nutrient broth no 4  (Millipore)


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    Name:
    Nutrient broth
    Description:

    Catalog Number:
    n7519
    Price:
    None
    Applications:
    Nutrient broth has been used:. for culturing Azospirillum brasilense. for reconstitution of freeze dried Staphylococcus aureus culture. for culturing Pseudomonas aeruginosa NCIMB 8626
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    Structured Review

    Millipore nutrient broth no 4

    https://www.bioz.com/result/nutrient broth no 4/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nutrient broth no 4 - by Bioz Stars, 2020-04
    99/100 stars

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    erlenmeyer flasks
    sporulation medium dsm

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    Related Articles

    Centrifugation:

    Article Title: Isolation of extraintestinal pathogenic Escherichia coli from diarrheic dogs and their antimicrobial resistance profile
    Article Snippet: E. coli isolates were grown overnight in nutrient broth (Sigma Chemical Co, St Louis, USA) at 37°C, and test for the presence of pap, sfa and afa genes using the polymerase chain reaction protocol of Le Bouguenec et al . ( ). .. DNA templates were prepared by pelleting 1 ml of each culture by centrifugation (12000g), resuspension in 250 μl of sterile distilled water and boiling for 10 min. After renewed centrifugation, supernatants were used for PCR in an Eppendorf Mastercycler (Eppendorf AG, Hamburg, Germany).

    Article Title: Quantification and isolation of Bacillus subtilis spores using cell sorting and automated gating
    Article Snippet: .. Cells from the overnight cultures were harvested by centrifugation and inoculated into 500 mL Erlenmeyer flasks containing 200 mL prewarmed Difco sporulation medium (DSM) [8 g/L Nutrient broth No. 4, 1 g/L KCl, 1 mM MgSO4 , 1 mM Ca(NO3 )2 , 10 μM MnCl2 , 1 μM FeSO4 (Sigma-Aldrich, Germany)]. ..

    Amplification:

    Article Title: Isolation of extraintestinal pathogenic Escherichia coli from diarrheic dogs and their antimicrobial resistance profile
    Article Snippet: E. coli isolates were grown overnight in nutrient broth (Sigma Chemical Co, St Louis, USA) at 37°C, and test for the presence of pap, sfa and afa genes using the polymerase chain reaction protocol of Le Bouguenec et al . ( ). .. The amplified DNA products were separated by electrophoresis on 1.5% agarose gel, stained with ethidium bromide and examined under ultraviolet light.

    Positive Control:

    Article Title: Activity of Bacteriophages in Removing Biofilms of Pseudomonas aeruginosa Isolates from Chronic Rhinosinusitis Patients
    Article Snippet: .. Treatments included each of the 4 phages and the phage cocktail in nutrient broth at concentrations of 107 and 108 PFU/mL, as well as equivalent volumes of heat-inactivated stocks of the 4 phages, with PBS as negative control and 2.5% cetylpyridinium chloride (Sigma-Aldrich, St Louis, MO, USA) as a positive control. ..

    Electrophoresis:

    Article Title: Isolation of extraintestinal pathogenic Escherichia coli from diarrheic dogs and their antimicrobial resistance profile
    Article Snippet: E. coli isolates were grown overnight in nutrient broth (Sigma Chemical Co, St Louis, USA) at 37°C, and test for the presence of pap, sfa and afa genes using the polymerase chain reaction protocol of Le Bouguenec et al . ( ). .. The amplified DNA products were separated by electrophoresis on 1.5% agarose gel, stained with ethidium bromide and examined under ultraviolet light.

    Incubation:

    Article Title: Probiotic Potential of Lactobacillus Strains with Antimicrobial Activity against Some Human Pathogenic Strains
    Article Snippet: After colony development, the plates were overlaid with 10 mL of soft (0.8% (w/v) agar) microorganism-specific medium, seeded with 1% (v/v) of an active overnight culture of the target pathogenic strain (final concentration of 7 log CFU/mL), and incubated aerobically at 37°C, except for Candida albicans where the incubation temperature was 24°C. .. The microorganism-specific media were yeast mold broth for Candida albicans (ATCC 44831), reinforced clostridial broth for Propionibacterium acnes (ATCC 6919), trypticase soy broth for Staphylococcus aureus (S244) and Enterococcus faecium (ATCC 51558), brain heart broth for Listeria monocytogenes , and nutrient broth for the other pathogenic strains (all media from Sigma, USA).

    Article Title: Activity of Bacteriophages in Removing Biofilms of Pseudomonas aeruginosa Isolates from Chronic Rhinosinusitis Patients
    Article Snippet: The plate was then incubated for 48 h on a gyratory mixer at 37°C. .. Treatments included each of the 4 phages and the phage cocktail in nutrient broth at concentrations of 107 and 108 PFU/mL, as well as equivalent volumes of heat-inactivated stocks of the 4 phages, with PBS as negative control and 2.5% cetylpyridinium chloride (Sigma-Aldrich, St Louis, MO, USA) as a positive control.

    Article Title: Isolation of extraintestinal pathogenic Escherichia coli from diarrheic dogs and their antimicrobial resistance profile
    Article Snippet: Samples were collected under the supervision of a veterinarian, using a rectal sterile cotton swab, placed in Stuart transport medium, and immediately processed in the laboratory by transfer to MacConkey agar (Mac-Difco Laboratories, Detroit, USA) and incubation for 24 h at 37°C. .. E. coli isolates were grown overnight in nutrient broth (Sigma Chemical Co, St Louis, USA) at 37°C, and test for the presence of pap, sfa and afa genes using the polymerase chain reaction protocol of Le Bouguenec et al . ( ).

    Article Title: Bordetella pseudohinzii targets cilia and impairs tracheal cilia-driven transport in naturally acquired infection in mice
    Article Snippet: Each sample was examined by streaking onto blood agar plate (Oxoid GmbH, Wesel, Germany), on brain heart infusion (BHI) agar (Oxoid GmbH) and selective agar plates (blood agar containing cephalexin hydrate [20 mg/ml; Sigma Aldrich]; nutrient broth no. 2 and agarose containing neomycin trisulphate salt hydrate [2 mg/ml; Sigma Aldrich] and bacitracin [3 mg/ml; Carl Roth, Karlsruhe, Germany]; water-blue metachrome-yellow lactose agar [acc. to Gassner, E. Merck]). .. For enrichment purposes, each sample was inoculated to nutrient broth no. 2 containing 10% bovine serum and broth were streaked onto blood and Gassner agar plate after 24 h incubation at 37 °C.

    Article Title: Combination of Origanum vulgare L. essential oil and lactic acid to inhibit Staphylococcus aureus in meat broth and meat model
    Article Snippet: 5 mL of double strength Nutrient Broth-NB (Sigma, France) was inoculated with 1 mL of the bacterial inocula (approximately 107 cfu.mL -1 ). .. After that, 4 mL of the essential oil or lactic acid solution at appropriate concentration was added, followed by shaking for 30 s, and then incubated for 24 h at 37°C without shaking.

    Article Title: Real-time PCR assay is superior to other methods for the detection of mycoplasma contamination in the cell lines of the National Cell Bank of Iran
    Article Snippet: These cell lines were incubated at 37 °C in 88 % humidified and 5 % CO2 atmosphere. .. Accordingly, Nutrient Broth (Sigma-Aldrich® , FLUKA, USA), Sabouraud Dextrose Broth (BD-Difco, Franklin Lakes, NJ, USA), Thioglycolate Broth (BD-BBL™, USA), Brain Heart Infusion Broth (BD-BBL™, USA), Trypticase Soy Broth (BD-BBL™, USA), Yeast Malt Broth (Sigma-Aldrich® , USA), Blood Agar (Sigma-Aldrich® , FLUKA, USA), Nutrient Agar (BD-Difco™, USA), MacConkey agar (Sigma-Aldrich® , FLUKA, USA), Sabouraud Dextrose Agar (BD-RODAC™, USA) and Brain Heart Infusion agar (BD-BBL™, USA) were used for investigation of microbial contaminants and quality control (Hay and Ikonomi ).

    Diffusion-based Assay:

    Article Title: Effect of Tigecycline on Normal Oropharyngeal and Intestinal Microflora
    Article Snippet: The concentrations of tigecycline in sera, saliva samples, and feces were determined microbiologically using the agar plate diffusion method. .. The test agar medium was prepared with nutrient broth (BBL, Cockeysville, MD) and agarose (Sigma, St. Louis, MO), and the indicator strain was Bacillus cereus ATCC 11778 (American Type Culture Collection, Manassas, VA).

