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Sakura Finetek tissue tek o c t compound
Tissue Tek O C T Compound, supplied by Sakura Finetek, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tissue tek o c t compound/product/Sakura Finetek
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
tissue tek o c t compound - by Bioz Stars, 2021-07
86/100 stars

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Article Title: Efficient Transdermal Delivery of Alendronate, a Nitrogen-Containing Bisphosphonate, Using Tip-Loaded Self-Dissolving Microneedle Arrays for the Treatment of Osteoporosis
Article Snippet: Tissue-Tek® O.C.T. compound was purchased from Sakura Finetek Japan Company, Ltd. (Tokyo, Japan).

Immunofluorescence:

Article Title: Lipopolysaccharide-induced CCN1 production enhances interleukin-6 secretion in bronchial epithelial cells
Article Snippet: Total protein and IL-6 and CCN1 in BALF were measured with an enhanced BCA Protein Assay Kit (P0010S, Beyotime, China) and murine cytokine-specific Quantikine ELISA Kits (R & D Systems). .. Tissue immunofluorescence and immunohistochemistry analysis The right lungs of ALI mice were fixed in 4% paraformaldehyde, infiltrated with 30% sucrose/PBS under the pressure of 20 cmH2 O, embedded in Tissue-Tek OCT compound (Sakura Finetek, Torrance, CA, USA), and fresh-frozen in liquid nitrogen. .. Five-micrometer and 50-μm-thick sections were stored at −80 °C and then fixed in ice-acetone for 10 min. Then, lung tissues were incubated overnight with mouse monoclonal antibodies against EpCAM, CD31 (Abcam, HK, China, 1:200), or CCN1 (Santa Cruz, CA,1:100), respectively, and then incubated with the corresponding secondary antibodies (Abcam, HK, China, 1:100).

Immunohistochemistry:

Article Title: Lipopolysaccharide-induced CCN1 production enhances interleukin-6 secretion in bronchial epithelial cells
Article Snippet: Total protein and IL-6 and CCN1 in BALF were measured with an enhanced BCA Protein Assay Kit (P0010S, Beyotime, China) and murine cytokine-specific Quantikine ELISA Kits (R & D Systems). .. Tissue immunofluorescence and immunohistochemistry analysis The right lungs of ALI mice were fixed in 4% paraformaldehyde, infiltrated with 30% sucrose/PBS under the pressure of 20 cmH2 O, embedded in Tissue-Tek OCT compound (Sakura Finetek, Torrance, CA, USA), and fresh-frozen in liquid nitrogen. .. Five-micrometer and 50-μm-thick sections were stored at −80 °C and then fixed in ice-acetone for 10 min. Then, lung tissues were incubated overnight with mouse monoclonal antibodies against EpCAM, CD31 (Abcam, HK, China, 1:200), or CCN1 (Santa Cruz, CA,1:100), respectively, and then incubated with the corresponding secondary antibodies (Abcam, HK, China, 1:100).

Article Title: Generation and Characterization of Specific Antibodies to the Murine and Human Ectonucleotidase NTPDase8
Article Snippet: In some experiments, a gel with a large sample well 6 cm long containing 120 μg of lysates, transferred and blocked as described above and then probed with antibodies using the Mini-Protean II multiscreen apparatus (Bio-Rad Laboratories Ltd., Mississauga, ON, Canada) in which 20 antibodies can be tested on one gel. .. Immunocytochemistry and Immunohistochemistry Mouse liver was collected after animal perfusion with 4% PFA, tissues were then fixed in 4% PFA for 2 h and incubated O/N in 4% sucrose at 4°C and frozen in Tissue-Tek® O.C.T.TM Compound (Sakura Finetek, Torrance, CA, USA). .. COS-7 cells or tissues sections (6 μm thick) of mouse liver were fixed in 10% phosphate-buffered formalin (Fisher Scientific, Ottawa, ON, Canada) mixed with cold acetone (Fisher Scientific, Ottawa, ON, Canada) and blocked in a PBS solution containing 7% normal goat serum for 30 min. COS-7 cells and tissue sections were incubated with the indicated primary antibody at 4°C.

