o generuler ultra low range dna ladder  (Thermo Fisher)


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    Name:
    O GeneRuler Ultra Low Range DNA Ladder
    Description:
    Thermo Scientific O GeneRuler Ultra Low Range DNA Ladder 10 300 bp ready to use is recommended for sizing and approximate quantification of double stranded DNA on agarose or polyacrylamide gels It is ideal for denaturing polyacrylamide gel electrophoresis The ladder is a mixture of chromatography purified individual DNA fragments O GeneRuler Ultra Low Range DNA Ladder ready to use is premixed with 6X Orange DNA Loading Dye containing xylene cyanol FF and orange G for direct loading on gel In a 1 agarose gel orange G dye migrates at 50 bp O GeneRuler Ultra Low Range DNA Ladder is ideal when visualization of small DNA fragments is important The ladder is recommended for analysis of siRNA see Figure 1 and is supplied with 6X Orange DNA Loading Dye for sample DNA Highlights• Ideal for both DNA sizing and approximate quantification• Ideal for non denaturing polyacrylamide gel electrophoresis• Ideal for analysis of siRNA• Sharp bands• Bright reference bands• Can be directly loaded and are stable at room temperature for 6 months• Supplied with loading dye for sample DNA
    Catalog Number:
    sm1223
    Price:
    None
    Applications:
    Agarose Gel Electrophoresis|DNA & RNA Purification & Analysis|Nucleic Acid Gel Electrophoresis & Blotting
    Category:
    Standards Ladders Controls
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    Structured Review

    Thermo Fisher o generuler ultra low range dna ladder
    Electrophoretic analysis of partial pep A gene (596 bp) of B. pseudomallei digested with Stu I and Hinc II restriction analysis. (12% polyacrylamide gel, 1X TBE buffer, 8 V/cm, 130 min); Lane M- <t>O’GeneRuler™</t> ultra low range <t>DNA</t> ladder; Lane 1- B. pseudomallei NCTC 13178; Lane 2- B. pseudomallei ATCC 23343; Lane 3- Type I; Lane 4- Type II; Lane 5- Type III.
    Thermo Scientific O GeneRuler Ultra Low Range DNA Ladder 10 300 bp ready to use is recommended for sizing and approximate quantification of double stranded DNA on agarose or polyacrylamide gels It is ideal for denaturing polyacrylamide gel electrophoresis The ladder is a mixture of chromatography purified individual DNA fragments O GeneRuler Ultra Low Range DNA Ladder ready to use is premixed with 6X Orange DNA Loading Dye containing xylene cyanol FF and orange G for direct loading on gel In a 1 agarose gel orange G dye migrates at 50 bp O GeneRuler Ultra Low Range DNA Ladder is ideal when visualization of small DNA fragments is important The ladder is recommended for analysis of siRNA see Figure 1 and is supplied with 6X Orange DNA Loading Dye for sample DNA Highlights• Ideal for both DNA sizing and approximate quantification• Ideal for non denaturing polyacrylamide gel electrophoresis• Ideal for analysis of siRNA• Sharp bands• Bright reference bands• Can be directly loaded and are stable at room temperature for 6 months• Supplied with loading dye for sample DNA
    https://www.bioz.com/result/o generuler ultra low range dna ladder/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    o generuler ultra low range dna ladder - by Bioz Stars, 2020-04
    99/100 stars

    Related Products / Commonly Used Together

    o’generuler 1 kb dna ladder

    Images

    1) Product Images from "Enzymatic and molecular characterisation of leucine aminopeptidase of Burkholderia pseudomallei"

    Article Title: Enzymatic and molecular characterisation of leucine aminopeptidase of Burkholderia pseudomallei

    Journal: BMC Microbiology

    doi: 10.1186/1471-2180-13-110

    Electrophoretic analysis of partial pep A gene (596 bp) of B. pseudomallei digested with Stu I and Hinc II restriction analysis. (12% polyacrylamide gel, 1X TBE buffer, 8 V/cm, 130 min); Lane M- O’GeneRuler™ ultra low range DNA ladder; Lane 1- B. pseudomallei NCTC 13178; Lane 2- B. pseudomallei ATCC 23343; Lane 3- Type I; Lane 4- Type II; Lane 5- Type III.
    Figure Legend Snippet: Electrophoretic analysis of partial pep A gene (596 bp) of B. pseudomallei digested with Stu I and Hinc II restriction analysis. (12% polyacrylamide gel, 1X TBE buffer, 8 V/cm, 130 min); Lane M- O’GeneRuler™ ultra low range DNA ladder; Lane 1- B. pseudomallei NCTC 13178; Lane 2- B. pseudomallei ATCC 23343; Lane 3- Type I; Lane 4- Type II; Lane 5- Type III.

    Techniques Used:

    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Efficiency and specificity of microRNA-primed nucleotide analog incorporation by various DNA polymerases
    Article Snippet: The miRNAs were RNase-free HPLC purified (Integrated DNA Technologies). .. O’GeneRuler™ ultra low range DNA ladder was purchased from Fermentas (Glen Burnie, MD).

