nunc tripleflask treated cell culture flasks  (Thermo Fisher)


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    Name:
    Nunc TripleFlask Treated Cell Culture Flasks
    Description:
    Perform cell culture expansion and experience 500cm2 total growth area within the footprint of a standard flask using Thermo Scientific Nunc TripleFlask Treated Cell Culture Flasks Total culture area is achieved by way of the three parallel layers and a straight neck design that works effectively within automation systems TripleFlask receives the Nunclon Delta treatment a proprietary cell culture surface treatment that offers maximum adhesion for a broad range of cell types
    Catalog Number:
    132865
    Price:
    None
    Applications:
    Cell Culture|Cell Culture Plastics
    Category:
    Lab Supplies Plastics Glassware
    Buy from Supplier


    Structured Review

    Thermo Fisher nunc tripleflask treated cell culture flasks
    Perform cell culture expansion and experience 500cm2 total growth area within the footprint of a standard flask using Thermo Scientific Nunc TripleFlask Treated Cell Culture Flasks Total culture area is achieved by way of the three parallel layers and a straight neck design that works effectively within automation systems TripleFlask receives the Nunclon Delta treatment a proprietary cell culture surface treatment that offers maximum adhesion for a broad range of cell types
    https://www.bioz.com/result/nunc tripleflask treated cell culture flasks/product/Thermo Fisher
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    nunc tripleflask treated cell culture flasks - by Bioz Stars, 2020-09
    94/100 stars

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    Related Articles

    Electron Microscopy:

    Article Title: Loading of Extracellular Vesicles with Chemically Stabilized Hydrophobic siRNAs for the Treatment of Disease in the Central Nervous System
    Article Snippet: .. Tips (from 0.2 μl to 1,000 μl) (VWR) Paper towel Serological pipettes individually wrapped (from 5 ml to 50 ml) (Olympus) 0.22-μm filter-sterilization system (Thermo Fisher Scientific, Thermo Scientific™, catalog number: 567-0020) Tissue culture treated multilayer flask–T500 cm2 triple flask (Thermo Fisher Scientific, Thermo Scientific™, catalog number: 132867) 50 ml conical centrifuge tubes (Thermo Fisher Scientific, Thermo Scientific™, catalog number: 339652) Vacuum-connected Pasteur pipette 1.7 ml microcentrifuge tubes (Genesee Scientific, catalog number: 22-282) Aluminum foil UV-transparent, flat-bottom 96-well plate (Corning, catalog number: 3635) 1 ml syringe (BD, catalog number: 309659) Parafilm (Bemis, Parafilm M® ) Whatman No. 1 filter paper (GE Healthcare, Whatman, catalog number: 10010155) 30 G ½ needle Electron microscopy grids (Electron Microscopy Sciences, catalog number: FCF2010-Ni) and clean forceps to manipulate the grid Grid storage box (Electron Microscopy Sciences, catalog number: 71156) Mouse (FVB/NJ) (THE JACKSON LABORATORY, catalog number: 001800) 200-proof ethanol (Decon Labs, catalog number: 2805M) Fetal bovine serum (FBS) (Mediatech, catalog number: 35-010-CV) Phosphate-buffered saline (PBS) (Thermo Fisher Scientific, Gibco™, catalog number: 14190250) Protease inhibitor cocktail stock solution (Sigma-Aldrich, catalog number: P8340) Cy3-labeled or biotinylated cholesterol-conjugated hsiRNAs (produced in-house) Cy3-OO-PNA strands, fully complementary to the hsiRNA guide strand (20 nucleotides long) (PNA Bio) Peptide-nucleic acid (PNA) hybridization assay (developed by Axolabs, Kulmbach, Germany) RIPA buffer (Thermo Fisher Scientific, Thermo Scientific™, catalog number: 89900) Proteinase K (Thermo Fisher Scientific, Invitrogen™, catalog number: 25530049) Glycine (Sigma-Aldrich, catalog number: G7126) Bovine serum albumin (BSA) (Sigma-Aldrich, catalog number: A2153) Saponin (Sigma-Aldrich, catalog number: S1252) Note: This product has been discontinued. .. Glutaraldehyde (Polysciences, catalog number: 01909-10) Avertin (Sigma-Aldrich, catalog number: T48402) QuantiGene 2.0 Assay Kit (Thermo Fisher Scientific, Invitrogen™, catalog number: QS0011) Quantigene 2.0 Probesets (Varies by gene) Dulbecco’s modified Eagle medium (DMEM) MSC culture media (ATCC, catalog number: PCS-500-041) Sucrose (Sigma-Aldrich, catalog number: S0389) Tris base (TRIZMA) (Sigma-Aldrich, catalog number: T6066) Hydrochloric acid (HCl) (Sigma-Aldrich, catalog number: H1758) Ethylenediaminetetraacetate acid disodium salt (EDTA) (Sigma-Aldrich, catalog number: E6758) Sodium hydroxide (NaOH) (~50 ml of NaOH) (Sigma-Aldrich, catalog number: 72068) Potassium chloride (KCl) (Sigma-Aldrich, catalog number: P9541) Sodium dodecyl sulfate (SDS) (Sigma-Aldrich, catalog number: L3771) Sodium phosphate monobasic monohydrate (NaH2 PO4 .H2 O) Sodium phosphate monobasic (NaH2 PO4 ) (Sigma-Aldrich, catalog number: S3139) Acetonitrile (50% acetonitrile solution, diluted in distilled H2 O) (Fisher Scientific, catalog number: A998-4) Sodium perchlorate monohydrate (NaClO4 .xH2 O) (Fisher Scientific, catalog number: S490-500) Methyl cellulose (Sigma-Aldrich, catalog number: M6385) PFA powder (Sigma-Aldrich, catalog number: P6148) Uranyl acetate (Electron Microscopy Sciences, catalog number: 22400) Oxalic acid Tris-HCl (pH 8.5) (Fisher Scientific, catalog number: BP153) Ammonium hydroxide (NH4 OH) (Fisher Scientific, catalog number: A669-212) Cell culture medium (see Recipes) 1 M sucrose (see Recipes) 1 M Tris-HCl (see Recipes) 0.5 M EDTA (see Recipes) 3 M KCl (see Recipes) 10% SDS (see Recipes) 0.1 M sodium phosphate buffer (see Recipes) HPLC buffer A (see Recipes) HPLC buffer B (see Recipes) Glutaraldehyde, 1% (v/v) (see Recipes) Methyl cellulose, 2% (w/v) (see Recipes) Paraformaldehyde (PFA), 2% and 4% (w/v) (see Recipes) Uranyl acetate (4% w/v), pH 4 (see Recipes) Uranyl-oxalate, pH 7 (see Recipes) Methyl cellulose-UA, pH 4 (see Recipes)

