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5 7 dimethoxy 2 4 methoxyphenyl chromen 4  (Alomone Labs)


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    Alomone Labs 5 7 dimethoxy 2 4 methoxyphenyl chromen 4
    5 7 Dimethoxy 2 4 Methoxyphenyl Chromen 4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/o-145/pmc12849215-41-34-37?v=Alomone+Labs
    Average 90 stars, based on 2 article reviews
    5 7 dimethoxy 2 4 methoxyphenyl chromen 4 - by Bioz Stars, 2026-07
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    Over-expression of miR-145 in SW620 cell line affects cell morphology and proliferation . (A) The genomic region surrounding the miR-145 gene was PCR-amplified and cloned into pSilencer 4.1 under control of the CMV promoter. Mature miR-145 was detected by Northern analysis in a pooled population of SW620 cells following transfection. U6 snRNA was used as a loading control. (B) A major distinguishing feature of the cell population over-expressing miR-145 was the change in cell morphology from the round single cells of SW620 to elongated cells with extended processes typical of fibroblast-like cells. (C) The miR-145-expressing SW620 cell population showed a two-fold increase in anchorage-independent growth when grown in the presence of serum and a greater than 50% increase in cell proliferation/metabolic activity when grown in the presence (solid bars) or absence (open bars) of serum. *** p < 0.001. (D) Western analysis of the SW620/miR-145 cells and control cells shows the steady-state levels of E-cadherin were 50% lower in SW620 cells expressing the mature miR-145. FBS = fetal bovine serum, SF = serum-free.

    Journal: BMC Cancer

    Article Title: Characterization of global microRNA expression reveals oncogenic potential of miR-145 in metastatic colorectal cancer

    doi: 10.1186/1471-2407-9-374

    Figure Lengend Snippet: Over-expression of miR-145 in SW620 cell line affects cell morphology and proliferation . (A) The genomic region surrounding the miR-145 gene was PCR-amplified and cloned into pSilencer 4.1 under control of the CMV promoter. Mature miR-145 was detected by Northern analysis in a pooled population of SW620 cells following transfection. U6 snRNA was used as a loading control. (B) A major distinguishing feature of the cell population over-expressing miR-145 was the change in cell morphology from the round single cells of SW620 to elongated cells with extended processes typical of fibroblast-like cells. (C) The miR-145-expressing SW620 cell population showed a two-fold increase in anchorage-independent growth when grown in the presence of serum and a greater than 50% increase in cell proliferation/metabolic activity when grown in the presence (solid bars) or absence (open bars) of serum. *** p < 0.001. (D) Western analysis of the SW620/miR-145 cells and control cells shows the steady-state levels of E-cadherin were 50% lower in SW620 cells expressing the mature miR-145. FBS = fetal bovine serum, SF = serum-free.

    Article Snippet: Biotinylated 2'-O-methyl antisense miR-145 RNA or controls were delivered to SW620 using Lipofectamine 2000 (Invitrogen) as detailed in the Supplementary Methods.

    Techniques: Over Expression, Amplification, Clone Assay, Northern Blot, Transfection, Expressing, Activity Assay, Western Blot

    Antisense-mediated reversion of miR-145-induced proliferation . (A) Total RNA from treated samples showed that miR-145 was depleted in cells receiving the miR-145-specific 2'Ome antisense RNA but not in the mock treated, or miR-145 sense treated controls. (B) As expected, SW620/miR-145 expressing pools showed increased proliferation compared to vector controls in the presence (solid bars) or absence (open bars) of serum. When treated with miR-145 antisense RNA, a reduction in proliferation was seen in both SW620/vector and SW620/miR-145 pools. Three independent experiments were performed. * p < 0.05; ** p < 0.01, *** p < 0.001.

    Journal: BMC Cancer

    Article Title: Characterization of global microRNA expression reveals oncogenic potential of miR-145 in metastatic colorectal cancer

    doi: 10.1186/1471-2407-9-374

    Figure Lengend Snippet: Antisense-mediated reversion of miR-145-induced proliferation . (A) Total RNA from treated samples showed that miR-145 was depleted in cells receiving the miR-145-specific 2'Ome antisense RNA but not in the mock treated, or miR-145 sense treated controls. (B) As expected, SW620/miR-145 expressing pools showed increased proliferation compared to vector controls in the presence (solid bars) or absence (open bars) of serum. When treated with miR-145 antisense RNA, a reduction in proliferation was seen in both SW620/vector and SW620/miR-145 pools. Three independent experiments were performed. * p < 0.05; ** p < 0.01, *** p < 0.001.

    Article Snippet: Biotinylated 2'-O-methyl antisense miR-145 RNA or controls were delivered to SW620 using Lipofectamine 2000 (Invitrogen) as detailed in the Supplementary Methods.

    Techniques: Expressing, Plasmid Preparation