    Activity Assay:

    Article Title: Probiotic Potential of Lactobacillus Strains with Antimicrobial Activity against Some Human Pathogenic Strains
    Article Snippet: Paragraph title: 2.5.1. Antagonistic Activity against Pathogens ... The microorganism-specific media were yeast mold broth for Candida albicans (ATCC 44831), reinforced clostridial broth for Propionibacterium acnes (ATCC 6919), trypticase soy broth for Staphylococcus aureus (S244) and Enterococcus faecium (ATCC 51558), brain heart broth for Listeria monocytogenes , and nutrient broth for the other pathogenic strains (all media from Sigma, USA).

    Sterility:

    Article Title: Activity of Bacteriophages in Removing Biofilms of Pseudomonas aeruginosa Isolates from Chronic Rhinosinusitis Patients
    Article Snippet: Wells adjacent to the edge of the plate were filled with 180μL sterile PBS as a sterility control. .. Treatments included each of the 4 phages and the phage cocktail in nutrient broth at concentrations of 107 and 108 PFU/mL, as well as equivalent volumes of heat-inactivated stocks of the 4 phages, with PBS as negative control and 2.5% cetylpyridinium chloride (Sigma-Aldrich, St Louis, MO, USA) as a positive control.

    Inhibition:

    Article Title: Probiotic Potential of Lactobacillus Strains with Antimicrobial Activity against Some Human Pathogenic Strains
    Article Snippet: The microorganism-specific media were yeast mold broth for Candida albicans (ATCC 44831), reinforced clostridial broth for Propionibacterium acnes (ATCC 6919), trypticase soy broth for Staphylococcus aureus (S244) and Enterococcus faecium (ATCC 51558), brain heart broth for Listeria monocytogenes , and nutrient broth for the other pathogenic strains (all media from Sigma, USA). .. After 48 h of incubation, measurements of inhibition zones around the LAB colonies were taken from the outer edge of the colonies to the outer edge of the clear zones.

    Article Title: Combination of Origanum vulgare L. essential oil and lactic acid to inhibit Staphylococcus aureus in meat broth and meat model
    Article Snippet: 5 mL of double strength Nutrient Broth-NB (Sigma, France) was inoculated with 1 mL of the bacterial inocula (approximately 107 cfu.mL -1 ). .. An aliquot (100 µL) of the tubes with no visible bacterial growth was subcultured on sterile NA Petri dishes at 37°C for 48 h to determine if the inhibition was reversible or permanent.

    Article Title: Effect of Tigecycline on Normal Oropharyngeal and Intestinal Microflora
    Article Snippet: The test agar medium was prepared with nutrient broth (BBL, Cockeysville, MD) and agarose (Sigma, St. Louis, MO), and the indicator strain was Bacillus cereus ATCC 11778 (American Type Culture Collection, Manassas, VA). .. Concentrations of tigecycline were determined relative to the diameters of the inhibition zones caused by the corresponding concentrations from the standard series.

    Cell Culture:

    Article Title: G-CSF regulates hematopoietic stem cell activity, in part, through activation of toll-like receptor signaling
    Article Snippet: .. Stool samples were cultured in Nutrient broth (Sigma) and Brain Heart Infusion (BHI) broth (Sigma) at 37C under aerobic conditions. .. Peripheral Blood and Bone Marrow Analysis Peripheral blood was obtained by retro-orbital venous plexus sampling.

    Article Title: Immunity Profiles of Wild-Type and Recombinant Shiga-Like Toxin-Encoding Bacteriophages and Characterization of Novel Double Lysogens
    Article Snippet: .. Putative STEC lysogens were cultured overnight in 10 ml of nutrient broth (NB) at 37°C, and the cultures were membrane filtered (Millipore; 0.45-μm pore diameter). ..