    Transfection:

    Article Title: Fibulin-3, -4, and -5 Are Highly Susceptible to Proteolysis, Interact with Cells and Heparin, and Form Multimers *
    Article Snippet: .. Stably transfected HEK293 cells were plated on eight triple layer flasks (500-cm2 surface area; Nalge Nunc International, product 132913) in DMEM supplemented with 10% FCS, 100 μg/ml penicillin and streptomycin, 2 m m glutamine, and 250 μg/ml G418 to maintain clonal selection. ..

    Selection:

    Article Title: Fibulin-3, -4, and -5 Are Highly Susceptible to Proteolysis, Interact with Cells and Heparin, and Form Multimers *
    Article Snippet: .. Stably transfected HEK293 cells were plated on eight triple layer flasks (500-cm2 surface area; Nalge Nunc International, product 132913) in DMEM supplemented with 10% FCS, 100 μg/ml penicillin and streptomycin, 2 m m glutamine, and 250 μg/ml G418 to maintain clonal selection. ..

    Stable Transfection:

    Article Title: Fibulin-3, -4, and -5 Are Highly Susceptible to Proteolysis, Interact with Cells and Heparin, and Form Multimers *
    Article Snippet: .. Stably transfected HEK293 cells were plated on eight triple layer flasks (500-cm2 surface area; Nalge Nunc International, product 132913) in DMEM supplemented with 10% FCS, 100 μg/ml penicillin and streptomycin, 2 m m glutamine, and 250 μg/ml G418 to maintain clonal selection. ..