    Marker:

    Article Title: Enzymatic and molecular characterisation of leucine aminopeptidase of Burkholderia pseudomallei
    Article Snippet: .. An O’GeneRuler™ Ultra Low Range DNA ladder (Fermentas, Lithuania) was used as molecular weight marker. ..

    Functional Assay:

    Article Title: Putative Mechanisms Underlying High Inhibitory Activities of Bimodular DNA Aptamers to Thrombin
    Article Snippet: O’GeneRuler Ultra Low Range DNA Ladder was purchased from ThermoFisher Scientific (Vilnius, Lithuania). .. The concentrations of cations correspond to the physiological conditions of blood plasma; this buffer is the same as used for functional tests earlier [ ].

    Amplification:

    Article Title: Enzymatic and molecular characterisation of leucine aminopeptidase of Burkholderia pseudomallei
    Article Snippet: The amplified products were purified and subjected to digestion using Stu I followed by Hinc II restriction endonucleases (Fermentas, Lithuania). .. An O’GeneRuler™ Ultra Low Range DNA ladder (Fermentas, Lithuania) was used as molecular weight marker.

    Synthesized:

    Article Title: Putative Mechanisms Underlying High Inhibitory Activities of Bimodular DNA Aptamers to Thrombin
    Article Snippet: Materials and Methods Oligonucleotides were chemically synthesized by Evrogen (Moscow, Russian Federation). .. O’GeneRuler Ultra Low Range DNA Ladder was purchased from ThermoFisher Scientific (Vilnius, Lithuania).

    Multiple Displacement Amplification:

    Article Title: Apolipoprotein E gene polymorphism influences aggressive behavior in prostate cancer cells by deregulating cholesterol homeostasis
    Article Snippet: Materials Human prostatic adenocarcinoma cells LNCaP, PC3, DU145 and MDA PCa 2b were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). .. Gene ruler ultra-low range DNA ladder and Fast digest Hha I restriction enzyme kit was supplied by Fermentas Inc. (Glen Burnie, MD, USA), while Novex TBE running buffer, Hi-density sample buffer and polyacrylamide 8% TBE gels were supplied by Invitrogen (Carlsbad, CA, USA).

    Polymerase Chain Reaction:

    Article Title: Rapid and direct microRNA quantification by an enzymatic luminescence assay
    Article Snippet: E. coli total RNA, human heart total RNA, the TaqMan microRNA reverse transcription kit, TaqMan microRNA forward and reverse primers, and TaqMan universal PCR master mix were purchased from Applied Biosystems (Austin, TX). .. O’GeneRuler™ 1 kb DNA ladder, O’GeneRuler™ ultra low range DNA ladder, and orange loading dye were purchased from Fermentas (Glen Burnie, MD).

    Article Title: Enzymatic and molecular characterisation of leucine aminopeptidase of Burkholderia pseudomallei
    Article Snippet: Partial fragments (596 bp) of pep A gene were amplified from each isolate using primers pepA442-F and pepA1037-R using PCR conditions as described above, except for a higher annealing temperature of 63.9°C. .. An O’GeneRuler™ Ultra Low Range DNA ladder (Fermentas, Lithuania) was used as molecular weight marker.

    Cell Culture:

    Article Title: Apolipoprotein E gene polymorphism influences aggressive behavior in prostate cancer cells by deregulating cholesterol homeostasis
    Article Snippet: Sigma-Aldrich supplied all the cell culture supplements, adenosine 3′,5′ cyclic monophosphate (cAMP), acyl-CoA cholesterol acyltransferase (ACAT) inhibitor (Sandoz 58-035), cholic acid, enhanced avian reverse HS RT-PCR kit, and (2-hydroxypropyl)-β-cyclodextrin. .. Gene ruler ultra-low range DNA ladder and Fast digest Hha I restriction enzyme kit was supplied by Fermentas Inc. (Glen Burnie, MD, USA), while Novex TBE running buffer, Hi-density sample buffer and polyacrylamide 8% TBE gels were supplied by Invitrogen (Carlsbad, CA, USA).

    Purification:

    Article Title: Enzymatic and molecular characterisation of leucine aminopeptidase of Burkholderia pseudomallei
    Article Snippet: The amplified products were purified and subjected to digestion using Stu I followed by Hinc II restriction endonucleases (Fermentas, Lithuania). .. An O’GeneRuler™ Ultra Low Range DNA ladder (Fermentas, Lithuania) was used as molecular weight marker.

    Article Title: Efficiency and specificity of microRNA-primed nucleotide analog incorporation by various DNA polymerases
    Article Snippet: The miRNAs were RNase-free HPLC purified (Integrated DNA Technologies). .. O’GeneRuler™ ultra low range DNA ladder was purchased from Fermentas (Glen Burnie, MD).