    Article Title: Phase-Variable Surface Structures Are Required for Infection of Campylobacter jejuni by Bacteriophages
    Article Snippet: C. jejuni was cultured at 42°C on Muller-Hinton (MH) agar (Oxoid, Basingstoke, United Kingdom) supplemented with 5% horse blood (Sigma, Poole, United Kingdom) with 5 μg of trimethoprim/ml under standard microaerophilic conditions (5% O2 , 10% CO2 , 85% N2 ) in a MACS VA500 Variable Atmosphere Work Station (Don Whitley Scientific, Shipley, United Kingdom). .. The media used for the propagation of phage and phage screening were as follows: CBHI, 37 g/liter BHI (Oxoid) supplemented with 1 mmol CaCl2 and 1 mmol MgSO4 ; CBHIO, CBHI-0.7% Select Agar (Sigma); CPTA, 15 g/liter Nutrient Broth #2 (Sigma)-0.7% Select Agar (Sigma) supplemented with 10 mmol CaCl2 and 1 mmol MgSO4 ; NZCYM, 22 g/liter NZCYM Broth (Sigma)-1.2% Select Agar (Sigma); and NZCYO, 22 g/liter NZCYM Broth (Sigma)-0.7% Select Agar (Sigma).

    Article Title: Sensitivity of biochemical test in comparison with other methods for the detection of mycoplasma contamination in human and animal cell lines stored in the National Cell Bank of Iran
    Article Snippet: In order to detect the mycoplasma contamination after harvesting, each cell line was cultured in an antibiotic-free medium for at least 4 days without exchanging the medium (Freshney et al. ). .. Accordingly, Nutrient Broth (Sigma-Aldrich® , Cat No: 70122 FLUKA), Sabouraud Dextrose Broth (BD-Difco, Cat No: 238230, Franklin Lakes, NJ, USA), Thioglycolate Broth (BD-BBL™, Cat No: 297642), Brain Heart Infusion Broth (BD-BBL™, Cat No: 221812), Trypticase Soy Broth (BD-BBL™, Cat No:221715), Yeast Malt Broth (Sigma-Aldrich® , Cat No: Y3752), Blood Agar (Sigma-Aldrich® , Cat No: 70133 FLUKA), Nutrient Agar (BD-Difco™, Cat No: 212000), MacConkey Agar (Sigma-Aldrich® , Cat No: 70143 FLUKA), Sabouraud Dextrose Agar (BD-RODAC™, Cat No: 295872), and Brain Heart Infusion agar (BD-BBL™, Cat No: 211065) were used for investigation of microbial contaminants and quality control (Hay and Ikonomi ).

    Article Title: Real-time PCR assay is superior to other methods for the detection of mycoplasma contamination in the cell lines of the National Cell Bank of Iran
    Article Snippet: In order to detect the mycoplasma contamination after harvesting, each cell line was cultured in an antibiotics-free medium for a week (at least 4–5 days) without exchanging the medium (Phelan , ). .. Accordingly, Nutrient Broth (Sigma-Aldrich® , FLUKA, USA), Sabouraud Dextrose Broth (BD-Difco, Franklin Lakes, NJ, USA), Thioglycolate Broth (BD-BBL™, USA), Brain Heart Infusion Broth (BD-BBL™, USA), Trypticase Soy Broth (BD-BBL™, USA), Yeast Malt Broth (Sigma-Aldrich® , USA), Blood Agar (Sigma-Aldrich® , FLUKA, USA), Nutrient Agar (BD-Difco™, USA), MacConkey agar (Sigma-Aldrich® , FLUKA, USA), Sabouraud Dextrose Agar (BD-RODAC™, USA) and Brain Heart Infusion agar (BD-BBL™, USA) were used for investigation of microbial contaminants and quality control (Hay and Ikonomi ).

    Purification:

    Article Title: Quantification and isolation of Bacillus subtilis spores using cell sorting and automated gating
    Article Snippet: Cells from the overnight cultures were harvested by centrifugation and inoculated into 500 mL Erlenmeyer flasks containing 200 mL prewarmed Difco sporulation medium (DSM) [8 g/L Nutrient broth No. 4, 1 g/L KCl, 1 mM MgSO4 , 1 mM Ca(NO3 )2 , 10 μM MnCl2 , 1 μM FeSO4 (Sigma-Aldrich, Germany)]. .. For spore purification, after 72 h of cultivation the cultures were harvested by centrifugation at 3,400 x g for 15 min.

    other:

    Article Title: Influence of carvacrol and thymol on the physiological attributes, enterotoxin production and surface characteristics of Staphylococcus aureus strains isolated from foods
    Article Snippet: Stock solutions were prepared in Nutrient Broth - NB (Sigma, France) using bacteriological agar (0.15 g/100 mL) as a stabilizing agent ( ).