    Protease Inhibitor:

    Article Title: Loading of Extracellular Vesicles with Chemically Stabilized Hydrophobic siRNAs for the Treatment of Disease in the Central Nervous System
    Article Snippet: .. Tips (from 0.2 μl to 1,000 μl) (VWR) Paper towel Serological pipettes individually wrapped (from 5 ml to 50 ml) (Olympus) 0.22-μm filter-sterilization system (Thermo Fisher Scientific, Thermo Scientific™, catalog number: 567-0020) Tissue culture treated multilayer flask–T500 cm2 triple flask (Thermo Fisher Scientific, Thermo Scientific™, catalog number: 132867) 50 ml conical centrifuge tubes (Thermo Fisher Scientific, Thermo Scientific™, catalog number: 339652) Vacuum-connected Pasteur pipette 1.7 ml microcentrifuge tubes (Genesee Scientific, catalog number: 22-282) Aluminum foil UV-transparent, flat-bottom 96-well plate (Corning, catalog number: 3635) 1 ml syringe (BD, catalog number: 309659) Parafilm (Bemis, Parafilm M® ) Whatman No. 1 filter paper (GE Healthcare, Whatman, catalog number: 10010155) 30 G ½ needle Electron microscopy grids (Electron Microscopy Sciences, catalog number: FCF2010-Ni) and clean forceps to manipulate the grid Grid storage box (Electron Microscopy Sciences, catalog number: 71156) Mouse (FVB/NJ) (THE JACKSON LABORATORY, catalog number: 001800) 200-proof ethanol (Decon Labs, catalog number: 2805M) Fetal bovine serum (FBS) (Mediatech, catalog number: 35-010-CV) Phosphate-buffered saline (PBS) (Thermo Fisher Scientific, Gibco™, catalog number: 14190250) Protease inhibitor cocktail stock solution (Sigma-Aldrich, catalog number: P8340) Cy3-labeled or biotinylated cholesterol-conjugated hsiRNAs (produced in-house) Cy3-OO-PNA strands, fully complementary to the hsiRNA guide strand (20 nucleotides long) (PNA Bio) Peptide-nucleic acid (PNA) hybridization assay (developed by Axolabs, Kulmbach, Germany) RIPA buffer (Thermo Fisher Scientific, Thermo Scientific™, catalog number: 89900) Proteinase K (Thermo Fisher Scientific, Invitrogen™, catalog number: 25530049) Glycine (Sigma-Aldrich, catalog number: G7126) Bovine serum albumin (BSA) (Sigma-Aldrich, catalog number: A2153) Saponin (Sigma-Aldrich, catalog number: S1252) Note: This product has been discontinued. .. Glutaraldehyde (Polysciences, catalog number: 01909-10) Avertin (Sigma-Aldrich, catalog number: T48402) QuantiGene 2.0 Assay Kit (Thermo Fisher Scientific, Invitrogen™, catalog number: QS0011) Quantigene 2.0 Probesets (Varies by gene) Dulbecco’s modified Eagle medium (DMEM) MSC culture media (ATCC, catalog number: PCS-500-041) Sucrose (Sigma-Aldrich, catalog number: S0389) Tris base (TRIZMA) (Sigma-Aldrich, catalog number: T6066) Hydrochloric acid (HCl) (Sigma-Aldrich, catalog number: H1758) Ethylenediaminetetraacetate acid disodium salt (EDTA) (Sigma-Aldrich, catalog number: E6758) Sodium hydroxide (NaOH) (~50 ml of NaOH) (Sigma-Aldrich, catalog number: 72068) Potassium chloride (KCl) (Sigma-Aldrich, catalog number: P9541) Sodium dodecyl sulfate (SDS) (Sigma-Aldrich, catalog number: L3771) Sodium phosphate monobasic monohydrate (NaH2 PO4 .H2 O) Sodium phosphate monobasic (NaH2 PO4 ) (Sigma-Aldrich, catalog number: S3139) Acetonitrile (50% acetonitrile solution, diluted in distilled H2 O) (Fisher Scientific, catalog number: A998-4) Sodium perchlorate monohydrate (NaClO4 .xH2 O) (Fisher Scientific, catalog number: S490-500) Methyl cellulose (Sigma-Aldrich, catalog number: M6385) PFA powder (Sigma-Aldrich, catalog number: P6148) Uranyl acetate (Electron Microscopy Sciences, catalog number: 22400) Oxalic acid Tris-HCl (pH 8.5) (Fisher Scientific, catalog number: BP153) Ammonium hydroxide (NH4 OH) (Fisher Scientific, catalog number: A669-212) Cell culture medium (see Recipes) 1 M sucrose (see Recipes) 1 M Tris-HCl (see Recipes) 0.5 M EDTA (see Recipes) 3 M KCl (see Recipes) 10% SDS (see Recipes) 0.1 M sodium phosphate buffer (see Recipes) HPLC buffer A (see Recipes) HPLC buffer B (see Recipes) Glutaraldehyde, 1% (v/v) (see Recipes) Methyl cellulose, 2% (w/v) (see Recipes) Paraformaldehyde (PFA), 2% and 4% (w/v) (see Recipes) Uranyl acetate (4% w/v), pH 4 (see Recipes) Uranyl-oxalate, pH 7 (see Recipes) Methyl cellulose-UA, pH 4 (see Recipes)