    SYBR Green Assay:

    Article Title: Rapid and direct microRNA quantification by an enzymatic luminescence assay
    Article Snippet: SYBR Green II was purchased from Lonza (Rockland, ME). .. O’GeneRuler™ 1 kb DNA ladder, O’GeneRuler™ ultra low range DNA ladder, and orange loading dye were purchased from Fermentas (Glen Burnie, MD).

    Concentration Assay:

    Article Title: Putative Mechanisms Underlying High Inhibitory Activities of Bimodular DNA Aptamers to Thrombin
    Article Snippet: O’GeneRuler Ultra Low Range DNA Ladder was purchased from ThermoFisher Scientific (Vilnius, Lithuania). .. Oligonucleotide samples were prepared in 2 µM concentration in 20 mM Tris-HCl buffer, at pH 7.4, with 140 mM NaCl, 5 mM KCl, 1 mM MgCl2 , and 1 mM CaCl2 .

    other:

    Article Title: Mycobacterium tuberculosis RecG Protein but Not RuvAB or RecA Protein Is Efficient at Remodeling the Stalled Replication Forks
    Article Snippet: T4 polynucleotide kinase, Gene Ruler 50-bp DNA ladder (SM0371), and Gene Ruler Ultra low range DNA ladder (SM01211) were purchased from Thermo Scientific.

    Molecular Weight:

    Article Title: Enzymatic and molecular characterisation of leucine aminopeptidase of Burkholderia pseudomallei
    Article Snippet: .. An O’GeneRuler™ Ultra Low Range DNA ladder (Fermentas, Lithuania) was used as molecular weight marker. ..

    Polyacrylamide Gel Electrophoresis:

    Article Title: Enzymatic and molecular characterisation of leucine aminopeptidase of Burkholderia pseudomallei
    Article Snippet: The RFLP products were analysed by non-denaturing PAGE using 12% polyacrylamide gel and stained with 0.5 μg/ml ethidium bromide. .. An O’GeneRuler™ Ultra Low Range DNA ladder (Fermentas, Lithuania) was used as molecular weight marker.

    Modification:

    Article Title: Efficiency and specificity of microRNA-primed nucleotide analog incorporation by various DNA polymerases
    Article Snippet: The modified nucleoside triphosphate 2′-deoxyadenosine-5′-O-(1-thiotriphosphate) (dATPαS) was purchased from TriLink Biotechnologies (San Diego, CA). .. O’GeneRuler™ ultra low range DNA ladder was purchased from Fermentas (Glen Burnie, MD).

    Staining:

    Article Title: Enzymatic and molecular characterisation of leucine aminopeptidase of Burkholderia pseudomallei
    Article Snippet: The RFLP products were analysed by non-denaturing PAGE using 12% polyacrylamide gel and stained with 0.5 μg/ml ethidium bromide. .. An O’GeneRuler™ Ultra Low Range DNA ladder (Fermentas, Lithuania) was used as molecular weight marker.

    Liquid Chromatography:

    Article Title: Functional Analysis of DNA Replication Fork Reversal Catalyzed by Mycobacterium tuberculosis RuvAB Proteins
    Article Snippet: Gene Ruler 50-bp DNA ladder (SM0371) and Gene Ruler ultra low range DNA ladder (SM01211) were procured from Fermentas Life Sciences. .. Fast performance liquid chromatography columns were purchased from GE Healthcare.

    Plasmid Preparation:

    Article Title: Functional Analysis of DNA Replication Fork Reversal Catalyzed by Mycobacterium tuberculosis RuvAB Proteins
    Article Snippet: Paragraph title: Biochemicals, Plasmid DNA, Oligonucleotides, Bacterial Strains, and Enzymes ... Gene Ruler 50-bp DNA ladder (SM0371) and Gene Ruler ultra low range DNA ladder (SM01211) were procured from Fermentas Life Sciences.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Apolipoprotein E gene polymorphism influences aggressive behavior in prostate cancer cells by deregulating cholesterol homeostasis
    Article Snippet: Sigma-Aldrich supplied all the cell culture supplements, adenosine 3′,5′ cyclic monophosphate (cAMP), acyl-CoA cholesterol acyltransferase (ACAT) inhibitor (Sandoz 58-035), cholic acid, enhanced avian reverse HS RT-PCR kit, and (2-hydroxypropyl)-β-cyclodextrin. .. Gene ruler ultra-low range DNA ladder and Fast digest Hha I restriction enzyme kit was supplied by Fermentas Inc. (Glen Burnie, MD, USA), while Novex TBE running buffer, Hi-density sample buffer and polyacrylamide 8% TBE gels were supplied by Invitrogen (Carlsbad, CA, USA).

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  • 99
    Thermo Fisher o generuler ultra low range dna ladder
    O Generuler Ultra Low Range Dna Ladder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/o generuler ultra low range dna ladder/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    o generuler ultra low range dna ladder - by Bioz Stars, 2020-04
    99/100 stars
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