    Article Title: A Synthetic Community Approach Reveals Plant Genotypes Affecting the Phyllosphere Microbiota
    Article Snippet: All other strains were grown on nutrient broth (NB) without additional NaCl (Sigma-Aldrich, St. Louis, MO, USA).

    Polymerase Chain Reaction:

    Article Title: Isolation of extraintestinal pathogenic Escherichia coli from diarrheic dogs and their antimicrobial resistance profile
    Article Snippet: .. E. coli isolates were grown overnight in nutrient broth (Sigma Chemical Co, St Louis, USA) at 37°C, and test for the presence of pap, sfa and afa genes using the polymerase chain reaction protocol of Le Bouguenec et al . ( ). .. DNA templates were prepared by pelleting 1 ml of each culture by centrifugation (12000g), resuspension in 250 μl of sterile distilled water and boiling for 10 min. After renewed centrifugation, supernatants were used for PCR in an Eppendorf Mastercycler (Eppendorf AG, Hamburg, Germany).

    Injection:

    Article Title: G-CSF regulates hematopoietic stem cell activity, in part, through activation of toll-like receptor signaling
    Article Snippet: G-CSF and Antibiotic Administration Mice were injected subcutaneously with twice-daily doses of 125µg/kg of recombinant human G-CSF (Amgen, Thousand Oaks, CA) in low-endotoxin PBS with 0.1% BSA (or vehicle alone). .. Stool samples were cultured in Nutrient broth (Sigma) and Brain Heart Infusion (BHI) broth (Sigma) at 37C under aerobic conditions.

    Recombinant:

    Article Title: G-CSF regulates hematopoietic stem cell activity, in part, through activation of toll-like receptor signaling
    Article Snippet: G-CSF and Antibiotic Administration Mice were injected subcutaneously with twice-daily doses of 125µg/kg of recombinant human G-CSF (Amgen, Thousand Oaks, CA) in low-endotoxin PBS with 0.1% BSA (or vehicle alone). .. Stool samples were cultured in Nutrient broth (Sigma) and Brain Heart Infusion (BHI) broth (Sigma) at 37C under aerobic conditions.

    Magnetic Cell Separation:

    Article Title: Phase-Variable Surface Structures Are Required for Infection of Campylobacter jejuni by Bacteriophages
    Article Snippet: C. jejuni was cultured at 42°C on Muller-Hinton (MH) agar (Oxoid, Basingstoke, United Kingdom) supplemented with 5% horse blood (Sigma, Poole, United Kingdom) with 5 μg of trimethoprim/ml under standard microaerophilic conditions (5% O2 , 10% CO2 , 85% N2 ) in a MACS VA500 Variable Atmosphere Work Station (Don Whitley Scientific, Shipley, United Kingdom). .. The media used for the propagation of phage and phage screening were as follows: CBHI, 37 g/liter BHI (Oxoid) supplemented with 1 mmol CaCl2 and 1 mmol MgSO4 ; CBHIO, CBHI-0.7% Select Agar (Sigma); CPTA, 15 g/liter Nutrient Broth #2 (Sigma)-0.7% Select Agar (Sigma) supplemented with 10 mmol CaCl2 and 1 mmol MgSO4 ; NZCYM, 22 g/liter NZCYM Broth (Sigma)-1.2% Select Agar (Sigma); and NZCYO, 22 g/liter NZCYM Broth (Sigma)-0.7% Select Agar (Sigma).

    Fluorescence:

    Article Title: Sensitivity of biochemical test in comparison with other methods for the detection of mycoplasma contamination in human and animal cell lines stored in the National Cell Bank of Iran
    Article Snippet: Slides were mounted with glycerol and examined under a fluorescence microscope with 340/380 nm excitation filter. .. Accordingly, Nutrient Broth (Sigma-Aldrich® , Cat No: 70122 FLUKA), Sabouraud Dextrose Broth (BD-Difco, Cat No: 238230, Franklin Lakes, NJ, USA), Thioglycolate Broth (BD-BBL™, Cat No: 297642), Brain Heart Infusion Broth (BD-BBL™, Cat No: 221812), Trypticase Soy Broth (BD-BBL™, Cat No:221715), Yeast Malt Broth (Sigma-Aldrich® , Cat No: Y3752), Blood Agar (Sigma-Aldrich® , Cat No: 70133 FLUKA), Nutrient Agar (BD-Difco™, Cat No: 212000), MacConkey Agar (Sigma-Aldrich® , Cat No: 70143 FLUKA), Sabouraud Dextrose Agar (BD-RODAC™, Cat No: 295872), and Brain Heart Infusion agar (BD-BBL™, Cat No: 211065) were used for investigation of microbial contaminants and quality control (Hay and Ikonomi ).