Mouse Assay:

Article Title: Lipopolysaccharide-induced CCN1 production enhances interleukin-6 secretion in bronchial epithelial cells
Article Snippet: Total protein and IL-6 and CCN1 in BALF were measured with an enhanced BCA Protein Assay Kit (P0010S, Beyotime, China) and murine cytokine-specific Quantikine ELISA Kits (R & D Systems). .. Tissue immunofluorescence and immunohistochemistry analysis The right lungs of ALI mice were fixed in 4% paraformaldehyde, infiltrated with 30% sucrose/PBS under the pressure of 20 cmH2 O, embedded in Tissue-Tek OCT compound (Sakura Finetek, Torrance, CA, USA), and fresh-frozen in liquid nitrogen. .. Five-micrometer and 50-μm-thick sections were stored at −80 °C and then fixed in ice-acetone for 10 min. Then, lung tissues were incubated overnight with mouse monoclonal antibodies against EpCAM, CD31 (Abcam, HK, China, 1:200), or CCN1 (Santa Cruz, CA,1:100), respectively, and then incubated with the corresponding secondary antibodies (Abcam, HK, China, 1:100).

Staining:

Article Title: The characterization of human oral mucosal fibroblasts and their use as feeder cells in cultivated epithelial sheets
Article Snippet: Slides were then rinsed with deionized water, counterstained with hematoxylin (Wako Pure Chemical Industries, Ltd.) for 15 min and observed under a microscope. .. Safranin O stain The induced micromasses were frozen in Tissue-Tek OCT Compound (Sakura Finetek, Tokyo, Japan), and sliced into 5-μm-thick sections. .. The sections were fixed in 10% formalin solution (Wako Pure Chemical Industries, Ltd.) for 10 min.

Immunocytochemistry:

Article Title: Generation and Characterization of Specific Antibodies to the Murine and Human Ectonucleotidase NTPDase8
Article Snippet: In some experiments, a gel with a large sample well 6 cm long containing 120 μg of lysates, transferred and blocked as described above and then probed with antibodies using the Mini-Protean II multiscreen apparatus (Bio-Rad Laboratories Ltd., Mississauga, ON, Canada) in which 20 antibodies can be tested on one gel. .. Immunocytochemistry and Immunohistochemistry Mouse liver was collected after animal perfusion with 4% PFA, tissues were then fixed in 4% PFA for 2 h and incubated O/N in 4% sucrose at 4°C and frozen in Tissue-Tek® O.C.T.TM Compound (Sakura Finetek, Torrance, CA, USA). .. COS-7 cells or tissues sections (6 μm thick) of mouse liver were fixed in 10% phosphate-buffered formalin (Fisher Scientific, Ottawa, ON, Canada) mixed with cold acetone (Fisher Scientific, Ottawa, ON, Canada) and blocked in a PBS solution containing 7% normal goat serum for 30 min. COS-7 cells and tissue sections were incubated with the indicated primary antibody at 4°C.

Incubation:

Article Title: Generation and Characterization of Specific Antibodies to the Murine and Human Ectonucleotidase NTPDase8
Article Snippet: In some experiments, a gel with a large sample well 6 cm long containing 120 μg of lysates, transferred and blocked as described above and then probed with antibodies using the Mini-Protean II multiscreen apparatus (Bio-Rad Laboratories Ltd., Mississauga, ON, Canada) in which 20 antibodies can be tested on one gel. .. Immunocytochemistry and Immunohistochemistry Mouse liver was collected after animal perfusion with 4% PFA, tissues were then fixed in 4% PFA for 2 h and incubated O/N in 4% sucrose at 4°C and frozen in Tissue-Tek® O.C.T.TM Compound (Sakura Finetek, Torrance, CA, USA). .. COS-7 cells or tissues sections (6 μm thick) of mouse liver were fixed in 10% phosphate-buffered formalin (Fisher Scientific, Ottawa, ON, Canada) mixed with cold acetone (Fisher Scientific, Ottawa, ON, Canada) and blocked in a PBS solution containing 7% normal goat serum for 30 min. COS-7 cells and tissue sections were incubated with the indicated primary antibody at 4°C.

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    Sakura Finetek tissue tek o c t compound
    Tissue Tek O C T Compound, supplied by Sakura Finetek, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tissue tek o c t compound/product/Sakura Finetek
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tissue tek o c t compound - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

    86
    Sakura Finetek o c t compound
    O C T Compound, supplied by Sakura Finetek, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/o c t compound/product/Sakura Finetek
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    o c t compound - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

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