nunc maxisorp microwell flat bottom 96 well plates  (Thermo Fisher)


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    Name:
    Nunc MaxiSorp flat bottom
    Description:
    The Nunc MaxiSorp high protein binding capacity 96 well ELISA plates are recommended for use in capturing immunoglobulins found in eBioscience Ready SET Go ELISA kits as well as other matched antibody pairs for ELISA Maxisorp plates have a protein binding capacity of approximately 600 650 ng IgG cm2 Reported ApplicationELISA
    Catalog Number:
    44-2404-21
    Price:
    None
    Applications:
    Protein Assays and Analysis|Protein Biology
    Category:
    Lab Supplies Plastics Glassware
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    Structured Review

    Thermo Fisher nunc maxisorp microwell flat bottom 96 well plates
    The Nunc MaxiSorp high protein binding capacity 96 well ELISA plates are recommended for use in capturing immunoglobulins found in eBioscience Ready SET Go ELISA kits as well as other matched antibody pairs for ELISA Maxisorp plates have a protein binding capacity of approximately 600 650 ng IgG cm2 Reported ApplicationELISA
    https://www.bioz.com/result/nunc maxisorp microwell flat bottom 96 well plates/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nunc maxisorp microwell flat bottom 96 well plates - by Bioz Stars, 2020-07
    90/100 stars

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    Enzyme-linked Immunosorbent Assay:

    Article Title: Development and validation of a house finch interleukin-1β (HfIL-1β) ELISA system
    Article Snippet: .. Enzyme-linked Immunosorbent assay (ELISA) To develop the ELISA system for HfIL-1β, we adopted a direct ELISA approach using two different coating methods, carbonate and dehydration, on Nunc MaxiSorp® flat-bottom 96-well plates (Thermo Scientific, IL). ..

    Article Title: A sandwich enzyme-linked immunosorbent assay for the quantitation of human plasma ferritin
    Article Snippet: .. To do the ELISA Nunc MaxiSorp® flat-bottom 96 well plates (ThermoFisher Scientific 442404) were coated with 100 μl/well goat anti-ferritin polyclonal antibody (Abcam ab33574) diluted to 367 ng/mL in phosphate buffered saline (PBS), overnight at 4 °C. .. The following day, the plates were washed with wash buffer (0.05% Tween20® in PBS) and then blocked with 200 μl/well of reagent diluent (1% Bovine Serum Albumin (Sigma A7906), 0.05% Tween20® in PBS) for 1 h at room temperature.

    Article Title: IgD class switching is initiated by microbiota and limited to mucosa-associated lymphoid tissue in mice
    Article Snippet: .. For ELISA analysis of antigen-specific IgM and IgG responses, Nunc MaxiSorp flat-bottom 96-well microplates (Thermo Fisher Scientific) were coated with 5 µg/mL NP8 -BSA (Biosearch Technologies) at 4 °C overnight. .. The plates were then washed four times with washing buffer [0.05% (vol/vol) Tween-20 in PBS] using a BioTek microplate washer, then blocked with 1% (vol/vol) BSA for 1 h at room temperature.

    Article Title: LXR Alpha Restricts Gammaherpesvirus Reactivation from Latently Infected Peritoneal Cells
    Article Snippet: .. IFN-γ concentrations were determined using the IFN-γ ELISA MAX Deluxe set (BioLegend, San Diego, CA) according to the manufacturer’s instructions, using Nunc MaxiSorp flat-bottom plates (Thermo Fisher Scientific, Waltham, MA). ..

    Article Title: Directed evolution of the forkhead-associated domain to generate anti-phosphospecific reagents by phage-display
    Article Snippet: .. To set up the ELISA, biotinylated peptides (100 μL, 5 μg/mL) were immobilized on Nunc MaxiSorp flat-bottom 96 well plates (Thermo Fisher Scientific) via NeutrAvidin™ Biotin binding protein (100 μL, 10 μg/mL; Thermo Fisher Scientific) and blocked with 2% skim milk in Phosphate Buffered Saline (PBS: 0.14 M Sodium Chloride, 0.003 M Potassium Chloride, 0.002 M Potassium Phosphate, and 0.01 M Sodium Phosphate; pH 7.4). ..

