulate Gene Expression and Ochratoxin A Production in Aspergillus carbonarius
Article Snippet: After centrifugation at 13,000 r.p.m (round per minute), the aqueous phase was separated from the debris and diluted with 20 mL phosphate buffer. .. OTA was then purified using Ochraprep immunoaffinity columns (R-Biopharm, Glasgow, Scotland) by injecting the mix into the columns using a syringe. ..

Article Title: Ability of Soil Isolated Actinobacterial Strains to Prevent, Bind and Biodegrade Ochratoxin A
Article Snippet: OTA was extracted by adding 1 mL of methanol and shaken for 1 h. After the incubation period, the mix was centrifuged at 13,000 rpm (revolutions per minute) for 20 min. .. The supernatant containing the OTA was then purified with Ochraprep immunoaffinity columns (R-Biopharm, Glasgow, UK) following the manufacturer’s protocol. .. HPLC Analysis The concentration of the purified OTA was evaluated with the Water Alliance HPLC system using an Utisphere ODB column, C18 (150 × 4.6 mm, 5 µm, 120 Å) (Interchim, Montluçon, France) at 30 °C.

High Performance Liquid Chromatography:

Article Title: OTA Prevention and Detoxification by Actinobacterial Strains and Activated Carbon Fibers: Preliminary Results
Article Snippet: .. Dry extracts were re-suspended with 2 mL of methanol and purified with Ochraprep Immunoaffinity columns (R-Biopharm, Glasgow, UK) following the manufacturer’s instructions before being subjected to HPLC/FLD/DAD analysis for OTA quantification. .. OTA Degradation Assay by Actinobacteria Bacteria were inoculated on ISP2 medium supplemented with OTA at 100 µg/L using a streak from a five-day old solid pre-culture.

Article Title: Ability of Soil Isolated Actinobacterial Strains to Prevent, Bind and Biodegrade Ochratoxin A
Article Snippet: At 0, 30 and 60 min, tubes were centrifuged and 1 mL of the supernatant was purified with Ochraprep Immunoaffinity columns and quantified with Waters Alliance HPLC system (Milford, MA 01757 USA)). .. As for the pellets, they were washed with methanol acidified with acetic acid (98:2 v/v ), purified with Ochraprep Immunoaffinity columns (R-Biopharm, Glasgow, UK) and quantified with HPLC. .. Detoxification Activity of the Actinobacteria The spores of the actinobacterial strains were scrapped from the pre-culture surfaces and added to 20 mL of liquid ISP2 medium, artificially contaminated with OTA solution.

Article Title: Ability of Soil Isolated Actinobacterial Strains to Prevent, Bind and Biodegrade Ochratoxin A
Article Snippet: After the incubation period, the culture tubes were shaken and then centrifuged at 13,000 rpm. .. A volume of 1 mL of the supernatant was removed and purified with Ochraprep Immunoaffinity columns (R-Biopharm, Glasgow, UK) following the manufacturer’s protocol, and analyzed by HPLC. ..

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    R-Biopharm ochraprep immunoaffinity columns
    Ochraprep Immunoaffinity Columns, supplied by R-Biopharm, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ochraprep immunoaffinity columns/product/R-Biopharm
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ochraprep immunoaffinity columns - by Bioz Stars, 2021-03
    86/100 stars
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