nupl1 nup58  (Proteintech)

Journal: Nucleic Acids Research

Article Title: Nucleoporin 54 contributes to homologous recombination repair and post-replicative DNA integrity

doi: 10.1093/nar/gky569

Figure Lengend Snippet: Identification of central channel Nucleoporins Nup54, Nup62 and Nup58 as novel radioprotecting factors. ( A ) Mean R-scores values for the genes in the secondary screen ordered by rank product. A negative R-score denotes radiosensitization. ( B – E ) CFAs performed in HeLa, T24, BT549, MCF7 and DU145 cells following siRNA transfection with the indicated siRNAs. P -values < 0.05 were considered significant (ANOVA; n = 3 technical replicates from a representative experiment repeated at least three times). Nup54 knockdown was confirmed by western blotting 72 h after transfection (β-actin level was used as a loading control).

Article Snippet: Rabbit polyclonal anti-Nup54 and Nup58 (NupL1) antibodies and mouse monoclonal anti-β-actin antibody were purchased from SIGMA.

Techniques: Transfection, Western Blot

Journal: Nucleic Acids Research

Article Title: Nucleoporin 54 contributes to homologous recombination repair and post-replicative DNA integrity

doi: 10.1093/nar/gky569

Figure Lengend Snippet: Depletion of the central channel nucleoporins Nup54, Nup62 or Nup58 does not affect the nuclear import of 53BP1. ( A ) Fluorescence microscopy images of unirradiated HeLa cells 72 h after transfection with indicated siRNAs, showing 53BP1 (green) and DNA (DAPI, blue) staining. The arrow indicates perinuclear 53BP1 accumulation. ( B ) Quantification of 53BP1 nuclear levels (mean fluorescence intensity). Values shown in graph correspond to mean ± sd (* P < 0.001, two-sided t -test; n = 3 technical replicas from a representative experiment repeated three times).

Article Snippet: Rabbit polyclonal anti-Nup54 and Nup58 (NupL1) antibodies and mouse monoclonal anti-β-actin antibody were purchased from SIGMA.

Techniques: Fluorescence, Microscopy, Transfection, Staining