    Article Title: Molecular Landscape of Anti-Drug Antibodies Reveals the Mechanism of the Immune Response Following Treatment With TNFα Antagonists
    Article Snippet: .. To test the functionality of the produced mAb-F(ab')2 , 96 ELISA plates (Nunc MaxiSorp™ flat-bottom, Thermo Fisher Scientific) were coated with 1 μg/ml of rhTNFα in PBS and incubated at 4°C O.N. .. ELISA plates were then washed three times with PBST and blocked with 300 μl of 2% w/v BSA in PBS for 1 h at 37°C.

    Produced:

    Article Title: Molecular Landscape of Anti-Drug Antibodies Reveals the Mechanism of the Immune Response Following Treatment With TNFα Antagonists
    Article Snippet: .. To test the functionality of the produced mAb-F(ab')2 , 96 ELISA plates (Nunc MaxiSorp™ flat-bottom, Thermo Fisher Scientific) were coated with 1 μg/ml of rhTNFα in PBS and incubated at 4°C O.N. .. ELISA plates were then washed three times with PBST and blocked with 300 μl of 2% w/v BSA in PBS for 1 h at 37°C.

    Incubation:

    Article Title: Ezh2 programs TFH differentiation by integrating phosphorylation-dependent activation of Bcl6 and polycomb-dependent repression of p19Arf
    Article Snippet: .. In brief, Nunc MaxiSorp flat-bottom 96-well plate (eBiosciences) was coated with 1 µg/ml Imject mcKLH (Thermo Fisher Scientific) overnight, and then incubated with serially diluted serum samples. .. The KLH-specific IgG was detected by Horseradish peroxidase (HRP)-conjugated goat-anti-mouse IgG (H+L) secondary antibody (Thermo Fisher Scientific) coupled with TMB substrate (BD Biosciences).

    Article Title: Molecular Landscape of Anti-Drug Antibodies Reveals the Mechanism of the Immune Response Following Treatment With TNFα Antagonists
    Article Snippet: .. To test the functionality of the produced mAb-F(ab')2 , 96 ELISA plates (Nunc MaxiSorp™ flat-bottom, Thermo Fisher Scientific) were coated with 1 μg/ml of rhTNFα in PBS and incubated at 4°C O.N. .. ELISA plates were then washed three times with PBST and blocked with 300 μl of 2% w/v BSA in PBS for 1 h at 37°C.

    Direct ELISA:

    Article Title: Development and validation of a house finch interleukin-1β (HfIL-1β) ELISA system
    Article Snippet: .. Enzyme-linked Immunosorbent assay (ELISA) To develop the ELISA system for HfIL-1β, we adopted a direct ELISA approach using two different coating methods, carbonate and dehydration, on Nunc MaxiSorp® flat-bottom 96-well plates (Thermo Scientific, IL). ..

    Binding Assay:

    Article Title: Directed evolution of the forkhead-associated domain to generate anti-phosphospecific reagents by phage-display
    Article Snippet: .. To set up the ELISA, biotinylated peptides (100 μL, 5 μg/mL) were immobilized on Nunc MaxiSorp flat-bottom 96 well plates (Thermo Fisher Scientific) via NeutrAvidin™ Biotin binding protein (100 μL, 10 μg/mL; Thermo Fisher Scientific) and blocked with 2% skim milk in Phosphate Buffered Saline (PBS: 0.14 M Sodium Chloride, 0.003 M Potassium Chloride, 0.002 M Potassium Phosphate, and 0.01 M Sodium Phosphate; pH 7.4). ..

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  • 90
    Thermo Fisher nunc maxisorp microwell flat bottom 96 well plates
    Nunc Maxisorp Microwell Flat Bottom 96 Well Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nunc maxisorp microwell flat bottom 96 well plates/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nunc maxisorp microwell flat bottom 96 well plates - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

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