    Isolation:

    Article Title: Immunity Profiles of Wild-Type and Recombinant Shiga-Like Toxin-Encoding Bacteriophages and Characterization of Novel Double Lysogens
    Article Snippet: Paragraph title: Isolation of phage particles from STEC lysogens. ... Putative STEC lysogens were cultured overnight in 10 ml of nutrient broth (NB) at 37°C, and the cultures were membrane filtered (Millipore; 0.45-μm pore diameter).

    Article Title: Isolation of extraintestinal pathogenic Escherichia coli from diarrheic dogs and their antimicrobial resistance profile
    Article Snippet: At least 5 colonies, isolated from each plate, were submitted to further analysis by standard methods for E. coli identification ( ). .. E. coli isolates were grown overnight in nutrient broth (Sigma Chemical Co, St Louis, USA) at 37°C, and test for the presence of pap, sfa and afa genes using the polymerase chain reaction protocol of Le Bouguenec et al . ( ).

    Microscopy:

    Article Title: Sensitivity of biochemical test in comparison with other methods for the detection of mycoplasma contamination in human and animal cell lines stored in the National Cell Bank of Iran
    Article Snippet: Slides were mounted with glycerol and examined under a fluorescence microscope with 340/380 nm excitation filter. .. Accordingly, Nutrient Broth (Sigma-Aldrich® , Cat No: 70122 FLUKA), Sabouraud Dextrose Broth (BD-Difco, Cat No: 238230, Franklin Lakes, NJ, USA), Thioglycolate Broth (BD-BBL™, Cat No: 297642), Brain Heart Infusion Broth (BD-BBL™, Cat No: 221812), Trypticase Soy Broth (BD-BBL™, Cat No:221715), Yeast Malt Broth (Sigma-Aldrich® , Cat No: Y3752), Blood Agar (Sigma-Aldrich® , Cat No: 70133 FLUKA), Nutrient Agar (BD-Difco™, Cat No: 212000), MacConkey Agar (Sigma-Aldrich® , Cat No: 70143 FLUKA), Sabouraud Dextrose Agar (BD-RODAC™, Cat No: 295872), and Brain Heart Infusion agar (BD-BBL™, Cat No: 211065) were used for investigation of microbial contaminants and quality control (Hay and Ikonomi ).

    Titration:

    Article Title: Activity of Bacteriophages in Removing Biofilms of Pseudomonas aeruginosa Isolates from Chronic Rhinosinusitis Patients
    Article Snippet: Treatments included each of the 4 phages and the phage cocktail in nutrient broth at concentrations of 107 and 108 PFU/mL, as well as equivalent volumes of heat-inactivated stocks of the 4 phages, with PBS as negative control and 2.5% cetylpyridinium chloride (Sigma-Aldrich, St Louis, MO, USA) as a positive control. .. Forty-eight hours treatment plates had 50 μL/well nutrient broth replenished at 24 h. At the designated time point (either 24 or 48 h after treatment), liquid contents of the wells were transferred into a fresh plate for post-treatment phage titration.

    Staining:

    Article Title: Activity of Bacteriophages in Removing Biofilms of Pseudomonas aeruginosa Isolates from Chronic Rhinosinusitis Patients
    Article Snippet: Treatments included each of the 4 phages and the phage cocktail in nutrient broth at concentrations of 107 and 108 PFU/mL, as well as equivalent volumes of heat-inactivated stocks of the 4 phages, with PBS as negative control and 2.5% cetylpyridinium chloride (Sigma-Aldrich, St Louis, MO, USA) as a positive control. .. The biofilm plates were gently washed twice with sterile PBS, and then stained with 190 μL/well 0.5% crystal violet (Sigma, St Louis, MO, USA) for 30 min.