    Transferring:

    Article Title: Measurement of TLR4 and CD14 Receptor Endocytosis Using Flow Cytometry
    Article Snippet: .. Pipette tips 5 ml round, disposable round-bottom tube (FACS Tube) (Corning, Falcon® , catalog number: 352052) Aluminum foil (Genesee Scientific, catalog number: 88-101) 0.2 μm bottle filter (Thermo Fisher Scientific, Nalgene™, catalog number: 566-0020) 6-well non-treated culture plates (Corning, catalog number: 3736) Sterile cell scrapers (Fisher Scientific, Fisherbrand™, catalog number: 08-100-240) Sterile individually packaged serological pipette (10 ml) (Greiner Bio One International, catalog number: 607160) Sterile individually packaged serological pipette (5 ml) (Greiner Bio One International, catalog number: 606160) 1.7 ml microfuge Eppendorf tubes (Genesee Scientific, Olympus Plastics, catalog number: 24-281) Nunc™ TripleFlask™ Treated Cell Culture Flasks (Thermo Fisher Scientific, catalog number: 132867) Falcon® 50 ml Conical Centrifuge Tube (Corning, catalog number: 352098) ..

    Article Title: Loading of Extracellular Vesicles with Chemically Stabilized Hydrophobic siRNAs for the Treatment of Disease in the Central Nervous System
    Article Snippet: .. Tips (from 0.2 μl to 1,000 μl) (VWR) Paper towel Serological pipettes individually wrapped (from 5 ml to 50 ml) (Olympus) 0.22-μm filter-sterilization system (Thermo Fisher Scientific, Thermo Scientific™, catalog number: 567-0020) Tissue culture treated multilayer flask–T500 cm2 triple flask (Thermo Fisher Scientific, Thermo Scientific™, catalog number: 132867) 50 ml conical centrifuge tubes (Thermo Fisher Scientific, Thermo Scientific™, catalog number: 339652) Vacuum-connected Pasteur pipette 1.7 ml microcentrifuge tubes (Genesee Scientific, catalog number: 22-282) Aluminum foil UV-transparent, flat-bottom 96-well plate (Corning, catalog number: 3635) 1 ml syringe (BD, catalog number: 309659) Parafilm (Bemis, Parafilm M® ) Whatman No. 1 filter paper (GE Healthcare, Whatman, catalog number: 10010155) 30 G ½ needle Electron microscopy grids (Electron Microscopy Sciences, catalog number: FCF2010-Ni) and clean forceps to manipulate the grid Grid storage box (Electron Microscopy Sciences, catalog number: 71156) Mouse (FVB/NJ) (THE JACKSON LABORATORY, catalog number: 001800) 200-proof ethanol (Decon Labs, catalog number: 2805M) Fetal bovine serum (FBS) (Mediatech, catalog number: 35-010-CV) Phosphate-buffered saline (PBS) (Thermo Fisher Scientific, Gibco™, catalog number: 14190250) Protease inhibitor cocktail stock solution (Sigma-Aldrich, catalog number: P8340) Cy3-labeled or biotinylated cholesterol-conjugated hsiRNAs (produced in-house) Cy3-OO-PNA strands, fully complementary to the hsiRNA guide strand (20 nucleotides long) (PNA Bio) Peptide-nucleic acid (PNA) hybridization assay (developed by Axolabs, Kulmbach, Germany) RIPA buffer (Thermo Fisher Scientific, Thermo Scientific™, catalog number: 89900) Proteinase K (Thermo Fisher Scientific, Invitrogen™, catalog number: 25530049) Glycine (Sigma-Aldrich, catalog number: G7126) Bovine serum albumin (BSA) (Sigma-Aldrich, catalog number: A2153) Saponin (Sigma-Aldrich, catalog number: S1252) Note: This product has been discontinued. .. Glutaraldehyde (Polysciences, catalog number: 01909-10) Avertin (Sigma-Aldrich, catalog number: T48402) QuantiGene 2.0 Assay Kit (Thermo Fisher Scientific, Invitrogen™, catalog number: QS0011) Quantigene 2.0 Probesets (Varies by gene) Dulbecco’s modified Eagle medium (DMEM) MSC culture media (ATCC, catalog number: PCS-500-041) Sucrose (Sigma-Aldrich, catalog number: S0389) Tris base (TRIZMA) (Sigma-Aldrich, catalog number: T6066) Hydrochloric acid (HCl) (Sigma-Aldrich, catalog number: H1758) Ethylenediaminetetraacetate acid disodium salt (EDTA) (Sigma-Aldrich, catalog number: E6758) Sodium hydroxide (NaOH) (~50 ml of NaOH) (Sigma-Aldrich, catalog number: 72068) Potassium chloride (KCl) (Sigma-Aldrich, catalog number: P9541) Sodium dodecyl sulfate (SDS) (Sigma-Aldrich, catalog number: L3771) Sodium phosphate monobasic monohydrate (NaH2 PO4 .H2 O) Sodium phosphate monobasic (NaH2 PO4 ) (Sigma-Aldrich, catalog number: S3139) Acetonitrile (50% acetonitrile solution, diluted in distilled H2 O) (Fisher Scientific, catalog number: A998-4) Sodium perchlorate monohydrate (NaClO4 .xH2 O) (Fisher Scientific, catalog number: S490-500) Methyl cellulose (Sigma-Aldrich, catalog number: M6385) PFA powder (Sigma-Aldrich, catalog number: P6148) Uranyl acetate (Electron Microscopy Sciences, catalog number: 22400) Oxalic acid Tris-HCl (pH 8.5) (Fisher Scientific, catalog number: BP153) Ammonium hydroxide (NH4 OH) (Fisher Scientific, catalog number: A669-212) Cell culture medium (see Recipes) 1 M sucrose (see Recipes) 1 M Tris-HCl (see Recipes) 0.5 M EDTA (see Recipes) 3 M KCl (see Recipes) 10% SDS (see Recipes) 0.1 M sodium phosphate buffer (see Recipes) HPLC buffer A (see Recipes) HPLC buffer B (see Recipes) Glutaraldehyde, 1% (v/v) (see Recipes) Methyl cellulose, 2% (w/v) (see Recipes) Paraformaldehyde (PFA), 2% and 4% (w/v) (see Recipes) Uranyl acetate (4% w/v), pH 4 (see Recipes) Uranyl-oxalate, pH 7 (see Recipes) Methyl cellulose-UA, pH 4 (see Recipes)

    Cell Culture:

    Article Title: Measurement of TLR4 and CD14 Receptor Endocytosis Using Flow Cytometry
    Article Snippet: .. Pipette tips 5 ml round, disposable round-bottom tube (FACS Tube) (Corning, Falcon® , catalog number: 352052) Aluminum foil (Genesee Scientific, catalog number: 88-101) 0.2 μm bottle filter (Thermo Fisher Scientific, Nalgene™, catalog number: 566-0020) 6-well non-treated culture plates (Corning, catalog number: 3736) Sterile cell scrapers (Fisher Scientific, Fisherbrand™, catalog number: 08-100-240) Sterile individually packaged serological pipette (10 ml) (Greiner Bio One International, catalog number: 607160) Sterile individually packaged serological pipette (5 ml) (Greiner Bio One International, catalog number: 606160) 1.7 ml microfuge Eppendorf tubes (Genesee Scientific, Olympus Plastics, catalog number: 24-281) Nunc™ TripleFlask™ Treated Cell Culture Flasks (Thermo Fisher Scientific, catalog number: 132867) Falcon® 50 ml Conical Centrifuge Tube (Corning, catalog number: 352098) ..

