oceanimonas doudoroffi atcc 27123  (ATCC)

Journal: Life

Article Title: Aeromonas allosaccharophila Strain AE59-TE2 Is Highly Antagonistic towards Multidrug-Resistant Human Pathogens, What Does Its Genome Tell Us?

doi: 10.3390/life12101492

Figure Lengend Snippet: MLSA resultant phylogenetic tree, using six housekeeping genes: recA , gyrB , gltA , metG , groL , and 16S rRNA and 28 genomes from Aeromonas genus, inferred with Maximum Likelihood (ML) method and General Time Reversible model. The model evolutionary used was a discrete Gamma distribution. The bootstrap consensus was inferred from 1000 replicates. Oceanimonas doudoroffi ATCC 27123 was used as an outgroup.

Article Snippet: Oceanimonas doudoroffi ATCC 27123 was used as an outgroup.

Techniques:

Journal:

Article Title: Isolation of Broad-Host-Range Replicons from Marine Sediment Bacteria

doi:

Figure Lengend Snippet: Bacterial strains and plasmids used

Article Snippet: The gels were electrophoresed at 5 V per cm, stained with ethidium bromide, destained in water, and photographed on a UV transilluminator. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Characteristics Reference or source E. coli strains C2110 polAI 27 HB101 recA13 6 DH5α recA1 4 Marine strains Achromobacter sp. Georgia salt marsh isolate, Rif r 52a Pseudomonas nautica ATCC 35188 Rif r ATCC a Pseudomonas duodoroffii ATCC 27123 Rif r ATCC Roseobacter sp. strain 164 San Diego salt marsh isolate, source of repSD164 This study Serratia rubidaea ATCC 27164 Rif r ATCC Vibrio sp. Georgia salt marsh isolate, Rif r 52 Vibrio sp. strain 41 San Diego salt marsh isolate, source of repSD41 This study Vibrio sp. strain 121 San Diego salt marsh isolate, source of repSD121 This study Vibrio sp. strain 172 San Diego salt marsh isolate, source of repSD172 This study Plasmids pUC4K Source of Tn 903 npt gene 59 pRK2013 ColE1 replicon, RK2 Tra + 16 pFF1 Mini-RK2 replicon, bla cat oriT 17 pBR325 pBR322 + Cm r (ColE1) 44 pTM41 pBR325 + repSD41 This study pTM121 pBR325 + repSD121 This study pTM164 pBR325 + repSD164 This study pTM172 pBR325 + repSD172 This study Open in a separate window a ATCC, American Type Culture Collection.

Techniques: Plasmid Preparation

Journal:

Article Title: Isolation of Broad-Host-Range Replicons from Marine Sediment Bacteria

doi:

Figure Lengend Snippet: Schematic diagram of the structural organization of the plasmid replication origins isolated from gram-negative marine sediment bacterial isolates capable of replicating in E. coli, as deduced from the nucleotide sequence. The directions of DnaA boxes are indicated by broad arrows, and the sequences of the plasmid replication origins are similar to the consensus sequence 5′-(T/C)(T/C)(A/T/C)T(A/C)C(A/G)(A/T/C)(A/C)-3′ (49). (A) The 2,327-bp fragment containing the repSD41 replication origin obtained from a 7-kb plasmid isolated from marine Vibrio sp. strain 41. ORF, open reading frame that may encode a replication initiation protein. Direct repeats are indicated by arrowheads. The location of the approximately 100-bp AT-rich region is indicated by the cross-hatched box. (B) The 2,140-bp fragment containing the repSD164 replication origin obtained from a 6.5-kb plasmid isolated from marine Roseobacter sp. strain 164 is diagrammed. The open reading frame (ORF) may encode a replication initiation protein. The locations of a region that potentially is able to form a hairpin structure is indicated by the cross-hatched box. Inverted repeats in close proximity to the repSD164 hairpin structure are indicated by thin arrows. nt, nucleotides. (C) The 1,785-bp fragment containing the repSD172 replication origin obtained from a 30-kb plasmid isolated from marine Vibrio sp. strain 172 is diagrammed. Open reading frames 1 and 2 (ORF 1 and ORF 2) may encode proteins involved in plasmid origin replication. The locations of a region that potentially is able to form a hairpin structure is indicated by the cross-hatched box.

Article Snippet: The gels were electrophoresed at 5 V per cm, stained with ethidium bromide, destained in water, and photographed on a UV transilluminator. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Characteristics Reference or source E. coli strains C2110 polAI 27 HB101 recA13 6 DH5α recA1 4 Marine strains Achromobacter sp. Georgia salt marsh isolate, Rif r 52a Pseudomonas nautica ATCC 35188 Rif r ATCC a Pseudomonas duodoroffii ATCC 27123 Rif r ATCC Roseobacter sp. strain 164 San Diego salt marsh isolate, source of repSD164 This study Serratia rubidaea ATCC 27164 Rif r ATCC Vibrio sp. Georgia salt marsh isolate, Rif r 52 Vibrio sp. strain 41 San Diego salt marsh isolate, source of repSD41 This study Vibrio sp. strain 121 San Diego salt marsh isolate, source of repSD121 This study Vibrio sp. strain 172 San Diego salt marsh isolate, source of repSD172 This study Plasmids pUC4K Source of Tn 903 npt gene 59 pRK2013 ColE1 replicon, RK2 Tra + 16 pFF1 Mini-RK2 replicon, bla cat oriT 17 pBR325 pBR322 + Cm r (ColE1) 44 pTM41 pBR325 + repSD41 This study pTM121 pBR325 + repSD121 This study pTM164 pBR325 + repSD164 This study pTM172 pBR325 + repSD172 This study Open in a separate window a ATCC, American Type Culture Collection.

Techniques: Plasmid Preparation, Isolation, Sequencing

Journal:

Article Title: Isolation of Broad-Host-Range Replicons from Marine Sediment Bacteria

doi:

Figure Lengend Snippet: Host ranges of plasmid replication origins isolated from marine sediment isolates

Article Snippet: The gels were electrophoresed at 5 V per cm, stained with ethidium bromide, destained in water, and photographed on a UV transilluminator. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Characteristics Reference or source E. coli strains C2110 polAI 27 HB101 recA13 6 DH5α recA1 4 Marine strains Achromobacter sp. Georgia salt marsh isolate, Rif r 52a Pseudomonas nautica ATCC 35188 Rif r ATCC a Pseudomonas duodoroffii ATCC 27123 Rif r ATCC Roseobacter sp. strain 164 San Diego salt marsh isolate, source of repSD164 This study Serratia rubidaea ATCC 27164 Rif r ATCC Vibrio sp. Georgia salt marsh isolate, Rif r 52 Vibrio sp. strain 41 San Diego salt marsh isolate, source of repSD41 This study Vibrio sp. strain 121 San Diego salt marsh isolate, source of repSD121 This study Vibrio sp. strain 172 San Diego salt marsh isolate, source of repSD172 This study Plasmids pUC4K Source of Tn 903 npt gene 59 pRK2013 ColE1 replicon, RK2 Tra + 16 pFF1 Mini-RK2 replicon, bla cat oriT 17 pBR325 pBR322 + Cm r (ColE1) 44 pTM41 pBR325 + repSD41 This study pTM121 pBR325 + repSD121 This study pTM164 pBR325 + repSD164 This study pTM172 pBR325 + repSD172 This study Open in a separate window a ATCC, American Type Culture Collection.

Techniques: Plasmid Preparation, Isolation