    Article Title: Isolation of extraintestinal pathogenic Escherichia coli from diarrheic dogs and their antimicrobial resistance profile
    Article Snippet: E. coli isolates were grown overnight in nutrient broth (Sigma Chemical Co, St Louis, USA) at 37°C, and test for the presence of pap, sfa and afa genes using the polymerase chain reaction protocol of Le Bouguenec et al . ( ). .. The amplified DNA products were separated by electrophoresis on 1.5% agarose gel, stained with ethidium bromide and examined under ultraviolet light.

    Mouse Assay:

    Article Title: G-CSF regulates hematopoietic stem cell activity, in part, through activation of toll-like receptor signaling
    Article Snippet: For antibiotic treatment, mice were given Ciprofloxacin 100mg/L (Sigma), Polymyxin B 70mg/L (Sigma) and 20g/L sucrose (Sigma) in their drinking water. .. Stool samples were cultured in Nutrient broth (Sigma) and Brain Heart Infusion (BHI) broth (Sigma) at 37C under aerobic conditions.

    Article Title: Bordetella pseudohinzii targets cilia and impairs tracheal cilia-driven transport in naturally acquired infection in mice
    Article Snippet: Bacteriological investigations To examine bacterial colonization, the mice were dissected under sterile conditions and samples were taken from larynx, trachea and lung, around 20–40 mg each. .. Each sample was examined by streaking onto blood agar plate (Oxoid GmbH, Wesel, Germany), on brain heart infusion (BHI) agar (Oxoid GmbH) and selective agar plates (blood agar containing cephalexin hydrate [20 mg/ml; Sigma Aldrich]; nutrient broth no. 2 and agarose containing neomycin trisulphate salt hydrate [2 mg/ml; Sigma Aldrich] and bacitracin [3 mg/ml; Carl Roth, Karlsruhe, Germany]; water-blue metachrome-yellow lactose agar [acc. to Gassner, E. Merck]).

    Spot Test:

    Article Title: Probiotic Potential of Lactobacillus Strains with Antimicrobial Activity against Some Human Pathogenic Strains
    Article Snippet: The antagonistic activities of the LAB strains against the 12 pathogenic test strains were evaluated using the agar spot test described by Touré et al. [ ] with modifications. .. The microorganism-specific media were yeast mold broth for Candida albicans (ATCC 44831), reinforced clostridial broth for Propionibacterium acnes (ATCC 6919), trypticase soy broth for Staphylococcus aureus (S244) and Enterococcus faecium (ATCC 51558), brain heart broth for Listeria monocytogenes , and nutrient broth for the other pathogenic strains (all media from Sigma, USA).

    Negative Control:

    Article Title: Activity of Bacteriophages in Removing Biofilms of Pseudomonas aeruginosa Isolates from Chronic Rhinosinusitis Patients
    Article Snippet: .. Treatments included each of the 4 phages and the phage cocktail in nutrient broth at concentrations of 107 and 108 PFU/mL, as well as equivalent volumes of heat-inactivated stocks of the 4 phages, with PBS as negative control and 2.5% cetylpyridinium chloride (Sigma-Aldrich, St Louis, MO, USA) as a positive control. ..

    Selection:

    Article Title: Phase-Variable Surface Structures Are Required for Infection of Campylobacter jejuni by Bacteriophages
    Article Snippet: The media used for the propagation of phage and phage screening were as follows: CBHI, 37 g/liter BHI (Oxoid) supplemented with 1 mmol CaCl2 and 1 mmol MgSO4 ; CBHIO, CBHI-0.7% Select Agar (Sigma); CPTA, 15 g/liter Nutrient Broth #2 (Sigma)-0.7% Select Agar (Sigma) supplemented with 10 mmol CaCl2 and 1 mmol MgSO4 ; NZCYM, 22 g/liter NZCYM Broth (Sigma)-1.2% Select Agar (Sigma); and NZCYO, 22 g/liter NZCYM Broth (Sigma)-0.7% Select Agar (Sigma). .. Where necessary for selection, media were supplemented with chloramphenicol (10 μg/ml), ampicillin (100 μg/ml), or kanamycin (25 μg/ml) as appropriate.

