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Thermo Fisher nup50
Nup50, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nup50/product/Thermo Fisher
Average 84 stars, based on 2 article reviews
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nup50 - by Bioz Stars, 2020-02
84/100 stars

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Related Articles

Clone Assay:

Article Title: Two distinct sites in Nup153 mediate interaction with the SUMO proteases SENP1 and SENP2
Article Snippet: .. Stable transgenic clones were then maintained in media containing 200 μg/mL Geneticin. siRNA depletion of SENP1, SENP2, Nup153, and Nup50 . siRNA oligos against control, SENP1, , SENP2, , Nup153, TPR, and Nup50 were transfected into Hela cells (in 10 nM final concentration) for 48 h using Lipofectamine RNAi MAX (Invitrogen) as previously described in Mackay et al. .. Scrambled siRNA oligo used in is a scrambled sequence based on the Nup153 siRNA oligo.

Transfection:

Article Title: Two distinct sites in Nup153 mediate interaction with the SUMO proteases SENP1 and SENP2
Article Snippet: .. Stable transgenic clones were then maintained in media containing 200 μg/mL Geneticin. siRNA depletion of SENP1, SENP2, Nup153, and Nup50 . siRNA oligos against control, SENP1, , SENP2, , Nup153, TPR, and Nup50 were transfected into Hela cells (in 10 nM final concentration) for 48 h using Lipofectamine RNAi MAX (Invitrogen) as previously described in Mackay et al. .. Scrambled siRNA oligo used in is a scrambled sequence based on the Nup153 siRNA oligo.

Transgenic Assay:

Article Title: Two distinct sites in Nup153 mediate interaction with the SUMO proteases SENP1 and SENP2
Article Snippet: .. Stable transgenic clones were then maintained in media containing 200 μg/mL Geneticin. siRNA depletion of SENP1, SENP2, Nup153, and Nup50 . siRNA oligos against control, SENP1, , SENP2, , Nup153, TPR, and Nup50 were transfected into Hela cells (in 10 nM final concentration) for 48 h using Lipofectamine RNAi MAX (Invitrogen) as previously described in Mackay et al. .. Scrambled siRNA oligo used in is a scrambled sequence based on the Nup153 siRNA oligo.

Isolation:

Article Title: Two distinct sites in Nup153 mediate interaction with the SUMO proteases SENP1 and SENP2
Article Snippet: Geneticin resistant clones were isolated and screened for uniform expression of T7-tagged SUMO1/SUMO3 using indirect immunofluorescence and western analysis. .. Stable transgenic clones were then maintained in media containing 200 μg/mL Geneticin. siRNA depletion of SENP1, SENP2, Nup153, and Nup50 . siRNA oligos against control, SENP1, , SENP2, , Nup153, TPR, and Nup50 were transfected into Hela cells (in 10 nM final concentration) for 48 h using Lipofectamine RNAi MAX (Invitrogen) as previously described in Mackay et al.

Construct:

Article Title: Two distinct sites in Nup153 mediate interaction with the SUMO proteases SENP1 and SENP2
Article Snippet: For generation of stable lines, T7-tagged SUMO1 and SUMO3 expressing constructs were transfected into Hela cells. .. Stable transgenic clones were then maintained in media containing 200 μg/mL Geneticin. siRNA depletion of SENP1, SENP2, Nup153, and Nup50 . siRNA oligos against control, SENP1, , SENP2, , Nup153, TPR, and Nup50 were transfected into Hela cells (in 10 nM final concentration) for 48 h using Lipofectamine RNAi MAX (Invitrogen) as previously described in Mackay et al.

Concentration Assay:

Article Title: Two distinct sites in Nup153 mediate interaction with the SUMO proteases SENP1 and SENP2
Article Snippet: .. Stable transgenic clones were then maintained in media containing 200 μg/mL Geneticin. siRNA depletion of SENP1, SENP2, Nup153, and Nup50 . siRNA oligos against control, SENP1, , SENP2, , Nup153, TPR, and Nup50 were transfected into Hela cells (in 10 nM final concentration) for 48 h using Lipofectamine RNAi MAX (Invitrogen) as previously described in Mackay et al. .. Scrambled siRNA oligo used in is a scrambled sequence based on the Nup153 siRNA oligo.

Expressing:

Article Title: Two distinct sites in Nup153 mediate interaction with the SUMO proteases SENP1 and SENP2
Article Snippet: Paragraph title: Plasmid transfection and generation of stable Hela cell lines expressing T7-tagged SUMO1/SUMO3 ... Stable transgenic clones were then maintained in media containing 200 μg/mL Geneticin. siRNA depletion of SENP1, SENP2, Nup153, and Nup50 . siRNA oligos against control, SENP1, , SENP2, , Nup153, TPR, and Nup50 were transfected into Hela cells (in 10 nM final concentration) for 48 h using Lipofectamine RNAi MAX (Invitrogen) as previously described in Mackay et al.

Sequencing:

Article Title: Two distinct sites in Nup153 mediate interaction with the SUMO proteases SENP1 and SENP2
Article Snippet: Stable transgenic clones were then maintained in media containing 200 μg/mL Geneticin. siRNA depletion of SENP1, SENP2, Nup153, and Nup50 . siRNA oligos against control, SENP1, , SENP2, , Nup153, TPR, and Nup50 were transfected into Hela cells (in 10 nM final concentration) for 48 h using Lipofectamine RNAi MAX (Invitrogen) as previously described in Mackay et al. .. Scrambled siRNA oligo used in is a scrambled sequence based on the Nup153 siRNA oligo.

Western Blot:

Article Title: Two distinct sites in Nup153 mediate interaction with the SUMO proteases SENP1 and SENP2
Article Snippet: Geneticin resistant clones were isolated and screened for uniform expression of T7-tagged SUMO1/SUMO3 using indirect immunofluorescence and western analysis. .. Stable transgenic clones were then maintained in media containing 200 μg/mL Geneticin. siRNA depletion of SENP1, SENP2, Nup153, and Nup50 . siRNA oligos against control, SENP1, , SENP2, , Nup153, TPR, and Nup50 were transfected into Hela cells (in 10 nM final concentration) for 48 h using Lipofectamine RNAi MAX (Invitrogen) as previously described in Mackay et al.

Immunofluorescence:

Article Title: Two distinct sites in Nup153 mediate interaction with the SUMO proteases SENP1 and SENP2
Article Snippet: Geneticin resistant clones were isolated and screened for uniform expression of T7-tagged SUMO1/SUMO3 using indirect immunofluorescence and western analysis. .. Stable transgenic clones were then maintained in media containing 200 μg/mL Geneticin. siRNA depletion of SENP1, SENP2, Nup153, and Nup50 . siRNA oligos against control, SENP1, , SENP2, , Nup153, TPR, and Nup50 were transfected into Hela cells (in 10 nM final concentration) for 48 h using Lipofectamine RNAi MAX (Invitrogen) as previously described in Mackay et al.

Plasmid Preparation:

Article Title: Two distinct sites in Nup153 mediate interaction with the SUMO proteases SENP1 and SENP2
Article Snippet: Paragraph title: Plasmid transfection and generation of stable Hela cell lines expressing T7-tagged SUMO1/SUMO3 ... Stable transgenic clones were then maintained in media containing 200 μg/mL Geneticin. siRNA depletion of SENP1, SENP2, Nup153, and Nup50 . siRNA oligos against control, SENP1, , SENP2, , Nup153, TPR, and Nup50 were transfected into Hela cells (in 10 nM final concentration) for 48 h using Lipofectamine RNAi MAX (Invitrogen) as previously described in Mackay et al.

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