pp 1 inhibitor okadaic acid sodium salt  (Alomone Labs)

Journal: PLoS ONE

Article Title: Dual Effects of Hydrogen Sulfide Donor on Meiosis and Cumulus Expansion of Porcine Cumulus-Oocyte Complexes

doi: 10.1371/journal.pone.0099613

Figure Lengend Snippet: Proportion of GVBD (A) and meiosis I to II transition (B) in oocytes during in vitro cultivation over 2 h time scale. H 2 S: 300 µM Na 2 S. *Statistically significant differences between control and H 2 S groups (P<0.05).

Article Snippet: The culture medium contained 150, 300, 600 or 900 µM Na 2 S.9H 2 O (Sigma-Aldrich, USA), the H 2 S donor.

Techniques: In Vitro

Journal: PLoS ONE

Article Title: Dual Effects of Hydrogen Sulfide Donor on Meiosis and Cumulus Expansion of Porcine Cumulus-Oocyte Complexes

doi: 10.1371/journal.pone.0099613

Figure Lengend Snippet: Representative autoradiograms and signal quantifications of phosphorylated histone H1 (A) and MBP (B) reflecting MPF and MAPK activity, respectively. Kinase activity was measured in oocytes cultivated with or without Na 2 S over 2 h time scale. The kinase activity was related to oocytes cultivated for 24 hs. C: control; H 2 S: 300 µM Na 2 S. *Statistically significant differences between control and H 2 S groups (P<0.05).

Article Snippet: The culture medium contained 150, 300, 600 or 900 µM Na 2 S.9H 2 O (Sigma-Aldrich, USA), the H 2 S donor.

Techniques: Activity Assay

Journal: PLoS ONE

Article Title: Dual Effects of Hydrogen Sulfide Donor on Meiosis and Cumulus Expansion of Porcine Cumulus-Oocyte Complexes

doi: 10.1371/journal.pone.0099613

Figure Lengend Snippet: Proportion of GVBD (A) and meiosis I to II transition (B) during in vitro cultivation after 20 and 30 h in vitro cultivation, respectively. H 2 S: 300 µM Na 2 S. a,b,c Statistically significant differences among experimental groups (P<0.05).

Article Snippet: The culture medium contained 150, 300, 600 or 900 µM Na 2 S.9H 2 O (Sigma-Aldrich, USA), the H 2 S donor.

Techniques: In Vitro

Journal: PLoS ONE

Article Title: Dual Effects of Hydrogen Sulfide Donor on Meiosis and Cumulus Expansion of Porcine Cumulus-Oocyte Complexes

doi: 10.1371/journal.pone.0099613

Figure Lengend Snippet: (A) Total and retained HA content in COCs cultivated with 150–900 µM Na 2 S for 48 hs, total HA is related to the control group. (B) Total and retained HA content in COCs during in vitro cultivation with 300 µM Na 2 S over 12 h time scale, total HA is related to the control group after 48 h cultivation. (C) Total and retained HA content in COCs and OOXs cultivated with or without H 2 S donor, total HA is related to the control group of COCs. H 2 S: 300 µM Na 2 S. a,b,c Statistically significant differences among experimental groups in total HA, 1,2 statistically significant differences among experimental groups in retained HA, *statistically significant differences in total HA between control and H 2 S groups (P<0.05).

Article Snippet: The culture medium contained 150, 300, 600 or 900 µM Na 2 S.9H 2 O (Sigma-Aldrich, USA), the H 2 S donor.

Techniques: In Vitro

Journal: PLoS ONE

Article Title: Dual Effects of Hydrogen Sulfide Donor on Meiosis and Cumulus Expansion of Porcine Cumulus-Oocyte Complexes

doi: 10.1371/journal.pone.0099613

Figure Lengend Snippet: Effect of Na 2 S on partenogenetic development of porcine oocytes.

Article Snippet: The culture medium contained 150, 300, 600 or 900 µM Na 2 S.9H 2 O (Sigma-Aldrich, USA), the H 2 S donor.

Techniques: