nunc microwell 96 well optical bottom plates  (Thermo Fisher)


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    Nunc MicroWell 96 Well Optical Bottom Plates
    Description:
    Achieve optimal results in cell based fluorescence and luminescence applications using Thermo Scientific Nunc 96 Well Optical Bottom Plate with Polymer Base featuring a polystyrene black or white upper structure with a polystyrene film at the bottom of the plate Available in cell culture treated non treated collagen I coated and poly D lysine coated surfaces
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    152028
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    Structured Review

    Thermo Fisher nunc microwell 96 well optical bottom plates
    Achieve optimal results in cell based fluorescence and luminescence applications using Thermo Scientific Nunc 96 Well Optical Bottom Plate with Polymer Base featuring a polystyrene black or white upper structure with a polystyrene film at the bottom of the plate Available in cell culture treated non treated collagen I coated and poly D lysine coated surfaces
    https://www.bioz.com/result/nunc microwell 96 well optical bottom plates/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nunc microwell 96 well optical bottom plates - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Transduction:

    Article Title: Generation of a caged lentiviral vector through an unnatural amino acid for photo-switchable transduction
    Article Snippet: .. Transduction and integration of RGD/T1-modified lentiviral vectors To determine in vitro transduction behavior of the RGD/T1-modified lentiviral vectors, HEK293T, HeLa or KB cells were seeded in a 96-well optical bottom plate (NUNC #165305; Thermo Fisher Scientific) at ∼1 × 104 cells per well. .. Twenty hours afterward, the cells were transduced with 10 μl (∼5 × 10∧8 genomic copies/ml) of concentrated RGD/T1-conjugated or wild-type lentiviral vector containing a luciferase or GFP reporter gene.

    Article Title: Generation of a caged lentiviral vector through an unnatural amino acid for photo-switchable transduction
    Article Snippet: Bioluminescence imaging of living cells For photocleavage experiments and time-dependent live-cell imaging, HEK293T cells were seeded in a 96-well optical bottom plate (NUNC #165305; Thermo Fisher Scientific) at ∼1 × 104 cells per well at 20 h before introduction of 50 μl (∼5 × 10∧8 genomic copies/ml) T0/T1/T2-conjugated lentiviral vectors (with luciferase or GFP as the reporter gene). .. At 72 h after transduction, the culture medium was removed and 50-μl of Bright-Glo reagent (Promega) was added to each well, and the luminescence was detected with an illuminometer (Centro XS3 LB 961; Berthold Technologies, Bad Wildbad, Germany).

    Luciferase:

    Article Title: Generation of a caged lentiviral vector through an unnatural amino acid for photo-switchable transduction
    Article Snippet: Transduction and integration of RGD/T1-modified lentiviral vectors To determine in vitro transduction behavior of the RGD/T1-modified lentiviral vectors, HEK293T, HeLa or KB cells were seeded in a 96-well optical bottom plate (NUNC #165305; Thermo Fisher Scientific) at ∼1 × 104 cells per well. .. Twenty hours afterward, the cells were transduced with 10 μl (∼5 × 10∧8 genomic copies/ml) of concentrated RGD/T1-conjugated or wild-type lentiviral vector containing a luciferase or GFP reporter gene.

    Article Title: Generation of a caged lentiviral vector through an unnatural amino acid for photo-switchable transduction
    Article Snippet: .. Bioluminescence imaging of living cells For photocleavage experiments and time-dependent live-cell imaging, HEK293T cells were seeded in a 96-well optical bottom plate (NUNC #165305; Thermo Fisher Scientific) at ∼1 × 104 cells per well at 20 h before introduction of 50 μl (∼5 × 10∧8 genomic copies/ml) T0/T1/T2-conjugated lentiviral vectors (with luciferase or GFP as the reporter gene). ..

    Stable Transfection:

    Article Title: Melanoma-associated mutants within the serine-rich domain of PAK5 direct kinase activity to mitogenic pathways
    Article Snippet: .. Cell viability assays 30,000 hMELT stable cell lines were seeded in black-walled 96-well plates (Thermo Scientific #165305) coated with poly-L-lysine (Sigma #P1274). .. The next day, cells were treated with either DMSO or temozolomide (Sigma #T2577).

    Cytometry:

    Article Title: CopySwitch—in vivo Optimization of Gene Copy Numbers for Heterologous Gene Expression in Bacillus subtilis
    Article Snippet: .. Plate Reader Measurements Directly after all flow cytometry measurements had been done, 200 μl of each diluted sample were transferred from the flow cytometry tubes into 96-well microwell plates (Nunc™ MicroWell™ #152037, ThermoFisher Scientific, Waltham, MA, USA). .. Optical density was measured at 600 nm in a PHERAstar FSX (BMG Labtech, Ortenberg, Germany).

    Article Title: Generation of a caged lentiviral vector through an unnatural amino acid for photo-switchable transduction
    Article Snippet: Bioluminescence imaging of living cells For photocleavage experiments and time-dependent live-cell imaging, HEK293T cells were seeded in a 96-well optical bottom plate (NUNC #165305; Thermo Fisher Scientific) at ∼1 × 104 cells per well at 20 h before introduction of 50 μl (∼5 × 10∧8 genomic copies/ml) T0/T1/T2-conjugated lentiviral vectors (with luciferase or GFP as the reporter gene). .. For GFP-lentivirus, GFP fluorescence was detected on an inverted fluorescence microscope, and GFP-positive cells were quantified by flow cytometry.

    Incubation:

    Article Title: Reactive astrocytic S1P3 signaling modulates the blood–tumor barrier in brain metastases
    Article Snippet: .. For assessing the passage of these fluorescent molecules, which is directly proportional to the permeability of the BBB/BTB, 100 µL PBS was collected after 45 min incubation into 96-well clear bottom plates (Thermo Scientific, #165305). .. Fluorescence (at 480/590 nm) was measured in SpectraMax M2 (Molecular Devices).

    Article Title: Gemcitabine induces Parkin-independent mitophagy through mitochondrial-resident E3 ligase MUL1-mediated stabilization of PINK1
    Article Snippet: Screening of the chemical library Cells expressing mt-Keima were seeded in 100 µl of media containing 1,000 cells per well in 96-well optical bottom plates (165305, Thermo Scientific) using the Multidrop Combi reagent dispenser (5840300, Thermo Scientific) and cultured at 37 °C for 24 h. Chemical compounds were dissolved in DMSO and added to the cultures at 10 µM using the Biomek NXP automated liquid handler (A16094, Beckman Coulter Life Sciences). .. The plates were incubated for 24 h at 37 °C under 5% CO2 and analyzed using the IN Cell Analyzer 2000 (GE Healthcare Life Sciences).

    Article Title: Melanoma-associated mutants within the serine-rich domain of PAK5 direct kinase activity to mitogenic pathways
    Article Snippet: Cell viability assays 30,000 hMELT stable cell lines were seeded in black-walled 96-well plates (Thermo Scientific #165305) coated with poly-L-lysine (Sigma #P1274). .. On the 7th day, cells were incubated with 0.0036% resazurin (Sigma #R7017) for one hour at 37°C and fluorescence measured in a BioTek Synergy™ HT microplate reader using 540/59 excitation/emission filters. p -values were calculated using multiple t-tests and significance determined by FDR (Q = 5%).

    Article Title: A direct assessment of human prion adhered to steel wire using real-time quaking-induced conversion
    Article Snippet: Briefly, 95 μL of reaction buffer (50 mM PIPES pH 7.0, 500 mM NaCl, 1 mM EDTA and 10 μM Thioflavin T (ThT) including 80 μg/mL of recHamPrP23-231 for 263K-BH, or 100 μg/mL of recHuPrP23-231 for sCJD-BH) were loaded into wells of a 96-well optical-bottom black plate (Thermo Fisher Scientific 265301, MA, USA). .. Ninety-six-well plates were covered with sealing tape (Greiner bio-one 676060, Frickenhausen, Germany) and incubated at 37 °C in a plate reader (Infinite F200 PRO fluorescence plate reader; Tecan, Zurich, Switzerland) with intermittent shaking, consisting of shaking (432 rpm orbital) for 30 sec and no shaking for 30 sec, with a 2-min pause to measure the fluorescence.

    Article Title: The Tubular Sheaths Encasing Methanosaeta thermophila Filaments Are Functional Amyloids *
    Article Snippet: 200 μl samples were loaded into a 96-well Nunc optical bottom plate (catalog no. 265301; Thermo Scientific). .. Immediately afterward, the plate was transferred to a Tecan GENios Pro plate reader and incubated at 37 °C overnight with 30 s of linear shaking at 180 rpm applied every 84 s. The following buffers were used: citric acid (pH 3.0 and 4.0), acetic acid (pH 5.0), MES (pH 6.0), imidazole (pH 7.0), Tris (pH 8.0), and glycine (pH 9.0).

    Expressing:

    Article Title: Gemcitabine induces Parkin-independent mitophagy through mitochondrial-resident E3 ligase MUL1-mediated stabilization of PINK1
    Article Snippet: .. Screening of the chemical library Cells expressing mt-Keima were seeded in 100 µl of media containing 1,000 cells per well in 96-well optical bottom plates (165305, Thermo Scientific) using the Multidrop Combi reagent dispenser (5840300, Thermo Scientific) and cultured at 37 °C for 24 h. Chemical compounds were dissolved in DMSO and added to the cultures at 10 µM using the Biomek NXP automated liquid handler (A16094, Beckman Coulter Life Sciences). ..

    Flow Cytometry:

    Article Title: CopySwitch—in vivo Optimization of Gene Copy Numbers for Heterologous Gene Expression in Bacillus subtilis
    Article Snippet: .. Plate Reader Measurements Directly after all flow cytometry measurements had been done, 200 μl of each diluted sample were transferred from the flow cytometry tubes into 96-well microwell plates (Nunc™ MicroWell™ #152037, ThermoFisher Scientific, Waltham, MA, USA). .. Optical density was measured at 600 nm in a PHERAstar FSX (BMG Labtech, Ortenberg, Germany).

    Article Title: Generation of a caged lentiviral vector through an unnatural amino acid for photo-switchable transduction
    Article Snippet: Bioluminescence imaging of living cells For photocleavage experiments and time-dependent live-cell imaging, HEK293T cells were seeded in a 96-well optical bottom plate (NUNC #165305; Thermo Fisher Scientific) at ∼1 × 104 cells per well at 20 h before introduction of 50 μl (∼5 × 10∧8 genomic copies/ml) T0/T1/T2-conjugated lentiviral vectors (with luciferase or GFP as the reporter gene). .. For GFP-lentivirus, GFP fluorescence was detected on an inverted fluorescence microscope, and GFP-positive cells were quantified by flow cytometry.

    Positive Control:

    Article Title: Reactive astrocytic S1P3 signaling modulates the blood–tumor barrier in brain metastases
    Article Snippet: For assessing the passage of these fluorescent molecules, which is directly proportional to the permeability of the BBB/BTB, 100 µL PBS was collected after 45 min incubation into 96-well clear bottom plates (Thermo Scientific, #165305). .. PBS was used as negative control and coated insert without cells was used as a positive control.

    Cell Culture:

    Article Title: Gemcitabine induces Parkin-independent mitophagy through mitochondrial-resident E3 ligase MUL1-mediated stabilization of PINK1
    Article Snippet: .. Screening of the chemical library Cells expressing mt-Keima were seeded in 100 µl of media containing 1,000 cells per well in 96-well optical bottom plates (165305, Thermo Scientific) using the Multidrop Combi reagent dispenser (5840300, Thermo Scientific) and cultured at 37 °C for 24 h. Chemical compounds were dissolved in DMSO and added to the cultures at 10 µM using the Biomek NXP automated liquid handler (A16094, Beckman Coulter Life Sciences). ..

    Imaging:

    Article Title: Generation of a caged lentiviral vector through an unnatural amino acid for photo-switchable transduction
    Article Snippet: .. Bioluminescence imaging of living cells For photocleavage experiments and time-dependent live-cell imaging, HEK293T cells were seeded in a 96-well optical bottom plate (NUNC #165305; Thermo Fisher Scientific) at ∼1 × 104 cells per well at 20 h before introduction of 50 μl (∼5 × 10∧8 genomic copies/ml) T0/T1/T2-conjugated lentiviral vectors (with luciferase or GFP as the reporter gene). ..

    Protein Concentration:

    Article Title: The Tubular Sheaths Encasing Methanosaeta thermophila Filaments Are Functional Amyloids *
    Article Snippet: Protein concentration was estimated by UV absorbance using the following extinction coefficients for the recombinant MspA from M. thermophila PT: ϵ280 nm = 1.885 cm−1 ·(g/liter)−1 . .. 200 μl samples were loaded into a 96-well Nunc optical bottom plate (catalog no. 265301; Thermo Scientific).

    Recombinant:

    Article Title: The Tubular Sheaths Encasing Methanosaeta thermophila Filaments Are Functional Amyloids *
    Article Snippet: Protein concentration was estimated by UV absorbance using the following extinction coefficients for the recombinant MspA from M. thermophila PT: ϵ280 nm = 1.885 cm−1 ·(g/liter)−1 . .. 200 μl samples were loaded into a 96-well Nunc optical bottom plate (catalog no. 265301; Thermo Scientific).

    MTT Assay:

    Article Title: Cytoprotective effect of a functional antipollutant blend through reducing B[a] P-induced intracellular oxidative stress and UVA exposure
    Article Snippet: .. Chemicals and raw materials/test compounds Used materials included DMEM/F12 (Sigma, D6421), FBS (Merck S0115), trypsin-EDTA (Sigma, T6689), L-glutamine (Biochrom, K0283), gentamycin (Merck A2712), PBS (Merck, L1825), DMSO (Sigma, D2650), MTT (Sigma M5655), and 96-well optical-bottom black plates (Thermo Scientific, 165305). ..

    Fluorescence:

    Article Title: Reactive astrocytic S1P3 signaling modulates the blood–tumor barrier in brain metastases
    Article Snippet: For assessing the passage of these fluorescent molecules, which is directly proportional to the permeability of the BBB/BTB, 100 µL PBS was collected after 45 min incubation into 96-well clear bottom plates (Thermo Scientific, #165305). .. Fluorescence (at 480/590 nm) was measured in SpectraMax M2 (Molecular Devices).

    Article Title: Gemcitabine induces Parkin-independent mitophagy through mitochondrial-resident E3 ligase MUL1-mediated stabilization of PINK1
    Article Snippet: Screening of the chemical library Cells expressing mt-Keima were seeded in 100 µl of media containing 1,000 cells per well in 96-well optical bottom plates (165305, Thermo Scientific) using the Multidrop Combi reagent dispenser (5840300, Thermo Scientific) and cultured at 37 °C for 24 h. Chemical compounds were dissolved in DMSO and added to the cultures at 10 µM using the Biomek NXP automated liquid handler (A16094, Beckman Coulter Life Sciences). .. The fluorescence images of mt-Keima signals in the lysosome, which are specifically excited at 590 nm, were analyzed using the IN Cell Developer Toolbox.

    Article Title: Melanoma-associated mutants within the serine-rich domain of PAK5 direct kinase activity to mitogenic pathways
    Article Snippet: Cell viability assays 30,000 hMELT stable cell lines were seeded in black-walled 96-well plates (Thermo Scientific #165305) coated with poly-L-lysine (Sigma #P1274). .. On the 7th day, cells were incubated with 0.0036% resazurin (Sigma #R7017) for one hour at 37°C and fluorescence measured in a BioTek Synergy™ HT microplate reader using 540/59 excitation/emission filters. p -values were calculated using multiple t-tests and significance determined by FDR (Q = 5%).

    Article Title: A direct assessment of human prion adhered to steel wire using real-time quaking-induced conversion
    Article Snippet: Briefly, 95 μL of reaction buffer (50 mM PIPES pH 7.0, 500 mM NaCl, 1 mM EDTA and 10 μM Thioflavin T (ThT) including 80 μg/mL of recHamPrP23-231 for 263K-BH, or 100 μg/mL of recHuPrP23-231 for sCJD-BH) were loaded into wells of a 96-well optical-bottom black plate (Thermo Fisher Scientific 265301, MA, USA). .. Ninety-six-well plates were covered with sealing tape (Greiner bio-one 676060, Frickenhausen, Germany) and incubated at 37 °C in a plate reader (Infinite F200 PRO fluorescence plate reader; Tecan, Zurich, Switzerland) with intermittent shaking, consisting of shaking (432 rpm orbital) for 30 sec and no shaking for 30 sec, with a 2-min pause to measure the fluorescence.

    Article Title: Generation of a caged lentiviral vector through an unnatural amino acid for photo-switchable transduction
    Article Snippet: Transduction and integration of RGD/T1-modified lentiviral vectors To determine in vitro transduction behavior of the RGD/T1-modified lentiviral vectors, HEK293T, HeLa or KB cells were seeded in a 96-well optical bottom plate (NUNC #165305; Thermo Fisher Scientific) at ∼1 × 104 cells per well. .. Fluorescence images were acquired with a fluorescence microscope (Nikon, Tokyo, Japan) at 48, 72, 96 and 120 h after transduction.

    Article Title: Generation of a caged lentiviral vector through an unnatural amino acid for photo-switchable transduction
    Article Snippet: Bioluminescence imaging of living cells For photocleavage experiments and time-dependent live-cell imaging, HEK293T cells were seeded in a 96-well optical bottom plate (NUNC #165305; Thermo Fisher Scientific) at ∼1 × 104 cells per well at 20 h before introduction of 50 μl (∼5 × 10∧8 genomic copies/ml) T0/T1/T2-conjugated lentiviral vectors (with luciferase or GFP as the reporter gene). .. For GFP-lentivirus, GFP fluorescence was detected on an inverted fluorescence microscope, and GFP-positive cells were quantified by flow cytometry.

    Size-exclusion Chromatography:

    Article Title: A direct assessment of human prion adhered to steel wire using real-time quaking-induced conversion
    Article Snippet: Briefly, 95 μL of reaction buffer (50 mM PIPES pH 7.0, 500 mM NaCl, 1 mM EDTA and 10 μM Thioflavin T (ThT) including 80 μg/mL of recHamPrP23-231 for 263K-BH, or 100 μg/mL of recHuPrP23-231 for sCJD-BH) were loaded into wells of a 96-well optical-bottom black plate (Thermo Fisher Scientific 265301, MA, USA). .. Ninety-six-well plates were covered with sealing tape (Greiner bio-one 676060, Frickenhausen, Germany) and incubated at 37 °C in a plate reader (Infinite F200 PRO fluorescence plate reader; Tecan, Zurich, Switzerland) with intermittent shaking, consisting of shaking (432 rpm orbital) for 30 sec and no shaking for 30 sec, with a 2-min pause to measure the fluorescence.

    Labeling:

    Article Title: Apabetalone (RVX-208) reduces vascular inflammation in vitro and in CVD patients by a BET-dependent epigenetic mechanism
    Article Snippet: Static adhesion assay HUVECs were seeded into black/clear bottom 96-well plates (Thermo Scientific™ Nunc™ MicroWell™ 96-Well Optical-Bottom Plates with Polymer Base, Thermo Scientific cat #165305) at a density of 12,500 cells/well. .. Endothelial cells were washed once in THP-1 media before addition of 200 μl/well of calcein-AM labeled THP-1 at a density of 0.5 × 106 cells/ml for 1 h at 37 °C and 5% CO2 .

    Purification:

    Article Title: The Tubular Sheaths Encasing Methanosaeta thermophila Filaments Are Functional Amyloids *
    Article Snippet: Purified monomers in extraction buffer were desalted using a PD-10 desalting column (GE Healthcare) equilibrated with ddH2 O. .. 200 μl samples were loaded into a 96-well Nunc optical bottom plate (catalog no. 265301; Thermo Scientific).

    Cell Adhesion Assay:

    Article Title: Apabetalone (RVX-208) reduces vascular inflammation in vitro and in CVD patients by a BET-dependent epigenetic mechanism
    Article Snippet: .. Static adhesion assay HUVECs were seeded into black/clear bottom 96-well plates (Thermo Scientific™ Nunc™ MicroWell™ 96-Well Optical-Bottom Plates with Polymer Base, Thermo Scientific cat #165305) at a density of 12,500 cells/well. .. An initial 1-h pre-conditioning was followed by 4 h 2.5 ng/mL TNFα (Peprotech cat #300-01A-50UG, Lot 0607B25 G2516) treatment (in presence of compound or DMSO).

    Microscopy:

    Article Title: Generation of a caged lentiviral vector through an unnatural amino acid for photo-switchable transduction
    Article Snippet: Transduction and integration of RGD/T1-modified lentiviral vectors To determine in vitro transduction behavior of the RGD/T1-modified lentiviral vectors, HEK293T, HeLa or KB cells were seeded in a 96-well optical bottom plate (NUNC #165305; Thermo Fisher Scientific) at ∼1 × 104 cells per well. .. Fluorescence images were acquired with a fluorescence microscope (Nikon, Tokyo, Japan) at 48, 72, 96 and 120 h after transduction.

    Article Title: Generation of a caged lentiviral vector through an unnatural amino acid for photo-switchable transduction
    Article Snippet: Bioluminescence imaging of living cells For photocleavage experiments and time-dependent live-cell imaging, HEK293T cells were seeded in a 96-well optical bottom plate (NUNC #165305; Thermo Fisher Scientific) at ∼1 × 104 cells per well at 20 h before introduction of 50 μl (∼5 × 10∧8 genomic copies/ml) T0/T1/T2-conjugated lentiviral vectors (with luciferase or GFP as the reporter gene). .. For GFP-lentivirus, GFP fluorescence was detected on an inverted fluorescence microscope, and GFP-positive cells were quantified by flow cytometry.

    Plasmid Preparation:

    Article Title: Generation of a caged lentiviral vector through an unnatural amino acid for photo-switchable transduction
    Article Snippet: Transduction and integration of RGD/T1-modified lentiviral vectors To determine in vitro transduction behavior of the RGD/T1-modified lentiviral vectors, HEK293T, HeLa or KB cells were seeded in a 96-well optical bottom plate (NUNC #165305; Thermo Fisher Scientific) at ∼1 × 104 cells per well. .. Twenty hours afterward, the cells were transduced with 10 μl (∼5 × 10∧8 genomic copies/ml) of concentrated RGD/T1-conjugated or wild-type lentiviral vector containing a luciferase or GFP reporter gene.

    Negative Control:

    Article Title: Reactive astrocytic S1P3 signaling modulates the blood–tumor barrier in brain metastases
    Article Snippet: For assessing the passage of these fluorescent molecules, which is directly proportional to the permeability of the BBB/BTB, 100 µL PBS was collected after 45 min incubation into 96-well clear bottom plates (Thermo Scientific, #165305). .. PBS was used as negative control and coated insert without cells was used as a positive control.

    In Vitro:

    Article Title: Reactive astrocytic S1P3 signaling modulates the blood–tumor barrier in brain metastases
    Article Snippet: For assessing the permeability of these in vitro BBB/BTB, after TEER measurement, the transwell inserts with endothelial cells and pericytes were placed in a new 24-well plate containing 1 mL PBS. .. For assessing the passage of these fluorescent molecules, which is directly proportional to the permeability of the BBB/BTB, 100 µL PBS was collected after 45 min incubation into 96-well clear bottom plates (Thermo Scientific, #165305).

    Article Title: The Tubular Sheaths Encasing Methanosaeta thermophila Filaments Are Functional Amyloids *
    Article Snippet: Paragraph title: In Vitro Fibrillation ... 200 μl samples were loaded into a 96-well Nunc optical bottom plate (catalog no. 265301; Thermo Scientific).

    Article Title: Generation of a caged lentiviral vector through an unnatural amino acid for photo-switchable transduction
    Article Snippet: .. Transduction and integration of RGD/T1-modified lentiviral vectors To determine in vitro transduction behavior of the RGD/T1-modified lentiviral vectors, HEK293T, HeLa or KB cells were seeded in a 96-well optical bottom plate (NUNC #165305; Thermo Fisher Scientific) at ∼1 × 104 cells per well. .. Twenty hours afterward, the cells were transduced with 10 μl (∼5 × 10∧8 genomic copies/ml) of concentrated RGD/T1-conjugated or wild-type lentiviral vector containing a luciferase or GFP reporter gene.

    Live Cell Imaging:

    Article Title: Generation of a caged lentiviral vector through an unnatural amino acid for photo-switchable transduction
    Article Snippet: .. Bioluminescence imaging of living cells For photocleavage experiments and time-dependent live-cell imaging, HEK293T cells were seeded in a 96-well optical bottom plate (NUNC #165305; Thermo Fisher Scientific) at ∼1 × 104 cells per well at 20 h before introduction of 50 μl (∼5 × 10∧8 genomic copies/ml) T0/T1/T2-conjugated lentiviral vectors (with luciferase or GFP as the reporter gene). ..

    Staining:

    Article Title: A Method for the Direct Identification of Differentiating Muscle Cells by a Fluorescent Mitochondrial Dye
    Article Snippet: Red fluorescent signal quantification by a plate reader The same number of cells were plated onto 94-well plate (Nalge Nunc International; 165305), and 2 days following seeding, the cells were transferred to DM to induce muscle differentiation. .. At the required time points, the cells were stained with MitoTracker and washed twice with 1XPBS to remove phenol-Red containing medium.

    Article Title: Apabetalone (RVX-208) reduces vascular inflammation in vitro and in CVD patients by a BET-dependent epigenetic mechanism
    Article Snippet: Static adhesion assay HUVECs were seeded into black/clear bottom 96-well plates (Thermo Scientific™ Nunc™ MicroWell™ 96-Well Optical-Bottom Plates with Polymer Base, Thermo Scientific cat #165305) at a density of 12,500 cells/well. .. THP-1 cells were stained with 5 μM calcein-AM (Thermo Fisher Scientific cat # C3100MP) in serum-free RPMI-1640 media at a density of 2.5 × 106 cells/ml for 30 min at 37 °C, 5% CO2 and washed twice in THP-1 media.

    Article Title: Cellular metabolic rates and oxidative stress profiles in primary fibroblast cells isolated from virgin females, reproductively experienced females, and male Sprague‐Dawley rats. Cellular metabolic rates and oxidative stress profiles in primary fibroblast cells isolated from virgin females, reproductively experienced females, and male Sprague‐Dawley rats
    Article Snippet: We used Thermo Scientific™ Nunc™ MicroWell™ 96‐Well Optical‐bottom black chimney plates with polymer base treated with ply‐d‐lysine for all fluorescent stains. .. After staining with each fluorescent stain (below) on a separate plate, cells were imaged using a Tecan infinite measure m200 fluorescent plate reader.

    Article Title: Generation of a caged lentiviral vector through an unnatural amino acid for photo-switchable transduction
    Article Snippet: Transduction and integration of RGD/T1-modified lentiviral vectors To determine in vitro transduction behavior of the RGD/T1-modified lentiviral vectors, HEK293T, HeLa or KB cells were seeded in a 96-well optical bottom plate (NUNC #165305; Thermo Fisher Scientific) at ∼1 × 104 cells per well. .. Nuclei were stained with DAPI (blue).

    Permeability:

    Article Title: Reactive astrocytic S1P3 signaling modulates the blood–tumor barrier in brain metastases
    Article Snippet: .. For assessing the passage of these fluorescent molecules, which is directly proportional to the permeability of the BBB/BTB, 100 µL PBS was collected after 45 min incubation into 96-well clear bottom plates (Thermo Scientific, #165305). .. Fluorescence (at 480/590 nm) was measured in SpectraMax M2 (Molecular Devices).

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  • 99
    Thermo Fisher nunc microwell 96 well optical bottom plates
    Nunc Microwell 96 Well Optical Bottom Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nunc microwell 96 well optical bottom plates/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nunc microwell 96 well optical bottom plates - by Bioz Stars, 2020-04
    99/100 stars
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