    Agarose Gel Electrophoresis:

    Article Title: Isolation of extraintestinal pathogenic Escherichia coli from diarrheic dogs and their antimicrobial resistance profile
    Article Snippet: E. coli isolates were grown overnight in nutrient broth (Sigma Chemical Co, St Louis, USA) at 37°C, and test for the presence of pap, sfa and afa genes using the polymerase chain reaction protocol of Le Bouguenec et al . ( ). .. The amplified DNA products were separated by electrophoresis on 1.5% agarose gel, stained with ethidium bromide and examined under ultraviolet light.

    Concentration Assay:

    Article Title: Probiotic Potential of Lactobacillus Strains with Antimicrobial Activity against Some Human Pathogenic Strains
    Article Snippet: After colony development, the plates were overlaid with 10 mL of soft (0.8% (w/v) agar) microorganism-specific medium, seeded with 1% (v/v) of an active overnight culture of the target pathogenic strain (final concentration of 7 log CFU/mL), and incubated aerobically at 37°C, except for Candida albicans where the incubation temperature was 24°C. .. The microorganism-specific media were yeast mold broth for Candida albicans (ATCC 44831), reinforced clostridial broth for Propionibacterium acnes (ATCC 6919), trypticase soy broth for Staphylococcus aureus (S244) and Enterococcus faecium (ATCC 51558), brain heart broth for Listeria monocytogenes , and nutrient broth for the other pathogenic strains (all media from Sigma, USA).

    Article Title: Enhanced Fermentative Hydrogen and Methane Production from an Inhibitory Fruit-Flavored Medium with Membrane-Encapsulated Cells
    Article Snippet: Estimation of Chemical Oxygen Demand of the Substrate The substrate used for the fermentation process was a synthetic medium consisting of 20 g/L glucose (supplied by Merck, Millipore, Darmstadt, Germany), 20 g/L yeast extract (supplied by Merck, Millipore, Darmstadt, Germany) and 20 g/L nutrient broth (supplied by Sigma-Aldrich, Stockholm, Sweden). .. The concentration of the substrate carbon source was, therefore, assumed to be 60 g/L., such that the amount of glucose in 1000 mL of the nutrient medium was equivalent to 60g.

    Article Title: Combination of Origanum vulgare L. essential oil and lactic acid to inhibit Staphylococcus aureus in meat broth and meat model
    Article Snippet: Paragraph title: Determination of the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) ... 5 mL of double strength Nutrient Broth-NB (Sigma, France) was inoculated with 1 mL of the bacterial inocula (approximately 107 cfu.mL -1 ).

    Biofilm Production Assay:

    Article Title: Activity of Bacteriophages in Removing Biofilms of Pseudomonas aeruginosa Isolates from Chronic Rhinosinusitis Patients
    Article Snippet: Paragraph title: Biofilm assay ... Treatments included each of the 4 phages and the phage cocktail in nutrient broth at concentrations of 107 and 108 PFU/mL, as well as equivalent volumes of heat-inactivated stocks of the 4 phages, with PBS as negative control and 2.5% cetylpyridinium chloride (Sigma-Aldrich, St Louis, MO, USA) as a positive control.

    Lysis:

    Article Title: Immunity Profiles of Wild-Type and Recombinant Shiga-Like Toxin-Encoding Bacteriophages and Characterization of Novel Double Lysogens
    Article Snippet: Putative STEC lysogens were cultured overnight in 10 ml of nutrient broth (NB) at 37°C, and the cultures were membrane filtered (Millipore; 0.45-μm pore diameter). .. Where indicated, UV induction of lysis was used to enhance the recovery of phage ( ).

    Gradient Centrifugation:

    Article Title: Quantification and isolation of Bacillus subtilis spores using cell sorting and automated gating
    Article Snippet: Cells from the overnight cultures were harvested by centrifugation and inoculated into 500 mL Erlenmeyer flasks containing 200 mL prewarmed Difco sporulation medium (DSM) [8 g/L Nutrient broth No. 4, 1 g/L KCl, 1 mM MgSO4 , 1 mM Ca(NO3 )2 , 10 μM MnCl2 , 1 μM FeSO4 (Sigma-Aldrich, Germany)]. .. The spores were purified using a Renografin gradient centrifugation as described by Nicholson et al. [ ] followed by resuspension in 1 x phosphate buffered saline (PBS).

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    Millipore ãŽâ¼m pes syringe filters
    ãŽâ¼m Pes Syringe Filters, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 1 article reviews
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    ãŽâ¼m pes syringe filters - by Bioz Stars, 2020-04
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