    Produced:

    Article Title: Loading of Extracellular Vesicles with Chemically Stabilized Hydrophobic siRNAs for the Treatment of Disease in the Central Nervous System
    Article Snippet: .. Tips (from 0.2 μl to 1,000 μl) (VWR) Paper towel Serological pipettes individually wrapped (from 5 ml to 50 ml) (Olympus) 0.22-μm filter-sterilization system (Thermo Fisher Scientific, Thermo Scientific™, catalog number: 567-0020) Tissue culture treated multilayer flask–T500 cm2 triple flask (Thermo Fisher Scientific, Thermo Scientific™, catalog number: 132867) 50 ml conical centrifuge tubes (Thermo Fisher Scientific, Thermo Scientific™, catalog number: 339652) Vacuum-connected Pasteur pipette 1.7 ml microcentrifuge tubes (Genesee Scientific, catalog number: 22-282) Aluminum foil UV-transparent, flat-bottom 96-well plate (Corning, catalog number: 3635) 1 ml syringe (BD, catalog number: 309659) Parafilm (Bemis, Parafilm M® ) Whatman No. 1 filter paper (GE Healthcare, Whatman, catalog number: 10010155) 30 G ½ needle Electron microscopy grids (Electron Microscopy Sciences, catalog number: FCF2010-Ni) and clean forceps to manipulate the grid Grid storage box (Electron Microscopy Sciences, catalog number: 71156) Mouse (FVB/NJ) (THE JACKSON LABORATORY, catalog number: 001800) 200-proof ethanol (Decon Labs, catalog number: 2805M) Fetal bovine serum (FBS) (Mediatech, catalog number: 35-010-CV) Phosphate-buffered saline (PBS) (Thermo Fisher Scientific, Gibco™, catalog number: 14190250) Protease inhibitor cocktail stock solution (Sigma-Aldrich, catalog number: P8340) Cy3-labeled or biotinylated cholesterol-conjugated hsiRNAs (produced in-house) Cy3-OO-PNA strands, fully complementary to the hsiRNA guide strand (20 nucleotides long) (PNA Bio) Peptide-nucleic acid (PNA) hybridization assay (developed by Axolabs, Kulmbach, Germany) RIPA buffer (Thermo Fisher Scientific, Thermo Scientific™, catalog number: 89900) Proteinase K (Thermo Fisher Scientific, Invitrogen™, catalog number: 25530049) Glycine (Sigma-Aldrich, catalog number: G7126) Bovine serum albumin (BSA) (Sigma-Aldrich, catalog number: A2153) Saponin (Sigma-Aldrich, catalog number: S1252) Note: This product has been discontinued. .. Glutaraldehyde (Polysciences, catalog number: 01909-10) Avertin (Sigma-Aldrich, catalog number: T48402) QuantiGene 2.0 Assay Kit (Thermo Fisher Scientific, Invitrogen™, catalog number: QS0011) Quantigene 2.0 Probesets (Varies by gene) Dulbecco’s modified Eagle medium (DMEM) MSC culture media (ATCC, catalog number: PCS-500-041) Sucrose (Sigma-Aldrich, catalog number: S0389) Tris base (TRIZMA) (Sigma-Aldrich, catalog number: T6066) Hydrochloric acid (HCl) (Sigma-Aldrich, catalog number: H1758) Ethylenediaminetetraacetate acid disodium salt (EDTA) (Sigma-Aldrich, catalog number: E6758) Sodium hydroxide (NaOH) (~50 ml of NaOH) (Sigma-Aldrich, catalog number: 72068) Potassium chloride (KCl) (Sigma-Aldrich, catalog number: P9541) Sodium dodecyl sulfate (SDS) (Sigma-Aldrich, catalog number: L3771) Sodium phosphate monobasic monohydrate (NaH2 PO4 .H2 O) Sodium phosphate monobasic (NaH2 PO4 ) (Sigma-Aldrich, catalog number: S3139) Acetonitrile (50% acetonitrile solution, diluted in distilled H2 O) (Fisher Scientific, catalog number: A998-4) Sodium perchlorate monohydrate (NaClO4 .xH2 O) (Fisher Scientific, catalog number: S490-500) Methyl cellulose (Sigma-Aldrich, catalog number: M6385) PFA powder (Sigma-Aldrich, catalog number: P6148) Uranyl acetate (Electron Microscopy Sciences, catalog number: 22400) Oxalic acid Tris-HCl (pH 8.5) (Fisher Scientific, catalog number: BP153) Ammonium hydroxide (NH4 OH) (Fisher Scientific, catalog number: A669-212) Cell culture medium (see Recipes) 1 M sucrose (see Recipes) 1 M Tris-HCl (see Recipes) 0.5 M EDTA (see Recipes) 3 M KCl (see Recipes) 10% SDS (see Recipes) 0.1 M sodium phosphate buffer (see Recipes) HPLC buffer A (see Recipes) HPLC buffer B (see Recipes) Glutaraldehyde, 1% (v/v) (see Recipes) Methyl cellulose, 2% (w/v) (see Recipes) Paraformaldehyde (PFA), 2% and 4% (w/v) (see Recipes) Uranyl acetate (4% w/v), pH 4 (see Recipes) Uranyl-oxalate, pH 7 (see Recipes) Methyl cellulose-UA, pH 4 (see Recipes)

    Isolation:

    Article Title: The Rab32/BLOC-3 dependent pathway mediates host- defence against different pathogens in human macrophages
    Article Snippet: .. Isolated peripheral blood mononuclear cells were resuspended in DMEM containing 10% autologous human serum (freshly prepared from the same donor) and seeded on coverslips or tissue-treated plastic. .. Cells were plated at 5×105 /well in 24-well plates.

    FACS:

    Article Title: Measurement of TLR4 and CD14 Receptor Endocytosis Using Flow Cytometry
    Article Snippet: .. Pipette tips 5 ml round, disposable round-bottom tube (FACS Tube) (Corning, Falcon® , catalog number: 352052) Aluminum foil (Genesee Scientific, catalog number: 88-101) 0.2 μm bottle filter (Thermo Fisher Scientific, Nalgene™, catalog number: 566-0020) 6-well non-treated culture plates (Corning, catalog number: 3736) Sterile cell scrapers (Fisher Scientific, Fisherbrand™, catalog number: 08-100-240) Sterile individually packaged serological pipette (10 ml) (Greiner Bio One International, catalog number: 607160) Sterile individually packaged serological pipette (5 ml) (Greiner Bio One International, catalog number: 606160) 1.7 ml microfuge Eppendorf tubes (Genesee Scientific, Olympus Plastics, catalog number: 24-281) Nunc™ TripleFlask™ Treated Cell Culture Flasks (Thermo Fisher Scientific, catalog number: 132867) Falcon® 50 ml Conical Centrifuge Tube (Corning, catalog number: 352098) ..

    Hybridization:

    Article Title: Loading of Extracellular Vesicles with Chemically Stabilized Hydrophobic siRNAs for the Treatment of Disease in the Central Nervous System
    Article Snippet: .. Tips (from 0.2 μl to 1,000 μl) (VWR) Paper towel Serological pipettes individually wrapped (from 5 ml to 50 ml) (Olympus) 0.22-μm filter-sterilization system (Thermo Fisher Scientific, Thermo Scientific™, catalog number: 567-0020) Tissue culture treated multilayer flask–T500 cm2 triple flask (Thermo Fisher Scientific, Thermo Scientific™, catalog number: 132867) 50 ml conical centrifuge tubes (Thermo Fisher Scientific, Thermo Scientific™, catalog number: 339652) Vacuum-connected Pasteur pipette 1.7 ml microcentrifuge tubes (Genesee Scientific, catalog number: 22-282) Aluminum foil UV-transparent, flat-bottom 96-well plate (Corning, catalog number: 3635) 1 ml syringe (BD, catalog number: 309659) Parafilm (Bemis, Parafilm M® ) Whatman No. 1 filter paper (GE Healthcare, Whatman, catalog number: 10010155) 30 G ½ needle Electron microscopy grids (Electron Microscopy Sciences, catalog number: FCF2010-Ni) and clean forceps to manipulate the grid Grid storage box (Electron Microscopy Sciences, catalog number: 71156) Mouse (FVB/NJ) (THE JACKSON LABORATORY, catalog number: 001800) 200-proof ethanol (Decon Labs, catalog number: 2805M) Fetal bovine serum (FBS) (Mediatech, catalog number: 35-010-CV) Phosphate-buffered saline (PBS) (Thermo Fisher Scientific, Gibco™, catalog number: 14190250) Protease inhibitor cocktail stock solution (Sigma-Aldrich, catalog number: P8340) Cy3-labeled or biotinylated cholesterol-conjugated hsiRNAs (produced in-house) Cy3-OO-PNA strands, fully complementary to the hsiRNA guide strand (20 nucleotides long) (PNA Bio) Peptide-nucleic acid (PNA) hybridization assay (developed by Axolabs, Kulmbach, Germany) RIPA buffer (Thermo Fisher Scientific, Thermo Scientific™, catalog number: 89900) Proteinase K (Thermo Fisher Scientific, Invitrogen™, catalog number: 25530049) Glycine (Sigma-Aldrich, catalog number: G7126) Bovine serum albumin (BSA) (Sigma-Aldrich, catalog number: A2153) Saponin (Sigma-Aldrich, catalog number: S1252) Note: This product has been discontinued. .. Glutaraldehyde (Polysciences, catalog number: 01909-10) Avertin (Sigma-Aldrich, catalog number: T48402) QuantiGene 2.0 Assay Kit (Thermo Fisher Scientific, Invitrogen™, catalog number: QS0011) Quantigene 2.0 Probesets (Varies by gene) Dulbecco’s modified Eagle medium (DMEM) MSC culture media (ATCC, catalog number: PCS-500-041) Sucrose (Sigma-Aldrich, catalog number: S0389) Tris base (TRIZMA) (Sigma-Aldrich, catalog number: T6066) Hydrochloric acid (HCl) (Sigma-Aldrich, catalog number: H1758) Ethylenediaminetetraacetate acid disodium salt (EDTA) (Sigma-Aldrich, catalog number: E6758) Sodium hydroxide (NaOH) (~50 ml of NaOH) (Sigma-Aldrich, catalog number: 72068) Potassium chloride (KCl) (Sigma-Aldrich, catalog number: P9541) Sodium dodecyl sulfate (SDS) (Sigma-Aldrich, catalog number: L3771) Sodium phosphate monobasic monohydrate (NaH2 PO4 .H2 O) Sodium phosphate monobasic (NaH2 PO4 ) (Sigma-Aldrich, catalog number: S3139) Acetonitrile (50% acetonitrile solution, diluted in distilled H2 O) (Fisher Scientific, catalog number: A998-4) Sodium perchlorate monohydrate (NaClO4 .xH2 O) (Fisher Scientific, catalog number: S490-500) Methyl cellulose (Sigma-Aldrich, catalog number: M6385) PFA powder (Sigma-Aldrich, catalog number: P6148) Uranyl acetate (Electron Microscopy Sciences, catalog number: 22400) Oxalic acid Tris-HCl (pH 8.5) (Fisher Scientific, catalog number: BP153) Ammonium hydroxide (NH4 OH) (Fisher Scientific, catalog number: A669-212) Cell culture medium (see Recipes) 1 M sucrose (see Recipes) 1 M Tris-HCl (see Recipes) 0.5 M EDTA (see Recipes) 3 M KCl (see Recipes) 10% SDS (see Recipes) 0.1 M sodium phosphate buffer (see Recipes) HPLC buffer A (see Recipes) HPLC buffer B (see Recipes) Glutaraldehyde, 1% (v/v) (see Recipes) Methyl cellulose, 2% (w/v) (see Recipes) Paraformaldehyde (PFA), 2% and 4% (w/v) (see Recipes) Uranyl acetate (4% w/v), pH 4 (see Recipes) Uranyl-oxalate, pH 7 (see Recipes) Methyl cellulose-UA, pH 4 (see Recipes)

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  • 94
    Thermo Fisher nunc tripleflask treated cell culture flasks
    Nunc Tripleflask Treated Cell Culture Flasks, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nunc tripleflask treated cell culture flasks/product/Thermo Fisher
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    nunc tripleflask treated cell culture flasks - by Bioz Stars, 2020-09
    94/100 stars
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